• Title/Summary/Keyword: Solidification

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Establishment of a novel plant regeneration system from suspension-derived callus in the halophytic Leymus chinensis (Trin.)

  • Sun, Yan-Lin;Hong, Soon-Kwan
    • Journal of Plant Biotechnology
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    • v.37 no.2
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    • pp.228-235
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    • 2010
  • The establishment of cell suspension culture and plant regeneration of the halophytic Leymus chinensis (Trin.) are described in this study for the first time. Callus induction solid medium containing Murashige and Shoog (MS) basic salt, $2.0\;mg\;l^{-1}$ 2,4-dichlorophenoxyacetic acid (2,4-D), and $5.0\;mg\;l^{-1}$ L-glutamic acid with $30.0\;g\;l^{-1}$ sucrose and $4.0\;g\;l^{-1}$ gelrite for solidification induced the highest rate of cell division in Type 1 callus among calli of various types. Liquid medium with the same hormone distribution was therefore, used for cell suspension culture from Type 1 callus. Over a 30 d suspension culture at 100 rpm, great amounts of biomass were accumulated, with 71.07% average daily increment and 22.32-fold total fresh weight increment. Comparison of before and after suspension culture, the distribution of different size callus pieces and the maintenance of callus type were basically unaltered, but a slight increase in relative water contents was observed. To induce the potential of plant regeneration, the directly transferring on plant regeneration solid medium containing MS basic salt, $0.2\;mg\;l^{-1}$ $\alpha$-naphthalene acetic acid (NAA), $2.0\;mg\;l^{-1}$ kinetin (Kn), and $2.0\;g\;l^{-1}$ casamino acid and indirectly transferring were simultaneously performed. Even now growth rates of suspension-derived callus on solid medium were approximately half of those of Type 1 callus, but faster somatic embryogenesis was observed. Rooting of all regenerated shoots was successfully performed on half-strength MS medium. All plants appeared phenotypically normal.

Effect of Cr/Ni equivalent ratio on ductility-dip cracking in AISI 316L austenitic stainless steel weld metals ($Cr_{eq}/Ni_{eq}$ 당량비에 따른 AISI 316L 스테인리스강의 연성저하균열 특성에 대한 연구)

  • Jang, A.Y.;Lee, D.J.;Kim, Y.H.;Choi, C.H.;Lee, S.H.;Byun, J.C.;Jung, G.H.;Lee, H.W.
    • Proceedings of the KWS Conference
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    • 2009.11a
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    • pp.56-56
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    • 2009
  • AISI 316L 스테인리스강에 새롭게 디자인한 서로 다른 3가지 응고모드를 가진 와이어로 FCAW(Flux Cored Arc Welding)을 하였다. 각각의 3가지 와이어는 Pseudobinary phase diagram에 따라 AF, FA, F모드를 가졌다. 미세조직은 $Cr_{eq}/Ni_{eq}$이 증가할수록 델타 페라이트 함량이 증가하였으며, 초정 상의 경우 초정 오스테나이트에서 초정 페라이트로 변태하였고, 연성저하균열의 민감도가 감소하였다. 연성저하균열은 이동결정립계의 형상에 따라 좌우되며, 미량의 페라이트를 함유한 오스테나이트에서는 페라이트가 핀(Pin) 역할을 제대로 하지 못하여 직선형태의 이동 결정립계 따라 입계 미끄러짐의 메커니즘을 통해 전파되었으며, 곡선형태의 이동 결정립계에서는 델타 페라이트가 핀 역할을 하여 역할을 하여 구속 상태에서 응력집중을 막고 응력을 분산시켜 균열이 전파되는 것을 방해하여 균열이 발생되지 않았다.

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Influence of Electrolytic KF on the Uniform Thickness of Oxide Layers Formed on AZ91 Mg Alloy by Plasma Electrolytic Oxidation

  • Song, Duck-Hyun;Lim, Dae-Young;Fedorov, Vladimir;Song, Jeong-Hwan
    • Korean Journal of Materials Research
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    • v.27 no.9
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    • pp.495-500
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    • 2017
  • Oxide layers were formed by an environmentally friendly plasma electrolytic oxidation (PEO) process on AZ91 Mg alloy. PEO treatment also resulted in strong adhesion between the oxide layer and the substrate. The influence of the KF electrolytic solution and the structure, composition, microstructure, and micro-hardness properties of the oxide layer were investigated. It was found that the addition of KF instead of KOH to the $Na_2SiO_3$ electrolytic solution increased the electrical conductivity. The oxide layers were mainly composed of MgO and $Mg_2SiO_4$ phases. The oxide layers exhibited solidification particles and pancake-shaped oxide melting. The pore size and surface roughness of the oxide layer decreased considerably with an increase in the concentration of KF, while densification of the oxide layers increased. It is shown that the addition of KF to the basis electrolyte resulted in fabricating of an oxide layer with higher surface hardness and smoother surface roughness on Mg alloys by the PEO process. The uniform thickness of the oxide layer formed on the Mg alloy substrates was largely determined by the electrolytic solution with KF, which suggests that the composition of the electrolytic solution is one of the key factors controlling the uniform thickness of the oxide layer.

