• Journal of the Korean Wood Science and Technology
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• 제13권3호
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• pp.3-26
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• 1985

The object of this study was to investigate the effects on physical and mechanical properties of wood particle and sawdust board combined with wire net. Conventional forming, press-lam, and veneer comply boards combining one to four wire net sheets were made from wood particle and sawdust with different spacings (8, 10, 12, and 18 Mok) and different wire diameters (0.35, 0.50, and 0.80mm) composing wire net. They were compared and analyzed statistically with specific gravity, thickness swelling, length swelling, bending properties (modulus of rupture, modulus of elasticity, work to proportional limit, and total work), internal bonding strength, and screw holding strength between wood particle and sawdust boards. The results obtained at this study as cording to the discussions might be concluded as follows; 1. In specific gravity, both particle and sawdust boards by press-lam method were higher than by conventional forming and veneer comply method, and the boards containing more wire net sheets also showed higher value. But the wire net spacings(Mok) had no influence on specific gravity. In general, particle board showed higher specific gravity than sawdust board. Veneer comply board showed lowest specific gravity values. 2. Both particle and sawdust boards by press-lam method was slightly lower than by conventional forming and veneer comply method in thickness swelling. The sawdust board containing 8, 12. and 18 Mok wire net showed lower thickness swelling than the corresponding particle board, but both sawdust and particle boards containing the T8 and 10 Mok wire net showed higher and similar thickness swelling. 3. Both particle and sawdust boards containing wire net showed no difference in MOR and MOE of bending. Comply board was the highest and particle board showed slightly higher than sawdust board in MOR and MOE values. 4. In work to proportional limit and total work in bending, both particle and sawdust boards containing thicker wire diameter and more wire net sheets showed higher value. From these facts, it is conceivable that boards with thicker wire diameter and more wire net sheets show increasing resistance against external force. But there was no significant difference between particle and sawdust borads. 5. In resistance against delamination (internal bonding strength), both sawdust and particle boards containing wire net showed lower value than control, and also showed decreasing tendency with more number of wire net sheet composed. Particle board showed higher resistance against delamination than sawdust board. 6. In screw holding strength, sawdust board containing thicker wire diameter and more wire net sheets showed higher value, but particle board by press-lam method was higher than by conventional forming and veneer comply method. Screw holding strength of particle board was higher than that of sawdust board.

• 한국양식학회지
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• 제15권1호
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• pp.61-68
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• 2002

사육기간 중 수온은 10.4∼25.5$^{circ}C$로 해역별, 표$.$저층간의 차이는 크지 않았으며, 염분은 25.00∼31.17 $textperthousand$이었고, 용존산소는 최저 6.13 mg/L 이상으로 양호한 상태를 보였다. Chlorophyll-a는 1.23∼11.05 $\mu\textrm{g}$/L로 저층시험구는 월별 변화가 심하고, 2.00 $\mu\textrm{g}$/L 이하의 저농도를 보였다. 6∼8월 해만가리비 성장기에 Phytoplankton 우점종은 편모조류인 Ceratium sp.가 35.2% 출현하였다. 플랑크톤의 밀도는 대체적으로 8∼9월에 낮았고, 10월에 높았다. 수심에 따른 성장을 보면, 해만가리비의 치패 (평균각고 16.59∼16.69 mm)를 2 m와 5 m 수출에서 사육한 결과, 각고는 표층시험구가 0.19 mm$.$d$^{-1}$이었고, 저층시험구는 0.16 mm$.$d$^{-1}$이었다. 전중량은 표층시험구가 0.16 g$.$d$^{-1}$ 이었고, 저층시험구는 0.12 g$.$d$^{-1}$ 이었다. 각고의 일간성장률은 표층시험구는 0.606%이었고, 저층시험구는 0.549%이었다. 전중량의 일간성장률은 표층시험구가 1.972%이었고, 저층시험구는 1.781%이었다. von Bertalanffy 성장 모델에 의해 얻어진 각고의 최대 예상값은 52.62 mm (표층시험구) 및 46.73 mm (저층시험구)이었다. 생존율은 표층구가 80.0%로 저층구보다 높아 표층 (수심 2 m)이 저층 (수심 5 m)보다 해만가리비의 성장 및 생존에 적합한 수층이었다.

