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Studies on electrophoretic pattern and amino acids of wild soybean protein[1] -Acrylamide gel electrophoretic pattern of seed protein- (야생대두단백질(野生大豆蛋白質)의 전기영동유형(電氣泳動類型)및 아미노산조성(酸組成)에 관(關)한 연구(硏究)[1] -종실단백질(種實蛋白質)의 Acrylamide gel 전기(電氣) 영동유형(泳動類型)-)

  • Park, H.;Lee, J.S.;Lee, C.Y.
    • Applied Biological Chemistry
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    • v.20 no.2
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    • pp.247-254
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    • 1977
  • Acrylamide gel electrophoretic pattern of 13 wild soybean (Glycine ussuriensis) was compared with that of G. gracilis and G. max var. Gwanggyo. Average Protein content (50%) of wild soybean was greater than that of C. gracilis (46%) and Gwanggyo (45%). Grain weight of wild soybean was one third of G. gracilis and one ninth of Gwanggyo. Electrophoresis of wild soybean protein showed total 16 different bands and three of which (Rm 0.09, 0.59 and 0.84) were specific and did not appeared in 86 var. of G. max which showed four specific bands (Rm 0.35, 0.45, 0.50 and 0.77) of total 17 bands. G. glacilis had all bands of Gwanggyo and two bands (Rm 0.53 and 0.59), one of which (Rm 0.59) was specific for wild soybean indicating that G. gracilis is middle type. Of 16 protein bands the third band (32%), the first band (28%) and the 5th band (13%) were main bands. Electrophoretic pattern could be sorted qualitatively into 4 groups, semiquantitatively into 6 groups and 2 or 4 groups depending on reference pattern by correlation or pattern similarity method. All sorting methods separated a wild soybean from Sogri mountain into a group and except that there were no similarity among methods but correlation methods seems more reasonable. Protein content was no relation with electrophoretic pattern but positively correlated with percent contribution of first band at 5% level suggesting that the first band may have a important role for protein synthesis.

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Environmental Characteristics of Seawater and Sediment in Mariculture Management Area in Ongjin-gun, Korea (옹진군 어장관리해역의 수질 및 퇴적물 환경 특성)

  • Kim, Sun-Young;Kim, Hyung-Chul;Lee, Won-Chan;Hwang, Dong-Woon;Hong, Sok-Jin;Kim, Jeong-Bae;Cho, Yoon-Sik;Kim, Chung-Sook
    • Journal of the Korean Society of Marine Environment & Safety
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    • v.19 no.6
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    • pp.570-581
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    • 2013
  • To improve productivity of aquaculture animals with management of culturing grounds, survey of mariculture management area in Ongjin-gun about water quality and sedimentary environment had been conducted on June, August and November in 2011. Water temperature in surface and bottom waters ranged from 9.49 to $24.14^{\circ}C$. Salinity and dissolved oxygen concentrations were in the range of 23.19~31.49 and 5.48~9.36 mg/L, respectively, depending on the variation of water temperature. The average concentration of COD was 1.57 mg/L and the concentrations of DIN and DIP showed entirely low level. As the result of grain size analysis, sand(56.66 %) and silt(34.60 %) were predominated. The Mz of sediment showed a variation of 2.59 to $6.62{\O}$ and sorting appeared to be poorly sorted. The concentrations of COD and IL in surface sediment ranged from 1.00 to $11.03mg/g{\cdot}dry$ and 0.72 to 5.29 %, respectively, which showed relatively good positive correlations. On the environmental assessment of trace metals in surface sediment, geoaccumulation index ($I_{geo}$) class indicated that sediments were not contaminated by most of metallic elements except Cr and As. Our result implies that this study area showed good water quality and sediments were not polluted by organic matters and metallic elements.

