• 한국식품영양과학회지
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• 제17권3호
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• pp.185-190
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• 1988

아세톤으로 탈지한 대두분으로부터 gel여과법과 이온 교환 크로마토그래피등의 전통적인 단백질 추출 방법을 이용하여 Lipoxygenase 이성효소를 분리하였다. 이온 교환 후의 lipoxygenase-1과 -2는 Crude extract에 비해 각각 19배와 32배 정제 되어졌으나, 상당한 효소활성의 손실을 초래하였으며, lipoxygenase 에 특이적인 염색기술을 이용하여 행한 7% PAGE에서 lipoxygenase-1, -2, 그리고 -3은 각각 0.38, 0.29그리고 0.33의 Rf치를 나타내었다.

• 한국작물학회지
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• 제14권
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• pp.91-96
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• 1973

우리나라 대두의 단위면적당 생산량은 제외국에 비해 낮은 편이며 그 원인은 여러 가지로 생각할 수 있으나 단시일내에 기존품종으로서 생산량을 높이는데 대두 초형에 따르는 재식방법의 개선이 중요하다고 여겨져 중부지방에서의 밀식적응대두형과 밀식정도의 구명을 위해 본 시험이 수행되었다. 본 시험에서는 모형과 각종 형질이 서로 상이한 품종인 장단백목, 충북백, Clark 등 3개 품종이 공시되었으며 휴폭을 세구로 그리고 주간거리를 세세구로 한 3반복으로서 시험하였다. 주당 분지수와 협수는 밀식에 따라 감소되었으나 동일한 밀도에서는 Clark에서 보다 충북백과 장단백목에서 더 많은 분지수를 가지고 있었으며, 협수에 관해서는 휴폭과 주간거리에 유의한 수준의 상호작용이 있었다. 절수는 품종간에 차이가 현저하였으며 휴폭에 의해서는 전혀 변화가 없었으며 주간거리가 커짐에 따라 Clark에서 약간 증가하였다. 간장도 주간거리에 관해서만 유의차가 현저하였으나 절수의 증가와는 관계없이 도장에 기인하였다. 도복성에는 Clark가 가장 강했으며 이는 이 품종 주경의 탄력성 때문인 것으로 여겨졌다. 밀식조건하에서는 다지성이고 광엽성인 재래품종들보다 절수가 많고 초장이 큰 도입품종 Clark가 공시된 3개 품종중 가장 수량이 많았으며 밀식재배용으로의 가장 알맞은 초형임이 명확하였고 파종은 재래식 점파보다는 조파로 밀식함이 노력이 절약되고 단위면적당 수량도 현저히 증가될 것이다.

• 한국작물학회지
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• 제48권
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• pp.58-64
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• 2003

To enjoy a healthy life, it is important to have a well-balanced diet. However, in today's society, there is an increase in the consumption of preprocessed foods and frequency of eating out. Also the western diet, which is becoming move popular worldwide, contains relatively high levels of protein and fat, and a low amount of fiber, Furthermore, the increased availability of favorite foods has created a condition were the individual diet is less variable. With these conditions, it is difficult to maintain a diet that is nutritionally balanced. With these unbalanced diets, which are difficult to change, there has been an increase in adult disease and health problems, such as colon and breast cancer, It is speculated that metabolites for carcinogens are produced from diet components and that intestinal bacteria contribute to the production of these metabolites. Therefore, it is necessary to evaluate the relationships between health, diet, and intestinal microflora. Soybean oligosaccharide is composed of water-soluble saccharides that have been extracted from soybean whey, a by-product from the production of soy protein. This is mainly a mixture of mono-, di-, tri-, and tetrasac-charides, with the principle components being the oligosaccharide raffinose and stachyose. When consumed by humans, the oligosaccharides cannot be digested in the human duodenal and small intestinal mucosa, and these are selectively utilized by beneficial bifidobacteria in intestines. The results of acute and subacute toxicity tests, soy-bean oligosaccharides were nonpoisonous. Soybean oligosaccharides promote the growth of indigenous bifido-bacteria in the colon which by their antagonistic effects, suppress the activity of putrefactive bacteria. Also, they reduce toxic metabolites, detrimental enzymes and plasma lipid, and increase in the frequency of bowel evacuation and fecal quantities. Consequently, soybean oligosaccharides as functional foods components have potential roles in the prevention and medical treatment of chronic adult diseases. The study of processing property and physiological function of soybean oligosacchavides and development of high oligosaccharide variety allow the creation of new and exciting foodstuffs that aye functional healthy.

