• Journal of the Korean Institute of Landscape Architecture
• /
• v.29 no.3
• /
• pp.29-37
• /
• 2001

The purpose of this study is to determine the suitable illuminance model of an urban neighborhood park. To this end, 1 dependent variable and 11 independent variables were set, and multiple regression analysis was applied to find correlation between variables and the model. The results of this study are as follows; 1) Among 11 independent variables abstracted to study suitable illuminance model of an urban neighborhood park, as a result of analysis on correlation between suitable illuminance of a dependent variable and activity space by using dummy variables, activity type and illuminance by spaces the suitable illuminance required for an urban neighborhood park was much influenced by activity type, and the fact that the activity was not limited to a specific space. 2) As a result of multiple regression analysis, independent variables influencing the suitable illuminance model of an urban neighborhood park were activity space, illuminance by spaces, seated activity, standing activity, and sporting activity. And, for relative contribution of independent variables to suitable illuminance, activity with sporting showed an importance 22 times higher than seated activity. When the central square(Sp_1) of activity spaces was set to reference category using dummy variables, it showed a contribution 52 times higher than sorting space(Sp_7) and the central square as the most important variable. 3) It was analyzed that suitable illuminance of an urban neighborhood part was most influenced by sporting activity but the relative contribution of a sporting space where activity with sporting occurs was least in view of the function of the space. Very high illuminance is required to accept high activity, and if illuminance at a certain space becomes too high, it may influence the illuminance of other spaces, and may consequently have a negative effect on the suitable illuminance of an urban neighborhood park. The results of this study were mainly for teenagers and young adults in their twenties, so further concrete studies which consider gender and a wider age range are needed in the future. Based on subsequent study, suitable illuminance by spaces shall be suggested.

• Reproductive and Developmental Biology
• /
• v.37 no.3
• /
• pp.143-148
• /
• 2013

Spermatogenesis is initiated from spermatogonial stem cells (SSCs) that has an ability of self-renewal and unipotency to generate differentiating germ cells. The objective of this study is to develop the simple method for derivation of SSCs using non-sorting of both spermatogonia and feeder cells. Simply uncapsulated mouse testes were treated with enzymes followed by surgical mincing, and single cells were cultured in stempro-$34^{TM}$ cell culture media at $37^{\circ}C$. After 5 days of culture, aciniform of SSC colony was observed, and showed a strong alkaline phosphatase activity. Molecular characterization of mouse SSCs showed that most of the mouse SSC markers such as integrin ${\alpha}6$ and ${\beta}1$, CD9 and Stra8. In addition, pluripotency embryonic stem cell (ESC) marker Oct4 were expressed, however Sox2 expression was lowered. Interestingly, expression of SSC markers such as Vasa, Dazl and PLZF were stronger than mouse ESC (mESC). This data suggest that generated mouse SSCs (mSSCs) in this study has at least similar biomarkers expression to mESC and mSSCs derived from other study. Immunocytochemistry using whole mSSC colony also confirmed that mSSCs generated from this study expressed SSC specific biomarkers such as c-kit, Thy1, Vasa and Dazl. In conclusion, mSSCs from 5 days old mouse testes were successfully established without sorting of spermatogonia, and this cells expressed both mESC and SSC specific biomarkers. This simple derivation method for mSSCs may facilitate the study of spermatogenesis.

• BMB Reports
• /
• v.35 no.4
• /
• pp.395-402
• /
• 2002

Caveolae are small, flask-shaped, non-clathrin coated invaginations of the plasma membrane of many mammalian cells. Caveolae have a coat that includes caveolin. They have been implicated in numerous cellular processes, including potocytosis. Since the human folate receptor (hFR) and other glycosyl-phosphatidylinositol (GPI)-tailed proteins have been co-localized to caveolae, we studied the caveolin role in the hFR function by transfecting hFR and/or caveolin cDNA into Fischer rat thyroid epithelial (FRT) cells that normally do not express detectable levels of either protein. We isolated and characterized stable clones as follows: they express (1) high levels of caveolin alone, (2) hFR and caveolin, or (3) hFR alone. We discovered that hFR is correctly processed, sorted, and anchored by a GPI tail to the plasma membrane in FRT cells. No difference in the total folic acid binding or cell surface folic acid binding activity were found between the FRT cells that were transfected with hFR, or cells that were transfected with hFR and caveolin. The hFR that was expressed on the cell surface of clones that were transfected with hFR was also sensitive to phosphatidylinositol-specific phospholipase C (PI-PLC) release, and incorporated radiolabeled ethanolamine that supports the attachment of a GPI-tail on hFR. We conclude that the processing, sorting, and function of hFR is independent on the caveolin expression in FRT cells.