Cooling Time Determination of Spent Nuclear Fuel by Detection of Activity Ratio $^{l44}Ce /^{l37}Cs$ (방사능비 $^{l44}Ce /^{l37}Cs$ 검출에 의한 사용후핵연료 냉각기간 결정)

  • Lee, Young-Gil;Eom, Sung-Ho;Ro, Seung-Gy
    • Nuclear Engineering and Technology
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    • v.25 no.2
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    • pp.237-247
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    • 1993
  • Activity ratio of two radioactive primary fission products which had sufficiently different half-lives was expressed as functions of cooling time and irradiation histories in which average burnup, irradiation time, cycle interval time and the dominant fissile material of the spent fuel were included. The gamma-ray spectra of 36 samples from 6 spent PWR fuel assemblies irradiated in Kori unit-1 reactor were obtained by a spectrometric system equipped with a high purity germanium gamma-ray detector. Activity ratio $^{l44}$Ce $^{l37}$Cs, analyzed from each spectrum, was used for the calculation of cooling time. The results show that the radioactive fission products $^{l44}$Ce and $^{l37}$Cs are considered as useful monitors for cooling time determination because the estimated cooling time by detection of activity ratio $^{l44}$Ce $^{l37}$Cs agreed well with the operator declared cooling time within relative difference of $\pm$5 % despite the low counting rate of the gamma-ray of $^{l44}$Ce (about 10$^{-3}$ count per second). For the samples with several different irradiation histories, the determined cooling time by modeled irradiation history showed good agreement with that by known irradiation history within time difference of $\pm$0.5 year. From this result, it would be expected to be possible to estimate reliably the cooling time of spent nuclear fuel without the exact information about irradiation history. The feasibility study on identification of and/or sorting out spent nuclear fuel by applying the technique for cooling time determination was also performed and the result shows that the detection of activity ratio $^{l44}$Ce $^{l37}$Cs by gamma-ray spectrometry would be usefully applicable to certify spent nuclear fuel for the purpose of safeguards and management in a facility in which the samples dismantled or cut from spent fuel assemblies are treated, such as the post irradiation examination facility.mination facility.

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Grain-Size Trend Analysis for Identifying Net Sediment Transport Pathways: Potentials and Limitations (퇴적물 이동경로 식별을 위한 입도경향 분석법의 가능성과 한계)

  • Kim, Sung-Hwan;Rhew, Ho-Sahng;Yu, Keun-Bae
    • Journal of the Korean Geographical Society
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    • v.42 no.4
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    • pp.469-487
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    • 2007
  • Grain-Size Trend Analysis is the methodology to identify net sediment transport pathways, based on the assumption that the movement of sediment from the source to deposit leaves the identifiable spatial pattern of mean, sorting, and skewness of grain size. It can easily be implemented with low cost, so it has great potentials to contribute to geomorphological research, whereas it can also be used inadequately without recognition of its limitations. This research aims to compare three established methods of grain-size trend analysis to search for the adequate way of application, and also suggest the research tasks needed in improving this methodology 1D pathway method can corporate the field experience into analyzing the pathway, provide the useful information of depositional environments through X-distribution, and identify the long-term trend effectively. However, it has disadvantage of the dependence on subjective interpretation, and a relatively coarse temporal scale. Gao-Collins's 2D transport vector method has the objective procedure, has the capability to visualize the transport pattern in 2D format, and to identify the pattern at a finer temporal scale, whereas characteristic distance and semiquantitative filtering are controversial. Le Roux's alternative 2D transport vector method has two improvement of Gao-Collins's in that it expands the empirical rules, considers the gradient of each parameters as well as the order, and has the ability to identify the pattern at a finer temporal scale, while the basic concepts are arbitrary and complicated. The application of grain sire trend analysis requires the selection of adequate method and the design of proper sampling scheme, based on the field knowledge of researcher, the temporal scale of sediment transport pattern targeted, and information needed. Besides, the relationship between the depth of sample and representative temporal scale should be systematically investigated in improving this methodology.