• 한국작물학회지
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• 제41권2호
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• pp.168-172
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• 1996

콩품종 타치나가하(Tachinagaha)를 공시하여 토양수분조건에 따른 식물체내의 수분상태 즉 잎의 수분 potential, 광합성 및 생육에 미치는 영향을 검토하고자 실험을 수행한 결과를 요약하면 다음과 같다. 1. 광량자밀도가 1,000$\mu$mol m$^{-2}$ s$^{-1}$ 이하로 낮아질 때 광합성 속도는 점차 저하하였고, 토양수분 조건에 따른 광합성 속도는 건조구와 과습구는 적습구보다 떨어졌다. 2. 생육시기에 관계없이 광합성 속도와 기공 conductance와는 정의 상관 관계가 인정되었다. 3. 광합성 속도와 기공 conductance는 토양수분 부족에 매우 민감하게 반응하였다. 4. 잎의 수분 potential은 적습구가 과습구 또는 건조구보다 높았으며, 광합성 속도와도 밀접한 관련이 있었다. 5. 잎의 신장은 토양수분에 민감한 반응을 보여 건조구에서 엽면적이 가장 낮았으나 엽록소 함량은 높았다. 6.식물체의 총 건물중은 적습구 > 과습구 > 건조구 순이었으나 건물중에 대한 뿌리의 비율은 건조구에서 높게 나타났다

• The Plant Pathology Journal
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• 제36권6호
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• pp.591-599
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• 2020

Phytophthora root and stem rot reduce soybean yields worldwide. The use of R-gene type resistance is currently crucial for protecting soybean production. The present study aimed to identify the genomic location of a gene conferring resistance to Phytophthora sojae isolate 2457 in the recombinant inbred line population developed by a cross of Daepung × Daewon. Singlemarker analysis identified 20 single nucleotide polymorphisms associated with resistance to the P. sojae isolate 2457, which explained ~67% of phenotypic variance. Daewon contributed a resistance allele for the locus. This region is a well-known location for Rps1 and Rps7. The present study is the first, however, to identify an Rps gene locus from a major soybean variety cultivated in South Korea. Linkage analysis also identified a 573 kb region on chromosome 3 with high significance (logarithm of odds = 13.7). This genomic region was not further narrowed down due to lack of recombinants within the interval. Based on the latest soybean genome, ten leucine-rich repeat coding genes and four serine/ threonine protein kinase-coding genes are annotated in this region, which all are well-known types of genes for conferring disease resistance in crops. These genes would be candidates for molecular characterization of the resistance in further studies. The identified R-gene locus would be useful in developing P. sojae resistant varieties in the future. The results of the present study provide foundational knowledge for researchers who are interested in soybean-P. sojae interaction.

• 한국식생활문화학회지
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• 제14권2호
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• pp.103-113
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• 1999

Jeupjang like salted soybean paste with vegetable is the Korean traditional side order eating at table. Bibliographical studies on the Jeupjang in historic books such as Jeungbo-Sanlim-Keongjae(Re-edition of agriculture economic), Imwon- Keongjae Ji(Book of country economic) and Keuhap-Chongseo(Handbook of household) described the Korean food in the 18 century carried out. In addition, investigation and analytical studies on various home-made Jeupjang in present was accomplished. Jeupjangs were classified into three types according to variety preparing methods. Three types were fermented soybean paste (Doenjang) type using traditional Meju(soybean cake stater) for Jeupjang, salted pickle (Jangachi) type buried cucumber and eggplant into soybean paste or soysauce (Kanjang), and salted sauer kraut (Kimchii) type prepared vegetable in Jeupjang-Meju mash. The procedures for producing Jeupjang were Jeupjang-Meju making, and mixing vegetable with Meju powder into brine. At last process was fermented in horse wastes or grasses for 7-14 days. But manufacturing methods of Jeupjang before the 18 century were different in present. Nowadays glutinous rice, red pepper powder and various vegetable were used for domestic Jeupjang.

