• Proceedings of the Korean Society of Crop Science Conference
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• 2022.10a
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• pp.150-150
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• 2022

Plant reproduction associated with crop yields is highly vulnerable to global climate change components such as high and cold temperatures. The objectives of this study were to determine the effects of season-high exposure to temperature treatments in pollen morphology on Adzuki bean (V. angularis) and Mung bean (V. radiate). V. angularis and V. radiata were treated at high temperatures in the high temperature gradient greenhouse designed to cause temperature deviation. The pollen shapes treated at high temperature were compared by an electron scanning microscope. As a result, it was confirmed that the number of abnormal pollens morphology at high temperature was the least in V. radiata, and V. angularis was vulnerable to high temperatures. Also, it was found that the number of abnormal pollen morphology atT4 (Con +5~6℃) varied according to the cultivars of V. angularis. Therefore, the differences in Vigna species or cultivars with thermo-tolerance in pollen morphology to high temperature are projected to occur in the changeable future climate.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.96-96
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• 2017

Rice is a staple food for nearly half of the world's population, with more than 10,000 rice varieties providing almost one-quarter of the global per capita dietary energy supply. Grain size, panicle size and branch number, grain number in a panicle are directly associated with rice productivity. Recently several genes which increase grain yield were identified through map-based cloning. Gn1a, Cytokinin oxidase, is a major grain number QTL and regulates grain number per panicle. Dep1 increases panicle branching and reduced rachis length. SCM2 (APO1) was identified by a QTL for culm strength and increased spikelet number. OsSPL16 (GW8) controls grain size and shape and then increases 1000-weight of seed. In here, to identify genotype of genes related to yield in 400 of rice germplasms possessed in National Institute of Crop Science, we had first chosen 4 of well-known genes related to yield; Gn1a, Dep1, SCM2, and OsSPL16. Among these germplasms, 195, 382, 165, and 353 of germplasms harbored the dominant type of Gn1a, Dep1, SCM2, and OsSPL16, respectively. We grouped these germplasms into a total of 10 groups using genotypes of Gn1a, Dep1, SCM2 and OsSPL16. Most rice germplasms belong to group 1, harbored Gn1a, dep1, gw8 and APO1, and group 10, harbored gn1a, Dep1, GW8 and apo1. Hanareum2 is the highest productive cultivar in Korea but do not have dominant type OsSPL16, so belong to group 1. On the other hand, in the case of Unkwang, belongs to group 10, which has dominant type of OsSPL16 but do not have the remaining genes. We can grasp the differences in rice germplasms through the Profiling of genes related to these grain yield, which will be useful for cross-breeding to integrate grain yield genes. We are continuously observing the genotype and phenotype of rice that possesses grain yield genes.

• Plant Breeding and Biotechnology
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• v.6 no.4
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• pp.404-412
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• 2018

The influenza neuraminidase (NA, E.C. 3.2.1.18), an antiviral, has been the target of high pharmaceutical companies due to its essential role in viral replication cycle. Perilla frutescens (P. frutescens) is used in traditional Chinese medicine for various diseases, such as cold due to wind-cold, headache and cough. In this context, four major polyphenolic compounds including rosmarinic acid-3-O-glucoside (1), rosmarinic acid (2), luteolin (3), and apigenin (4) isolated from P. frutescens were evaluated for their inhibitory effect on recombinant virus H1N1 neuraminidase (rvH1N1 NA). Among the test compounds, rosmarinic acid and luteolin inhibited the rvH1N1 NA with an $IC_{50}$ of 46.7 and $8.4{\mu}M$, respectively. The inhibition kinetics analyzed by the Dixon plots indicated that rosmarinic acid and luteolin were noncompetitive inhibitors and that the inhibition constant, $K_I$, was established as 43.9 and $14.3{\mu}M$, respectively. In addition, 578 genetically diverse accessions and 39 cultivars of P. frutescens were analyzed using HPLC to characterize the diversity of polyphenolic composition and concentration. The individual and total compositions exhibited significant difference (P < 0.05), especially rosmarinic acid which was detected as the predominant metabolite in all accessions (58.8%) and cultivars (62.8%). Yeupsil and Sangback cultivars exhibited the highest rosmarinic acid ($3,393.5{\mu}g/g$) and luteolin ($383.3{\mu}g/g$) content respectively. YCPL177-2 with the high concentration ($889.8{\mu}g/g$) of luteolin may be used as a genetic resource for breeding elite cultivars.

• Journal of The Korean Society of Agricultural Engineers
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• v.54 no.6
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• pp.19-28
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• 2012

This study carried out farmland suitability analysis for southern-type garlic cultivation considering soil and temperature as well as water deficit conditions. The spatial extent was limited within the area derived by Kim et al. (2012) using just soil and temperature constraints. Daily soil moisture was simulated using a one-layer soil water balance model at a $100{\times}100m$ grid unit, and then annual water deficit was calculated from 2000 to 2010. The farmland suitability was classified as four steps: best suitable, suitable, possible, and low productive. As a result, total area of best suitable or suitable farmland was about 375,900 ha, and Gimje-si and Haenam-gun were appeared as the largest favorable area for southern-type garlic cultivation. The best suitable or suitable area at Haenam-gun, Goheung-gun, Shinan-gun, Namhae-gun, and Muan-gun, major production regions of southern-type garlic, were extracted as 20,187 ha, 13,018 ha, 4,715 ha, 1,319 ha, and 349 ha, respectively. On the other hand, the result showed that the adoption of sprinkler irrigation systems might be critical in cultivating the southern-type garlic at some regions having poor water balance.

