• Title/Summary/Keyword: Soybean plant

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Improvement of a Black Soybean Line With Green Cotyledon and Triple Null Alleles for P34, 7S α' Subunit, and Lectin Proteins (P34, 7S α' Subunit 및 Lectin 단백질이 없는 녹색자엽을 가진 검정콩 계통 개발)

  • Sarath Ly;Sang In Shim;Min Chul Kim;Jin Young Moon;Jong Il Chung
    • Journal of Life Science
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    • v.34 no.5
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    • pp.313-319
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    • 2024
  • Cultivars or genetic resources with a black seed coat and green cotyledons are rich in lutein, which can promote eye health, and anthocyanin, known for its numerous health benefits. However, mature seeds also contain P34, 7S α' subunit, and lectin proteins, which are allergenic and degrade quality. Here, we report the breeding of a new soybean line with a black seed coat, green cotyledon, and free of P34, 7S α' subunit, and lectin proteins. A total of 157 F2 seeds with black seed coats and green cotyledons were selected by crossing a female parent with a brown seed coat, green cotyledon, and lacking the 7S α' subunit and lectin proteins with a male parent with a black seed coat, green cotyledon, and lacking the P34 and lectin proteins. The P34 and 7S α' subunit proteins were consistent with a ratio of 9:3:3:1, indicating that they are independent of each other. From 14 F2 seeds that were recessive (cgy1cgy1p34p34) for both proteins, one individual F2 plant (F3 seeds) with the desired traits-black seed coat, green cotyledon, and lacking P34, 7S α' subunit, and lectin proteins- was finally selected. The triple null genotype (absence for P34, 7S α' subunit, and lectin proteins) was confirmed in random F3 seeds. The selected line has a black seed coat and green cotyledons, and when sown on June 14 in the greenhouse, the maturity date was approximately October 3, the height was about 68 cm, and the 100-seed weight was about 26.5 g.

Nutritional Value and Digestibility of Tenebrio molitor As a Feed Ingredient for Rockfish (Sebastes schlegeli) (조피볼락의 사료원으로 갈색거저리의 영양적 가치 및 소화율 평가)

  • JANG, Tae-Ho;JUNG, Sung-Mok;KIM, Esther;LEE, Yong-Seok;LEE, Sang-Min
    • Journal of Fisheries and Marine Sciences Education
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    • v.29 no.3
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    • pp.888-898
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    • 2017
  • Tenebrio molitor larvae, also known as yellow mealworms (MW), are rich in protein and lipid and can serve as a potential alternative protein and energy source in commercial aquafeeds. Therefore, this study attempts to evaluate the effects of different drying methods on the nutritional value of MW meal. For this, live MW were cold-anaesthetized before being subjected to three different types of drying methods, including freeze-drying, oven-drying at $60^{\circ}C$ and air-drying at room temperature for three days, and compared for proximate composition and energy content. An in-vivo digestibility test was then conducted to evaluate the nutrient digestibility of MW meal in diets for rockfish, Sebastes schlegeli. A test diet was prepared by mixing the MW meal with a reference diet (Ref) in a 30:70 ratio with chromium oxide as an inert marker at the inclusion level of 0.5%. Rockfish with mean body weight of 150 g were stocked into a fecal collection system equipped with fiberglass tanks of 400 L capacity. Each group of fish was fed one of the experimental diets to apparent satiation for 4 weeks. The results of the proximate analysis showed that drying methods had no significant effect on crude protein, crude lipid, ash and energy contents of MW. Despite being a rich source of protein and lipid, MW meal was deficient in certain amino acids, particularly methionine, and highly unsaturated fatty acids, particularly 22:6n-3 (DHA) and 20:5n-3 (EPA). MW meal showed high digestibility values for protein (93%), lipid (97%) and energy (88%). These results may indicate that MW meal is a nutritious and acceptable feed ingredient, with comparable digestibility values to conventional animal and plant feedstuffs such as fish meal and soybean meal, in practical diet for rockfish at grower stage.

