• The Korean Journal of Mycology
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• v.23 no.4 s.75
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• pp.359-368
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• 1995

F. napiforme, F. beomiforme and F. nygamai could not be classified in any of the existing sections of the genus Fusarium. To discuss of the exact taxonomic relationships among these species, the cellular polypeptide patterns were compared by using 2-D PAGE. Polypeptide pattern of F. beomiforme was different from those of other two species and was more similar to F. oxysporum in section Elegans. F. nygamai and F. napiforme might be another same section which would lie between section Liseola and section Elegans. The results were consistent with the comparison of isoenzyme patterns in these species.

• The Korean Journal of Zoology
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• v.32 no.2
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• pp.75-83
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• 1989

Surveys of electrophoretic variation in isozymes and general proteins encoded by 26 loci were conducted to assess species recognition and to estimate the degree of genic variation and species divergence for seven species of the genus Moroco inhabiting in Korea and Japan. Estimates of the average calculated heterozygosity per species of M semotilus, M sp., M percnurus, M lagowskii, M oxycephalus, M steindachneri and M jouyf are low: 0.021, 0.019, 0.051, 0.031, 0.023, 0.046, and 0.007, respectively, and observed heterozygosities are 0.038, 0.022, 0.060, 0.027, 0.025, 0.042, and 0.002, respectively. Allozyme analyses show these species to be distinct genetically with the lafter four species being more closely related one another than any one of them is to the rest of the species. However, these four species (M. lagowskii, M. oxycephalus, M. steindachneri and M jouyi), had unique genetic markers in each species to be recognized as valid species. These results contrast to the previous report of Chung et of. (1986) mainly due to their error in analyzing the isozyme pallems, particularly in MDH and PGI analyses. The genetic distances among M semotilus, M sp., and M percnurus are near the high end of the scale of such estimate for freshwater fish congeners. Based on estimated divergent time of these species of the genus Moroco (5 to 0.6 million years) it is assumed that they are speciated during late Pliocene to middle Pleistocene epoch prior to migration to Korean and Japanese waters through Paleo Amur River system.

• Journal of the Korean Wood Science and Technology
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• v.15 no.3
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• pp.1-13
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• 1987

This study was carried out to investigate the variations of dimension and exterior shape of vessel elements, morphology of spiral thickening and ray-vessel pit in korean diffuse-porous woods (56 species, 18 families), The tangential pore diameter and vessel element length was increased, whereas the pore number per unit area($1mm^2$) was decreased from pith outwards. The tangential pore diameter was decreased but the length of vessel element was not changed from earlywood to latewood within an annual ring. However, the dimensional variations of vessel element was not able to be recognized among relative positions in peripheral variation. The exterior shape of vessel element could be classified into four types; Type 1 is without tail, Type 2 with ligulate tail, Type 3 with broad taper tail and Type 4 with the very short length between perforations. The distribution frequency of Type 2 and 3 was relatively high in comparision with the others. According to the prominence, distribution position and branched shape, the spiral thickenings could be divided into five types. The spiral thickenings occurred 52% in the species observed. Thus it was doubtful to consider the simple presence of spiral thickening as diagnostic index in diffuse-porous woods. The morphology of ray-vessel pit could be grouped as reticulate, scalariform, oval, linear and coalescent type. Most of species examined showed oval and linear type.

• Journal of Plant Biology
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• v.37 no.1
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• pp.43-51
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• 1994

Spore morphology of 14 species of Ophioglossaceae is examined, and descriptions and key based on spore morphology are provided. In addition, spores of one species of Angiopteris and three species of Osmunda are examined for comparision with those of Ophioglossaceae. Spore shape at proximal face is sub triangular or triquete in Botrychium and circular in Ophiog~ lossum, Angiopteris and Osmunda. Based on the wall sculpturing patterns, three groups can be recognized within Botrychium, and the differences of wall sculpturing among these groups are smaller as compared to those among Botrychium, Ophioglossum, Osmunda and Angiopteris. This result supports the previous infrafamilial system of Clausen, which was mainly based on the characteristics of vernation, habit, fertile and sterile segments of the leaf, and gametophyte. Spores of nine species of Ophioglossum, which are circular in proximal face and fossulate or foveolate in wall sculpturing, are quite uniform as compared to those of Botrychium. Therefore they should be kept under a single genus rather than split into four genera suggested by Nishida (1952).(1952).

• Analytical Science and Technology
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• v.11 no.1
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• pp.54-61
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• 1998

Chemical components of domestic Ligusticum chuanxiong HORT and Cnidium officinale MAKINO were analyzed. Proximate analysis of each species showed crude protein 18.5% and 11.1%, crude lipid 5.9% and 6.2%, crude fiber 6.4% and 6.5%, crude ash 5.4% and 6.0%, and carbohydrate 63.7% and 70.2%, respectively. Contents of potassium which was found to be the most abundant mineral in both species were 1.8% and 3.0%, and those of sucrose were 0.4% and 0.3% respectively while neither fructose nor glucose were detected in each species. Major fatty acids in Ligusticum chuanxiong HORT and Cnidium officinale MAKINO were linoleic acid (60.7% and 61.2%), oleic acid (19.9% and 21.3%), palmitic acid (11.4% and 11.3%)(respectively) but there was no significant difference between two species. Glutamic acid was revealed as the most abandant amino acid in both species with 2.5% in Ligusticum chuanxiong HORT and 1.6% in Cnidium officinale MAKINO. Ligusticum chuanxiong HORT and Cnidium officinale MAKINO also were shown to be contained 0.0009% and 0.0005% vitamin $B_1$, and 0.6% and 0.7% tannin, respectively.

