• The Journal of the Convergence on Culture Technology
• /
• v.10 no.3
• /
• pp.873-878
• /
• 2024

We collected rat feces by group period after oral administration of fructooligosaccharides and fruit and vegetable complex extracts for 2 weeks in the Sprague-Dawley rat model of loperamide-induced constipation and analyzed trends in changes in the intestinal microbiome. Microbial composition analysis was performed on Fractoologosaccharide and fruit and vegetable complex extracs(FVCE), by 16S rDNA cloning and pyrosequencing to obtain basic data for the standardization and systematization of the FVCE manufacturing process. Microbial analysis of the prokaryotic community revealed a slight difference in microbial verrucomicrobiota was dominant at the phylum level. At the genus level, prevotella and muribaculaceae showed further differences at the species level. These results suggest that the microbial community used affects the quality of fruit and vegetable complex extracs(FVCE) produced. Thus, a stable microbial community must be maintained for the production of fruit and vegetable complex extracs(FVCE) with consistent quality.

• Korean journal of applied entomology
• /
• v.63 no.1
• /
• pp.77-80
• /
• 2024

Aedes albopictus, a common species in the Republic of Korea, is internationally known as a major vector for various diseases, and it is well-adapted to urban environments. Recent insect outbreaks in urban areas, attributed to climate change and urban heat islands, have increased the necessity of researching the effects on mosquito populations. This study analyzed climate data from 25 Automatic Weather System (AWS) stations in Seoul, identifying urban areas with pronounced heat island effects and suburban areas with milder effects. Nine urban heat island conditions were established based on this analysis, under which the hatching rates of Ae. albopictus were examined. The results revealed an increase in hatching rates correlating with the intensity of the urban heat island effect. Regression analysis further indicated that this trend accelerates as the strength of the heat island effect increases. This study suggests that temperature variations resulting from urban heat island phenomena can significantly influence the hatching rates of Ae. albopictus.

• Animal Bioscience
• /
• v.37 no.4
• /
• pp.640-654
• /
• 2024

Objective: The purpose of this study was to explore the effect of sodium salicylate (SS) on semen preservation and metabolic regulation in goats. Methods: Under the condition of low temperature, SS was added to goat semen diluent to detect goat sperm motility, plasma membrane, acrosome, antioxidant capacity, mitochondrial membrane potential (MMP) and metabonomics. Results: The results show that at the 8th day of low-temperature storage, the sperm motility of the 20 μM SS group was 66.64%, and the integrity rates of the plasma membrane and acrosome were both above 60%, significantly higher than those of the other groups. The activities of catalase and superoxide dismutase in the sperm of the 20 μM SS group were significantly higher than those of the control group, the contents of reactive oxygen species and malondialdehyde were significantly lower than those in the control group, the MMP was significantly higher than that in the control group, and the contents of Ca²⁺ and total cholesterol were significantly higher than those in the control group. Through metabonomics analysis, there were significant metabolic differences between the control group and the 20 μM SS group. Twenty of the most significant metabolic markers were screened, mainly involving five metabolic pathways, of which nicotinic acid and nicotinamide metabolic pathways were the most significant. Conclusion: The results indicate that SS can effectively improve the low-temperature preservation quality of goat sperm.

• Journal of Korean Society of Forest Science
• /
• v.38 no.1
• /
• pp.33-45
• /
• 1978

The purpose of present study is to analyze the genetic variation of natural stand of Pinus densiflora. In 1975 following after the selection of 1974, twenty trees from each of three natural populations of the species were selected and their open-pollinated seeds were collected, and the locations and conditions of the populations ate presented in table 1, 2 and figure 1. Some morphological traits of the populations were already detailed in our second report of this series, in which Myong-Ju and Ul-Jin populations were regarded to be superior phenotypically to suweon population. The morphological traits of cone, seed and seed-wing, and also the growth performances and needle characters of the seedling were observed in the present study according to the previous methods. The results obtained are summarized as follows; 1. The meteorological data obtained by averaging the records of 30 year period (1931~1960) measured from the nearest meteorological stations to each population are shown in fig.2, 3, 4. The distributional patterns of investigated climate factors are generally considered to be similar among the locations. However, the precipitation density during growing season and the air temperature during dormant season on Suweon area, population 6, were quite different from those of the other areas. 2. The measurements of fresh cone weight, length, diameter and cone index, i.e., length to diameter ratio are presented in table 7. As shown in table 7, all these traits except for cone diameter seem to be highly significant in population differences and family differences within population. 3. The morphological traits of seed and seed-wing are detailed in table 8, 9, and highly significant differences are recognized among the populations and the families within population in seed-wing length, seed-wing index, seed weight, seed-length and seed index but not among the populations in the other observed traits. The values of correlation coefficient between the characters of cone and seed are given in table 10 and the positive significant correlations can be observed in the most parts of the compared traits. 4. Significant statistical differences among populations and families within population are observed in the growth performances of 1-0 and 1-1 seedling height of these progenies. But the differences in root collar diameter are shown only among families within population. As shown in table 13, the most parts of correlations are not significant statistically between the growth performances of seedling and the seed characters. 5. The number of stomata row on both sides of needle and the serration density were measured in the seedlings from each of the families of the three populations. As shown in table 15, statistical differences are considered to be significant among the populations and among the families within population in serration density but not among the populations in stomata row on both sides of the needle. The results differ from those of the third report of this series. Even if one of the reason seems to be the diversity of selected populations, it could not be confirmed definitely. The correlations between progenies and parents are not generally observed in the investigated traits of needle as shown in table 16.

