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QTLs analysis associated with a major agronomic traits in hanareum2×unkwang rice recombinant inbred line

  • Lee, Ji Yoon;Cho, Jun Hyeon;Kang, Ju Won;Shin, Dong Jin;Kim, Tae Heon;Song, You Chun;Han, Sang Ik;Park, Dong Soo;Son, Young Bo;Cho, Su Min;Oh, Myeong Kyu
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2017.06a
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    • pp.101-101
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    • 2017
  • This study was carried out to improve yield potential of Tongil type rice variety based on QTLs analysis associated with yield component using a total of 386 rice recombinant inbred lines (RILs) derived from a cross between Tongil type high yield variety "Hanareum2" and Japonica variety "Unkwang". 384 SNP markers were used, and 241 of them (62.6%) were polymorphic between Hanareum2 and Unkwang. One hundred forty-four QTLs in 11 traits, such as heading days, were detected. Most of them were 21 QTLs associated with 1000 grain weight and the least was 8 QTLs associated with panicle number. The QTL, qDTH3-2 associated with days to heading was identified to delay heading date for 2.4~2.6 day. Eleven QTLs were associated with culm length. The QTL, qCL1-2 on chromosome 1, was identified to decrease culm length. A total of 16 QTLs were detected for panicle length. Three QTLs, qPL3, qPL6, and qPL7-1 were increased panicle length. Seven QTLs related to panicle number except qPN7 were increased the number of panicle. Four QTLs related to grain number per panicle, qGNP2-1, qGNP6, and qGNP7, were increased the number of grains. Three QTLs associated with grain filling rate, qGFR1, qGFR2-2, and qGFR7-1 were increased grain filling rate. Twelve QTLs associated with 1,000 grain weight. were increased the grain weight. Fourteen QTLs were identified associated with grain length. 10 QTLs, such as qGL1-1, were increasing grain. Fifteen QTLs associated with grain width were detected. The 8 QTLs, such as qGW1-1, were elongated grain width. Seventeen QTLs were associated with grain thickness, and ten QTLs of them were increased grain thickness. We need further study to develop introgression lines of each QTL to improve yield potential of Tongil type rice variety.

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Analysis of microRNA expression profiles during the cell cycle in synchronized HeLa cells

  • Zhou, Jue-Yu;Ma, Wen-Li;Liang, Shuang;Zeng, Ye;Shi, Rong;Yu, Hai-Lang;Xiao, Wei-Wei;Zheng, Wen-Ling
    • BMB Reports
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    • v.42 no.9
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    • pp.593-598
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    • 2009
  • Cell cycle progression is regulated by both transcriptional and post-transcriptional mechanisms. MicroRNAs (miRNAs) emerge as a new class of small non-coding RNA regulators of cell cycle as recent evidence suggests. It is hypothesized that expression of specific miRNAs oscillates orderly along with cell cycle progression. However, the oscillated expression patterns of many candidate miRNAs have yet to be determined. Here, we describe miRNA expression profiling in double-thymidine synchronized HeLa cells as cell cycle progresses. Twenty-five differentially expressed miRNAs were classified into five groups based on their cell cycle-dependent expression patterns. The cyclic expression of six miRNAs (miR-221, let-7a, miR-21, miR-34a, miR-24, miR-376b) was validated by real-time quantitative RT-PCR (qRT-PCR). These results suggest that specific miRNAs, along with other key factors are required for maintaining and regulating proper cell cycle progression. The study deepens our understanding on cell cycle regulation.

TGF-β1 Protein Expression in Non-Small Cell Lung Cancers is Correlated with Prognosis

  • Huang, Ai-Li;Liu, Shu-Guang;Qi, Wen-Juan;Zhao, Yun-Fei;Li, Yu-Mei;Lei, Bin;Sheng, Wen-Jie;Shen, Hong
    • Asian Pacific Journal of Cancer Prevention
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    • v.15 no.19
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    • pp.8143-8147
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    • 2014
  • To investigate the expression intensity and prognostic significance of TGF-${\beta}1$ protein in non-small cell lung cancer (NSCLC), immunohistochemistry was carried out in 194 cases of NSCLC and 24 cases of normal lung tissues by SP methods. The PU (positive unit) value was used to assess the TGF-${\beta}1$ protein expression in systematically selected fields under the microscope with Leica Q500MC image analysis. We found that the TGF-${\beta}1$ PU value was nearly two-fold higher in NSCLC than in normal lung tissues (p=0.000), being associated with TNM stages (p=0.000) and lymph node metastases (p=0.000), but not to patient age, gender, smoking history, tumor differentiation, histological subtype and tumor location (P>0.05). Univariate analysis indicated that patients with high TGF-${\beta}1$ protein expression and lymph node metastases demonstrated a poor prognosis (both p=0.000,). Multivariate analysis showed that TGF-${\beta}1$ protein expression (RR = 2.565, p=0.002) and lymph node metastases (RR=1.874, p=0.030) were also independent prognostic factors. Thus, TGF-${\beta}1$ protein expression may be correlated to oncogenesis and serve as an independent prognostic biomarker for NSCLC.