• English Language & Literature Teaching
• /
• no.3
• /
• pp.95-122
• /
• 1997

The purpose of this research was to investigate the effects of content and formal schema on reading comprehension. Five hundred fiftynine subjects from high school were assigned to one of the following levels and treatment conditions : (1) Higher level & Schema Activation, (2) Higher level & Non-schema Activation, (3) Lower level & Schema Activation, and (4) Lower level & Non-schema Activation. To evaluate the effects of schema activation. two experiments were conducted : one was related to the content schema and the other to the formal schema. To evaluate the effects of content schema, three different types of tests were conducted : (1) cloze test, (2) guessing the meanings of nonsense words, and (3) immediate recall test. To evaluate the effects of formal schema instruction, four kinds of tests were conducted : (1) sorting the sentences according to the importance, (2) identifying the signal words, (3) immediate recall test, and (4) identifying the specific information. For content schema condition, results indicated that the subjects given the titles or pictures before reading in "Content Schema Activation" treatment had better grades than those of the other treatment in all types of tests. regardless of their levels. Schema activation helped the subjects to increase the cognitive predictability of missing words and to participate in the tasks more actively with risk-taking. And it was also shown that good readers tend to process the words meaningfully, while poor readers tend to process the words phonetically or morphologically. Formal schema activation through teaching the text organization also had a significant influence on three types of tests: sorting the sentences according to the importance, identifying the signal words, and immediate recall test, but not on identifying the specific information. The implications from this study can be briefly noted as follows : (l) In teaching reading, the student's background knowledge should be activated as a pre-reading activity. (2) In reading, it is more important to emphasize the student's schema than the features of the text. (3) Various educational interventions should be introduced, especially for the lower level students. (4) Teaching text structures can be a powerful method for the top-down processing strategy.

• Korean Journal of Microbiology
• /
• v.53 no.2
• /
• pp.71-78
• /
• 2017

Raman microspectroscopy is a promising tool for microbial analysis at single cell level since it can rapidly measure the cell materials including lipids, nucleic acids, and proteins by measuring the inelastic scattering of a molecule irradiated by monochromatic lights. Using Raman spectra provides high specificity and sensitivity in classification of bacteria at the strain level. In addition, a Raman approach coupled with stabled isotope such as $^{13}C$ and $^2H$ is able to detect and quantify general metabolic activity at single cell level. After bacterial detection process by Raman microspectroscopy, interested unculturable cell sorting and single cell genomics can be accomplished by combination with optical tweezer and microfluidic devices. In this review, the characteristics and applications of Raman microspectroscopy were reviewed and summarized in order to provide a better understanding of microbial analysis using Raman spectroscopy.

• Journal of Pharmacopuncture
• /
• v.16 no.4
• /
• pp.7-13
• /
• 2013

Objectives: Whether the ultra-highly-diluted remedies used in homeopathy can effectively bring about modulations of gene expressions through acetylation/deacetylation of histones has not been explored. Therefore, in this study, we pointedly checked if the homeopathically-diluted anti-cancer remedy Condurango 30C (ethanolic extract of Gonolobus condurango diluted $10^{-60}$ times) was capable of arresting the cell cycles in cervical cancer cells HeLa by triggering an epigenetic modification through modulation of the activity of the key enzyme histone deacetylase 2 vis-a-vis the succussed alcohol (placebo) control. Methods: We checked the activity of different signal proteins (like $p21^{WAF}$, p53, Akt, STAT3) related to deacetylation, cell growth and differentiation by western blotting and analyzed cell-cycle arrest, if any, by fluorescence activated cell sorting. After viability assays had been performed with Condurango 30C and with a placebo, the activities of histone de-acetylase (HDAC) enzymes 1 and 2 were measured colorimetrically. Results: While Condurango 30C induced cytotoxicity in HeLa cells in vitro and reduced HDAC2 activity quite strikingly, it apparently did not alter the HDAC1 enzyme; the placebo had no or negligible cytotoxicity against HeLa cells and could not alter either the HDAC 1 or 2 activity. Data on $p21^{WAF}$, p53, Akt, and STAT3 activities and a cell-cycle analysis revealed a reduction in DNA synthesis and G1-phase cell-cycle arrest when Condurango 30C was used at a 2% dose. Conclusion: Condurango 30C appeared to trigger key epigenetic events of gene modulation in effectively combating cancer cells, which the placebo was unable to do.