• Korean Journal of Veterinary Service
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• v.32 no.4
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• pp.369-375
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• 2009

This study was attempted to survey the prevalence of heartworm infection among 253 dogs (male 167, female 86) on the 16 breeding farms in southern Gyeongnam area in May to December 2007. The results obtained from this experiment were summarized as follows; Fifty seven (22.5%) of the 253 examined dogs were heartworms-antigen positive, while twenty five dogs were Microfilaria positive by the modified Knott's method. The regional infection rates were of 25% in Tongyeong and 21% in Goseong, and on the breeding environment aspects, infection rates were lower in rural area than urban area farms. The infection rates were higher in female (26/86, 30.2%) than male dog (31/167, 18.6%). The infection rates by breeding size of farms were 31% (11/35) at <20 head, 26%(35/135) at 20~50 head and 13% (11/82) at >50 head. The infection rates of heartworm in examined dogs at the age of <2, 2~4 and >5 were 10.7% (8/75), 26% (40/154) and 37.5% (9/24), respectively. In the microfillaria-infected 25 dogs, identified Dirofilaria immitis of 21 (84%) using PCR. The dogs infected with heartworm showed the increased number of eosinophil.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.210-210
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• 2017

Sorghum is rich source of various phytochemicals including phenolic acids that have potential to significantly impact human health. Phytochemical production may be induced by not only genotype but a number of environmental factors including temperatures and amount of sunshine. The objective of this study was to determine the effect of planting date and harvesting stage on the agronomic and quality traits of 'Donganme' grain sorghum variety developed to produce high antioxidant activity. 'Donganme' were planted in three locations at four dates from early May to early July. Each planted fractions were harvested five times 35, 40, 45, 50, 55 days after head shooting date, respectively. Significant difference existed between the growth period and the agronomic traits. The interaction effects planting date and harvesting date was significant for plant height, tiller production, grain yield and antioxidant activity, indicating that low temperature and integrated sunshine influence on that traits. The result showed that antioxidant activity production occurred when the sorghum crop was grown in late season although the yield is lower. To produce antioxidant activity from sorghum grain need to consider the relation between the yield and nutrition component simultaneously.

• Proceedings of the Korean Society of Crop Science Conference
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• 2022.10a
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• pp.231-231
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• 2022

The GASA protein (Gibberellic acid-stimulated Arabidopsis) are family of small cysteine-rich peptides found in plants. These GASA gene family mainly involved in biotic/abiotic stress responses and plant development. Despite being present in a wide plant species, their action and functions still remain unclear. In this study, using the in-silico analysis method we identified 41 GASA genes in peanuts (Arachis hypogaea L.). Based on the phylogenetic analysis 41 GASA genes are classified in the four major clusters and subclades. Mainly, clusters IV and III comprise the majority of GASA genes 15 and 11 genes respectively, followed by cluster I and cluster II with 9 and 6 genes respectively. Additionally, based on in-silico analysis we predicted the post-transcriptional and post-translational changes of GASA proteins under abiotic stresses such as drought and salt stress would aid our understanding of the regulatory mechanisms. Hence, a further study is planned to evaluate the expression of these GASA genes under stress in different plant tissues to elucidate the possible functional role of GASA genes in peanut plants. These findings might offer insightful data for peanut advancement.

• Proceedings of the Korean Society of Crop Science Conference
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• 2018.10a
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• pp.150-150
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• 2018

• Proceedings of the Korean Society of Crop Science Conference
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• 2023.04a
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• pp.23-23
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• 2023

• Proceedings of the Korean Society of Crop Science Conference
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• 2022.10a
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• pp.20-20
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• 2022

A traditional wheat breeding program needs more than 12-13 years to develop a new cultivar. In recent years, 'Speed breeding (SB)' system, which uses extremely extended photoperiod (22 h), enabled up to 4-6 generations of spring wheat per year. However, since almost 70% of wheat cultivars are winter type, and over 95% of total cultivation area is for winter wheat in Korea, optimized vernalization treatment was essential for improving the SB system. Several vernalization temperatures and durations were tested with various genotypes, and the 4 weeks of 8-10 ℃ vernalization treatment was the most effective to develop 4 generations per year, for both spring and winter type wheat cultivars. This 'Speed vernalization (SV)' system followed by SB, allowed developing a new F₆ recombinant inbred lines (RILs) within 2 years. Among the 184 RILs, which derived from a cross between Jokoyung and Joongmo2008, two outstanding lines were selected for yield trial test, and then named Milyang52 and Milyang53. Compared to the traditional wheat breeding program, over 60% of the time was saved to develop these two lines. Marker-assisted selection and backcross were also combined with the SV system. YW3215-2B-1 (Jokoyung*3/Gamet), which has similar agronomic traits with Jokyoung and the same Glu-B1 allele with Garnet, was developed within 2.5 years. Thus, the SV system combined with molecular breeding technology would help breeders to make a new cultivar with less time and high efficiency.