Studies on the Antioxioative Character in the Etnyl Acetate Extractions of Rumex crispus (Rumex crispus의 에칠아세테이트 추출물의 항산화 성분에 관한 연구)

  • 신춘혜
    • KSBB Journal
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    • v.16 no.6
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    • pp.592-602
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    • 2001
  • This study was undertaken to investigate the antioxidative substance and activity of ethyl acetate extracted from Rumex crispus. Sample extracted follow in proper course of a solvent. Material refinement was carried out using silicagel column and Sephadex LH-20 column chromatography. Material sorting was carried by Gas Chromatography(GC/MS). 1,1-Diphenyl-2-Picrylhydrazyl(DPPH) free radical scavenging and enzyme activity were measured for antioxidative activity. as result of testing by DPPH free radical scavenging activity, Antioxidative activity was shown as the highest in the root, then leaf and stem in order. Ethyl acetate extraction of root part were 50% inhibitory concentration (IC50) Rumex activty(6.1 ug/mL). Rumex nipponicus(9.8 ug/ml) and Rumex acetoceae(31.5 ug/mL) in leaf part. The highest antioxidative activity of sample refined through silicagel column chromatography of Rumex crispus was appealed Fraction 5(IC50;3.57 ug/mL) in root and Fraction 6(IC50;85.9 ug/mL) in leaf. Fraction 5 in roof & Fraction 6 in leaf were refined using Sephadex LH-20 column chromatography. The highest antioxidative activity were appeared Fraction 4 (IC50;3.57 ug/mL) and Fraction 4 (IC50;18.41 ug/mL)in leaf. As for main phenol compounds 2,6-Dichloro-4-nitropnenol and 2-Isopropyl-5-methyl Phenol were identified in root and leaf, While 4-Vinyl-2- methoxy-phenol and 2,3-Dihydro- benzofuran were identifica ted only in leaf. Enzyme activity was shown low both in peroxidase(PDD) Non-activate(IU/mg protein)and in Superoxide dismutase(SOD) non-activate(IU/mg protein). 2,6-Dichloro-4-nitrophenol, 2-Isopropyl-5-methyl phenol, 4-Vinyl-2-methoxy-phenol were obtained in this experiment and these compounds are phenolic compounds which have OH group in the structure. With the result of this study these phenolic compounds which are extracted from Rumex crispus have high antioxidative effect. This antioxidative effect of Rumex crispus can be applied for chromo-preventive and antioxidative supplements which can be used for anti-allegy, aging, anti-tumor, aging and other oxidative disease for health promotion.

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Fluvial Deposits Distributed along the Seomjin River (섬진강 유역의 하성 퇴적층에 관한 연구)

  • You, Hoen-Su;Cho, Seok-Hee;Koh, Yeong-Koo
    • Journal of the Korean earth science society
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    • v.21 no.2
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    • pp.174-187
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    • 2000
  • The Quaternary coarse-grained sandy sediments are distributed along the channels of Seomjin River. The fluvial sediments were sedimentologically studied in horizontal and vertical distributions. To analyze depositional environments and facies changes in the sediments, sediment sampling from river mouth to upper stream and desctriptive approaches to the sediment profiles outcropped near Kurye were carried out. The sediments along the stream lines of the river are assigned to very coarse to coarse sand in grain size. The sediment grains are widely scattered in sorting and moderately sorted in average. For skewness and kurtosis, the sediments ranges from very fine to very coarse skewed and from very lepto-kurtic to extremelyl epto-kurtic states, respectively. The sediments are divided into slightly gravelly sand, gravelly sand and sandy gravel in sediment type. The pain shape in the sandy sediments are dominant in equant and tabular forms showing wide varieties. The sandy sediments are mostly poorly sorted and are highly variable in surface texture with SEM. Some smaller grains in the sediments ordinarily show polished surfaces. Of those grains, quartz ones are commonly angular to surounded. On the basis of facies changes and sedimentary structures, outcropped fluvial sediment profiles in Kurye are classified into xGyS, mGyS, gGyS, xSM, xS, mS, mGyM, IgM in facies. These eight facies are reformed as facies assemblage I and ll. The facies assemblage I and II are interpreted as the products of the channel deposits in braided stream and flood plain ones besides channels, respectively. The change facies assemblage I with facies assemblage ll imply that depositional environments hadbeen migrated from braied sream to flood plain ones.

