• Journal of Microbiology and Biotechnology
• /
• v.26 no.6
• /
• pp.1067-1076
• /
• 2016

The gene encoding lipase (Lip98) from Aeromicrobium sp. SCSIO 25071 was cloned and functionally expressed in Escherichia coli. Lip98 amino acid sequence shares the highest (49%) identity to Rhodococcus jostii RHA1 lipase and contains a novel motif (GHSEG), which is different from other clusters in the lipase superfamily. The recombinant lipase was purified to homogeneity with Ni-NTA affinity chromatography. Lip98 showed an apparent molecular mass of 30 kDa on SDS gel. The optimal temperature and pH value for enzymatic activity were recorded at 30℃ and 7.5, respectively. Lip98 exhibited high activity at low temperatures with 35% maximum activity at 0℃ and good stability at temperatures below 35℃. Its calculated activation energy was 4.12 kcal/mol at the low temperature range of 15-30℃. Its activity was slightly affected by some metal ions such as K⁺, Ca²⁺, and Na⁺. The activity of Lip98 was increased by various organic solvents such as DMSO, ethanol, acetone, and hexane with the concentration of 30% (v/v) and retained more than 30% residual activity in neat organic solvent. The unique characteristics of Lip98 imply that it is a promising candidate for industrial application as a nonaqueous biocatalyst and food additive.

• Proceedings of KOSOMES biannual meeting
• /
• 2017.04a
• /
• pp.141-141
• /
• 2017

• Ocean Systems Engineering
• /
• v.5 no.4
• /
• pp.261-277
• /
• 2015

Internal solitary waves occur due to density stratification and are nonlinear in nature. These waves have been observed in many parts of the world including the South China Sea, Andaman Sea and Sulu Sea. Their effect on floating systems has been an emerging field of interest and recent offshore developments in the South China Sea where several offshore oil and gas discoveries are located have confirmed adverse effects including large platform motions and riser system damage. A valid numerical model conforming to the physics of internal waves is implemented in this paper and the effect on a spar platform is studied. The physics of internal waves is modeled by the Korteweg-de Vries (KdV) equation, which has a general solution involving Jacobian elliptical functions. The effects of vertical density stratification are captured by solving the Taylor Goldstein equation. Fully coupled time domain analyses are conducted to estimate the effect of internal waves on a typical truss spar, which is configured to South China Sea development requirements and environmental conditions. The hull, moorings and risers are considered as an integrated system and the platform global motions are analyzed. The study could be useful for future guidance and development of offshore systems in the South China Sea and other areas where the internal wave phenomenon is prominent.

• Strategy21
• /
• s.37
• /
• pp.144-176
• /
• 2015

In September 2013, President Park Geun-hye announced her controversial "Look North" policy, of which the most salient aspect is the "Eurasia Initiative". This comprises various proposals designed to overcome existing constraints by developing new markets and creating new economic partners in continental areas from which South Korea has been alienated since the end of World War II, and this dovetails nicely with China's One Belt, One Road Initiative. The concepts of the "Silk Road Rail Express (SRX)" and the "East Sea Rim Maritime Community (ESRMC)" have also been discussed. SRX is at present a purely symbolic railroad project intended to encourage individual, cultural, trade and diplomatic exchanges. ESRMC is a model for establishing an ad hoc community to promote regional economic cooperation around the East Sea. President Park's Eurasia Initiative will provide South Korean investment for the Northeast to complement Russian plans, like the "Northern Energy Road" being built by Gazprom, and Chinese plans, like the Chang-Ji-Tu Development Plan for the North Korean port of Rajin. China's trade, as well as its energy and food supplies, pass through the Strait of Malacca and the Indian Ocean, and are thus vulnerable to interdiction by India or the US. China is therefore trying to reduce its exposure geopolitical risk by establishing a network of corridors between the Belt and the Road to provide alternative paths. The "China-Pakistan Economic Corridor" and the "China-Myanmar Economic Corridor" provide such connections, and South Korea hopes that SRX and ESRMC can become part of a "China-South Korea Economic Corridor". This concept could do much to revitalize the underdeveloped northern provinces of China and Russia's Far East, not to mention North Korea. By linking up the Trans-Siberian Railway, the Trans-China Railway, the Trans-Mongolian Railway and the Trans-Korean Railway all these Asian countries will be connected to one another, and ultimately to Europe. An interim connection between China and South Korea using a rail-ferry has also been proposed.

