• 한국전자파학회논문지
• /
• 제27권5호
• /
• pp.482-485
• /
• 2016

본 논문에서는 쥐 뇌의 감각 피질을 자극하기 위해 변조신호를 이용하여 경두개 자기자극(TMS) 동물실험을 수행하였다. 제안한 TMS 시스템은 인버터, 변압기, 커패시터, 가변 인덕터, 자극 코일로 구성되어 쥐 뇌의 감각피질에 약 1.5 mT의 자기장이 생기도록 시스템을 설계하였다. 자극 신호는 구형파 변조되었으며, 반송 주파수는 85~91 kHz의 범위로 변화시키며, 자극 효과를 관찰하였다. 자극 결과 반송 주파수가 89 kHz 미만일 때에는 자극의 효과를 볼 수 없었고, 반송 주파수가 89 kHz 이상일 때에는 압력 자극에 반응하는 역치 값이 낮아지는 효과를 볼 수 있었다.

• 한국감성과학회:학술대회논문집
• /
• 한국감성과학회 2009년도 추계학술대회
• /
• pp.183-185
• /
• 2009

As imaging technology develops, magnetic resonance imaging (MRI) techniques have contributed to the understanding of brain function by providing anatomical structure of the brain and functional imaging related to information processing. Manganese-enhanced MRI (MEMRI) techniques can provide useful information about functions of the nervous system. However, systematic studies regarding information processing of pain have not been conducted. The purpose of this study was to detect brain activation during painful electrical stimulation using MEMRI with high spatial resolution. Male Sprague-Dawley rats (250-300 g) were divided into 3 groups: normal control, sham stimulation, and electric stimulation. Rats were anesthetized with 2.5% isoflurane for surgery. Polyethylene catheter (PE-10) was placed in the external carotid artery to administrate mannitol and MnCl2. The blood brain barrier (BBB) was broken by 20% D-mannitol under anesthesia mixed with urethane and a-chloralose. The hind limb was electrically stimulated with a 2Hz (10V) frequency while MnCl2 was infused. Brain activation induced by electrical stimulation was detected using a 4.7 T MRI. Remarkable signal enhancement was observed in the primary sensory that corresponds to sensory tactile stimulation at the hind limb region. These results suggest that signal enhancement is related to functional activation following electrical stimulation of the peripheral receptive field.

• 한국의학물리학회지:의학물리
• /
• 제15권3호
• /
• pp.128-133
• /
• 2004

기능적 자기공명영상을 이용하여 진동자의 자극에 따른 감각 피질의 반응에 대하여 연구하였다 주로 40 Hz 이하의 주파수에 반응하는 촉각소체의 혈류반응을 조사하기 위하여 8, 15, 그리고 25 Hz의 주파수를 사용하였다. 사용되고 있는 진동자의 한계를 극복한 공기의 압력을 이용하여 진동할 수 있는 진동자를 특수 제작하였다. 예측한 바와 같이 감각 피질의 촉각소체 부위가 주파수에 따라 반응하는 모습을 볼 수 있었다. 주파수 대 반응 면적을 각 pixel의 개수와 signal percent change로 분석하여 주파수가 증가할수록 signal percent change의 분포는 넓어지고 더 많은 pixel들이 반응한다는 것을 알 수 있었다.

• Annals of Clinical Neurophysiology
• /
• 제1권2호
• /
• pp.106-111
• /
• 1999

Backgroud : The generators of N37 and P37 of posterior tibial nerve somatosensory evoked potential(PTSEP) have not been exactly known. Recently, some reports suggested that P37 and N37 might have different generator. We conducted a study to know the generators of P37 and N37 of PTSEP using gating mechanism. Methods : We evaluated subcortical and cortical somatosensoy evoked potentials(SEPs) in response to posterior tibial nerve stimulation in 3 experimental conditions of foot movement and compared them with PTSEPs in full relaxation of foot. The experimental conditions were: (a) active flexion-extention of stimulated foot, (b) isometric contraction of the stimulated foot, (c) passive flexion-extention of the stimulate foot. We analyzed the latencies and amplitudes of following potentials; P30, N37, P37, and N50. Results : The amplitude of P30 potential did not change during at any paradigms. The amplitudes of P37 and N50 were significantly attenuated in all condition. However, the amplitude of N37 showed no significant change during at any paradigms. Conclusions : These results suggest that the generators of P37 and N37 of PTSEP be different in cortex.

