• 한국식품과학회지
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• 제25권6호
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• pp.698-702
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• 1993

온도 증가에 따라 용출되는 soluble carbohydrate는 온도가 증가할수록 더 큰 분자들이 많이 용출되었고 동부나 녹두는 아주 유사한 용출 양상을 보였다. 그리고 팥은 동부나 녹두보다는 분자량이 작지만 강낭콩보다는 큰 분자들이 용출되었고 $95^{\circ}C$에서의 용출양상이 동부나 녹두의 $85^{\circ}C$에서의 용출양상과 비슷해지는 것을 알 수 있었다. 그러나 강낭콩은 온도가 증가되어도 아주 작은 분자들이 계속 많이 용출되어 강낭콩 아밀로오스가 작은 분자들로 이루어져 있다는 것을 시사했다. 텍스쳐실험결과 전분겔의 특성은 녹두가 가장 견고성이 크고 동부는 응집성이 가장 컸다. 팥이나 강낭콩은 견고성과 응집성 모두 동부나 녹두에 비해 낮았다. 15분간 가열하여 제조한 전분겔의 경우 가열온도가 증가할수록 견고성과 응집성이 증가하였다. 1시간 가열하여 제조한 전분겔의 경우 동부와 녹두는 $85^{\circ}C$에서보다 $95^{\circ}C$에서 견고성이 감소하였으나 팥이나 강낭콩은 가열온도 증가에 따라 견고성이 증가하였고 시료 전분겔 모두 응집성은 증가 하는 양상을 보였다.

• Journal of Ecology and Environment
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• 제34권3호
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• pp.251-257
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• 2011

The purpose of this study was to investigate the acclimation responses of Tamarix chinensis to cold stress. We evaluated the acclimation responses by measuring biomass, daily elongation rate, chlorophyll content, and total soluble carbohydrate content. The plant samples comprised leaves from seedlings of 2 different ages (8 and 12 weeks); the leaves were collected 0, 2, and 4 weeks after cold treatment. We found that the cold-treated samples showed reduced daily elongation rates and chlorophyll content. Further, these samples showed more than 8-fold increase in the total soluble carbohydrate content. However, the seedling ages did not have a significant influence on the growth of cold-treated seedlings. On the basis of these findings, we can conclude that T. chinensis seedlings aged less than 1 year old show acclimation to cold stress by accumulating soluble carbohydrates. This study may help us understand how T. chinensis seedlings acclimatize to their first cold season.

• 한국약용작물학회지
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• 제23권4호
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• pp.292-297
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• 2015

This study was carried out to have the basic and applied informations relating to develop the cultivation methods and to increase the productivity and quality of ginseng. 1 to 6 year old ginsengs of Jakyung cultivar were cultivated and the content and synthetic amount of carbohydrates were investigated with different plant tissues, growth stages, and years old. The concentration of total carbohydrates at six year old ginseng including water soluble and water insoluble carbohydrates was about 18.9%, 42.9%, and 43,6% in leaves, tap roots, and lateral roots, respectively. Water soluble carbohydrate of tap and lateral roots was slightly decreased from August until September, and then increased on November, whereas its water insoluble carbohydrate was increased from August to September and then decreased on November. Comparing with the content of carbohydrates of 1 to 6 year old ginsengs, it was continuously increased from one year old ginseng until five year old ginseng, however it was not increased much in six year old ginseng. The highest content of carbohydrates was at five year-old in all tissues of ginseng. Water soluble and water insoluble carbohydrates were significantly shown different in leaves, stems, tap roots, and lateral root at different growth stages and with different years old. The content of water soluble carbohydrate in the leaves was remarkedly higher compared to that of water insoluble carbohydrate, while in the root the content of water insoluble carbohydrate was clearly higher compared to the water soluble carbohydrate. Comparing with the synthetic amount of carbohydrates, water soluble carbohydrates was higher in the shoot than that in the root, whereas water-insoluble carbohydrates higher in the root than that in the shoot. Carbohydrates which would be utilized in ginseng tissues for short and long-term periods as major energy were appeared differently in between shoot and root, with different growth stages, and years old.

