• 한국지반환경공학회 논문집
• /
• 제10권1호
• /
• pp.15-23
• /
• 2009

본 연구의 목적은 사용종료 비위생매립지의 안정화 정도를 평가하고 안정화되지 않은 사용종료 매립지의 재정비 방안을 도출하는데 있다. 안정화 정도는 기존 사용종료 비위생매립지 21개소의 매립폐기물 시료를 채취하여 그 성상을 분석하여 평가하였다. 물리적 성상으로는 토사, 유기물, 가연성 및 불연성으로 분석하였다. 그 결과 매립폐기물 시료를 채취한 9개소의 비위생매립지는 사용종료 후 10년 이상 경과되었음에도 완전한 안정화가 이루어지지 않았으며, 사용종료 비위생매립지의 재정비가 시급함을 알 수 있었다. 비위생매립지의 물리적 성상중 토사 함량이 높았으며 가장 높은 함량은 89.96%이었다. 이와는 달리 위생매립지의 경우는 9.89~11.12%로 토사함량이 낮았다. 따라서 선별이적처리에 의해 회수된 토사는 재정비를 해야하는 비위생매립지의 복토재나 건설재료로의 재활용할 수 있을 것으로 평가되었다.

• 한국수정란이식학회지
• /
• 제26권3호
• /
• pp.181-186
• /
• 2011

Spermatozoa sorted by flow cytometry have been successfully used to produce offspring in domestic animals and are commercially available for cattle. Also sheath fluid is the important environment for viability of sex-sorted sperm in flow cytometry. The aim of this study was to investigate whether or not HEPES (N-2-hydroxyethylpiperazine-N'-2-Ethanesulfonic acid) has any effect on the viability in sex-sorted Hanwoo (Korean native cattle) sperm. In this study, the semen was collected from Hanwoo of Hoengseong Livestock Cooperation by artificial vagina method then pooled and subjected to cryopreservation in straws. Sperm were cultured for 0, 30, 60 and 120 min with 0, 2.5, 5, 7.5 and 10 mM of HEPES added to the sheath fluid and incubated at 4, 20 and 38$^{\circ}C$, respectively. For the cytometric analysis the frozen-thawed semen was extended with 5 mM HEPES extender to final concentration ($2{\times}10^7$ spermatozoa) at 4, 20 and 37$^{\circ}C$. Sperm viability was assessed with SYBR-14 and propidium iodide (PI) staining. This study shows that the viability of sperm was decreased with prolongation of incubation time in all of test. But the viability of sperm which were treated with 38$^{\circ}C$ was gently decreased than that of treated with other temperature. The viability of the control was sharply decreased (p<0.05) than all of the HEPES treatment group at 60 to 120 min in 38$^{\circ}C$. X-sexed sperm was more sensitive than Y-sexed sperm to temperature during f10w cytometry (p<0.05). In conclusion, the results of this study suggest that the sheath fluid with 5 mM HEPES has effect on maintenance of viability after sperm sexing at 37$^{\circ}C$ in Hanwoo.