• 한국임상수의학회지
• /
• 제17권2호
• /
• pp.388-394
• /
• 2000

이 실험은 소형견종에 대한 정상 SEPs의 범위를 알아내기 위해 실시되었다. 임상증 상이 정상인 28두를 대상으로 자극점에서 channel 1 까지의 Pl(LPI), channel 1까지의 Nl (LN1), 자극점에서 channel 2가지의 Pl(TP1), channel 2까지의 N1(TNI)의 절대잠복기와 LP1-TN1` 의 파간잠복기를 알아내기 위해서 실시하였다. 이번 실험에서 LPI, LNI, TPI, TNI의 절대잠복기 (absolute latency)의 평균값은 2.69$\pm$0.31 msec, 4.91$\pm$0.49m/sec, 4.64$\pm$0.39 msec, 5.21$\pm$0.42 msec 띠었다. LP1과 TN1 사이의 파간절대잠복기의 핑균값은 2.52$\pm$7.19 msec 이었다. 측정 치들을 속도로 변환하였을 경우 다음과 같았다. 측, LPI, LNI. Tfl, TNI 그리고 LP1-TN1 에서의 속도의 평균값은 각각 93.11$\pm$ 8.58 m/sec, 50.99$\pm$ 5.36m/sec. 80.18$\pm$ sec, 71.31$\pm$4.79m/sec그리고 49.50$\pm$3.58m/sec 이었고. 71.66m/sec, 37.79m/sec, 65.75m/ sec, 59.33 m/sec, 40.55m/sec 의 최저속도를 초과하였을 때 정상범위로 간주하였다. LPI, LNI, TPI,TN1까지의 절대잠복기와 자극전극에 시 측정전극가지의 거리 사이에는 상관관계가 있었다 LP1, LN1, TP1, TN1의 상관계수는 각각 0.621, 0.494. 0.577,0.618 이었다 요추에서 기록된 SEPs갈은 LP1의 상관계수가 LN1 보다 높았으며 흉추에서 기록된 SEPs값은 TN1의 상관계수가 TP1보다 높았다. LP1과 TN1의 파간잠복기와 channel 1과 2의 거리차이와의 상관계수는 0.571이다. 따라서 LPI, LNI. TPI, TNI그리고 LPI-TNI 들의 최저속도를 이용 하여 척수 손상 여부를 판단할 수 있다고 생각된다.

• 대한물리의학회지
• /
• 제15권1호
• /
• pp.25-32
• /
• 2020

PURPOSE: This study examined the efficacy of an interferential current (IFC) treatment on the improvement of pain, disability, and quality of life in stroke patients with lumbago. METHODS: A double-blind, randomized clinical trial was conducted on 40 stroke patients with lumbago. The patients were allocated randomly into two groups: the IFC treatment group (n= 20) and the placebo treatment group (n= 20). The IFC group received 30 minutes of IFC treatment on the lumbar region, while the placebo group received IFC treatment but without real electrical stimulation. The intervention was administered five days a week for four weeks. The primary outcomes of pain intensity were measured using a visual analogue scale. The secondary measurements included the Barthel Index, Oswestry Disability Index (ODI), and health-related quality of life (HRQoL). RESULTS: The measurements were conducted before and after the two-week intervention period. Compared to the placebo treatment group, the IFC treatment group showed significantly greater improvement in the pain intensity (p<.05), ODI (p<.05), and SF-36 (p<.05) at the end of the intervention. No significant differences in the Barthel Index were found between the two groups. CONCLUSION: These findings show that an IFC treatment can improve pain, functional ability, and quality of life, highlighting the benefits of somatosensory stimulation from IFC in stroke patients with lumbago.