• 한국미생물·생명공학회지
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• 제7권3호
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• pp.135-140
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• 1979

특이하게도 일단 어떤 원인으로든지 변성이 일어나서 침전이 형성되고 난 후에는 그 변성으로 인하여 다시 용해되지 않는 penicillinase를 생성분비하는 Streptomyces 속 균주 AS-727을 토양으로부터 얻었으며, 이 균의 효소생산성과 이 효소의 일반적성질을 검토한 결과는 다음과 같다. 1) 본 효소의 생산을 위해서는 질소원으로서 sodium nitrate, 탄소원으로서 glucose 나 maltose를 사용하여 4 일간 배양하는 것이 바람직했으며 2) 정제는ammonium sulfate로 완전포화시켜 생성된 침전을 원심분리로 모아 그것을 투석해서 조정제효소를 얻는데, 이때 투석할 때 침전은 용해 되지 않는다. 3) 본 효소의 최적 pH는 중성부근 (6.0~8.0)이며 최적온도는 4$0^{\circ}C$ 근처이고, 4) 안정 pH 범위는 4.0~9.0으로서 비교적 넓었으며 열에 대해서는 4$0^{\circ}C$에서는 안정했으며 6$0^{\circ}C$와 8$0^{\circ}C$에서 60 분간 처리하면 각각 약 30%와 40%가 실활되었다. 5) 본 효소의 활성에 있어서 zinc chloride가 $10^{-3}$ M 농도에서 약 20%, $10^{-2}$ M 이상의 고농도에서는 95% 이상을 조해하였다. 6) 그리고 동결이나 명종 고류 또는 유기용매로 일단 형성된 효소단백침전은 다시 용해되지 않는 특이한 성질을 가졌다.

• 대한의생명과학회지
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• 제12권1호
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• pp.29-33
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• 2006

The uptake of cadmium in animals is mainly accumulated in and affected to the liver and kidney by binding with red blood cells and serum albumin. The process accounts for more than 50% of the total accumulated cadmium in the body. The kidneys may be damaged without regarding the pathway uptake of cadmium. In a group of rats on supplements of 1% chlorella and 40 ppm cadmium, the concentration of cadmium in urine greatly decreased by 66% compared to control group, and the total synthesis of metallothionein decreased by 48.6% compared to control group. However, no previous study has assessed the protective effect on kidney damage induced by cadmium uptake through supplementation with chlorella. This study analyzed the biochemical marker for kidney damage in the rats after uptake of 40 ppm $CdCl_2$ and supplementation of the diet of Sprague Dawley (SD) rats with 1%, 5%, and 10% chlorella during 4 weeks. In a group of SD rats on supplementation with 1% chlorella and uptake of 40 ppm $CdCl_2,\;\beta_2$ microglobulin in the urine was found to be $3.1\pm0.6\;{\mu}g/L$, a decrease of 58% compared to a group of Sp rats on uptake of $CdCl_2$ only, in which the $\beta_2$ microglobulin was found to be $4.9\pm0.7\;{\mu}g/L$. According to the results of histopathological observation, the accumulation of mild and localized chronic inflammatory cells in kidney tissues was observed in 50% of the SD rats on uptake of cadmium only. In contrast, only 30% of the SD rats on supplementation with 1% chlorella and uptake of 40ppm $CdCl_2$, representing a histopathological abnormality, and there were no histopathological abnormalities at all in groups of SD rats on supplementation with 5% or 10% chlorella and uptake of 40 ppm $CdCl_2$. In conclusion, protein, calcium, and iron, which account for more than 50% of the total dried chlorella composition, may contribute to the reduction nephrotoxicity by stimulating both inhibited absorption of cadium and increased excretion of accumulated cadmium in kidneys.