• Nutrition Research and Practice
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• 제1권3호
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• pp.206-211
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• 2007

This study estimated the isoflavone intake level in Koreans using Food Frequency Questionnaire and analyzed related variables. The results showed that the average daily intake of isoflavone in adults was shown as 23.1 mg. The isoflavone intake level at 50 percentile was 16.9 mg ($0{\sim}90\;mg$), and 10% of adults took almost 50 mg of isoflavone a day and 10% took about 5 mg a day. The major food sources for isoflavone in Koreans were in the order of soybean, soybean paste, soy milk, soybean curd (tofu), and bean sprouts; the intake was different depending on age, educational background, occupation, economic standard, and family type. The result showed higher isoflavone intake levels in the group over 30 years old and the highest isoflavone intake in subjects working in farming/fishery, followed by housemakers. According to the differences by families the families with elderly members showed 50% higher isoflavone intake than young families with friends or siblings. Depending on related ecological variables, therefore, various nutrition education programs should be developed for a variety of intakes of soybean foods, along with easy and simple cooking methods as parts of continuous research.

• 한국환경성돌연변이발암원학회:학술대회논문집
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• 한국환경성돌연변이발암원학회 2001년도 Korean Environmental
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• pp.99-99
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• 2001

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.239-239
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• 2017

The enhancement of leaf photosynthetic capacity can have the potential to improve the seed yield of soybean. Key targets for the increase of leaf photosynthetic capacity remains unclear in soybean. Peking, Chinese local variety, has been the useful material for soybean breeding since it shows various resistances against biotic and abiotic stress. Sakoda et al., 2017 reported that Peking had the higher capacity of leaf photosynthesis than Enrei, Japanese elite cultivar. They identified the genetic factors related to high photosynthetic capacity of Peking. The objective of this study is to elucidate the physiological basis underlying high photosynthetic capacity of Peking. Peking and Enrei were cultivated at the experimental field of the Graduate School of Agriculture, Kyoto University, Kyoto, Japan. The sowing date was July 4, 2016. Gas exchange parameters were evaluated at the uppermost fully expanded leaves on 43, 49, and 59 days after planting (DAP) with a portable gas exchange system, LI-6400. The leaf hydraulic conductance, $K_{leaf}$, was determined based on the water potential and transpiration rate of the uppermost fully expanded leaves on 60 DAP. The morphological traits related to leaf photosynthesis were analyzed at the same leaves with the gas exchange measurements. The light-saturated $CO_2$ assimilation rate ($A_{sat}$) of Peking was significantly higher than that of Enrei at 43 and 59 DAP while the stomatal conductance ($g_s$) of Peking was significantly higher at all the measurements (p < 0.05). It suggested that high $A_{sat}$ was mainly attributed to high $g_s$ in Peking. $g_s$ is reported to be affected by the morphological traits and water status inside the leaf, represented by $K_{leaf}$, in crop plants. The tendency of the variation of the stomatal density between two cultivars was not consistent throughout the measurements. On the other hand, $K_{leaf}$ of Peking was 59.0% higher than that of Enrei on 60 DAP. These results imply that high $g_s$ might be attributed to high $K_{leaf}$ in Peking. Further research is needed to reveal the mechanism to archive high $g_s$ on the basis of water physiology in Peking. The knowledge combining the genetic and physiological basis underlying high photosynthetic capacity of Peking can be useful to improve the biomass productivity of soybean.

• 한국식품저장유통학회지
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• 제30권6호
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• pp.905-917
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• 2023

The optimal fermentation conditions for producing fermented black soybeans rich in sulfur-containing amino acids were investigated. Response surface methodology monitored fermentation conditions. A central composite design examined the effect of independent variables: enzyme concentration (X₁) and fermentation time (X₂) on yield and methionine content. Both factors significantly influenced these dependent variables. Enzyme concentration more profoundly affected amino nitrogen content than fermentation time. β-Glucan content and cystine level were primarily affected by fermentation time. We elicited each variable's regression formula and identified optimal fermentation conditions for functional compounds. The predicted optimum conditions were an enzyme concentration of 0.28-0.32% and a fermentation time of 58.0-62.0 min. Under these optimal conditions, each black soybean variety's sulfur amino acid content ranged from 818.62 to 922.62 mg/100 g, demonstrating significant variety differences.