Effect of welding condition on microstructures of weld metal and mechanical properties in Plasma-MIG hybrid welding for Al 5083 alloy (알루미늄 5083 합금의 플라즈마 미그 하이브리드 용접시 용접부 미세조직과 기계적 성질 변화에 미치는 용접조건의 영향)

  • Park, Sang-Hyeon;Lee, Hee-Keun;Kim, Jin-Young;Chung, Ha-Taek;Park, Young-Whan;Kang, Chung-Yun
    • Journal of Welding and Joining
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    • v.33 no.1
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    • pp.61-71
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    • 2015
  • The effect of welding condition on microstructure and mechanical property of Plasma-MIG Hybrid Weld between Al 5083 plates(thickness : 10mm) was investigated. 1 pass weld without any defects such as puckering, undercut, and lack of fusion was obtained by 150~200A of plasma current and 5~7mm of welding speed. Gas porosities and shrinkage porosities were existed in the weld near fusion line. As welding speed and plasma current were decreasing, the area fraction of porosity was increasing. The hardness of the weld is increasing as welding speed. On the basis of microstructural analysis, Mg segregated region near dendrite boundaries tends to increase with the welding speed. In the result of hardness test, Distribution of hardness in fusion zone showed little change with the plasma current. However, when the welding speed increased, hardness in weld metal markdly increased. It could be considered that effect of heat input to growth of the dendritic solidification structures. Based on tensile test, tensile properties of weld metal was predominated by area fraction of porosities. Consequently, tensile properties can be controlled by formation site and area fraction of porosity.

Assessment of asbestos exposure level of workers handling waste containing asbestos (석면함유폐기물 취급근로자의 석면노출수준 평가)

  • Jeong, Jee Yeon;Kim, Eun Young
    • Journal of Korean Society of Occupational and Environmental Hygiene
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    • v.28 no.2
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    • pp.135-143
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    • 2018
  • Objectives: There have been many studies on exposure assessment of workers at companies using asbestos as a raw material and at sites of the removal of materials containing asbestos. However, no research has been carried out on the asbestos exposure of workers in industries involving asbestos-containing waste, such as workers at collection and transportation service companies, mid-treatment companies(solidification of asbestos-containing waste), and landfill sites. The objective of this study was to assess the asbestos exposure concentrations of workers in industries handling waste containing asbestos. Methods: For this study, we carried out field investigations at 15 companies: seven collection and transportation service companies, three mid-treatment companies, and five final treatment companies(landfill sites). We took both personal and area samples. Results: The range of asbestos exposure levels of workers handing asbestos-containing wastes at collection, mid-treatment, and landfill companies were 0.000 fibers/cc-0.009 fibers/cc, 0.000 fibers/cc-0.038 fibers/cc, and 0.000 fibers/cc-0.024 fibers/cc, respectively. Conclusions: The asbestos exposure levels of workers at mid-treatment companies were higher than those at collection and transportation companies and at final treatment companies. In the case of collection and transportation workers, the possibility of exposure to levels exceeding those found in the present study is not particularly high considering the characteristics of the work. However, in the case of intermediate or final disposal workers, it is considered that there is a possibility of exposure to levels above those found in this study.

An Investigation of Dispersion Behavior of Y2O3 Ceramic Particles in Hypo, Eutectic and Hyper Binary Al-Cu Cast Alloys (아공정, 공정, 과공정 조성의 Al-Cu 주조합금에서의 Y2O3 분말의 분산 거동에 대한 연구)

  • Park, J.J.;Kim, G.H.;Hong, S.M.;Lee, S.H.;Lee, M.K.;Rhee, C.K.
    • Journal of Powder Materials
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    • v.14 no.2 s.61
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    • pp.123-126
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    • 2007
  • In this work, the dispersion behavior of $Y_2O_3$ particles in binary aluminum (Al)-copper (Cu) cast alloy was investigated with respect to Cu contents of 20 (hypoeutertic), 33 (eutectic) and 40 (hypereutectic) wt.%. In cases of hypo and hypereutectic compositions, SEM images revealed that the primary Al and ${\theta}$ phases were grown up at the beginning, respectively, and thereafter the eutectic phase was solidified. In addition, it was found that some of $Y_2O_3$ particles can be dispersed into the primary Al phase, but none of them are is observed inside the primary 6 phase. This different dispersion behavior of $Y_2O_3$ particles is probably due to the difference in the val- ues of specific gravity between $Y_2O_3$ particles and primary phases. At eutectic composition, $Y_2O_3$ particles were well dispersed in the matrix since there is few primary phases acting as an impediment site for particle dispersion during solidification. Based on the experimental results, it is concluded that $Y_2O_3$ particles are mostly dispersed into the eutectic phase in binary Al-Cu alloy system.