• Korean Journal of Environment and Ecology
• /
• v.31 no.2
• /
• pp.152-165
• /
• 2017

The greater long-tail hamster, Tscherskia triton, is widely distributed in Northern China, Korea and adjacent areas of Russia. Except for its distribution, biological characteristics related to life history, behavior, and ecological influences for this species are rarely studied in Korea. This study was conducted to obtain biological information on breeding, growth and development that are basic to species-specific studies. The study adopted laboratory management of a breeding programme for T. triton collected in Jeju Island from March, 2015 to December, 2016. According to the study results, the conception rate was 31.67% and the mice in the large cages had a higher rate of conception than those in the small cages (56.7 vs. 6.7%). The gestation period was $22{\pm}1.6days$ (ranges from 21 to27 days), and litter size ranged from 2 to 7, with a mean of $4.26{\pm}1.37$ in the species. The minimum age for weaning was between $19.2{\pm}1.4days$ (range of 18-21 days). There were no significant differences by sex between mean body weight and external body measurements at birth. However, a significant sexual difference was found from the period of weaning (21 days old) in head and body length, as well as tail length (HBL-weaning, $106.50{\pm}6.02$ vs. $113.34{\pm}4.72mm$, p<0.05; HBL-4 months, $163.93{\pm}5.42$ vs. $182.83{\pm}4.32mm$, p<0.05; TL-4 months, $107.23{\pm}3.25$ vs. $93.95{\pm}2.15mm$, p<0.05). Gompertz and Logistic growth curves were fitted to data for body weight and lengths of head and body, tail, ear, and hind foot. In two types of growth curves, males exhibited greater asymptotic values ($164.840{\pm}7.453$ vs. $182.830{\pm}4.319mm$, p<0.0001; $163.936{\pm}5.415$ vs. $182.840{\pm}4.333mm$, p<0.0001), faster maximum growth rates ($1.351{\pm}0.065$ vs. $1.435{\pm}0.085$, p<0.05; $2.870{\pm}0.253$ vs. $3.211{\pm}0.635$, p<0.05), and a later age of maximum growth than females in head and body length ($5.121{\pm}0.318$ vs. $5.520{\pm}0.333$, p<0.05; $6.884{\pm}0.336$ vs. $7.503{\pm}0.453$, p<0.05). However, females exhibited greater asymptotic values ($105.695{\pm}5.938$ vs. $94.150{\pm}2.507mm$, p<0.001; $111.609{\pm}14.881$ vs. $93.960{\pm}2.150mm$, p<0.05) and longer length of inflection ($60.306{\pm}1.992$ vs. $67.859{\pm}1.330mm$, p<0.0001; $55.714{\pm}7.458$ vs. $46.975{\pm}1.074mm$, p<0.05) than males in tail length. These growth rate constants, viz. the morphological characters and weights of the males and females, were similar to each other in two types of growth curves. These results will be used as necessary data to study species specificity of T. triton with biological foundations.

• Journal of The Korean Society of Grassland and Forage Science
• /
• v.11 no.4
• /
• pp.236-243
• /
• 1991

A field experiment was conducted during the growing season of 1985 to determine the effects of slope orientation, north and south facing exposures and three different altitudes, 800, 1,000 and 1,200m a.s.1. on the dry matter yield, forage quality and botanical composition of alpine pasture. 1. Total dry matter yield of alpine pasture was influenced by slope orientation and altitude. An average forage yield of both exposures at 1,000m a.s.1. was 9,301kg/ha compared with 10,274kg/ha at 800m a.s.1. and the productivity express in relative percentage decreased by 9.5% per 200m attitudinal increase. 2. An average forage yield of both altitudes, 800 and 1,000m a.s.1. on the south facing exposure was 21.5% higher than that on the north exposure. 3. Crude protein concentration of forage grown on both exposures was lowest at 800m a.s.1. increasing gradually as the altitude increased, but crude fiber concentration decreaced in a manner opposite to that of crude protein. 4. Forage grown on the north facing exposure had a higher concentration of crude protein than that grown on the south facing exposure at the same altitude, but lower concentraion of crude fiber. 5. Orchardgrass and timothy were two main species on both exposures at 800m a.s.1. but timothy was only dominant species at 1,000 and 1,200m a.s.1. and performed well up to 1,200m a.s.1. in alpine area. The economic production limit of orchardgrass would be about 800m a.s.1. in the alpine pasture. 6. No soil property differences were found between the north and south facing exposures as well as three different altitudes in the alpine pasture. It seemed that the previous pasture fertilization of the experimental field completely overshadowed any soil property differences from slope orientation and altitude.

