• 대한의생명과학회지
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• 제10권2호
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• pp.65-73
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• 2004

I report that single-stranded antisense as a part of large circular (LC-) genomic DNA of recombinant M13 phage exhibits enhanced stability, sequence specific antisense activity, and no need for target site search. A cDNA fragment (708 bp) of rat TNF-$\alpha$ was inserted into a phagemid vector, and TNF-$\alpha$ antisense molecules (TNF$\alpha$-LCAS) were produced as single-stranded circular DNA. When introduced into a rat monocyte/macrophage cell line, WRT7/P2, TNF$\alpha$-LCAS was able to ablate LPS-induced TNF-$\alpha$ mRNA to completion. The antisense effect of TNF$\alpha$-LCAS was shown to be sequence-specific because expressions of three control genes ($\beta$-actin, GAPDH and IL-1$\beta$) were not significantly altered by the antisense treatment. Further, TNF$\alpha$-LCAS was found to be highly efficacious as only 0.1 $\mu$g (0.24 nM) of TNF$\alpha$-LCAS was sufficient to block TNF-$\alpha$ expression in 1$\times10^5$ WRT7/P2 cells. I have also observed specific antisense activity in reduction of NF-$\kappa$B gene expression. The results suggest that an antisense sequence as a part of single-stranded circular genomic DNA has a specific antisense activity.

• 펄프종이기술
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• 제46권1호
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• pp.11-17
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• 2014

This study analyzed the effects of specific factors on the fluorescence index of the fluorescent whitening agent (FWA) that is used for internal addition. The specific factors were identified by the literature review, and a statistical survey was carried out to analyze the effects of those factors on the fluorescence index. Bulk, grammage, fillers and internal sizing agents were selected as the specific factors. The paper samples were prepared with internal FWA in a laboratory. The fluorescence indices of the model papers were measured, and p-values of the specific factors on the fluorescence index were calculated by the SPSS program. Most of p-values were greater than 0.05, so the specific factors do not have a significant impact on the internal FWA fluorescence index.

• Journal of Microbiology and Biotechnology
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• 제9권2호
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• pp.184-190
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• 1999

The use of the polymerase chain reaction (PCR) method was described using two sets of primers based on the ceuN gene (JEJ 1 and JEJ 2) which encodes a protein involved in siderophore transport and 16S rRNA gene (pA and pB) for the sensitive and specific detection of enteropathogen Campylobacter jejuni. Six oligonucleotides were utilized in an amplification experiment and PCR products of predicted sizes were generated from whole cells and boiled cell lysates at the same intensity. Two sets of the primer pairs, JEJ and pAB, were specific enough for all C. jejuni strains tested for the direct use of whole cells without DNA extraction or lysis steps. In the PCR using the pAB primer pair, the detection limit, as determined by the ethidium bromide staining of the amplification products on agarose gels, was at the level of $10^1$ bacteria cells or less in both the pure culture and artificially inoculated milk and chicken enrichment samples, whereas the detection limit with the JEJ primer pair was relatively low, i.e. $10^3$ cells or more in the same PCR samples. The PCR method using either a primer JEJ or pAB was both repeatable and specific for the detection of C. jejuni in food. This method is simply completed within 4 h.

• Journal of Microbiology and Biotechnology
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• 제27권5호
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• pp.909-915
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• 2017

Bifidobacterium bifidum BGN4 (BGN4) has many proven beneficial effects, including antiallergy and anticancer properties. It has been commercialized and used in several probiotic products, and thus strain-specific identification of this strain is very valuable for further strain-dependent physiological study. For this purpose, we developed novel multiplex polymerase chain reaction (PCR) primer sets for strain-specific detection of BGN4 in commercial products and fecal samples of animal models. The primer set was tested on seven strains of B. bifidum and 75 strains of the other Bifidobacterium species. The BGN4-specific regions were derived using megaBLAST against genome sequences of various B. bifidum databases and four sets of primers were designed. As a result, only BGN4 produced four PCR products simultaneously whereas the other strains did not. The PCR detection limit using BGN4-specific primer sets was $2.8{\times}10^1CFU/ml$ of BGN4. Those primer sets also detected and identified BGN4 in the probiotic products containing BNG4 and fecal samples from a BGN4-fed animal model with high specificity. Our results indicate that the PCR assay from this study is an efficient tool for the simple, rapid, and reliable identification of BGN4, for which probiotic strains are known.

• 정보처리학회논문지D
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• 제11D권4호
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• pp.951-958
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• 2004

본 논문에서는 GPRS망으로 이동한 이동 ISP 가입자가 GPRS망을 접속하여 무선 인터넷 서비스를 받을 때 필요한 홈 ISP망의 동적 내 주소할당에 대한 문제점을 살펴보고 그 해결 방안으로 홈 ISP망 내 특수(specific) DHCP Relay를 개발하여 운영하는 방법을 제시했다. 실제 GPRS core network testbed와 ISP망을 구축하고 특수 DHCP Relay를 개발해서 GPRS망으로 이동한 이동 ISP 가입자에게 홈 ISP망의 동적 IP 주소 할당을 과정과 결과를 시뮬레이션을 통해 확인하였다.

