• Journal of Microbiology and Biotechnology
• /
• 제26권6호
• /
• pp.1067-1076
• /
• 2016

The gene encoding lipase (Lip98) from Aeromicrobium sp. SCSIO 25071 was cloned and functionally expressed in Escherichia coli. Lip98 amino acid sequence shares the highest (49%) identity to Rhodococcus jostii RHA1 lipase and contains a novel motif (GHSEG), which is different from other clusters in the lipase superfamily. The recombinant lipase was purified to homogeneity with Ni-NTA affinity chromatography. Lip98 showed an apparent molecular mass of 30 kDa on SDS gel. The optimal temperature and pH value for enzymatic activity were recorded at 30℃ and 7.5, respectively. Lip98 exhibited high activity at low temperatures with 35% maximum activity at 0℃ and good stability at temperatures below 35℃. Its calculated activation energy was 4.12 kcal/mol at the low temperature range of 15-30℃. Its activity was slightly affected by some metal ions such as K⁺, Ca²⁺, and Na⁺. The activity of Lip98 was increased by various organic solvents such as DMSO, ethanol, acetone, and hexane with the concentration of 30% (v/v) and retained more than 30% residual activity in neat organic solvent. The unique characteristics of Lip98 imply that it is a promising candidate for industrial application as a nonaqueous biocatalyst and food additive.

• 해양환경안전학회:학술대회논문집
• /
• 해양환경안전학회 2017년도 공동학술발표회
• /
• pp.141-141
• /
• 2017

• Ocean Systems Engineering
• /
• 제5권4호
• /
• pp.261-277
• /
• 2015

Internal solitary waves occur due to density stratification and are nonlinear in nature. These waves have been observed in many parts of the world including the South China Sea, Andaman Sea and Sulu Sea. Their effect on floating systems has been an emerging field of interest and recent offshore developments in the South China Sea where several offshore oil and gas discoveries are located have confirmed adverse effects including large platform motions and riser system damage. A valid numerical model conforming to the physics of internal waves is implemented in this paper and the effect on a spar platform is studied. The physics of internal waves is modeled by the Korteweg-de Vries (KdV) equation, which has a general solution involving Jacobian elliptical functions. The effects of vertical density stratification are captured by solving the Taylor Goldstein equation. Fully coupled time domain analyses are conducted to estimate the effect of internal waves on a typical truss spar, which is configured to South China Sea development requirements and environmental conditions. The hull, moorings and risers are considered as an integrated system and the platform global motions are analyzed. The study could be useful for future guidance and development of offshore systems in the South China Sea and other areas where the internal wave phenomenon is prominent.

• Strategy21
• /
• 통권37호
• /
• pp.144-176
• /
• 2015

In September 2013, President Park Geun-hye announced her controversial "Look North" policy, of which the most salient aspect is the "Eurasia Initiative". This comprises various proposals designed to overcome existing constraints by developing new markets and creating new economic partners in continental areas from which South Korea has been alienated since the end of World War II, and this dovetails nicely with China's One Belt, One Road Initiative. The concepts of the "Silk Road Rail Express (SRX)" and the "East Sea Rim Maritime Community (ESRMC)" have also been discussed. SRX is at present a purely symbolic railroad project intended to encourage individual, cultural, trade and diplomatic exchanges. ESRMC is a model for establishing an ad hoc community to promote regional economic cooperation around the East Sea. President Park's Eurasia Initiative will provide South Korean investment for the Northeast to complement Russian plans, like the "Northern Energy Road" being built by Gazprom, and Chinese plans, like the Chang-Ji-Tu Development Plan for the North Korean port of Rajin. China's trade, as well as its energy and food supplies, pass through the Strait of Malacca and the Indian Ocean, and are thus vulnerable to interdiction by India or the US. China is therefore trying to reduce its exposure geopolitical risk by establishing a network of corridors between the Belt and the Road to provide alternative paths. The "China-Pakistan Economic Corridor" and the "China-Myanmar Economic Corridor" provide such connections, and South Korea hopes that SRX and ESRMC can become part of a "China-South Korea Economic Corridor". This concept could do much to revitalize the underdeveloped northern provinces of China and Russia's Far East, not to mention North Korea. By linking up the Trans-Siberian Railway, the Trans-China Railway, the Trans-Mongolian Railway and the Trans-Korean Railway all these Asian countries will be connected to one another, and ultimately to Europe. An interim connection between China and South Korea using a rail-ferry has also been proposed.

