• 한국균학회지
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• 제23권4호통권75호
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• pp.359-368
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• 1995

F. napiforme, F. beomiforme and F. nygamai could not be classified in any of the existing sections of the genus Fusarium. To discuss of the exact taxonomic relationships among these species, the cellular polypeptide patterns were compared by using 2-D PAGE. Polypeptide pattern of F. beomiforme was different from those of other two species and was more similar to F. oxysporum in section Elegans. F. nygamai and F. napiforme might be another same section which would lie between section Liseola and section Elegans. The results were consistent with the comparison of isoenzyme patterns in these species.

• 한국동물학회지
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• 제32권2호
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• pp.75-83
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• 1989

한국 및 일본산 버들치속 어류의 유전적 변이, 종의 분류학적 위치 및 계통변화를 구명하기 위하여 전기영동법을 이용, 26개 유전자를 검출 분석하였다. 버들치속 어류의 평균 유전적 변이 정도는 타 어류군에 비하여 낮은 편이었다. 분류상 문제시 되어 오던 한국산 M. lagowskii와 M. oxycephalus 및 일본산 M. steindachneri와 M. jouyi는 각 종 특유의 genetic markers를 갖고 있어 각기 독립된 별종으로 확인되었다. M. lagowskii의 분포지역인 경포호집단은 유전적으로 뚜렷한 차이가 있고 형태적 특징으로 보아 M. percnurus로 추정된다. 각 종의 분화연대를 추정한 결과 이들은 선신세후기에서 홍종세 초기에 걸처 종분화가 되었고, 고 Amur 하 수계를 통하여 이주, 분포한 것으로 추측된다.

• Journal of the Korean Wood Science and Technology
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• 제15권3호
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• pp.1-13
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• 1987

This study was carried out to investigate the variations of dimension and exterior shape of vessel elements, morphology of spiral thickening and ray-vessel pit in korean diffuse-porous woods (56 species, 18 families), The tangential pore diameter and vessel element length was increased, whereas the pore number per unit area($1mm^2$) was decreased from pith outwards. The tangential pore diameter was decreased but the length of vessel element was not changed from earlywood to latewood within an annual ring. However, the dimensional variations of vessel element was not able to be recognized among relative positions in peripheral variation. The exterior shape of vessel element could be classified into four types; Type 1 is without tail, Type 2 with ligulate tail, Type 3 with broad taper tail and Type 4 with the very short length between perforations. The distribution frequency of Type 2 and 3 was relatively high in comparision with the others. According to the prominence, distribution position and branched shape, the spiral thickenings could be divided into five types. The spiral thickenings occurred 52% in the species observed. Thus it was doubtful to consider the simple presence of spiral thickening as diagnostic index in diffuse-porous woods. The morphology of ray-vessel pit could be grouped as reticulate, scalariform, oval, linear and coalescent type. Most of species examined showed oval and linear type.

• Journal of Plant Biology
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• 제37권1호
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• pp.43-51
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• 1994

고사리삼목, 고사리삼과에 속하는 2속 14종과 이들과의 비교를 위하여 마라티아목(Marattiales)의 Angiopteris 1종 및 고비목의 고비속(Osmunda) 3종 등 18종류의 포자의 극면의 형태를 비교한 결과 고사리삼속(Botrychium) 식물은 둥근 삼각형 또는 변이 오목한 둥근 삼각형으로, 나도고사리삼속(Ophioglossum)과 Angioptris 및 고비속은 원형으로 나타났다. 고사리삼속은 표벽의 미세 무늬에 따라 세 군으로 유집이 가능하였으며 이는 기존의 분류체계와 잘 일치하였다. 표벽의 미세 형태는 고사리삼속에 있어 아속간에 뚜렷한 차이를 보이나 이러한 차이가 나도고사리삼속이나 Angiopteris 혹은 고비속과의 차이보다 적은 것으로 나타나서 고사리삼속내 분류는 Clausen(1938)의 견해를 지지하고 있었다. 나도고사리삼속은 극면의 형태가 구형이며 추문상 혹은 유공상의 표벽무늬를 가져 한 군으로 뚜렷히 구분되었다. 그러나 속내에서는 포자의 특징으로 유집이 불가능하였으며 따라서 Clausen(1938)이나 kato(1987)의 견해를 지지하고 있었다. 아울러 조사된 11종류에 대한 포자 형태의 기재와 검색표를 제시하였다.

• 분석과학
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• 제11권1호
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• pp.54-61
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• 1998

토천궁과 일천궁이 한방이나 가공시 기초 자료로 활용될 수 있도록 기본적인 화학 성분을 분석하였다. 일반성분은 토천궁이 조단백 함량이 높았으며, 무기질은 일천궁이 토천궁에 비해 칼륨과 나트륨이 높은 반면, 그외는 토천궁이 전체척으로 높았다. 유리당 조성은 sucrose만 검출되었고, fructose와 glucose는 검출되지 않았다. 지방산 조성은 linoleic acid, oleic acid, palmitic acid, linolenic acid 순으로 구성되었고, 토천궁과 일천궁의 주요 구성아미노산은 글루탐산, 아르기닌, 아스파르트산, 발린 순으로, 대부분 필수아미노산도 고루 분포되어 있었다.

