• 식물조직배양학회지
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• 제27권6호
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• pp.491-496
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• 2000

더덕의 효과적인 재분화조건 (약 90%의 재분화율)을 바탕으로 개화시기 조절유전자인 RAG25 Codonopsis lanceolata유전자의 도입을 시도하였다. 더덕의 잎 절편을Agrobacterium과 3일 동안 공조배양 하였으며 NAA 2 mg/L, BA 2 mg/L (N2B2)와 NAA 2 mg/L, BA 4 mg/L (N2B4)가 첨가된 shoot inducing media (Km 20mg/L와 Cf 250 mg/L가 첨가됨)에서 배양하였을 때 형질전환 신초가 형성되었다. 획득된 신초에서 genomic DNA를 추출하고 npt II와 RAG25 primer를 이용한 PCR을 수행하여 각각 0.7 kb와 0.6 kb의 band를 확인하였다. RAG25 유전자에 대한 PCR산물을 이용하여 Southern hybridization을 수행한 결과 PCR과 동일한 위치에서 band 가 검출되어져 이 유전자의 도입을 재확인하였다.

• Journal of Forest and Environmental Science
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• 제25권1호
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• pp.35-41
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• 2009

Agroforestry combines agriculture and forestry technologies to create more integrated, diverse, productive, profitable, healthy and sustainable land-use systems. This study was performed in three union of Chhagalnaiya Upazila (Sub-district; administrative entity) under Feni district, Southern Bangladesh with a view to identify the tree resources, utilization pattern and economic return of major fruit and timber tree species. Information collected from a total of 45 households ranging from marginal, small, medium and large categories. Number of plant species increased with the increase of homestead area. A total of 39 plant species were recorded from the homegarden, of which 23 were fruit and 16 were timber tree species. Considerable number of vegetables was also planted under the shade of the homestead trees. The investment analysis showed that average benefit-cost ratios were greater than one, net present values were positive and internal rate of returns were more than 10%. Long term investment on horticulture and timber tree species is highly profitable if species like Swietenia mahagoni and Tectona grandis, Spondias pinnata, Syzygium cumini and Areca catechu were planted.

• 식물분류학회지
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• 제40권4호
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• pp.208-217
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• 2010

Crossosomatales is a recently recognized order in the rosid II clade with about 64 species in eight morphologically distinct families that have been previously classified in as many as 15 other orders. Phylogenetic relationships among the families and genera within Crossosomatales were investigated using chloroplast atpB, matK, and rbcL sequences employing maximum parsimony, maximum likelihood, and Bayesian methods. The phylogenetic framework was used to examine the patterns of morphological evolution and synapomorphies for subclades within Crossosomatales. The combined data with representative species from all genera in the order strongly supported monophyly of Crossosomatales. Strong support was found for the families in the Southern Hemisphere, in which Aphloiaceae is sister to the clade of (Geissolomataceae, (Ixerbaceae + Strasburgeriaceae)). The sister relationship between the Southern Hemisphere clade and families distributed primarily in the Northern Hemisphere was also supported. As in the previous studies, following relationships were found within the Northern Hemisphere clade: Staphyleaceae is sister to a clade of (Guamatelaceae, (Stachyuraceae + Crossosomataceae)). The pattern analysis indicates that evolutionary pattern of morphological characters is complex, requiring multiple changes within Crossosomatales. Several reproductive traits, such as inflorescence, aril, stigma, and conspicuous protrusion from pollen aperture, corroborate the molecular phylogeny.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2022년도 추계학술대회
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• pp.150-150
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• 2022

Plant reproduction associated with crop yields is highly vulnerable to global climate change components such as high and cold temperatures. The objectives of this study were to determine the effects of season-high exposure to temperature treatments in pollen morphology on Adzuki bean (V. angularis) and Mung bean (V. radiate). V. angularis and V. radiata were treated at high temperatures in the high temperature gradient greenhouse designed to cause temperature deviation. The pollen shapes treated at high temperature were compared by an electron scanning microscope. As a result, it was confirmed that the number of abnormal pollens morphology at high temperature was the least in V. radiata, and V. angularis was vulnerable to high temperatures. Also, it was found that the number of abnormal pollen morphology atT4 (Con +5~6℃) varied according to the cultivars of V. angularis. Therefore, the differences in Vigna species or cultivars with thermo-tolerance in pollen morphology to high temperature are projected to occur in the changeable future climate.