• Ocean and Polar Research
• /
• 제34권2호
• /
• pp.219-228
• /
• 2012

Tintinnid species distribution has been monitored in the northern East China Sea (ECS) in the summer of 2006 through 2011. This is used to understand the water mass movements in the northern ECS. The warm oceanic tintinnid species had largely spread in 2007 in the area, indicating that there was greater warm water extension into the northern ECS. However the extension of neritic water within the Changjiang diluted water mass has strengthened in 2008 and 2010 because the neritic species distribution had relatively grown in both years. These annual results based on the biological indicators of tintinnid species are well matched with the salinity change in the area. The warm oceanic species, Dadayiella ganymedes had frequently occurred over the study years and had shown a significant relationship with the salinity change. This is valuable as a key stone species for monitoring the intrusion of the Kuroshio within the northern ECS. Information from tintinnid biological indicators can support physical oceanography data to confirm ambiguous water mass properties.

• 한국환경과학회:학술대회논문집
• /
• 한국환경과학회 2006년도 학술발표회 발표논문집
• /
• pp.264-266
• /
• 2006

• Ocean Science Journal
• /
• 제41권2호
• /
• pp.97-104
• /
• 2006

A useful radiance-converting method was developed to convert the Landsat-7 ETM+thermal-infrared (TIR) band's radiance ($L_{{\lambda},L7/ETM+}$) to that of Landsat-5 TM TIR ($L_{{\lambda},L5/TM+})$ as: $L_{{\lambda},L5/TM}=0.9699{\times}L_{{\lambda},L7/ETM+}+0.1074\;(R^2=1)$. In addition, based on the radiance-converting equation and the linear relation between digital number (DN) and at-satellite radiance, a DN-converting equation can be established to convert DN value of the TIR band between Landsat-5 and Landsat-7. Via this method, it is easy to integrate Landsat-5 and Landsat-7 TIR data to retrieve the sea surface temperature (SST) in coastal waters on the basis of local empirical algorithms in which the radiance or DN of Lansat-5 and 7 TIR band is usually the only input independent variable. The method was employed in a local empirical algorithm in Daya Bay, China, to detect the thermal pollution of cooling water discharge from the Daya Bay nuclear power station (DNPS). This work demonstrates that radiance conversion is an effective approach to integration of Landsat-5 and Landsat-7 data in the process of a SST retrieval which is based on local empirical algorithms.

• Strategy21
• /
• 통권38호
• /
• pp.287-320
• /
• 2015

On October 27, 2015, USS Lassen(DDG82), a 9,200 ton class Aegis destroyer of the United States Navy, began its operations within 12 nautical miles of Subi Reef, one of the seven artificial islands that China has built and claimed sovereignty over. The maneuver was joined by anti-submarine patrol airplanes such as P-8A and P-3. The White House press secretary mentioned that the President of the United States approved the operation. In response, China announced that it warned the US Navy ship about the 'illegal violation' by sending two destroyers(PLAN Lanzhou and Taizhou). This event represents a close call case where tension between the United States and China in the South China Sea might have been elevated to a conflict between the two navies. Moreover, considering that this happened only one month after Chinese president Xi's state visit to the United States, the event shows that the positions of the two countries have become starkly different to the extent that they are so hard to be reconciled. The United States' position is different from those of Vietnam and the Philippines. Countries like Vietnam and the Philippines have been directly involved in disputes with regard to sovereignty claims across the waters in the South China sea. As for the United States, being a third party in the disputes, it still cannot be a by-stander watching the whole waters in the region fall under the influence of China. Accordingly, the United States maintains that all countries bear the rights of innocent passage and military operations in the Exclusive Economic Zones(EEZ) as stipulated by the United Nations Convention on the Law of the Sea(UNCLOS). In contrast, China claims that, historically, the South China sea has been part of China's territorial waters, and that foreign countries are not allowed to conduct military operations within the waters. It strongly accuses that such military operations are illegal. Against this background, this paper tracks the different positions of the United States and China on the issues regarding the South China sea. It also carefully looks at the possibility that, in the process of dealing with the issues, the two countries may get into an armed conflict as the phrase 'Thucydides Trap' predicts.