• The Korean Journal of Ceramics
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• 제4권3호
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• pp.245-248
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• 1998

Porous carbon fiber composites (CFCs) having variable specific surface area ranging 35~1150 $\m^2$/g were reacted to produce silicon carbide fiber composites with SiO vapor generated from a mixture of Si and $SiO_2$ at 1673 K for 2 h under vacuum. Part of SiO vapor generated during conversion process condensed on to the converted fiber surface as amorphous silica. Chemical analysis of the converted CFCs resulting from reaction showed that the products contained 27~90% silicon carbide, 7~18% amorphous silica and 3~63% unreacted carbon, and the composition depended on the specific carbide, 7~18% amorphous silica and 3~63% unreacted carbon, and the composition depended on the specific surface area of CFCs. CFC of higher specific surface area yielded higher degree of conversion of carbon to silicon and conversion products of lower mechanical strength due to occurrence of cracks in the converted caron fiber. As the conversion of carbon to silicon carbide proceeded, pore size of converted CFCs increased as a result of growth of silicon carbide crystallites, which is also linked to the crack formation in the converted fiber.

• 한국항공우주학회지
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• 제37권12호
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• pp.1217-1224
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• 2009

고정익 항공기가 3차원의 복잡한 경로를 추종하기 위해 필요한 비교적 간단하며 효과적인 유도 제어 방법을 제시한다. 소개되는 방법은 비선형 경로 추종 유도 기법을 외부 루프로 사용한다. 외부 루프는 원하는 경로와 함께 항공기의 현재 위치와 속도를 바탕으로 비행 경로를 변화하기 위한 가속도 명령을 생성한다. 가속도 명령은 중력과 벡터적으로 결합되어 Specific Force Acceleration을 만든다. 이렇게 생성된 Specific Force Acceleration은 내부 루프를 위한 명령으로 쓰이는데, 이는 항공기가 가속도 자체보다는 Specific Force Acceleration을 더 직접적으로 제어할 수 있기 때문이다. 나아가 배면 비행이나 Slow Roll, Knife-Edge 등과 같은 옆미끄럼짐 기동을 하기 위해 필요한 롤 자세 제어 기법도 제시한다. 마지막으로 표준이 되는 여러 가지 곡예비행 경로들에 대한 시뮬레이션을 수행함으로써 제시된 기법의 성능을 검증한다.

• International Journal of Industrial Entomology and Biomaterials
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• 제23권1호
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• pp.137-141
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• 2011

A previous data was provided information for tissuespecific expression genes by means of whole-genome oligonucleotide microarray in the silkworm. We analyzed the tissue-specific expression patterns in the hemocyte tissue on 5 days of 5th instar larvae during the development of $B.$ $mori$. Total 5 candidates pick out from the $Bombyx$ $mori$ Microarray Database (BmMDB; http://silkworm.swu.edu.cn/microarray). To verify the hemocyte-specific expression, we analyzed by semi-quantitative and real-time quantitative RT-PCR using the highly expressed endogenous $Actin$ RNA as an intrinsic reference. In this study, we confirmed that one gene-sw17255- out of 5 candidates expressed in the hemocyte tissue, which was consistent with the previous data. Circulating hemocytes in the body fluid of the $B.$ $mori$ are most powerful target organ for producing biomaterials. We need further studies to find hemocyte-specific promoter region from sw17255 gene. Finally, this result can be applied in creating transgenic silkworms as a biomedical insect.

• Nuclear Engineering and Technology
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• 제34권6호
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• pp.586-595
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• 2002

The Seismic probabilistic risk assessment (SPRA) or seismic margin assessment (SMA) have been used for the seismic safety evaluation of nuclear power plant structures and equipments. For the SPRA or SMA, the reference response spectrum should be defined. The site-specific median spectrum has been generally used for the seismic fragility analysis of structures and equipments in a Korean nuclear power plant Since the site-specific spectrum has been developed based on the peak ground motion parameter, the site-specific response spectrum does not represent the same probability of exceedance over the entire frequency range of interest. The uniform hazard spectrum is more appropriate to be used in seismic probabilistic risk assessment than the site- specific spectrum. A method for modifying the seismic fragility parameters that are calculated based on the site-specific median spectrum is described. This simple method was developed to incorporate the effects of the uniform hazard spectrum. The seismic fragility parameters of typical NPP components are modified using the uniform hazard spectrum. The modification factor is used to modify the original fragility parameters. An example uniform hazard spectrum is developed using the available seismic hazard data for the Korean nuclear power plant (NPP) site. This uniform hazard spectrum is used for the modification of fragility parameters.

• 한국태양에너지학회:학술대회논문집
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• 한국태양에너지학회 2011년도 춘계학술발표대회 논문집
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• pp.235-240
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• 2011

Small hydropower resources for five major river systems have been studied. The model, which can predict flow duration characteristic of stream, was developed to analyze the variation of inflow caused from rainfall condition. And another model to predict hydrologic performance for small hydropower(SHP) plants is established. Monthly inflow data measured at Andong dam were analyzed. The predicted results from the developed models in this study showed that the data were in good agreement with measured results of long term inflow at Andong darn. It was found that the models developed in this study can be used to predict the available potential and technical potential of SHP sites effectively. Based on the models developed in this study, the hydrologic performance for small hydropower sites located in river systems have been analyzed. The results show that the hydrologic performance characteristics of SHP sites have some difference between the river systems. Especially, the specific design flowrate and specific output of SHP sites located on North Han river and Nakdong river systems have large difference compared with other river systems.