• Asian-Australasian Journal of Animal Sciences
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• 제22권3호
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• pp.356-364
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• 2009

The current ruminant feeding models require parameterization of the digestion kinetics of carbohydrate fractions in feed ingredients to estimate the supply of nutrients from a ration. Using an automated gas production technique, statistically welldefined digestion rate of carbohydrate, including soluble carbohydrate, can be estimated in a relatively easy way. In this study, the gas production during in vitro fermentation was measured and recorded by an automated gas production system to investigate degradation kinetics of carbohydrate fractions of a wide range of ruminant feeds: corn silage, rice straw, corn, soybean hull, soybean meal, and cell mass from lysine production (CMLP). The gas production from un-fractionated, ethanol insoluble residue and neutral detergent insoluble residue of the feed samples were obtained. The gas profiles of carbohydrate fractions on the basis of the carbohydrate scheme of the Cornell Net Carbohydrate and Protein System (A, B1, B2, B3 and C) were generated using a subtraction approach. After the gas profiles were plotted with time, a curve was fitted with a single-pool exponential equation with a discrete lag to obtain kinetic parameters that can be used as inputs for modern nutritional models. The fractional degradation rate constants (Kd) of corn silage were 11.6, 25.7, 14.8 and 0.8%/h for un-fractioned, A, B1 and B2 fractions, respectively. The values were statistically well estimated, assessed by high t-value (>12.9). The Kd of carbohydrate fractions in rice straw were 4.8, 21.1, 5.7 and 0.5%/h for un-fractioned, A, B1 and B2 fractions, respectively. Although the Kd of B2 fraction was poorly defined with a t-value of 4.4, the Kd of the other fractions showed tvalues higher than 21.9. The un-fractioned corn showed the highest Kd (18.2%/h) among the feeds tested, and the Kd of A plus B1 fraction was 18.7%/h. Soybean hull had a Kd of 6.0, 29.0, 3.8 and 13.8%/h for un-fractioned, A, B1 and B2, respectively. The large Kd of fraction B2 indicated that NDF in soybean hull was easily degradable. The t-values were higher than 20 except for the B1 fraction (5.7). The estimated Kd of soybean meal was 9.6, 24.3, 5.0%/h for un-fractioned, A and B1 fractions, respectively. A small amount of gas (5.6 ml at 48 ho of incubation) was produced from fermentation of CMLP which contained little carbohydrate. In summary, the automated gas production system was satisfactory for the estimation of well defined (t-value >12) kinetic parameters and Kd of soluble carbohydrate fractions of various feedstuffs that supply mainly carbohydrate. The subtraction approach, however, should be applied with caution for some concentrates, especially those which contain a high level of crude protein since nitrogen-containing compounds can interfere with gas production.

• Pediatric Gastroenterology, Hepatology & Nutrition
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• 제23권2호
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• pp.163-173
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• 2020

Purpose: This study aimed to investigate the clinical and metabolic determinants of circulating soluble leptin receptor (CSLR) and free leptin index (FLI) in pre-pubertal obese male children. Methods: We conducted a preliminary cross-sectional study at three tertiary hospitals and one public primary school. Eighty obese male children without growth and developmental abnormalities aged 5-9 years were recruited. In these children, obesity was solely caused by excessive food intake, and not by acute illness, medications, endocrine abnormalities, or any syndrome. Body mass index (BMI), body fat mass, carbohydrate intake, fat intake, high density lipoprotein cholesterol level, low density lipoprotein cholesterol level, triglyceride level, and Homeostatic Model Assessment for Insulin Resistance are the potential determinants for leptin regulation, which is represented by CSLR level and FLI. Results: Carbohydrate was the main source of energy. BMI and body fat mass had negative weak correlation with CSLR and positive weak correlation with FLI. Furthermore, carbohydrate intake was found to be independently associated with CSLR based on the results of the multiple linear regression analysis. Following an increase in carbohydrate intake, CSLR level decreased progressively without any negative peak. Conclusion: Leptin regulation in prepubertal obese male children is associated with body composition and dietary intake. Carbohydrate intake is useful for predicting CSLR. Lipid profiles and insulin resistance are not related to both CSLR and FLI. Treatment and prevention of leptin resistance in obese children should focus on reducing BMI, fat mass, and carbohydrate intake.