• 대한의생명과학회지
• /
• 제11권3호
• /
• pp.365-373
• /
• 2005

The aim of this study was to investigate the alterations in meningeal blood flow by stimulation of trigeminovascular system. An open cranial window was prepared on the right parietal bone of male Sprague-Dawley rats. Trigeminovascular system was stimulated by electrical stimulation of trigeminal ganglion (ETS), somatosensory (whisker) stimulation, or topical applications of capsaicin and neuropeptides including substance P and calcitonin gene-related peptide (CGRP). Neonatal capsaicin pretreatment was performed with subcutaneous administration of capsaicin (50 mg/kg) within the first 24 hours after birth. Changes in regional blood flow of dural artery (rDBF) and pial artery (rPBF) were continuously measured through the cranial window by laser-Doppler flowmetry. Both ETS and capsaicin caused a chain of alterations in rPBF and rDBF responses, i.e., an immediate transient decrease followed by rapid and marked increase in rPBF, which were significantly attenuated not only by pretreatments with L-733,060, a $NK_1$ receptor blocker, $CGRP_{8-37}$, a $CGRP_1$ receptor blocker, and 7-nitroindazole monosodium salt (7-NINA), a neuronal nitric oxide synthase inhibitor but also by neonatal capsaicin treatment. Exogenous neuropeptides including substance P and CGRP increased the meningeal blood flow, which was significantly attenuated not only by pretreatment with L-733,060 and $CGRP_{8-37}$, respectively, but also by pretreatment with 7-NINA. The rPBF response to whisker stimulation was significantly attenuated not only by trigeminovascular system injuries including nasociliary nerve denervation and neonatal capsaicin treatment but also by pretreatments with L-733,060, $CGRP_{8-37}$ and 7-NINA. These results suggest that the stimulation of trigeminovascular system causes prominent alterations in meningeal blood flow, and that neuropeptides as well as nitric oxide in the trigeminovascular system are importantly implicated in the regulation of meningeal blood flow.

• 한국전문물리치료학회지
• /
• 제27권3호
• /
• pp.191-198
• /
• 2020

Background: Stroke patients experience multiple dysfunctions that include motor and sensory impairments. Therefore, new intervention methods require a gradational approach depending on functional levels of a stroke patient's activity and should include cognition treatment to allow for a patient's active participation in rehabilitation. Objects: This study investigates the effect of integrated revision of electrical sensory stimulation, which stimulates somatosensory and action observation training, which is synchronized cognition intervention method on stroke patients' functions. Methods: Twenty-one stroke patients were randomized into two groups. The two groups underwent twenty minutes of intervention five times a week for three weeks. This study used an electromyogram to evaluate symmetric muscle activation of lower extremities and muscle onset time when performing sit to stand before and after intervention. A weight-bearing ratio was used to evaluate the weight-bearing of the affected side in a sit to standing. To evaluate sit to stand performance ability, this study performed five timed sit to stand tests. Results: The two groups both showed statistically significant improvement in muscle onset time of lower extremity, static balance ability in a standing position, and sit to stand performance after the intervention (p < 0.05). In addition, the action observation and synchronized electrical sensory stimulation group showed significant improvement in symmetric muscle activation of lower extremities and weight-bearing ratio of the affected side (p < 0.05). Conclusion: action observation and synchronized electrical sensory stimulation (AOT with ESS) can have positive effects on a stroke patient's sit to stand performance, and the intervention method that provides integrated AOT with ESS can be used as new nervous system intervention program.