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A Survey on Diseases and Insect Pests in Sweet Persimmon Export Complexes and Fruit for Export in Korea (단감수출단지 과원과 수출단감 병해충 조사)

  • Jung, Young Hak;You, Eun Ju;Son, Daeyoung;Kwon, Jin Hyeuk;Lee, Dong Woon;Lee, Sang Myeong;Choo, Ho Yul
    • Korean journal of applied entomology
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    • v.53 no.2
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    • pp.157-169
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    • 2014
  • Between 2010 and 2012, diseases and insect pests of sweet persimmon were surveyed at sweet persimmon export complexes and non-export orchards in Suncheon, Jeonnam Province; Jinju, Changwon (Dongeup and Bukmyeon), and Gimhae, Gyeongnam Province; and Ulzu, Ulsan. The following diseases were found in the sweet persimmon orchards: angular leaf spot (Cercospora kaki), anthracnose (Colletotrichum gloeosporioides and Colletotrichum acutatum), circular leaf spot (Mycosphaerella nawae), powdery mildew (Phyllactinia kakicola), and gray mold (Botrytis cinerea). Circular leaf spot was the most frequent and serious disease, and C. gloeosporioides and C. acutatum were found on fruits. Thirty-three insect pest species that belonged to 32 genera of 20 families in 5 orders were found in the sweet persimmon orchards; the two-spotted spider mite, Tetranychus urticae, was also found in the surveyed orchards. Apolygus spinolae, Pseudaulacaspis cockerelli, and Adoxophyes orana were widely found in the surveyed orchards; Spodoptera litura and Homona magnanima were also recorded. Damage by insect pests was low, and the quarantine insect pests peach pyralid moth (Dichocrocis punctiferalis) and persimmon fruit moth (Stathmopoda masinissa) were rarely or not found in the sweet persimmon export complexes. In addition, other quarantine insect pests, such as persimmon false spider mite (Tenuipalpus zhizhilashviliae) and Japanese mealybug (Planococcus kraunhiae), were not detected. These quarantine insect pests were also not found in the sorting places, storage houses, and fruits for export; however, scale insects and two-spotted spider mites were found at a low rate. Although anthracnose (C. acutatum) infested fruit was found in the storage houses, only one in Jinju and Gimhae.

PCA­based Waveform Classification of Rabbit Retinal Ganglion Cell Activity (주성분분석을 이용한 토끼 망막 신경절세포의 활동전위 파형 분류)

  • 진계환;조현숙;이태수;구용숙
    • Progress in Medical Physics
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    • v.14 no.4
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    • pp.211-217
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    • 2003
  • The Principal component analysis (PCA) is a well-known data analysis method that is useful in linear feature extraction and data compression. The PCA is a linear transformation that applies an orthogonal rotation to the original data, so as to maximize the retained variance. PCA is a classical technique for obtaining an optimal overall mapping of linearly dependent patterns of correlation between variables (e.g. neurons). PCA provides, in the mean-squared error sense, an optimal linear mapping of the signals which are spread across a group of variables. These signals are concentrated into the first few components, while the noise, i.e. variance which is uncorrelated across variables, is sequestered in the remaining components. PCA has been used extensively to resolve temporal patterns in neurophysiological recordings. Because the retinal signal is stochastic process, PCA can be used to identify the retinal spikes. With excised rabbit eye, retina was isolated. A piece of retina was attached with the ganglion cell side to the surface of the microelectrode array (MEA). The MEA consisted of glass plate with 60 substrate integrated and insulated golden connection lanes terminating in an 8${\times}$8 array (spacing 200 $\mu$m, electrode diameter 30 $\mu$m) in the center of the plate. The MEA 60 system was used for the recording of retinal ganglion cell activity. The action potentials of each channel were sorted by off­line analysis tool. Spikes were detected with a threshold criterion and sorted according to their principal component composition. The first (PC1) and second principal component values (PC2) were calculated using all the waveforms of the each channel and all n time points in the waveform, where several clusters could be separated clearly in two dimension. We verified that PCA-based waveform detection was effective as an initial approach for spike sorting method.