• 한국발생생물학회지:발생과생식
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• 제15권2호
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• pp.121-131
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• 2011

Amphiprion melanopus의 난발생과 자치어의 형태학적 발달에 대한 기초 조사와 함께, 먹이생물과 갑상선호르몬이 자치어의 성장과 체색에 미치는 영향을 조사하였다. 수정 후, 부화까지 총 168시간이 소요되었다. 갓 부화 자어의 크기는 평균 $3.5{\pm}0.3$ mm 으며, 대부분의 자어는 부화 후 경과일 수에 따라 체색 변화(변태)를 나타내었다; DAH(days afterhatching) 10: 체색 흑화, DAH 15~20: 3개의 흰 줄무늬 출현, DAH 30: 등과 꼬리지느러미의 흰 줄무늬가 사라지기 시작,DAH 90: 체색 주황색; 성어: 체색은 진한 계피색이며 머리에만 흰 줄무늬 남음. Artemia와 T. japonicus를 함께 공급한실험구의 성장은 Artemia만 공급한 실험구보다 빨랐다. T. japonicus 만을 공급한 실험구의 지느러미 체색은 진하고 선명한 주황색이었다. 갑상선호르몬을 처리한 4 ppm구의 성장이 다른 실험구(0, 2, 6 ppm)보다 빨랐고 6 ppm구가 가장 느렸다. 본 연구의 결과, 주요 먹이생물로 알려져 있는 Artemia와 함께 T. japonicus를 공급함으로써 A. melanopus의 성장과체색을 개선시킬 수 있었고, 갑상선호르몬 또한 변태 및 체색 형성에 직접적으로 관여할 것으로 사료된다.

• Journal of Applied Biological Chemistry
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• 제51권6호
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• pp.272-276
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• 2008

정제효소에 의해 locust bean gum galactomann을 가수분해하여 activated carbon column chromatography에 의해 당가수분해물을 분리 회수하여 TLC에 의해 주요 당가수분해물은 중합도 4와 6의 hetero type으로 확인되었으며 D.P. 4의 구조식은 비환원말단 mannose로 부터 2번째에 1분자의 galactose가 결합하고 있는 hetero type의 구조인 ${Gal^2}{Man_3}(6^2-mono-O-{\alpha}-D-galactopyranosyl-4-O-{\beta}-D-mannotriose)$로, D.P. 6의 구조식은 비환원말단 mannose로부터 4번째에 1분자의 galactose가 결합하고 있는 hetero type의 구조인 ${Gal^2}{Man_5}(6^2-mono-O-{\alpha}-D-galacto-pyranosyl-4-O-{\beta}-D-mannopentaose)$로 유추하고 있다. B. longum, B. bifidum, B. adolescentis, B. animalis, B. auglutum, B. breve의 생육활성에 대한 중합도 4와 6의 영향을 검토하기 위하여 modified-MRS배지 상에 탄소원으로 중합도 4와 6를 대체하여 생육활성을 비교한 결과 B. longum에서는 D.P 6 galactomannooligosaccharide를 탄소원으로 대체한 경우 표준 MRS배지와 비교하여 10배의, D.P. 4을 처리한 경우에도 7.6배의 상대 활성을 나타내어 가장 우수한 생육활성을 나타냈었으며, B. bifidum의 경우에서도 D.P 6에서 6.6배, D.P 4에서 4.8배의 활성을 나타내었으며 B. animalis에 있어서는 D.P. 6의 경우 3.8배의 상대활성을 나타내었다. Bifidobacterium 7균주 모두에 대해서 중합도 6의 올리고당이 중합도 4의 올리고당보다 생육활성에 크게 기여하는 것으로 나타났다. 신규 MRS 배지의 제품화와 관련하여 가장 유리한 탄소원 대체소재는 Bacillus sp. 유래 galactosyl mannooligosaccharide 인 ${Gal^3}{M_4}$가 가장 유리한 소재로 결정되어 신규 배지의 scale-up 공정을 구축중에 있다.