Insecticidal Activity of Bacillus thuringiensis 656-3 Strain to Mushroom Flies in Oyster Mushroom House

  • Park, Yong-Soo;Kang, Young-Jin;Lee, Kwang-Sik;Jianhong Le;Je, Yeon-Ho;Roh, Jong-Yul;Seo, Sook-Jae;Sohn, Hung-Dae;Jin, Byung-Rae
    • International Journal of Industrial Entomology and Biomaterials
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    • v.8 no.1
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    • pp.61-66
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    • 2004
  • Bacillus thuringiensis 656-3 which was isolated from a soil sample of mushroom house and showed high toxicity to mushroom flies, Lycoliella mali and Coboldia fuscipes, was surveyed for insecticidal effect in the oyster mushroom house.3. thutingiensis 656-3 was mass-cultured in the fermenter containing soybean cake(2%) and wheat bran (2%) as media source. Semi-for-mutation of B. thuringiensis 656-3 was performed with metamorphic starch only. When the formulation suspension containing $5{\times}{10^7}$ cfu was sprayed on the mushroom in mushroom house, the insecticidal effect of B. thuringiensis 656-3 to mushroom flies,1. mali and C. fuscipes, was maintained over 90% by the fifth day after starting spraying. The yield of oyster mushroom house with B. thuringiensis 656-3 was significantly increased compared to control. B. thuringiensis 656-3 represents a powerful biological insecticide for the control of mushroom flies.

A Study on the Literature of the Cooking Product of Cho(Misutkaru) in Imwonshibyukji ("임원십육지(林園十六誌)”의 초류(麨類) 조리 가공에 관한 문헌 연구 )

  • Kim Gwi-Young;Lee Choon-Ja
    • Journal of the East Asian Society of Dietary Life
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    • v.16 no.4
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    • pp.377-386
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    • 2006
  • Imwonshibyukji(1827) is an important and stupendous ancient scientific book, which is much like an encyclopedia, that can discern the dietary life of the latter period of Choseon. This article is assorted, which is mentioned in this book, as Cho(Misutkaru-in parched grain that is weed out into powder) to the main material of grain Youmicho, Wandoocho, Youcho, also the medicinal plant as the main material Youkhangcho, Backyupcho, Chungumcho, Unyoungcho, and Naecho, Haengcho, Eicho, Imgumcho, Joyou, Bokbunjacho, made by fruit. The results of inquiring the property by the assortment is as you see in the following. Youmicho, Wandoocho, Youcho, Youkhyangcho, Backyupcho, and Chungumcho, which consist of glutinous rice, nonclutinous rice, barley, buckwheat, pea, and black soybean are among 13 kinds of methods to make Cho. The manufacturing process is similar to that of Misutkaru, but in the case of Unyoungcho 6 different medicinal powders are mixed with Chundang and Sookmill to form a hard portable taffy substance, which is quite unique. Naecho, Haengcho, Eicho, Imgumcho, Joyou, and Bokbunjacho are extracted from pulp and processed in a powder form like Misutkaru, which can also be considered as a fruit tea. Youcho is unique in its sense, since it is fermented by maggots that are bred in order to obtain the juice. Cho is known to calm thirst and empty stomachs, and has various medicinal materials to act as food to aid virility. It is also known to have multiple functions as an alternative staple, refreshment to aid virility, portable food, and preferable beverage. There are ten references on Cho, 37% are Korean in Ongheejobji and 63% Chinese in Jaeminyosool, Bonchogangmok, Chungmoonbobang, Nongjungchunsuh, Wangshinongsuh, Shikgyung, Sukmyung, Bonchoseupyou, and Wisaengbogam. The citations are in the general introduction and 13 types of Cho are cited 19 times overall, but citations based on Chinese references are non existe.

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Desmutagenic Effect of Legumes and Plants Crude Saponins in Salmonella typhimurium TA 98 (콩과 식물에서 추출한 사포닌의 돌연변이원성 억제효과)

  • Ryu, Beung-Ho;Lee, Beung-Ho;Ha, Mi-Suck;Kim, Dong-Seuk;Sin, Dong-Bun;Nam, Ki-Du
    • Korean Journal of Food Science and Technology
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    • v.18 no.5
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    • pp.345-350
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    • 1986
  • Crude saponins of soybean and Azuki bean (2.0 mg/plate)were most effective against Trp-p-2, and also all of legume saponins (2.0 mg/plate)were excellent effective against aflatoxin $B_1$. Crude saponins of taro, burdock and ginseng were remarkably effective at ranging from 1.0 mg to 2.0 mg per plate against MeIQ. Especially ginseng saponin was excellent effective and arrow root saponins was remarkably effective against MeIQ, respectively. Plant saponins of taro, lotus burdock, arrow root and ginseng except for dodok were most effective activities against aflatoxin $B_1$.