• 해양안보
• /
• 제1권1호
• /
• pp.273-310
• /
• 2020

본 연구는 최근 남중국해와 동중국해를 둘러싼 해양 갈등에서 중국의 주요 해양안보전략 중 하나로 사용되기 시작한 "회색지대전략"의 전반적인 메커니즘을 경험적으로 분석하고, 그로 인한 남중국해 및 동중국해 분쟁 양상을 비교하는 데에 그 목적을 둔다. 이를 위해 필자는 중국의 회색지대전략에 관한 두 가지 가설들을 세워 남중국해와 동중국해 분쟁에서 중국의 회색지대전략이 어떻게 '구사'되어 왔고 '구성'되어 있는지, 상대국은 어떤 방식으로 그것에 대응하는지 살펴보고, 그로 말미암아 각 분쟁들의 양상은 어떤 유사점과 차이점을 보이는지 밝혀냈다. 필자가 이 연구에서 수립한 가설들은 첫 째, "중국이 활용하는 해양 회색지대전략은 주요 분쟁 지역인 남중국해와 동중국해에서 각기 다른 수행 구조를 보인다는 것"이다. 두 번째는, "이에 따라 남중국해와 동중국해에서 벌어지는 분쟁의 양상 역시 차이를 보인다는 것"이다. 이를 논증하기 위해 필자는 중국의 회색지대전략 메커니즘을 크게 차례대로 1) 분쟁 추이와 전략 수행의 빈도, 2) 전략의 유형과 강도, 3) 전략 수행의 행위자, 4) 상대국의 대응 방식으로 다차원적인 구분을 시도하고, 양적 모델링을 바탕으로 이에 관련 데이터를 수집했다. 이후, 2010년부터 2020년 06월에 이르는 약 10년 가량의 데이터(중국의 회색지대전략 사용과 분쟁 동향 등)를 가공하고, 직접 연구 모형을 설계해 회색지대전략에 대한 새로운 범주화와 조작적 정의를 시도했다. 이를 바탕으로 필자는 중국이 활용하는 회색지대전략의 포괄적인 메커니즘들과 남중국해와 동중국해의 분쟁 양상도 성공적으로 비교해 최종적으로 모든 가설을 검증할 수 있었다. 결론 부분에서는 검증된 결과를 재차 정리하고, 중국의 회색지대전략으로 인해 비롯될 수 있는 동아시아 역내의 안보 취약성을 극복해 나가야 함을 강조하는 것으로 마무리했다. 본 연구는 지금까지 한 번도 시도되지 않았던 연구로서 중국의 회색지대전략이 수행되는 구조를 밝히고, 이것과 해양 분쟁 양상 간의 상관관계를 양적 방법론을 이용해 규명했다는 데에 큰 의의가 있으리라 생각된다.

• 한국환경과학회지
• /
• 제24권6호
• /
• pp.827-840
• /
• 2015

This study analyzed a correlation between South China Sea summer (June to September) monsoon (SCSSM) and the ENSO for the last 32 years (1979 to 2010). There was a correlation that the higher (lower) the SST in the $Ni{\tilde{n}}o-3.4$ region was, the weaker (stronger) the SCSSM intensity was. To identify the reason for this correlation, a difference of means between 8 El $Ni{\tilde{n}}o$ years and 8 La $Ni{\tilde{n}}a$ years (June to September). The analysis on the difference between two groups with respect to the 850 hPa stream flows showed that there were anomalous huge cyclones in the subtropical Pacific in the both hemispheres so that cold and dry anomalous northerlies were strengthened in the South China Sea relatively while anomalous westerlies were strengthened from the Maritime Continent to the off the coast of Chile. The analysis on the difference between two groups with respect to the 200 hPa stream flows showed that the opposite anomalous pressure system pattern to that in the 850 hPa stream flows were shown. In the subtropical Pacific of the both hemispheres, anomalous anticyclones existed so that anomalous easterlies were strengthened from the Maritime Continent to the equatorial central Pacific. Considering the anomalous atmospheric circulations in the upper and lower layers of the troposphere, upward airflows from the equatorial central and eastern Pacific were downward in the South China Sea and the Maritime Continent, which was a structure of anomalous atmospheric circulations. This means that the Walker Circulation was weakened and it was a typical structure of atmospheric circulations revealed in El $Ni{\tilde{n}}o$ years.