• Ocean and Polar Research
• /
• v.34 no.2
• /
• pp.219-228
• /
• 2012

Tintinnid species distribution has been monitored in the northern East China Sea (ECS) in the summer of 2006 through 2011. This is used to understand the water mass movements in the northern ECS. The warm oceanic tintinnid species had largely spread in 2007 in the area, indicating that there was greater warm water extension into the northern ECS. However the extension of neritic water within the Changjiang diluted water mass has strengthened in 2008 and 2010 because the neritic species distribution had relatively grown in both years. These annual results based on the biological indicators of tintinnid species are well matched with the salinity change in the area. The warm oceanic species, Dadayiella ganymedes had frequently occurred over the study years and had shown a significant relationship with the salinity change. This is valuable as a key stone species for monitoring the intrusion of the Kuroshio within the northern ECS. Information from tintinnid biological indicators can support physical oceanography data to confirm ambiguous water mass properties.

• Proceedings of the Korean Environmental Sciences Society Conference
• /
• 2006.05a
• /
• pp.264-266
• /
• 2006

• Ocean Science Journal
• /
• v.41 no.2
• /
• pp.97-104
• /
• 2006

A useful radiance-converting method was developed to convert the Landsat-7 ETM+thermal-infrared (TIR) band's radiance ($L_{{\lambda},L7/ETM+}$) to that of Landsat-5 TM TIR ($L_{{\lambda},L5/TM+})$ as: $L_{{\lambda},L5/TM}=0.9699{\times}L_{{\lambda},L7/ETM+}+0.1074\;(R^2=1)$. In addition, based on the radiance-converting equation and the linear relation between digital number (DN) and at-satellite radiance, a DN-converting equation can be established to convert DN value of the TIR band between Landsat-5 and Landsat-7. Via this method, it is easy to integrate Landsat-5 and Landsat-7 TIR data to retrieve the sea surface temperature (SST) in coastal waters on the basis of local empirical algorithms in which the radiance or DN of Lansat-5 and 7 TIR band is usually the only input independent variable. The method was employed in a local empirical algorithm in Daya Bay, China, to detect the thermal pollution of cooling water discharge from the Daya Bay nuclear power station (DNPS). This work demonstrates that radiance conversion is an effective approach to integration of Landsat-5 and Landsat-7 data in the process of a SST retrieval which is based on local empirical algorithms.

• Strategy21
• /
• s.38
• /
• pp.287-320
• /
• 2015

On October 27, 2015, USS Lassen(DDG82), a 9,200 ton class Aegis destroyer of the United States Navy, began its operations within 12 nautical miles of Subi Reef, one of the seven artificial islands that China has built and claimed sovereignty over. The maneuver was joined by anti-submarine patrol airplanes such as P-8A and P-3. The White House press secretary mentioned that the President of the United States approved the operation. In response, China announced that it warned the US Navy ship about the 'illegal violation' by sending two destroyers(PLAN Lanzhou and Taizhou). This event represents a close call case where tension between the United States and China in the South China Sea might have been elevated to a conflict between the two navies. Moreover, considering that this happened only one month after Chinese president Xi's state visit to the United States, the event shows that the positions of the two countries have become starkly different to the extent that they are so hard to be reconciled. The United States' position is different from those of Vietnam and the Philippines. Countries like Vietnam and the Philippines have been directly involved in disputes with regard to sovereignty claims across the waters in the South China sea. As for the United States, being a third party in the disputes, it still cannot be a by-stander watching the whole waters in the region fall under the influence of China. Accordingly, the United States maintains that all countries bear the rights of innocent passage and military operations in the Exclusive Economic Zones(EEZ) as stipulated by the United Nations Convention on the Law of the Sea(UNCLOS). In contrast, China claims that, historically, the South China sea has been part of China's territorial waters, and that foreign countries are not allowed to conduct military operations within the waters. It strongly accuses that such military operations are illegal. Against this background, this paper tracks the different positions of the United States and China on the issues regarding the South China sea. It also carefully looks at the possibility that, in the process of dealing with the issues, the two countries may get into an armed conflict as the phrase 'Thucydides Trap' predicts.

