• Journal of Plant Biotechnology
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• v.45 no.3
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• pp.250-256
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• 2018

To determine whether metabolite fingerprinting for whole cell extracts based on Fourier transform infrared spectroscopy (FT-IR) can be used to discriminate and compare metabolic equivalence, standard medicinal parts of Cynanchum wilfordii (Maxim.) Hemsl. and their adventitious roots were subjected to FT-IR. The principal component analysis (PCA) and partial least square discriminant analysis (PLS-DA) from FT-IR spectral data showed that whole metabolic pattern from the adventitious root of Cynanchum wilfordii was highly similar to its standard medicinal parts. These results clearly showed that mass proliferation of adventitious roots could be applied for the novel supply of standard medicinal parts of medicinal plants. Furthermore, FT-IR spectroscopy combined with multivariate analysis established in this study could be applied as an alternative tool for discriminating of whole metabolic equivalence from standard medicinal parts. Thus, it is proposed that these metabolic discrimination systems from the adventitious root of Cynanchum wilfordii could be applied for metabolic standardization of in vitro grown Cynanchum wilfordii.

• Progress in Medical Physics
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• v.15 no.2
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• pp.94-99
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• 2004

Radiochromic film has several advantages; high spatial resolution, relatively low spectral sensitivity, near tissue equivalence and requires no special development procedure. The object of this study was to measure the anisotropy of an Ir-192 source (microSelectron manufactured by Nucletron) in a few mm regions from the source, using the GafChromic film. The GafChromic film was calibrated in the range of 0∼105 Gy, using a 4 MV photon beam, and the anisotropy function measured in an acrylic phantom using the GafChroimic film. The data obtained gave agreement to within 4.4% of the Monte Calro calculation, by J. F. Williamson, at a radial distance of 2.5 mm with polar angles of 50 to 130$^{\circ}$, while a maximum deviation of 17.6% was observed at angles near 140$^{\circ}$and agreement within 3.7% at a radial distance of 5 mm at polar angles between 35 to 150$^{\circ}$ and a maximum deviation of 7.6% was observed at angles near 30$^{\circ}$. A GafChromic film can be used as a more efficient detector for measuring the anisotropy of an HDR $^{192}$ Ir source at close distances than any other detector.