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Effects of N-Methylacetamide on the Viability, Fertility and Hatchability of Cryopreserved Ogye (Korean Native Black Fowl) Semen (N-Methylacetamide 동결보호제가 오계 동결정액의 생존성, 수정 및 부화율에 미치는 영향)

  • Choi, Jin Seok;Kim, Sung Woo;Shin, Dan-Bi;Ko, Yeoung-Gyu;Do, Yoon-Jung;Kim, Dong-Hun;Kong, Il-Keun;Park, Soo-Bong
    • Korean Journal of Poultry Science
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    • v.39 no.4
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    • pp.291-295
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    • 2012
  • The use of methylacetamide (MA) as a cryoprotective agent for freezing Korean Native Black rooster Ogye semen was examined with artificial insemination. The diluted Ogye semen with HS-1 was subjected for 2 step dilution method of cryopreservation in which the final concentration of MA was adjusted to 7.5%. The sperm viability after thawing was reduced from $95.17{\pm}0.93%$ to $55.93{\pm}1.38%$ which was confirmed by live-death analysis based on Fluorescence-Activated Cell Sorting (FACS). The rates of fertilized eggs with fresh or frozen-thawed semen were reduced from $94.98{\pm}3.93%$ to $66.36{\pm}8.43%$ at day 7 with significant difference. However, the hatching rates of experiments at day 21 did not shown difference between $92.64{\pm}2.33%$ and $90.45{\pm}8.05%$ (P<0.05). With these results, the utilization of MA for freezing of Ogye spermatozoa could affect on viability of frozen-thawed semen but not on the fertility of lain eggs and hatchability of fertilized eggs and also provide possible tools of freezing for poultry genetic resource conservation.

STRUCTURAL MODEL OF CAUSES OF CONDUCT PROBLEM - RELATIONSHIP AMONG CONDUCT PROBLEMS, DEPRESSION, ANXIETY, FAMILY ENVIRONMENT, SELF-CONCEPT, AND TODDLER TEMPERAMENT - (행동문제 원인의 구조적 모델에 관한 연구 - 행동문제, 우울, 불안, 가정환경, 자기개념, 걸음마기 기질의 관계 -)

  • Cho, Soo-Churl;Shin, Min-Sup;Roh, Myoung-Sun
    • Journal of the Korean Academy of Child and Adolescent Psychiatry
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    • v.10 no.1
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    • pp.3-14
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    • 1999
  • Objective:This study was designed to investigate the difference between executive function of Attention Deficit/Hyperactivity Disorder(ADHD) group and that of neurotics, and to investigate the developmental aspects of ADHD group's executive function. Method:Executive function between ADHD(N=87) and Neurotics(N=19) was evaluated through their performance on the Wisconsin Card Sorting Test. The results were analyzed by 2-way ANOVA and t-test. Results:The results revealed group difference between ADHD and neurotics in total correct reponses, total error responses, nonperseverative errors, number of categories completed, conceptual level responses. There was no significant difference between the performance of 8-12 aged group and 13-15 aged group. But 7-8 aged group showed significantly poor performance than 8-12 aged in total responses, total error responses, perseverative responses, perseverative error responses, nonperseverative error responses. Conclusions:In comparison to the neurotics group, the children of ADHD group are suggested to be lacking the ability to correct their responses according to the external feedback and they probably respond randomly without self-control. However, as there is no difference between perseverative errors and perseverative responses, the interpretation of this finding warrants caution. It also suggests that the developmental aspects should be considered in the studies of executive functions because there are differences in the performance of executive functions by ages.

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