• 미생물학회지
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• 제6권2호
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• pp.41-53
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• 1968

Studies on the carbohydrate metabolism of yeast as influenced by gamma-irradiation from cobalt-60 have been carried, then the mechanisms of radiation effect on respiration and fermentation were discussed under considerations of permeable changes of irradiated cell membrane. The cells of baker's yeast (Saccharomyces cerevisiae) which had been gamma-irradiated of 240 k.r. doses for an hour, then were put into aerobic oxidation and anaerobic fermentation without substrate. Total and fractionated carbohydrates of irradiated yeast cells were determined by calorimetric method with anthrone and orcinol reagents, the amounts of total carbohydrate, trehalose, RNA-ribose, PCA-soluble glycogen, alkali-soluble glycogen, acetic acid-soluble glycogen, mannan and glucan were determined according to the course of aerobic oxidation and anaerobic fermentation. It is found that the carbohydrates of irradiated cells leak out and amount of the losses teaches eleven times more than that of control, the volume of losses are seems to be replaced by water, it can be suggested the damage of gamma-irradiation occurs in the site of passive transport of cell membrane. The endogeneous aerobic respiration of irradiated cells are increased much more than control, the synthesis of reserve glycogen, glucan and RNA-ribose promoted much more than control. The anaerobic fermentation of irradiated cells are also increased than that of control, but the breakdown of carbohydrate is less than endogeneous respiration of irradiated cells. The synthetic rate is also less than that of aerobic oxidation. In irradiated yeast cells, trehalose is revealed to be primary substrate for endogeneous carbohydrate metabolism, so it is proved that the enzymic patterns are not changed but the activities of enzymes relating endogeneous respiration and autofermentation is activated. It is to be considerable to distiguish endogeneous respiration and autofermentation from exogeneous respiration and fermentation on irradiation, for membrane permeability changes and loses out carbohydrate by ionizing radiation.

• 한국토양비료학회지
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• 제47권6호
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• pp.563-570
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• 2014

The shortage or surplus of minerals directly affects overall physiological metabolism of plants; especially, it strongly influences carbohydrate metabolism as a primary response. We have studied mineral uptake, synthesis and partitioning of soluble carbohydrates, and the relationship between them in N, P or K-excessive tomato plants, and examined the interaction between soluble carbohydrates and mineral elements. Four-weeks-old tomato plants were grown in a hydroponic growth container adjusted with excessive N ($20.0mmol\;L^{-1}$ $Ca(NO_3)2{\cdot}4H_2O$ and $20.0mmol\;L^{-1}$ $KNO_3$), P ($2.0mmol\;L^{-1}$ $KH_2PO_4$), and K ($20.0mmol\;L^{-1}$ $KNO_3$), respectively, for 30 days. Shoot growth rates were significantly influenced by excessive N or K, but not by excessive P. The concentrations of water soluble N (nitrate and ammonium), P and K were clearly different with each tissue of tomato plants as well as the mineral conditions. The NPK accumulation in all treatments was as follows; fully expanded leaves (48%) > stem (19%) = roots (16%) = petioles (15%) > emerging leaves (1). K-excessive condition extremely contributed to a remarkable increase in the ratio, which ranged from 2.79 to 10.34, and particularly potassium was dominantly accumulated in petioles, stem and roots. Fresh weight-based soluble sugar concentration was the greatest in NPK-sufficient condition ($154.8mg\;g^{-1}$) and followed by K-excessive (141.6), N-excessive (129.2) and P-excessive (127.7); whereas starch was the highest in K-excessive ($167.0mg\;g^{-1}$) and followed by P-excessive (146.1), NPK-sufficient (138.2) and N-excessive (109.7). Soluble sugar showed positive correlation with dry weight-based total N content (p<0.01) whereas was negatively correlated with soluble P (p<0.01) and dry weight-based total P (p<0.01). On the other hand, starch production was negatively influenced by total N (p<0.001), but, it showed positive relation with total K concentration (p<0.05). This study shows that uptake pattern of NPK and production and partitioning of soluble carbohydrate were substantially different from each mineral, and the relationship between water soluble- and dry weight-based-mineral was positive.