• Maritime Security
• /
• v.1 no.1
• /
• pp.273-310
• /
• 2020

This study aims at empirically analyzing the overall mechanism of the "Gray Zone Strategy", which has begun to be used as one of Chinese major maritime security strategies in maritime conflicts surrounding the South China Sea and East China Sea since early 2010, and comparing the resulting conflict patterns in those reg ions. To this end, I made the following two hypotheses about Chinese gray zone strategy. The hypotheses that I have argued in this study are the first, "The marine gray zone strategy used by China shows different structures of implementation in the South China Sea and the East China Sea, which are major conflict areas.", the second, "Therefore, the patterns of disputes in the South China Sea and the East China Sea also show a difference." In order to examine this, I will classify Chinese gray zone strategy mechanisms multi-dimensionally in large order, 1) conflict trends and frequency of strategy execution, 2) types and strengths of strategy, 3) actors of strategy execution, and 4) response methods of counterparts. So, I tried to collect data related to this based on quantitative modeling to test these. After that, about 10 years of data pertaining to this topic were processed, and a research model was designed with a new categorization and operational definition of gray zone strategies. Based on this, I was able to successfully test all the hypotheses by successfully comparing the comprehensive mechanisms of the gray zone strategy used by China and the conflict patterns between the South China Sea and the East China Sea. In the conclusion, the verified results were rementioned with emphasizing the need to overcome the security vulnerabilities in East Asia that could be caused by China's marine gray zone strategy. This study, which has never been attempted so far, is of great significance in that it clarified the intrinsic structure in which China's gray zone strategy was implemented using empirical case studies, and the correlation between this and maritime conflict patterns was investigated.

• Journal of Environmental Science International
• /
• v.24 no.6
• /
• pp.827-840
• /
• 2015

This study analyzed a correlation between South China Sea summer (June to September) monsoon (SCSSM) and the ENSO for the last 32 years (1979 to 2010). There was a correlation that the higher (lower) the SST in the $Ni{\tilde{n}}o-3.4$ region was, the weaker (stronger) the SCSSM intensity was. To identify the reason for this correlation, a difference of means between 8 El $Ni{\tilde{n}}o$ years and 8 La $Ni{\tilde{n}}a$ years (June to September). The analysis on the difference between two groups with respect to the 850 hPa stream flows showed that there were anomalous huge cyclones in the subtropical Pacific in the both hemispheres so that cold and dry anomalous northerlies were strengthened in the South China Sea relatively while anomalous westerlies were strengthened from the Maritime Continent to the off the coast of Chile. The analysis on the difference between two groups with respect to the 200 hPa stream flows showed that the opposite anomalous pressure system pattern to that in the 850 hPa stream flows were shown. In the subtropical Pacific of the both hemispheres, anomalous anticyclones existed so that anomalous easterlies were strengthened from the Maritime Continent to the equatorial central Pacific. Considering the anomalous atmospheric circulations in the upper and lower layers of the troposphere, upward airflows from the equatorial central and eastern Pacific were downward in the South China Sea and the Maritime Continent, which was a structure of anomalous atmospheric circulations. This means that the Walker Circulation was weakened and it was a typical structure of atmospheric circulations revealed in El $Ni{\tilde{n}}o$ years.