• Journal of the Korean GEO-environmental Society
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• v.10 no.1
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• pp.15-23
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• 2009

The aim of this study was an evaluation of closed non-sanitary landfill's stabilization degree and a determination of its an optimal reclamation method. In order to evaluate the stabilization degree, physical compositions of landfill wastes in 21 closed non-sanitary landfills were analyzed. There were 4 major items such as cover soils, organics, combustibles and incombustibles. With respect to the results of physical compositions, it was determined that the waste in 9 sampling sites of closed non-sanitary landfills after 10years of the relief time was not fully stabilized. The closed non-sanitary landfills must be reclaimed as soon as possible. The main material in closed non-sanitary landfills was cover soils and the highest content was 89.96%. Otherwise, the contents in sanitary landfills was small and 9.89~11.12%. Therefore, it was evaluated that the recovered soil by sorting transfer treatment could be reused as on-site cover soils of the reclamating non-sanitary landfills and/or constructing materials.

• Journal of Embryo Transfer
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• v.26 no.3
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• pp.181-186
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• 2011

Spermatozoa sorted by flow cytometry have been successfully used to produce offspring in domestic animals and are commercially available for cattle. Also sheath fluid is the important environment for viability of sex-sorted sperm in flow cytometry. The aim of this study was to investigate whether or not HEPES (N-2-hydroxyethylpiperazine-N'-2-Ethanesulfonic acid) has any effect on the viability in sex-sorted Hanwoo (Korean native cattle) sperm. In this study, the semen was collected from Hanwoo of Hoengseong Livestock Cooperation by artificial vagina method then pooled and subjected to cryopreservation in straws. Sperm were cultured for 0, 30, 60 and 120 min with 0, 2.5, 5, 7.5 and 10 mM of HEPES added to the sheath fluid and incubated at 4, 20 and 38$^{\circ}C$, respectively. For the cytometric analysis the frozen-thawed semen was extended with 5 mM HEPES extender to final concentration ($2{\times}10^7$ spermatozoa) at 4, 20 and 37$^{\circ}C$. Sperm viability was assessed with SYBR-14 and propidium iodide (PI) staining. This study shows that the viability of sperm was decreased with prolongation of incubation time in all of test. But the viability of sperm which were treated with 38$^{\circ}C$ was gently decreased than that of treated with other temperature. The viability of the control was sharply decreased (p<0.05) than all of the HEPES treatment group at 60 to 120 min in 38$^{\circ}C$. X-sexed sperm was more sensitive than Y-sexed sperm to temperature during f10w cytometry (p<0.05). In conclusion, the results of this study suggest that the sheath fluid with 5 mM HEPES has effect on maintenance of viability after sperm sexing at 37$^{\circ}C$ in Hanwoo.