• Nutritional Sciences
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• 제3권1호
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• pp.11-17
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• 2000

This study was intended to examine whether dehydroepiandrosterone (DHEA) and dietary fat level or source could modulate glutathione utilizing detoxifying system activity and the cytosolic NADPH generation in rat liver. Male Sprague-Dawley rats were fed semipurifed diet containing either 2%(w/w) corn oil (low level of corn oil diet: 5 ca% of fat) 15% corn oil (high level of corn oil diet: 31 cal% of fat) or 13% sardine oil plus 2% corn oil(high level of fish oil diet: 31 cal% of fat) for 9 weeks. Half of the rats in each diet group were fed a diet supplemented with 0.2% DHEA (w/w). DHEA administration increased plasma total cholesterol level in low corn oil diet-fed rats. The high fish oil diet significantly decreased plasma total cholesterol level compared to the high corn oil diet. Plasma triglyceride level was not significantly changed by DHEA administration and dietary fat level and source. Fasting plasma glucose level was increased by DHEA administration and fish oil diet. Glucose 6-phosphate dehydrogenase activity in liver tissue was significantly increased by DHEA administration and high fat diet, especially fish oil diet. Malic enzyme activity in liver tissue was significantly increased by DHEA administration and high fat diet, especially fish oil diet. Malic enzyme activity in liver tissue was significantly increased by DHEA administration. DHEA suppressed the glutathione peroxidase, glutathione-dependent enzymes compared to the low corn oil diet, while fish oil diet elevated the activity of glutathione peroxidase and glutathione reductase compared to corn oil diet. These results suggest that DHEA administration and high level of corn oil diet may suppress the cellular detoxifying system activity through reduction of glutathione utilization, while the fish oil diet did not show these effects.

• 한국축산식품학회지
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• 제43권1호
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• pp.46-60
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• 2023

Slaughterhouse blood is a by-product of animal slaughter that can be a good source of animal protein. This research purposed to examine the functional qualities of the blood plasma from Hanwoo cattle, black goat, and their hydrolysates. Part of the plasma was hydrolyzed with proteolytic enzymes (Bacillus protease, papain, thermolysin, elastase, and α-chymotrypsin) to yield bioactive peptides under optimum conditions. The levels of hydrolysates were evaluated by 15% sodium dodecyl sulfate polyacrylamide gel electrophoresis. The antioxidant, metal-chelating, and angiotensin I-converting enzyme (ACE) inhibitory properties of intact blood plasma and selected hydrolysates were investigated. Accordingly, two plasma hydrolysates by protease (pH 6.5/55℃/3 h) and thermolysin (pH 7.5/37℃/3-6 h) were selected for analysis of their functional properties. In the oil model system, only goat blood plasma had lower levels of thiobarbituric acid reactive substances than the control. The diphenyl picrylhydrazyl radical scavenging activity was higher in cattle and goat plasma than in proteolytic hydrolysates. Ironchelating activities increased after proteolytic degradation except for protease-treated cattle blood. Copper-chelating activity was excellent in all test samples except for the original bovine plasma. As for ACE inhibition, only non-hydrolyzed goat plasma and its hydrolysates by thermolysin showed ACE inhibitory activity (9.86±5.03% and 21.77±3.74%). In conclusion, goat plasma without hydrolyzation and its hydrolysates can be a good source of bioactive compounds with functional characteristics, whereas cattle plasma has a relatively low value. Further studies on the molecular structure of these compounds are needed with more suitable enzyme combinations.