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Identifying Factors for Corn Yield Prediction Models and Evaluating Model Selection Methods

  • Chang Jiyul;Clay David E.
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.50 no.4
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    • pp.268-275
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    • 2005
  • Early predictions of crop yields call provide information to producers to take advantages of opportunities into market places, to assess national food security, and to provide early food shortage warning. The objectives of this study were to identify the most useful parameters for estimating yields and to compare two model selection methods for finding the 'best' model developed by multiple linear regression. This research was conducted in two 65ha corn/soybean rotation fields located in east central South Dakota. Data used to develop models were small temporal variability information (STVI: elevation, apparent electrical conductivity $(EC_a)$, slope), large temporal variability information (LTVI : inorganic N, Olsen P, soil moisture), and remote sensing information (green, red, and NIR bands and normalized difference vegetation index (NDVI), green normalized difference vegetation index (GDVI)). Second order Akaike's Information Criterion (AICc) and Stepwise multiple regression were used to develop the best-fitting equations in each system (information groups). The models with $\Delta_i\leq2$ were selected and 22 and 37 models were selected at Moody and Brookings, respectively. Based on the results, the most useful variables to estimate corn yield were different in each field. Elevation and $EC_a$ were consistently the most useful variables in both fields and most of the systems. Model selection was different in each field. Different number of variables were selected in different fields. These results might be contributed to different landscapes and management histories of the study fields. The most common variables selected by AICc and Stepwise were different. In validation, Stepwise was slightly better than AICc at Moody and at Brookings AICc was slightly better than Stepwise. Results suggest that the Alec approach can be used to identify the most useful information and select the 'best' yield models for production fields.

Effects of $NH_4-N,\;NO_3-N$ and Urea-N on the Growth of Soybean Plants Different in Phosphorus Sensitivity (인산감수성(燐酸感受性)이 다른 대두(大豆)의 생육(生育)에 대(對)한 암모늄태(態) 초산태(硝酸態) 및 요소태질소(尿素態窒素)의 영향(影響))

  • Park, Hoon;Stutte, Charles A.
    • Applied Biological Chemistry
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    • v.16 no.3
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    • pp.118-127
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    • 1973
  • Effects of nitrogen sources oi phosphorus sensitivity in soybeans were investigated using three nitrogen source ($NH_4-N,\;NO_3-N$ and urea) and six cultivars (Lee, Hill, R56-49, Harosoy, Clark-63 and Chippewa), differing in phosphorus sensitivity. Growth inhibition with ammonium or urea medium increased with phosphorus sensitivity indicating that the phosphorus sensitive cultivars are also sentitive to ammonium. The sensitive Clark-63 was inhibited two folds more than the tolerant Lee in ammonium medium. These results strongly suggest that phosphorus sensitivity is closely linked to nitrogen metabolism and ammonium toxicity. Nitrogen sources gave special morphorogical growth patterns (short internode-many branchs in $NO_3-N$, long internode-no branches in $NH_4-N$ and a low top to root ratio in urea) by affecting differently the growth of plant organs.

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A Study on Effect and Significance of Food Taboo on Korean Food Life Style (금기식(禁忌食)이 식생활(食生活) 양식(樣式)에 미치는 영향(影響) 및 의의연구(意義硏究))

  • Park, Mo-Ra;Hwang, Choon-Sun
    • Journal of the Korean Society of Food Culture
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    • v.3 no.3
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    • pp.245-258
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    • 1988
  • A food life style itself is substrative relations with culture, and is concerned with our daily life. Especially a food taboo comes from the intelligence which resulted from an ancestor's life experience. Accordingly, we can say a food taboo began with human appearance, settled in convention of folklore society, and forms it's own boundary todays. Since a practice of a food taboo is combinded firmly with various sociological factors such as religion, custom, and so on, it is very hard to change, even though the belife of a food taboo is not true. According to the result, first, significant factors effecting on a practice of a food taboo was a level of age, a level of education, religion, family pattern, behavior of subject and her mother for food life management, education of subject and her mother, and religion of subject and her mother. Second, in analysis of factors according to classification of food taboo a practice of a food taboo which classified to food of animal, food of plant and the other food showed significant differences, comparing religion of subject with her mother. Third, a result examined practice frequency and review of science, non science indicated that foods such as Egg of Globefish, Lettuce, Coffee, Persimmon, dried Persimmon, Soybean and Sugar are tabooed on the basis of science, and foods such as Vinegary food, Thieved food, Soup of Tangle and Dogmeat tabooed on the basis of non science. But in the case of Puja, it's basis of science is not identified. These food taboos are tabooed by more then 50% of subjects including who answerd 'there is some case to practice it' Therefore, we should continue analysis of science for the reason of a food taboo, at the same time, provide the origin and try to have a rational food life.