• Asian-Australasian Journal of Animal Sciences
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• 제23권9호
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• pp.1184-1189
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• 2010

This work was conducted to examine the variation of immunoglobulins (Igs) in serum, immune milk, normal milk and colostrum upon implantation of a new Antigen Releasing Device (ARD). The core of each ARD housed an immunostimulating complex (ISCOM) that was made of adjuvant Quil A and type XIII lipase from a Pseudomonas sp. Each ARD was coated with polylactic acid, known as polylactide, that controls antigen release. Twenty lactating Chinese Holstein cows were divided into 2 groups (n = 10): test group and control group. All cows in the test group were implanted with a single injection in the right iliac lymph node with 3 types of ARDs, which were designed to release the antigens at d 0, 14 and 28 post-implantation. Blood and milk samples were collected from both groups, and colostrum samples were also collected from other post-partum cows in the same farm. Concentrations of $IgG_1$, IgA and IgM in whey and serum were measured by sandwich ELISA. The results showed that the $IgG_1$, IgA and IgM concentrations in serum and whey from the test group were higher than from the control group. Among the three Igs measured, the $IgG_1$ concentration in serum was significantly higher at d 40 after ARD implantation, and the $IgG_1$ concentration in whey peaked at d 9, 17 and 30, which corresponded with release of the antigen. Based on Pearson's correlation between Ig concentration and production parameters, IgA concentration in normal milk was positively correlated with lactation period, which reflected IgA changes during the lactation period in immune milk. In colostrum, $IgG_1$, IgA and IgM decreased abruptly from d 0 to 3, and then decreased slightly. In conclusion, serum $IgG_1$ concentration can be affected by controlled release of the ARD, while whey IgA levels are primarily affected by lactation period. These results may be useful in future studies designed to regulate concentrations of Igs in immune milk.

• Journal of Biosystems Engineering
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• 제33권5호
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• pp.362-369
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• 2008

There is developing interest in precision agriculture in Korea, despite the fact that typical Korean fields are less than 1 ha in size. Describing within-field variability in typical Korean production settings is a fundamental first step toward determining the size of management zones and the inter-relationships between limiting factors, for establishment of site-specific management strategies. Measurements of rice (Oriza Sativa L) yield, chlorophyll content, and soil properties were obtained in a small (100-m by 30-m) Korean rice paddy field. Yield data were manually collected on 10-m by 5-m grids (180 samples with 3 samples in each of 60 grid cells) and chlorophyll content was measured using a Minolta SPAD 502 in 2-m by 2-m grids. Soil samples were collected at 275 points to compare results from sampling at different scales. Ten soil properties important for rice production in Korea were determined through laboratory analyses. Variogram analysis and point kriging with and without median polishing were conducted to determine the variability of the measured parameters. Influence of variogram model selection and other parameters on the interpretation of the data was investigated. For many of the data, maximum values were greater than double the minimum values, indicating considerable spatial variability in the small paddy field, and large-scale spatial trends were present. When variograms were fit to the original data, the limits of spatial dependency for rice yield and SP AD reading were 11.5 m and 6.5 m, respectively, and after detrending the limits were reduced to 7.4 m and 3.9 m. The range of spatial dependency for soil properties was variable, with several having ranges as short as 2 m and others having ranges greater than 30 m. Kriged maps of the variables clearly showed the presence of both large-scale (trend) variability and small-scale variability in this small field where it would be reasonable to expect uniformity. These findings indicate the potential for applying the principles and technology of precision agriculture for Korean paddy fields. Additional research is needed to confirm the results with data from other fields and crops.d similar tendency with the result for the frequency less than 20 Hz, but the width of change was reduced highly.