• The Plant Pathology Journal
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• 제29권4호
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• pp.440-445
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• 2013

Balloon flower (Platycodon grandiflorum) is a kind of mountain herbs whose roots have restorative properties and the cultivating acreage of balloon flower has been steadily increasing in Korea. More frequent rain and high amount of rainfalls as a result of climate changes predisposed balloon flower to the outbreaks of root rot at high-density cultivation area in recent years. Root crowns were usually discolored into brown to blackish brown at first and the infected plants showed slight wilting symptom at early infection stage. Severely infected roots were entirely rotted and whole plants eventually died at late infection stage. The overall disease severities of root rot of balloon flower were quite variable according to the surveyed fields in Jeonnam, Gyeongnam and Jeju Provinces, which ranged from 0.1% to 40%. The root rot occurred more severely at the paddy or clay soils than the sandy soils and their severities were much higher at lowland than upland in the same localty. The disease increased with aging of the balloon flower. The causal fungi were identified as Fusarium solani and F. oxysporum on the basis of their mycological characteristics. The optimum temperature ranges of their mycelial growths was found to be $24^{\circ}C$. The pathogenic characters of F. solani and F. oxysporum treated by artificial wounding inoculation on healthy roots of balloon flower revealed that F. solani was more virulent than F. oxysporum. This study identified the causal agents of root rot of balloon flower as Fusarium solani and F. oxysporum, probably for the first time.

• 식물분류학회지
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• 제49권4호
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• pp.324-333
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• 2019

Neutral genetic diversity found in plant species usually leaves an indelible footprint of historical events. Korea's main mountain range (referred to as the Baekdudaegan [BDDG]), is known to have served as a glacial refugium primarily for the boreal and temperate flora of northeastern Asia. In addition, life-history traits (life forms, geographic range, and breeding systems) influence the within- and among-population genetic diversity of seed plant species. For example, selfing species harbor significantly less within-population genetic variation than that of predominantly outcrossers. A previous study of two Liparis species (L. makinoana and L. kumokiri) emphasizes the role of the abovementioned factors shaping the levels of genetic diversity. Liparis makinoana, mainly occurring on the BDDG and self-incompatible, harbors high levels of within-population genetic diversity (expected heterozygosity, H_eP = 0.319), whereas there is no allozyme variation (H_eP = 0.000) in L. kumokiri, which is self-compatible and mainly occurs in lowland hilly areas. To determine if this trend is also found in other congeners, we sampled five populations of L. krameri from the southern part of the Korean Peninsula and investigated the allozyme-based genetic diversity at 15 putative loci. The somewhat intermediate levels of within-population genetic variation (H_eP = 0.145) found in L. krameri are most likely due to its occurrence in mountainous areas that, despite being outside of the main ridge of the BDDG, still served as refugia, and a self-incompatible breeding system. Management strategies are suggested for L. krameri and L. makinoana based on the levels and distribution of genetic diversity and inbreeding.

• 한국환경생태학회지
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• 제3권1호
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• pp.16-27
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• 1989

가야산 국립공원지역내에 분포되어 잇는 관속식물을 파악하고자 본 조사를 수행하였으며, 총 96종과 465종, 72변종 및 4품종으로 조사되었다. 본 조사시 밝혀진 식물을 분포유형으로 구별해 보면. 중남부분포형이 10과 11종으로 가장 많았으며, 다음으로 전한국분포형이 9과 12종으로 나타났다. 과 및 속의 수준에서 볼 때에 본 가야간 국립광원의 식물분포유형은 중남부분포형이 가장 많았으며. 이 중에서 난대형(1과 및 5속)과 남해안 도서분포형 및 북부형이 각각 1과씩 분포되어 있는 것이 특이하였다.