Effect of different transplanting and harvest times on yield and quality of pigmented rice cultivars in the Yeongnam plain area

  • Kim, Sang-Yeol;Han, Sang-Ik;Oh, Seong-Hwan;Seo, Jong-Ho;Yi, Hwi-Jong;Hwang, Jung-Dong;Choi, Won-Yeong;Oh, Myung-Kyu
    • Korean Journal of Agricultural Science
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    • v.43 no.3
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    • pp.330-339
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    • 2016
  • The effect of transplanting and harvest timing was evaluated for the production of high quality pigmented rice in the Yeongnam plain area. Rice was transplanted on June $2^{nd}$ and $14^{th}$ and harvested between 35 - 55 days after panicle heading at 5 - day intervals. Three black- and 3 red-pigmented rice cultivars (such as early cultivar : Josengheugchal, Jeogjinju; medium cultivar : Heugseol, Hongjinju; and mid-late cultivar : Sintoheugmi, Geongganghongmi) were studied. Yield components like spikelet number, ripened grain ratio, and 1,000 - grain weight of the black- and red-pigmented rice cultivars were similar for both the June 2 and June 14 transplantings but panicle number per $m^2$ was higher for the June 14 transplanting than for June 2. This contributed to a higher brown rice yield for the June 14 transplanting, by 6 - 19% for black-pigmented rice, and by 10 - 21% for red-pigmented rice than the yield for the June 2 transplanting. Total anthocyanin and polyphenol productions of the pigmented rice were also higher in the June 14 transplanting than that in the June 2 transplanting due to high brown rice yield. Based on the combined pigmented brown rice yield, we concluded that the optimal harvest timing would be 40 - 45 days after panicle heading (DAH) for the black-pigmented rice and 45 - 50 DAH for the red-pigmented rice. This study suggests that optimum transplanting and harvest timings play an important role for production of high quality pigmented rice in the Yeongnam plain area.

The Ecological Characteristics by Slope of an Abies koreana Forest in Seseok of Jirisan National Park (지리산 세석 구상나무림의 사면별 생태적 특성)

  • Noh, Il;Chung, Jae-Min;Kim, Tae-Woon;Tamirat, Solomon;Moon, Hyun-Shik
    • Journal of Climate Change Research
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    • v.9 no.3
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    • pp.293-302
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    • 2018
  • This study was carried out to provide basic information on efficient preservation and management of an Abies koreana forest through analyzing the ecological characteristics by slope in Seseok, Mt. Jirisan. Soil moisture content at southern and northern slopes was 29.9% and 21.7%, respectively, and there was no significant difference among soil properties between southern and northern slopes. The importance value of A. koreana in the southern and northern slopes was high for tree and subtree layers, respectively. It is noteworthy that many seedling and saplings of A. koreana were present on the southern slope. The species diversity was 0.413 for the tree layer, 0.632 for the subtree layer, and 0.609 for the shrub layer on the southern slope and 0.396 for the tree layer, 0.783 for the subtree layer, and 1.215 for the shrub layer on the northern slope. Evenness and dominance ranged from 0.371 to 0.609 and 0.629 to 0.391 on the southern slope and from 0.380 to 0.968 and 0.620 to 0.032 on the northern slope, respectively. The mortality of A. koreana was 9.6% on the southern slope and 24.4% on the northern slope, a distinct difference between the slopes. The mortality type at two slopes was the highest proportion of standing dead. Annual mean tree ring growth of A. koreana on the southern slope (1.76 mm/yr) was higher than that on the northern slope (1.64 mm/yr).

Analysis of Molecular Variance and Population Structure of Sesame (Sesamum indicum L.) Genotypes Using Simple Sequence Repeat Markers

  • Asekova, Sovetgul;Kulkarni, Krishnanand P.;Oh, Ki Won;Lee, Myung-Hee;Oh, Eunyoung;Kim, Jung-In;Yeo, Un-Sang;Pae, Suk-Bok;Ha, Tae Joung;Kim, Sung Up
    • Plant Breeding and Biotechnology
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    • v.6 no.4
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    • pp.321-336
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    • 2018
  • Sesame (Sesamum indicum L.) is an important oilseed crop grown in tropical and subtropical areas. The objective of this study was to investigate the genetic relationships among 129 sesame landraces and cultivars using simple sequence repeat (SSR) markers. Out of 70 SSRs, 23 were found to be informative and produced 157 alleles. The number of alleles per locus ranged from 3 - 14, whereas polymorphic information content ranged from 0.33 - 0.86. A distance-based phylogenetic analysis revealed two major and six minor clusters. The population structure analysis using a Bayesian model-based program in STRUCTURE 2.3.4 divided 129 sesame accessions into three major populations (K = 3). Based on pairwise comparison estimates, Pop1 was observed to be genetically close to Pop2 with $F_{ST}$ value of 0.15, while Pop2 and Pop3 were genetically closest with $F_{ST}$ value of 0.08. Analysis of molecular variance revealed a high percentage of variability among individuals within populations (85.84%) than among the populations (14.16%). Similarly, a high variance was observed among the individuals within the country of origins (90.45%) than between the countries of origins. The grouping of genotypes in clusters was not related to their geographic origin indicating considerable gene flow among sesame genotypes across the selected geographic regions. The SSR markers used in the present study were able to distinguish closely linked sesame genotypes, thereby showing their usefulness in assessing the potentially important source of genetic variation. These markers can be used for future sesame varietal classification, conservation, and other breeding purposes.