• 동아시아식생활학회지
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• 제16권6호
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• pp.740-746
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• 2006

The proximates, vitamin C, minerals, and fatty acids of five potato cultivars were evaluated by AOAC methods, the hydrazine method, ICP-AES, and gas chromatography, respectively. The proximate analyses; vitamin C, reducing sugar, and soluble solid contents; and mineral and fatty acid compositions were significantly different among the five cultivars. The Superior cultivar contained a higher carbohydrate content and higher Ca/P ratio and lower levels of crude protein and Na. The Atlantic cultivar contained significantly higher amount of energy, carbohydrate, reducing sugar, vitamin C, SEA, and MUFA, and significantly lower amount of minerals and PUFA. The Shepody cultivar contained significantly higher amount of carbohydrate and MUFA, and significantly lower amount of soluble solid, vitamin C, and SFA. In addition, the P, Fe, Mg, Cu, and Al levels were significantly higher in Shepody, and Zn content was significantly lower. The Jopung cultivar contained significantly higher levels of moisture and Na, and significantly lower levels of soluble content, reducing sugar, carbohydrate, crude protein, and fat. Finally, the Namsuh cultivar contained significantly higher amount of soluble solid, crude protein, K, Mg, and Al, and significantly lower amount of reducing sugar.

• 한국토양비료학회지
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• 제46권5호
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• pp.351-358
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• 2013

A suitable supply of mineral elements into shoot via a root system from growth media makes plants favorable growth and yield. The shortage or surplus of minerals directly affects overall physiological reactions to plants and, especially, strongly influences carbohydrate metabolism as a primary response. We have studied mineral uptake and synthesis and translocation of soluble carbohydrates in N, P or K-deficient tomato plants, and examined the interaction between soluble carbohydrates and mineral elements. Four-weeks-old tomato plants were grown in a hydroponic growth container adjusted with suboptimal N ($0.5mmol\;L^{-1}\;Ca(NO_3)2{\cdot}4H_2O$ and $0.5mmol\;L^{-1}\;KNO_3$), P ($0.05mmol\;L^{-1}\;KH_2PO_4$), and K ($0.5mmol\;L^{-1}\;KNO_3$) for 30 days. The deficiency of specific mineral element led to a significant decrease in its concentration and affected the concentration of other elements with increasing treatment period. The appearance of the reduction, however, differed slightly between elements. The ratios of N uptake of each treatment to that in NPK sufficient tomato shoots were 4 (N deficient), 50 (P deficient), and 50% (K deficient). The P uptake ratios were 21 (N deficient), 19 (P deficient), and 28% (K deficient) and K uptake ratios were 11 (N deficient), 46 (P deficient), and 7% (K deficient). The deficiency of mineral elements also influenced on carbohydrate metabolism; soluble sugar and starch was substantially enhanced, especially in N or K deficiency. In conclusion, mineral deficiency leads to an adverse carbohydrate metabolism such as immoderate accumulation and restricted translocation as well as reduced mineral uptake and thus results in the reduced plant growth.

• Journal of Ginseng Research
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• 제17권1호
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• pp.13-21
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• 1993

We attempted in this study to understand the hypoglycemic action of the fat soluble fraction of red ginseng roots in streptozotocin injected diabetic rats, through its actions on several enzymes relating to carbohydrate metabolism of the 1eve1 to compare with those of ginsenosides in streptozotocin injected diabetic rats. It was realized that the increased level of glucose, ketone bodies, lactate, nonesterified fatty acids and triacylglycerol in blood was significantly decreased and the decreased liver glycogen content of streptozotocin injected rats were appreciably moderated by intraperitoneal injection of the fat soluble fraction of red ginseng roots as shown in the saponin injected diabetic rats. The deceased activities of liver enzymes relating to carbohydrate metabolism such as phosphofructokinase, glucokinase, glucose-6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase and acetyl CoA carboxylase of streptozotocin induced diabetic rats were also sufficiently modified by the intraperitoneal injection of the above fat soluble fraction as shown in the ginsenoside injected streptozotocin induced rats.