• 한국의학물리학회지:의학물리
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• 제10권1호
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• pp.1-7
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• 1999

본 연구에서는 $\beta$선 방출 동위원소들 중에서 $^{32}$P으로부터 방출되는 $\beta$선에 의한 홉수선량 분포를 방사선원의 형태와 기하학적인 조건을 달리하여 컴퓨터를 이용한 모사실험을 통해 예측함으로써 balloon catheter 및 radioactive stent의 이용과 관련된 정보를 얻고자한다. $^{32}$p로부터 방출된 $\beta$선이 인체내에서 에너지를 전달하는 과정에 대한 모사실험은 EGS4 code system 을 이용하여 수행되었다. 인체내의 방사선 흡수선량은 선원의 형태와 위치를 고려하여 축방향과 반경방향으로 등간격으로 나누어 각 격자에서 계산되었다. $^{32}$P 에서 방출되는 $\beta$선 에너지는 Coulomb 포텐셜에 대한 Dirac방정식의 해를 이용하여 계산된 $\beta$선 스펙트럼의 결과를 사용하여 무작위로 선정되었다. 체적 선원과 표면선원에서 시료 표면으로부터 반경방향으로 깊이 0.5 mm내에 있는 표적체에서의 선량률은 각각 12.133 cGy/s per GBq (0.449 cGy/s per mCi, uncertainty: 1.51%)와 24.732 cGy/s per GBq (0.915 cGy/s per mCi, uncertainty: 1.01 %)이다. 선량률은 시료표면으로부터 축방향과 반경방향으로의 거리에 따라 감소한다. 본 연구 결과를 근거로하여 balloon catheter 및 radioactive stent에 $^{32}$P 핵종을 사용할 때 치료선량을 20 Gy로 할 경우 치료에 적합한 초기 방사능량은 각각 29.69 mCi(치료시간을 3분으로 제한할 때) 와 1.2278 $\mu$Ci (영구삽입)로 계산되었다. 또한 원통형 체적선원과 표면선원에 대하여 초기방사능의 크기를 1 mCi/ml의 방사능 체적 밀도와 0.1 mCi/$cm^2$의 방사능 면 밀도로 나타내었을 때 각 표적체에서의 흡수선량률을 계산하였다. 통일한 값의 방사능 체적 밀도와 방사능 면 밀도는 크기가 다른 모델에 대해서 비슷한 크기의 홉수선량을 유도하므로 $^{32}$p 방사선원의 초기 방사능 체적 밀도와 초기 방사능 면 밀도를 알고 있을 때 본 연구의 계산 결과를 이용하면 직경과 길이가 다른 $^{32}$P 핵종의 원통형 모델 주위의 홉수선량 분포를 쉽게 계산할 수 있다.

• 펄프종이기술
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• 제31권3호
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• pp.68-76
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• 1999

Coprinus cinereus 2249 producing alkaline-active cellulase was screened from 29 species of Corpinaceae and constitutively produced alkaline carboxymethyl cellulase (CMCase) and filter paper cellulase (Fpase). When cultivated at pH 9.0, 25$^{\circ}C$ and 5 days, copnnus cinereus 2249 produced higher alkaline activity on 0.5% CMC, 2% wheat bran as carbon source and 0.5% peptone, 0.05% yeast extract as nitrongen source compared with other culture conditions. The level of cellulase production was higher in the presence of wheat bran than in the presence of CMC. The optimum temperature and pH for alkaline -active cellulase activity weas 50$^{\circ}C$ and 9, 0, respectively.

• 한국미생물생명공학회:학술대회논문집
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• 한국미생물생명공학회 1978년도 추계학술대회
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• pp.207.5-208
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• 1978

Effects of the plant growth regulator (P. G. R.)on the reaction of proteinase, $\gamma-amylase$ and acid phosphatase were investigated, and also were the conditions of production of P. G. R. by Stroptomyces sp. 445. The P. G. R. had no effect on the act ivities of such enzymes in mung bean seedling. But in germinating seed previously treated with P. G. R. it effected the activity of protease in cotyledon. In the conditions of production of P. G. R., the maxim, activity was appeared in shaking cutlure at $30^{\circ}C$ for 5 days, and by the addition of peptone or casein hydrolysate as nitrogen source, soluble starch as carbon source, and sulfur as metal ion.