• Reproductive and Developmental Biology
• /
• v.29 no.2
• /
• pp.109-115
• /
• 2005

Hematopoietic stem cells (HSC) are multipotent cells that reside in the bone marrow and replenish all adult hematopoietic lineages throughoutthe lifetime. In this study, we analyzed the expression of receptors of $P2Y_{10}$, purinergic receptor families in murine hematopoietic stem cells, hematopoietic progenitor cells. In addition, the biological activity of $P2Y_{10}$ was investigated with B lymphocyte cell line, Ba/F3 in effect to cell growth and cell cycle. From the analysis of expression in hematopoieticstem cell. and progenitor with RT-PCR, $P2Y_{10}$ was strongly expressed in murine hematopoieticstem cells (c-kit+ Sca-l+ Lin-) and progenitor cell population, such as c-kit- Sca-l+ Lin-, c-kit+ Sca-l- Lin- and c-kit- Sca-l- Lin-. To investigate the biological effects by $P2Y_{10}$, retroviral vector from subcloned murine $P2Y_{10}$ cDNA was used fur gene introduction into Ba/F3 cells, and stable transfectant cells were obtained by flow cytometry sorting. In cell proliferation assay, the proliferation ability of $P2Y_{10}$ receptor gene­transfected cells was strongly inhibited, and the cell cycle was arrested at G1 phase. These result suggest that the $P2Y_{10}$ may be involved the biological activity in hematopoietic stem cells and immature B lymphocytes.

• Proceedings of the Korean Institute of Intelligent Systems Conference
• /
• 1997.11a
• /
• pp.191-194
• /
• 1997

본 논문에서는 2D-DCT 변환된 동영상 프레임 사이의 오차 블록들의 활성 레벨(atcivity level)과 에지의 특성을 분류하여 동영상의 적응적인 양자화를 제안한다. 각 블록에서는 활성 레벨이 각기 다르고, 같은 활성 레벨이라 할지라도 에지의 특성도 각기 다르게 나타난다. 적응적인 양자화를 위해서, 2D-DCT 변환된 영상 오차의 각 블록의 활성레벨 뿐만 아니라 AC 계수들의 분포에 따른 에지 특성을 분류하면, 블록의 활성 레벨만을 일률적으로 적용한 Sorting 방법의 경우보다 향상된 영상을 복원할 수 있다. 블록의 활성 레벨은 AC energy에 의해서 측정하고, 에지 특성은 AC 계수들의 분포에 의해 결정하게 된다.

• The Transactions of the Korean Institute of Electrical Engineers
• /
• v.43 no.7
• /
• pp.1193-1205
• /
• 1994

This paper presents a fuzzy system that adaptively classifies subimages to four classes according to image activity distribution. In adaptive transform image coding, subimage classification improves the compression performance by assigning different bit maps to different classes. A conventional classification method sorts subimages by their AC energy and divides them to classes with equal number of subimages. The fuzzy system provides more flexible classification to natural images with various distribution of image details than does the conventional method. Clustering of training data in the input-output product space generated the fuzzy rules for subimage classification. The fuzzy system of small number of fuzzy rules successfully classified subimages to improve the compression performance of the transform image coding without sorting of AC energies.

• BMB Reports
• /
• v.41 no.12
• /
• pp.827-832
• /
• 2008

Many age-dependent neurodegenerative diseases are associated with the accumulation of abnormally folded proteins within neurons. One of the major proteolytic pathways in the cell is the autophagy pathway, which targets cytoplasmic contents and organelles to the lysosomes for bulk degradation under various physiological and stressful conditions. Although the importance of autophagy in cellular physiology is well appreciated, its precise roles in neurodegeneration remain largely unclear. Recent studies indicate that components of the endosomal sorting complex required for transport (ESCRT) are important in the autophagy pathway. Reduced activity of some ESCRT subunits leads to the accumulation of autophagosomes and failure to clear intracellular protein aggregates. Interestingly, rare mutations in CHMP2B, an ESCRT-III subunit, are associated with frontotemporal dementia linked to chromosome 3 (FTD3). Mutant CHMP2B proteins seem to disrupt the fusion of autophagosomes and lysosomes in cell culture models. These findings suggest a potential mechanism for the pathogenesis of FTD3 and possibly other neurodegenerative diseases as well.