• Asian-Australasian Journal of Animal Sciences
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• v.18 no.6
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• pp.884-891
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• 2005

This paper describes the purification and properties of a multicatalytic proteinase complex, proteasome, from bovine skeletal muscle, in comparision with proteasome prepared from other species or organs. The purified bovine skeletal muscle proteasome exhibited a single band on polyacrylamide gel electrophoresis under nondenaturing conditions. Bovine skeletal muscle proteasome degraded synthetic peptides maximally at pH 8.0. Relative to pH 8.0, activities were gradually decreased with the lowering pH, but the extent of decrease was substrate-dependent, and the activity at pH 5.5 still retained 78-10% of the activity at pH 8.0, indicating the possibility that the proteasome is active in muscle during aging. When the proteasome was heated at 60$^{\circ}C$ for 15 or 30 min and treated in the presence of 0.0125% SDS, the activity increased over 1.8 and 3.1 times (LLVY (Suc-Leu-Leu-Val-Tyr-NH-Mec) as a substrate), respectively. These results (activation with heat or SDS) indicate that the hydrolytic activity of proteasome was stimulated under mild denaturing conditions. The characteristics of the bovine skeletal muscle proteasome obtained in our experiment were almost the same as those of the proteasome prepared from other species or organs.

• Proceedings of the Korean Aquaculture Society Conference
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• 2005.05a
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• pp.74-75
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• 2005

• YAKHAK HOEJI
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• v.37 no.2
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• pp.193-197
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• 1993

For the investigation of medicinal resources in Crataegus species, a study was carried out to clarify the constituents in the leaves of Crataegus Pinnatifida var. pubescens (Rosaceae), of which fruits have been used to stomachic, digestive, astringent, analgesic, and cardiotonic, etc. as a folk medicine in Korea. From the n-BuOH fraction of MeOH extract two flavone glycosides, vitexin(apigenin-8-C-$\beta$-D-glucopyranoside) and 2"-O-rhamnosyl vitexin(apigenin-8-C-$\alpha$-L-rhamnosyl-(1$\rightarrow$2)-$\beta$-D-glucopyrano side) were isolated and identified on the basis of their physico-chemical properties and spectroscopic evidences(UV, IR, NMR, FAB-Mass etc.) in comparision with authentic samples.amples.

• Clinical and Experimental Reproductive Medicine
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• v.19 no.2
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• pp.133-141
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• 1992

We examined effects of co-culture with human oviduct epithelial cells (HOEC) on the development of mouse and human embryos from early embryonic· stage to late morula or blastocyst stage (LM or B). In human, embryos were transferred and pregnancy rate was investigated. The HOEC, collected from surgically removed fallopian tube, were cultured in medium-199 supplemented with 20 % fetal cord serum (FCS). The HOEC were characterized by using immunocytochemical staining with anticytokeratin antibody and then used for cultures of mouse and human embryos. Results obtained from co-culture system were as follows. Development rate of mouse embryos was improved by co-culture system at late developmental stage (p<0.025). Human supernumerary embryos remained after transfer, unsuitable for freezing because of their poor quality, were co-cultured for 72hrs. Co-culture (78.79%) or conditioned medium (78.26%) system improved the developmemt rate, significantly, in comparision with control (11.11%)(p<0.00l). Co-cultured (85.71%) human zygotes for 24hrs showed the better development rate in comparision with control (50.00%) (p<0.01). When we transferred embryos cultured with the HOEC to patients, we obtained one pregnancy. Co-cultured human zygotes for 24hrs showed the better quality and viability for the replacement in comparision with control (p<0.01). In addition, improved pregnancy rate was obtained. Our results suggest that the co-culture system can rescue early degenerating embryos by improving early development and yield a resonable number of blastocyst for the appropriate replacement. The effect provided by cultured HOEC is not species specific for the development of embryos and it can be used to overcome in vitro blocks for the development. And also the co-culture system offers the possibility to freeze embryos at blastocyst stage which is more sucessful stage for the freezing. The HOEC monolayer may provide some stimulus via specific factor, which is unknown, to the development of embryos. Our results showed that the co-culture system with HOEC can be an alternative to conventional culture system.

• Journal of the Korean Institute of Landscape Architecture
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• v.22 no.3
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• pp.79-90
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• 1994

This survey was ocnducted to investgate changes of the plant community structure from 1972 to 1993 in forest of Mt. Kwanak and thirty-five plots that the size of each plot was 10m${\times}$10m(100㎡) were set up and the vegetation analysis was caried out. By the TWINSPAN analysis, the plant community of survey area were divied into Quercus mongolica, Q. mongolica-Pinus densiflora(1), Q. mongolica-P. densiflora(2), Q. acutissima community. The successional trends of the woody species were seemed to be from P. densiflora, Sorbus alnifolia to Q. mongolica in the canopy layer. But the successional trends in the understory and shrub layer were difficult to suppose. The forest vegetation of Mt. Kwanak from 1972 to 1993 was severely decreased in species number and individuals. The sensitive species for the environmental pollution were selected, and the tolerant plants for the acid soil were increased. In comparision with the DBH class distribution from 1972 to 1993, it shows that the ecological succession has stopped. In the analysis of soil characteristics, soil acidification has taken place over last twenty-two years(from pH=5.40 to pH=4.53). The concentration of K+, Ca++ was severly decreased(from K+=0.60m.e./100g to K+=0.06m.e./100g, from Ca++=3.20m.e./100g to Ca++=0.63m.e./100g), which also could seemed to be cause of plant community decline.