• 한국미생물·생명공학회지
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• 제27권5호
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• pp.370-377
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• 1999

Thermo-tolerant bacterium producing the xylanase was isolated from soil and identified as Bacillus pumilus. This strain, named Bacillus pumilus TX703, was able to grow ad produce xylanase at the culture temperature of 5$0^{\circ}C$. The maximum xylanase production was obtained when 1%(w/v) birchwood xylan and 1% (w/v) soytone were used as carbon source and nitrogen source, respectively. The biosynthesis of xylanase was under the catabolite repression induced by glucose in the culture medium, and it was completely inhibited in the presence of 0.2% (w/v) glucose. The maximum activity of xylanase was observed from pH8.0 to 9.0 and from 50 to 6$0^{\circ}C$ and the enzyme was highly heat-stable, whose activity remained was over 50% at 8$0^{\circ}C$, and was quite stable from pH5.0 to 10.0.

• 통상정보연구
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• 제10권4호
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• pp.81-96
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• 2008

uEFL is an open source solution to integrate general business/learning activities and processes. uEFL is originally developed to adopt LD (Learning Design) specification, which represents learning as various combination of learning activities with learning conditions and outcomes. Learning activities are described with participant's role, learning environment, and contextual sequence. This viewpoint resembles BPM (Business Process Modeling). uEFL can convert LD to BPM description. uEFL engine can run converted LD activity with other business activities. This paper presents 4 templates and 2 sample models for uEFL. The templates and models will show how learning activities can be integrated with business activities efficiently.

• 한국식품영양학회지
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• 제5권2호
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• pp.137-143
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• 1992

As a research for developing biofertilizers, Klebsiella oxytoca, an associative nitrogen fixer in the rhizosphere of rice plant in the soil of paddy field, was subjected to molecular breeding. The results obtained were as followings. 1). By transforming pbIC71A, Nif A overproducing plasmid, into Klebsiella oxytoca NGl3, Klebsiell6f oxytoca SH3l, and Klebsiella oxytoca SH161, nitrogenase activities in the absence of nitrogen source in the medium were increased 6.4, 17.2, and 13.5 times, respectively, in comparison with the parent strains. 2). Nitrogenase activity of Klebgiella oxytoca NGl3, Klebsiella oxytoca SH3l, and Klebsiella oxytoca SH161 was completely repressed In the presence of 15mM NH4+. But, nitrogenase activities of Klebsiella oxytoca NGl3/PMC71A, Klebsiella oxytoca SH3l /PMC71A, and Klebsiella oxytoca SH 161/pMC714 harboring PMC71A, were 13.7%, 7.7%, and 6.2% of the nitrogenase activities in the absence of nitrogen source in the medium, respectively.

• 한국식품영양학회지
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• 제6권3호
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• pp.199-207
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• 1993

In order to obtain a good microorganism capable of degrading microcrystaline cellulose (avicel), the screening test was carried out from soil and brown-rot wood. 8 strains which had good avicel-hydrolyzing activity were isolated. Among them, HK 47 which exhibited the highest avicel hydrolyzing activity was identified as Trichoderma sp. HK 47. Maximum avicel-hydrolyzing enzyme production from Trichoderma sp. HK 47 was obtained with the optimum medium contained carboxymethylcellulose 1.5% as carbon source, NaN030.75% as nitrogen source, KH2P040.5%, MgSO4.7H2O 0.1%, Tween 800.005% (V/V) during stationary cultivation at pH 6.0, 3$0^{\circ}C$ In this case, the production of avicel-hydrolyzing enzyme was 0.028 U/ml.

• ETRI Journal
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• 제42권6호
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• pp.932-942
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• 2020

To recover the neural activity from Magnetoencephalography (MEG) and Electroencephalography (EEG) measurements, we need to solve the inverse problem by utilizing the relation between dipole sources and the data generated by dipolar sources. In this study, we propose a new approach based on the implementation of a particle filter (PF) that uses minimum sampling variance resampling methodology to track the neural dipole sources of cerebral activity. We use this approach for the EEG data and demonstrate that it can naturally estimate the sources more precisely than the traditional systematic resampling scheme in PFs.