• Asian Pacific Journal of Cancer Prevention
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• 제14권12호
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• pp.7179-7186
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• 2013

Background and Aims: Scutellaria is one of the most popular traditional Chinese herbal remedies against various human diseases, including cancer. In this study, we examined the active effects of Scutellaria extract and its main flavonoid constituents on the proportion of side population cells within human multiple myeloma cell line RPMI8226 in vitro and explored the potential molecular mechanisms involved. Materials and Methods: The contents of flavonoids in ethanolic extract of Scutellaria baicalensis Georgi were determined using high performance liquid chromatography. The antiproliferative effect of the ethanolic extract on RPMI-8226 was determined by CCK assay. Apoptosis was measured by annexin combining with propidium iodide in a flow cytometer. Cell cycle analysis was performed by propidium iodide staining in combination with flow cytometry analysis. Hoechst 33342 exclusion assay was used for the identification of side population within RPMI8226 cells. The expression of ABCG2 protein was assessed by Western blotting assay. Results: The content of major flavonoids constitutents of Scutellaria extract was baicalin (10.2%), wogonoside (2.50%), baicalein (2.29%), and wogonin (0.99%), respectively. The crude Scutellaria extract did not show significant anti-proliferative effect, apoptosis induction and cell cycle arrest in RPMI-8226 within the concentrations of $1-75{\mu}g/mL$. However, the ethanolic extract, baicalein, wogonin and baicalin reduced the side population cells in RPMI-8226, and data showed that baicalein and wogonin had stronger inhibitory effects. Correspondingly, they also exhibited significant effects on decreasing the expression level of ABCG2 protein in RPMI-8226 in vitro. Conclusions: Our results for the first time demonstrated a novel mechanism of action for Scutellaria extract and its main active flavonoids, namely targeting SP cells by modulating the expression of ABCG2 protein. This study provides an insight for new therapeutic strategies targeting cancer stem cells of multiple myeloma.

• The Korean Journal of Physiology and Pharmacology
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• 제10권4호
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• pp.217-222
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• 2006

Atherosclerosis is considered as a chronic inflammatory process. However, the nature of the oxidant signaling that regulates monocyte adhesion and its underlying mechanism is poorly understood. We investigated the role of reactive oxygen species on the vascular cell adhesion molecule-1 (VCAM-1) and monocyte adhesion in the cultured endothelial cells. $TNF-{\alpha}$ at a range of $1{\sim}30\;ng/ml$ induced VCAM-1 expression dose-dependently. BCECF-AM-labeled U937 cells firmly adhered on the surface of endothelial cells when the endothelial cells were incubated with $TNF-{\alpha}$ (15 ng/ml). Ten $\;{\mu}mol/L$ of SB203580, an inhibitor of p38 MAPK, significantly reduced $TNF-{\alpha}-induced$ VCAM-1 expression, compared to the JNK inhibitor ($40\;{\mu}mol/L$ of SP60015) or ERK inhibitor ($40\;{\mu}mol/L$ of U0126). Also, SB203580 significantly inhibited $TNF-{\alpha}-induced$ monocyte adhesion in HUVEC. Superoxide production was minimal in the basal condition, however, treatment of $TNF-{\alpha}$ induced superoxide production in the dihydroethidineloaded endothelial cells. Diphenyleneiodonium (DPI, $10\;{\mu}mol/L$), an inhibitor of NADPH oxidase, and rotenone $(1\;{\mu}mol/L)$, an inhibitor of mitochondrial complex I inhibited $TNF-{\alpha}-induced$ superoxide production, VCAM-1 expression and monocyte adhesion in the endothelial cells. Taken together, our data suggest that NADPH oxidase and mitochondrial ROS were involved in $TNF-{\alpha}-induced$ VCAM-1 and monocyte adhesion in the endothelial cells.