• Asian-Australasian Journal of Animal Sciences
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• 제18권6호
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• pp.884-891
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• 2005

This paper describes the purification and properties of a multicatalytic proteinase complex, proteasome, from bovine skeletal muscle, in comparision with proteasome prepared from other species or organs. The purified bovine skeletal muscle proteasome exhibited a single band on polyacrylamide gel electrophoresis under nondenaturing conditions. Bovine skeletal muscle proteasome degraded synthetic peptides maximally at pH 8.0. Relative to pH 8.0, activities were gradually decreased with the lowering pH, but the extent of decrease was substrate-dependent, and the activity at pH 5.5 still retained 78-10% of the activity at pH 8.0, indicating the possibility that the proteasome is active in muscle during aging. When the proteasome was heated at 60$^{\circ}C$ for 15 or 30 min and treated in the presence of 0.0125% SDS, the activity increased over 1.8 and 3.1 times (LLVY (Suc-Leu-Leu-Val-Tyr-NH-Mec) as a substrate), respectively. These results (activation with heat or SDS) indicate that the hydrolytic activity of proteasome was stimulated under mild denaturing conditions. The characteristics of the bovine skeletal muscle proteasome obtained in our experiment were almost the same as those of the proteasome prepared from other species or organs.

• 한국양식학회:학술대회논문집
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• 한국양식학회 2005년도 춘계학술발표대회 논문요약집
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• pp.74-75
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• 2005

• 약학회지
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• 제37권2호
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• pp.193-197
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• 1993

For the investigation of medicinal resources in Crataegus species, a study was carried out to clarify the constituents in the leaves of Crataegus Pinnatifida var. pubescens (Rosaceae), of which fruits have been used to stomachic, digestive, astringent, analgesic, and cardiotonic, etc. as a folk medicine in Korea. From the n-BuOH fraction of MeOH extract two flavone glycosides, vitexin(apigenin-8-C-$\beta$-D-glucopyranoside) and 2"-O-rhamnosyl vitexin(apigenin-8-C-$\alpha$-L-rhamnosyl-(1$\rightarrow$2)-$\beta$-D-glucopyrano side) were isolated and identified on the basis of their physico-chemical properties and spectroscopic evidences(UV, IR, NMR, FAB-Mass etc.) in comparision with authentic samples.amples.

• Clinical and Experimental Reproductive Medicine
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• 제19권2호
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• pp.133-141
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• 1992

We examined effects of co-culture with human oviduct epithelial cells (HOEC) on the development of mouse and human embryos from early embryonic· stage to late morula or blastocyst stage (LM or B). In human, embryos were transferred and pregnancy rate was investigated. The HOEC, collected from surgically removed fallopian tube, were cultured in medium-199 supplemented with 20 % fetal cord serum (FCS). The HOEC were characterized by using immunocytochemical staining with anticytokeratin antibody and then used for cultures of mouse and human embryos. Results obtained from co-culture system were as follows. Development rate of mouse embryos was improved by co-culture system at late developmental stage (p<0.025). Human supernumerary embryos remained after transfer, unsuitable for freezing because of their poor quality, were co-cultured for 72hrs. Co-culture (78.79%) or conditioned medium (78.26%) system improved the developmemt rate, significantly, in comparision with control (11.11%)(p<0.00l). Co-cultured (85.71%) human zygotes for 24hrs showed the better development rate in comparision with control (50.00%) (p<0.01). When we transferred embryos cultured with the HOEC to patients, we obtained one pregnancy. Co-cultured human zygotes for 24hrs showed the better quality and viability for the replacement in comparision with control (p<0.01). In addition, improved pregnancy rate was obtained. Our results suggest that the co-culture system can rescue early degenerating embryos by improving early development and yield a resonable number of blastocyst for the appropriate replacement. The effect provided by cultured HOEC is not species specific for the development of embryos and it can be used to overcome in vitro blocks for the development. And also the co-culture system offers the possibility to freeze embryos at blastocyst stage which is more sucessful stage for the freezing. The HOEC monolayer may provide some stimulus via specific factor, which is unknown, to the development of embryos. Our results showed that the co-culture system with HOEC can be an alternative to conventional culture system.

• 한국조경학회지
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• 제22권3호
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• pp.79-90
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• 1994

This survey was ocnducted to investgate changes of the plant community structure from 1972 to 1993 in forest of Mt. Kwanak and thirty-five plots that the size of each plot was 10m${\times}$10m(100㎡) were set up and the vegetation analysis was caried out. By the TWINSPAN analysis, the plant community of survey area were divied into Quercus mongolica, Q. mongolica-Pinus densiflora(1), Q. mongolica-P. densiflora(2), Q. acutissima community. The successional trends of the woody species were seemed to be from P. densiflora, Sorbus alnifolia to Q. mongolica in the canopy layer. But the successional trends in the understory and shrub layer were difficult to suppose. The forest vegetation of Mt. Kwanak from 1972 to 1993 was severely decreased in species number and individuals. The sensitive species for the environmental pollution were selected, and the tolerant plants for the acid soil were increased. In comparision with the DBH class distribution from 1972 to 1993, it shows that the ecological succession has stopped. In the analysis of soil characteristics, soil acidification has taken place over last twenty-two years(from pH=5.40 to pH=4.53). The concentration of K+, Ca++ was severly decreased(from K+=0.60m.e./100g to K+=0.06m.e./100g, from Ca++=3.20m.e./100g to Ca++=0.63m.e./100g), which also could seemed to be cause of plant community decline.