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A Determination of the Salicylate Content of Ingredients Commonly Used in Korean foods Suggested for Allergic Patients

  • Myung, Choonok;Lee, Kiwan;Nam, Haewon;Hong, Chein-Soo;Lee-Kim, Yang-Cha
    • Journal of Nutrition and Health
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    • v.31 no.5
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    • pp.949-956
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    • 1998
  • Salicylate from plant foods and histamine from animal foods have been suggested to cause various food allergic symptoms. Salicylate regarded as one of the inducing agents f3r chronic urticaria and asthma in allergic patients was studied. There is much interest about the effects of salicylate in food allergy. It it recently suggested that the intake of salicylate from foods may have contributed to chronic urticaria and asthma. The purpose of this study is to analyze the salicylate content in various foods. 153 ingredients commonly used in Korean foods were analyzed by HPLC. Among the vegetables, chard(8.22$\mu\textrm{g}$/g), soybean sprout(5.13$\mu\textrm{g}$/g), perilla lean3.03$\mu\textrm{g}$/g), Chinese cabbage(2.25$\mu\textrm{g}$/g), carrot(2.16$\mu\textrm{g}$/g), and squash(1.33$\mu\textrm{g}$/g) con-rained higher amounts of salicylate as compared to other vegetables. Among fruits, oranges(5.13$\mu\textrm{g}$/g), grapefruits(2.90$\mu\textrm{g}$/g), and plums(1.65$\mu\textrm{g}$/g) contained the most. In animal foods, almost no salicylate was found except in chocolate milk(0.32$\mu\textrm{g}$/g). It was also found in black pepper(8.2$\mu\textrm{g}$/g), coffee(can)(5.00$\mu\textrm{g}$/g), mustard(4.16$\mu\textrm{g}$/g), curry powder(3.24$\mu\textrm{g}$/g), kochujang(2.37$\mu\textrm{g}$/g), cream powder(1.19$\mu\textrm{g}$/g), brown rice(1.04$\mu\textrm{g}$/g) and sesame oil(1.00$\mu\textrm{g}$/g). All information found in this study can be used as nutritional education materials in potentially allergic people for a prudent diet plan and can also provide proper dietary guidelines for these allergic patients. (Korean J Nutrition 31(5) 949-956, 1998)

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Estimate of Vitamin $B_6$ Intake and Major Dietary Sources of Vitamin $B_6$ in Children Aged 7-12 Years in the Seoul Area

  • Cho, Youn-Ok;Park, Sung-Sook
    • Nutritional Sciences
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    • v.7 no.2
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    • pp.98-106
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    • 2004
  • The dietary vitamin $B_6$ intake of 185 Korean children aged 7-12 years (y), who showed no health problems, in the Seoul area and its sources were estimated using a modified Korean vitamin $B_6$ database. The age and sex of each subject was classified according to the classifications in the Korean Recommended Dietary Allowance (RDA). Dietary vitamin $B_6$ intake and food sources were estimated using the three-day recall method with the help of a trained interviewer. Food portion sizes were estimated by using standard household measures and published average portion sizes. The average daily vitamin $B_6$ intake was 1.69$\pm$0.50 mg/d in children aged 7-9 y, 1.86$\pm$0.44 mg/d in male children aged 10-12 y and 1.77$\pm$0.62 mg/d in female children aged 10-12 y. Less than 5% of the subjects consumed less than the Korean RDA of vitamin $B_6$. The average ratio of vitamin $B_6$intake to daily protein intake was 0.028$\pm$0.006 mg/g in children aged 7-9 y, 0.028$\pm$0.004 mg/g in male children aged 10-12 y and 0.029$\pm$0.007 mg/g in female children aged 10-12 y. The intake of vitamin $B_6$ was significantly (p<.01) positively correlated to the intake of all other nutrients. Foods from animal and plant sources provided 37% and 73% respectively, of total vitamin $B_6$. Major dietary sources of vitamin $B_6$ in children in the Seoul area were rice, soybean sprouts, pork, beef, cereal, kimchi, milk, onions, and potatoes. As for major dietary sources of vitamin $B_6$, the top 20 foods provided nearly 73-75% of the total vitamin $B_6$ consumed by Korean children.