• Korean Journal of Weed Science
• /
• v.31 no.2
• /
• pp.134-145
• /
• 2011

The objectives of this study were to investigate fitness difference in growth and rice yield in herbicide-transgenic rice overexpressing Myxococcus xanthus and Arabidopsis thaliana protoporphyrinogen oxidase (Protox) genes and non-transgenic rice. We also aimed to determine whether these fitness differences are related to ALA synthesizing capacity, accumulation of terapyrroles, reactive oxygen species, lipid peroxidation, and antioxidative enzymes at different growth stages of rice. Plant height of the transgenic rice overexpressing M. xanthus (MX) and A. thaliana (AP37) Protox genes at 43, 50, and 65 days after transplanting (DAT) was significantly lower than that of WT. Number of tiller of PX as well as MX and AP37 at 50 and 65 DAT was significantly lower than that of WT. At harvest time, culm length and yield of MX, PX and AP37 and rice straw weight of MX and AP37 were significantly low compared with WT. The reduction of yield in MX, PX, and AP37 was caused by spikelets per panicle and 1000 grain weight, ripened grain, spikelets per panicle, 1000 grain weight, and ripened grain, respectively. On the other hand, 135 the reduction of yield in MX, PX, and AP37 was also observed in another yearly variation experiment. The reduction of rice growth in MX, PX, and AP37 was observed in seedling stage as well as growth duration in field. There were no differences in tetrapyrrole intermediate Proto IX, Mg-Proto IX and Mg-Proto IX monomethyl ester, reactive oxygen species ($H_2O_2$ and ${O_2}^-$), MDA, antioxidative enzymes (SOD, CAT, POX, APX, and GR) and chlorophyll between transgenic lines and wild type, indicating that accumulated tetrapyrrole intermediate and other parameters were not related to growth reduction in transgenic rice. However, ALA synthesizing capacity in MX, PX, and AP37 at one day after exposure to light and 52 DAT was significantly lower than that of WT. Further study is required to elucidate the mechanisms underlying the growth and yield difference between transgenic and WT lines.

• Journal of Aquaculture
• /
• v.17 no.1
• /
• pp.69-80
• /
• 2004

The objective of the present study was to analyze genetic distances, variation and characteristics of individuals in rainbow trout, Oncorhynchus mykis using amplified fragment length polymorphism (AFLP) method as molecular genetic technique, to detect AFLP band patterns as genetic markers, and to compare the efficiency of agarosegel electrophoresis (AGE) and polyacrylamide gel electrophoresis (PAGE), respectively. Using 9 primer combinations, a total of 141 AFLP bands were produced, 108 bands (82.4%) of which were polymorphic in AGE. In PAGE, a total of 288 bands were detected, and 220 bands (76.4%) were polymorphic. The AFLP fingerprints of AGE were different from those of PAGE. Separation of the fragments with low molecular weight and genetic polymorphisms revealed a distinct pattern in the two gel systems. In the present study, the average bandsharing values of the individuals between two populations apart from the geographic sites in Kangwon-do ranged from 0.084 to 0.738 of AGE and PAGE. The bandsharing values between individuals No.9 and No. 10 showed the highest level within population, whereas the bandsharing values between individuals No.5 and No.7 showed the lowest level. As calculated by bandsharing analysis, an average of genetic difference (mean$\pm$SD) of individuals was approximately 0.590$\pm$0.125 in this population. In AGE, the single linkage dendrogram resulted from two primers (M11+H11 and M13+H11), indicating six genetic groupings composed of group 1 (No.9 and 10), group 2 (No. 1, 4, 5, 7, 10, 11, 16 and 17), group 3 (No. 2, 3, 6, 8, 12, 15 and 16), group 4 (No.9, 14 and 17), group 5 (No. 13, 19, 20 and 21) and group 6 (No. 23). In AGE, the genetic distances among individuals of between-population ranged from 0.108 to 0.392. In AGE, the shortest genetic distance (0.108) displaying significant molecular differences was between individuals No.9 and No. 10. Especially, the genetic distance between individuals No. 23 and the remnants among individuals within population was highest (0.392). Additionally, in the cluster analysis using the PAGE data, the single linkage dendrogram resulted from two primers (M12+H13 and M11+H13), indicating seven genetic groupings composed of group 1 (No. 15), group 2 (No. 14), group 3 (No. 11 and 12), group 4 (No.5, 6, 7, 8, 10 and 13), group 5 (No.1, 2, 3 and 4), group 6 (No.9) and group 7 (No. 16). By comparison with the individuals in PAGE, genetic distance between No. 10 and No. 7 showed the shortest value (0.071), also between No. 16 and No. 14 showed the highest value (0.242). As with the PAGE analysis, genetic differences were certainly apparent with 13 of 16 individuals showing greater than 80% AFLP-based similarity to their closest neighbor. The three individuals (No. 14, No. 15 and No. 16) of rainbow trout between two populations apart from the geographic sites in Kangwon-do formed distinct genetic distances as compared with other individuals. These results indicated that AFLP markers of this fish could be used as genetic information such as species identification, genetic relationship or analysis of genome structure, and selection aids for genetic improvement of economically important traits in fish species.