Investigation of Spark Plasma Sintering Temperature on Microstructure and Thermoelectric Properties of p-type Bi-Sb-Te alloys

  • Han, Jin-Koo;Shin, Dong-won;Madavali, Babu;Hong, Soon-Jik
    • Journal of Powder Materials
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    • v.24 no.2
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    • pp.115-121
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    • 2017
  • In this work, p-type Bi-Sb-Te alloys powders are prepared using gas atomization, a mass production powder preparation method involving rapid solidification. To study the effect of the sintering temperature on the microstructure and thermoelectric properties, gas-atomized powders are consolidated at different temperatures (623, 703, and 743 K) using spark plasma sintering. The crystal structures of the gas-atomized powders and sintered bulks are identified using an X-ray diffraction technique. Texture analysis by electron backscatter diffraction reveals that the grains are randomly oriented in the entire matrix, and no preferred orientation in any unique direction is observed. The hardness values decrease with increasing sintering temperature owing to a decrease in grain size. The conductivity increases gradually with increasing sintering temperature, whereas the Seebeck coefficient decreases owing to increases in the carrier mobility with grain size. The lowest thermal conductivity is obtained for the bulk sintered at a low temperature (603 K), mainly because of its fine-grained microstructure. A peak ZT of 1.06 is achieved for the sample sintered at 703 K owing to its moderate electrical conductivity and sustainable thermal conductivity.

Pharmacokinetic Behavior and Biodistribution of Paclitaxel-Loaded Lipid Nanosuspension

  • Choi, Sung-Up;Park, Jung-Min;Choi, Woo-Sik;Lee, Jae-Hwi;Choi, Young-Wook
    • Journal of Pharmaceutical Investigation
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    • v.39 no.5
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    • pp.359-366
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    • 2009
  • In this study, paclitaxel-loaded lipid nanosuspension (PxLN) was prepared and the in vivo profiles after intravenous administration in rats were investigated. We compared the manufacturing processes depending on the temperature: PxLN-H for a hot homogenization process and PxLN-C for solidification of lipid-drug mixtures by liquid nitrogen. Both formulations showed submicron size distribution and the similar drug loading efficiency of about 70%. In vitro release of PxLNs and Taxol$^{(R)}$ performed by a dialysis diffusion method showed similar pattern for PxLN-H and Taxol$^{(R)}$, but the reduced release profile for PxLN-C. PxLN or Taxol$^{(R)}$ was intravenously administered to the rats at a dose of 5 mg/kg as paclitaxel. The drug in blood samples were assayed by the HPLC/MS/MS method. The AUC$_t$ of PxLN-H was 3.4-fold greater than that of Taxol$^{(R)}$. PxLN-H gave higher biodistribution in all tissues than did Taxol$^{(R)}$. In addition, it maintained the higher drug concentration for 12 h. This lipid nanosuspension might be a promising candidate for an alternative formulation for the parenteral delivery of poorly water-soluble paclitaxel.

Recovery of C-14 in the Cement Waste Form (농축폐액 시멘트 고화체로부터 C-14 회수 특성)

  • Ahn Hong-Joo;;Lee Jeong-Jin;Pyo Hyung-Yeal;Han Sun-Ho;Jee Kwang-Young
    • Proceedings of the Korean Radioactive Waste Society Conference
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    • 2005.06a
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    • pp.284-289
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    • 2005
  • According to the nuclear safety regulation policy including the administration of radionuclides in low level radwastes, the evaporator bottoms were mixed with cement to form a stable solidification for identifying the recovery possibility of the C-14. The chemical oxidation method was applied for the extraction of C-14 from the cement waste form. The emitting beta ray of the C-14 extracted from the radwastes was measured with the liquid scintillation counter and calculated by using the quenching correction curves. Only the beta emitting radioactive nuclides of the C-14 in the radwastes was showed the radioactivities with the range of $2.7E+00\;{\sim}\;3.07E+02$ Bq/g.

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Numerical Study on the Thermal Control Device for Satellite Components Using the Phase Change Material Combined with Heat Pipe in Parallel (상변화물질과 열관을 병렬 조합한 위성부품 열제어장치의 수치해석적 연구)

  • Shin, Yoon Sub;Kim, Tae Su;Kim, Taig Young;Seo, Young Bae;Seo, Jung-gi;Hyun, Bum-Seok;Cheon, Hyeong Yul
    • Journal of the Korean Society for Aeronautical & Space Sciences
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    • v.44 no.4
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    • pp.373-379
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    • 2016
  • The thermal control device for the periodic working component combined solid-liquid phase change material (PCM) with heat pipes is designed and numerically studied. Due to high latent heat and retaining constant temperature during melting process the component peak temperature, not withstanding small radiator size, is reduced. The warm-up heater power consumption to keep the minimum allowed temperature is also cut down since the accumulated thermal energy is released through the solidification. The thermal buffer mass (TBM) made of Al can give the similar effect but the mass and power consumption of warm-up heater should increase compared to PCM. The amount of PCM can be optimized depending on the component heat dissipation and on/off duty time.