• Animal cells and systems
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• 제2권2호
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• pp.243-248
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• 1998

We have partially sequenced glutaminyl cyclases from several mammalian and one avian species and found that the two cysteine residues of the human glutaminyl cyclase are completely conserved. The mammalian glutaminyl cyclase has been reported to possess reactive thiols (Busby, Jr, et aI., 1987, J BioI Chern 262, 8532-8536). Mutagenesis of these cysteine residues, however, resulted in only a slight decrease in enzyme activity. Likewise, the recombinant human enzyme was completely resistant to attempted chemical modification of the putative reactive thiols. Although the human glutaminyl cyclase did not appear to have reactive thiols, it was sensitive to diethylpyrocarbonate and acetylimidazole, indicating the presence of functionally important histidine and tyrosine residues which could act as acid/base catalysts. Almost identical deuterium solvent isotope effect (1.2 vs 1.3) upon the reaction by the human and papaya enzymes, respectively, provides an evidence both animal and plant glutaminyl cyclases catalyze pyroglutamyl-peptide formation by intramolecular cyclization.

• 대한공간정보학회지
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• 제25권2호
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• pp.13-19
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• 2017

2006년부터 2013년까지의 환경위성 자료인 MODIS자료를 대상으로 기상변화와 식생지수와의 관계를 분석하였다. 실험지역은 한반도를 북부, 중부 및 남부로 나누어 지역특성에 따라 분석하였으며, Timesat을 활용하여 평활화된 MODIS LAI 및 NDVI를 획득하였다. 강수량과 MODIS NDVI의 경우 0.66, 0.44, 0.35로 MODIS LAI보다 높은 상관성을 나타냈으며, 북부에서의 상관성이 가장 높았다. 기온의 경우 MODIS LAI는 북부, 중부 및 남부에서 0.66, 0.64, 0.68의 상관계수값을 가졌고, MODIS NDVI의 경우 0.89, 0.89, 0.80의 상관계수 값을 나타냈다. MODIS NDVI의 상관성이 더 높았으며, 지역에 상관없이 유사한 양의 상관성을 나타냈다. 또한 Timesat을 통해 획득한 식물계절의 시작일과 실제 식물계절의 시작일을 비교한 결과, MODIS NDVI의 정확도가 높았다. MODIS LAI에서의 오차의 경우 중부에서 평균 19일, 남부에서 평균 20일이었고, MODIS NDVI에서의 오차의 경우 중부에서 평균 6일, 남부에서 평균 8일을 나타냈다.

• Journal of Plant Biology
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• 제17권3호
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• pp.107-112
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• 1974

Leaf temperature is complicated with the microclimate and the dry matter production in a plant community. But a daily change of leaf temperature varying by the locality in plant body or plant community is not yet clear. To resolve such a question, following experiment was designed; Helianthus tuberosus L., Glycine max L., Zea mays L., Impomoea batatas Lam., and Cucurbita moschata var. toonas Makino were planted in the different sandy loam, $2m{\times}2m-quadrat$, which has a eastern, southern, western and northern edge. In each plot 17-25 plants were planted and the distances between individuals spaced uniformly. And leaf temperature were measured by MR3-C type thermistor from 14th May through 20th August. It is seems that the upper leaf is affected by solar radiation, the lower leaf by released heat from the earth and the middle leaf by the conditions of both zones. Measuring the temperature of a leaf that is on terminal, central, left and right margins and base part, temperature of control plant in a leaf was sloped during about two hours from noon. It is noticeable as a "noon sleeping" phenomenon.henomenon.