• Research in Plant Disease
• /
• v.10 no.2
• /
• pp.105-111
• /
• 2004

Mycelial growth and days required for sclerotial formation(DRSF) in vitro, and disease incidence of infected plants in the field were investigated to evaluate disease resistance of nine garlic cultivars against two species of Sclerotium causing garlic white rot. There were differences among mycelial growth and sclerotial formation of two white rot pathogens on different garlic cultivars in vitro. Mycelial growth showed the highest level on the clove of 'Daeseo'. Sclerotia of two white rot pathogen were formed the earliest on the clove of 'Kodang' and the latest on the clove of 'Namdo'. In field trial all of nine garlic cultivars were highly susceptible to Sclerotium cepivorum and cold-type garlics were less susceptible than warm-type garlics. Meanwhile garlic cultivars used showed differences among resistant responses to Sclerotium sp., forming large sclerotia. All of warm-type garlics showed highly susceptible response, but three cold-type garlics, 'Seosan', 'Danyang' and 'Yechon' showed moderate resistant response and 'Euisung' showed resistant response. To evaluate cultivar resistance of garlics quantitatively disease progress curve was transformed as linear regression equation. Among the intercept (early disease incidence) and the slope (disease infection rate) obtained from the transformed equation, final disease incidence, and area under disease progress curve(AUDPC), AUDPC was the most effective to evaluate disease resistance of garlics to both of white rot pathogens. There was little correlation between mycelial growth and sclerotial formation on garlic cloves in vitro, and epidemiological parameters (early disease incidence, final disease incidence, disease infection rate, AUDPC) obtained from the field trial. Mycelial growth of S. cepivorum was positively correlated with final disease incidence and disease infection rate obtained from the field trial, meanwhile DRSF was negatively correlated with the epidemiological parameters.

• 한국해양학회지
• /
• v.24 no.3
• /
• pp.157-164
• /
• 1989

Quantitative species distribution and primary productivity of phytoplankton were studied monthly from August, 1987 to July, 1988 along with the quantitative distribution of total heterotrophic bacterioplankton and three groups of physiologically chracteristic bacterioplankton in the intertidal and subtidal waters off Kum River Estuary, Yellow Sea. A total of 121 phytoplankton taxa including 102 diatoms occurred, and cell concentration ranged from 15 to 5451 (cells/ml). The great spatio-temporal variations of the number of phytoplankton species and cell concentration well reflected the environmental differences between the intertidal and subtidal waters. Primary productivity (in Piopt, mgC/$m^3$/hr) ranged from 0.6 to 27.3. Just after the phytoplankton bloom (March) Piopt was very low in April at station 1, where amylolytic bacterioplankton also showed quite low population density. The peaks of primary productivity were not always coincided with those of phytoplankton standing crop. The ratio of Piopt's between samples well indicated the environmental differences between the intertidal and subtidal waters. Little characteristic trend was found in the scatter diagrams of phytoplankton standing crop along the population densities of total heterotrophic bacterioplankton and the three groups of physiologically characteristic bacterioplankton. In summer the phytoplankton standing crop was minimum in contrast with the high population density of bacterioplankton, which implies the influx of much allochthonous orgainc matter from Kum River. The scatter diagrams of Piopt along bacterioplankton population density revealed some phenomena there. Piopt had highly positive correlation with the population density of amylolytie bacterioplankton($R^2$=0.84) and that of lipolytic bacterioplankton($R^2$=0.70) while total heterotrophic bacterioplankton and proteolytic bacterioplankton had lesser correlations with Piopt. From the regression lines the increase of unit Piopt (mgC/$m^3$/hr) in the study area was calculated to mean the increase of $9.0{\times}10$ cells/ml and $8.0{\times}10$ cells/ml of amylolytic bacterioplankton and lipolytic bacterioplankton, respectively.