• 한국임상수의학회지
• /
• 제19권3호
• /
• pp.277-282
• /
• 2002

본 실험은 각각의 마취방법이 체성감각유발전위 (SEPs) 파형에 미치는 영향을 알아보고 SEP의 측정에 적절한 마취방법을 찾고자 시행하였다. 임상적으로 건강하고 크기와 나이가 비슷한 다섯 마리의 잡종견을 대상으로 SEPs를 측정하고 각각의 측정값을 분석하였다. SEPs측정을 위해 후 경골신경을 자극하였고 요추 5-6번 사이에서 channel 1의 LP1과 LN1, 흉추 11-12사이에서 channel 2의 TP1, TN1을 기록하였다. 실험에 사용한 마취방법 중. Acepromazine + Thiopental Na + Isoflurane, Acepromazine + Propofol + Isoflurane, Diazepam + Xylazine, Xylazine + Ketamine, Acepromazine + Propofol infusion, 및 Propofol infusion등의 방법만이 SEPs측정이 가능하였고, 파형은 명확하였으며, 측정에 요구되어지는 일정 시간인 25분 이상 동안 마취 유지가 가능하였다. 또한 각 마취군에서의 SEPs 파형을 Acepromazine + Thiopental Na + Isoflurane군과 비교해 보았을 때 latency의 경우, Acepromazine + Propofol + Isoflurane군의 ST(stimulating point)-LN1, SP-TP1, Diazepam + Xylazine 군의 Chl-Ch2, Xylazine + Ketamine군의 Chl-Ch2, Acepromazine + Propofol infusion군의 ST-LP1와 Chl-Ch2에서 부분적으로 유의적인 차가 있었다. Conduction velocity의 경우, Acepromazine + Propofol + Isoflurane군의 ST-LN1, Diazepam + Xylazine군의 Chl-Ch2, Xylazine + Ketamine군의 Chl-Ch2, Acepromazine + Propofol infusion군의 ST-LP1, 그리고 Propofol infusion군의 ST-LN1의 측정값에서 유의적인 차가 있었지만 전반적으로는 전체적인 파형의 유의적인 변화는 없었다. 이상의 결과를 토대로 SEPs 측정시 흡입마취로는 Acepromazine + Thiopental + Isoflurane과 Acepromazine + Propofol + Isoflurane, 주사마취로는 Diazepam + Xylazine과 Xylazine + Ketamine, 점적마취로는 Acepromazine + Propofol infusion과 Propofol infusion 방법이 사용 가능한 것으로 확인되었다.

• Journal of Korean Neurosurgical Society
• /
• 제29권8호
• /
• pp.985-994
• /
• 2000

Objective : There are some advantages of trigeminal evoked potential(TEP) recording compared to other somatosensory evoked potential(SSEP) recordings. The trigeminal sensory pathway has a pure sensory nerve branch, a broader receptive field in cerebral cortex, and a shorter pathway. Despite these advantages, there is little agreement as to what constitutes a normal response and what wave forms truly characterize the intraoperative TEP. This study presents the normative data of TEP recorded on the epidural surface of the rat with a platinum ball electrode. Materials & Methods : Under general anesthesia with urethane, the adult Sprague-Dawley male rats(300-350g) were given electrical stimulation with two stainless steel electrodes which were inserted into the subcutaneous layer of the area around whiskers. A reference electrode was positioned in the temporalis muscle ipsilateral to the recording site. Results : TEPs were recorded in the Par I area of somatosensory cortex and recorded most apparently on the point of 2mm posterior from the bregma and 6mm lateral from the midline. The typical wave form consisted of 5 peaks (N1-P1-N2-P2-N3 according to emerging order, upward negativity). Each latency to corresponding peaks was not influenced by the different intensities of stimulation, especially from 1 to 5mA. Average latencies of 5 peaks were in the following order ; 7.7, 11.1, 15, 22.3, 29.4ms. There was also no significant difference between latencies before and after administration of muscle relaxant(pancuronium). For the electrophysiological localization of recorded waves, the action potential of a single unit was recorded with glass microelectrode(filled with 2M NaCl, $3-5M{\Omega}$) in the thalamus of rat. A sharp wave was recorded in the VPM nucleus, in which the latency was shorter than that of N1. This suggests that all 5 peaks were generated by neural activities in the suprathalamic pathway. Conclusion : In terms of recording near-field potentials, our data also suggests that TEP in the rat may be superior to other SSEPs. In overall, these results may afford normative data for the studies of supratentorial lesions such as hydrocephalus or cerebral ischemia which can have an influence on near-field potentials.