• Journal of the Korea Organic Resources Recycling Association
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• v.10 no.4
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• pp.86-95
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• 2002

Anaerobic digestion of municipal solid waste(MSW) is recently getting attention due to energy generation and abatement of global warming. MSW has high solid content and low nitrogen content. Its major component is cellulose and hemicellulose. The conversion rate of organic portion of MSW to methane is approximately 50%, representing $0.2m^3/kg$ VS. Long hydraulic retention time is required for high solid content and inoculum should be mixed with the feed. When MSW is digested anaerobically, maximum limit of C/N ratio is 25 and the optimum concentration of $NH_3-N$ is 700mg/L. lime and sodium bicarbonate are used to adjust pH. Excess addition of sodium bicarbonate above 3,500mg/L will cause sodium toxicity. Thermophilic anaerobic digestion is effective in the control of pathogen although its operation and maintenance is difficult. To optimize the anaerobic digestion of MSW, it is necessary to understand the mechanism of microorganims involved in anaerobic digestion.

• KSBB Journal
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• v.18 no.6
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• pp.500-505
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• 2003

‘LK (lipoprotein kringle) 68’is a polypeptide of a modified ansiostatin consisting of three kringle structures that might be clinically useful as a potential cancer therapeutics. It can be produced by overexpressing it as inclusion body in recombinant E. coli. In this study, solid-phase refolding processes using packed bed adsorption (PBA) and expanded bed adsorption (EBA) column were carried out to compare their refolding yields with that of the conventional, solution-phase refolding process, For the solution-phase and the PBA-mediated processes employing Q-Sepharose, washed inclusion body was used as the starting material, whereas both washed inclusion body and E. coli homogenate were used for the EBA-mediated process employing streamline DEAE. On the final recovery LK68 per unit mass of wet cell basis, the EBA- and PBA-mediated processes showed about 2.7- and 1.5-fold higher yields, respectively, than the solution-phase refolding method. The solid-phase refolded LK68 demonstrated the same Iysine binding bioactivity and the retention time in the RP-and SEC-HPLC as those of the native protein.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.30 no.3
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• pp.334-339
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• 1997

The feasibility of foam separation to remove solid produced from fish culture water was investigated. Performance characteristics of foam separator were highly dependent upon the operating parameters which were superficial air velocity, Hydraulic retention time (HRT), and foam height. About $50\%$ of the total protein contained in a sample of fish culture water could be removed by foam separator. The removal efficiencies of protein, T-N, TA, and solid components were increased with increasing superficial air velocity and HRT. The combined effects of these operational variables show that removal rates of TVS increase with increasing superficial air velocity and HRT, and decrease as foam height goes up. It could be confirmed that foam separator might offer good perspective for removal of harmful components such as TA and TVS in aquacultural recirculating water.

• Korean Journal of Oriental Medicine
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• v.15 no.2
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• pp.119-124
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• 2009

The purpose of this study was investigation of quantitative analysis of marker substances in Paeonia lactiflora extracts by solid fermentation. High performance liquid chromatography (HPLC) for the determination of albiflorin and paeoniflorin in P. lactiflora extracts by solid fermentation, the separation method was performed on C18 column ($250\;mm\;{\times}\;4.6\;mm$, $5\;{\mu}m$, RS tech) using gradient solvent mixtures of water-acetonitrile with photodiode array detector (230nm). The flow rate was 1.0 ml/min. Retention time of albiflorin and paeoniflorin was about 28.88, 31.92 min and linearity of calibration was showed good result(r2 = 0.9998, 0.9996), respectively. Content of albiflorin was $0.090\;{\pm}\;0.03%$ in P. lactiflora extract(control), $0.102\;{\pm}\;0.00%$ in P. lactiflora extract fermented with Paecilomyces japonica, $0.056\;{\pm}\;0.01%$ in P. lactiflora extract fermented with Ganoderma lucidum, $0.093\;{\pm}\;0.00%$ in P. lactiflora extract fermented with honey and $0.046\;{\pm}\;0.00%$ in P. lactiflora extract fermented with Nuruk. Content of paeoniflorin was $4.506\;{\pm}\;0.13%$ in control, $2.599\;{\pm}\;0.04%$ in P. lactiflora extract fermented with Paecilomyces japonica, $1.222\;{\pm}\;0.03%$ in P. lactiflora extract fermented with Ganoderma lucidum, $2.750\;{\pm}\;0.05%$ in P. lactiflora extract fermented with honey and $0.847\;{\pm}\;0.00%$ in P. lactiflora extract fermented with Nuruk, respectively. Content of the marker substances did not increase in all fermentation experiment group.

• Korean Journal of Oriental Medicine
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• v.16 no.1
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• pp.173-178
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• 2010

The purpose of this study was investigation of quantitative analysis of marker substances in solid fermented Angelicae Gigantis Radix by High performance liquid chromatography(HPLC). HPLC was performed for determination of nodakenin and decursin in solid fermented Angelicae Gigantis Radix extract, the separation method was performed on C18 column ($250\;mm\;{\times}\;4.6\;mm$, $5\;{\mu}m$, RS tech) using gradient solvent mixtures of water-acetonitrile with photodiode array detector (330 nm). The flow rate was 1.0 ml/min. Retention time of nodakenin and decursin was about 11.47, 46.79 min and linearity of calibration was showed good result(r2=0.9999, 0.9999), respectively. Content of nodakenin was $0.76\;{\pm}\;0.02%$ in control, $0.31\;{\pm}\;0.00%$ in Angelicae Gigantis Radix extract fermented with Paecilomyces japonica(SDT)(p<0.01), $0.51\;{\pm}\;0.02%$ in Angelicae Gigantis Radix extract fermented with Ganoderma lucidum(SYT)(p<0.01), $0.82\;{\pm}\;0.03%$ in Angelicae Gigantis Radix extract fermented with honey(SST)(p<0.05) and $0.88\;{\pm}\;0.01%$ in Angelicae Gigantis Radix extract fermented with Nuruk(SNT)(p<0.01). Content of decursin was $4.50\;{\pm}\;0.08%$ in control, $2.90\;{\pm}\;0.05%$ in Angelicae Gigantis Radix extract fermented with Paecilomyces japonica(SDT)(p<0.01), $2.65\;{\pm}\;0.08%$ in Angelicae Gigantis Radix extract fermented with Ganoderma lucidum(SYT)(p<0.01), $4.46\;{\pm}\;0.11%$ in Angelicae Gigantis Radix extract fermented with honey(SST) and $4.73\;{\pm}\;0.04%$ in Angelicae Gigantis Radix extract fermented with Nuruk(SNT)(p<0.05), respectively.

• Bulletin of the Korean Chemical Society
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• v.32 no.9
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• pp.3333-3337
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• 2011

$ZnMn_2O_4$ has been prepared by a mechanochemical process using a mixture of $Mn_2O_3$ and ZnO as starting materials, and investigated as a possible anode material for lithium-ion batteries. The phase evolution and morphologies of the ball-milled and annealed powders are characterized by X-ray diffraction (XRD) and scanning electron microscopy (SEM) with energy dispersive microanalysis (EDX), respectively. The solid-state reaction for the formation of $ZnMn_2O_4$, under the given experimental conditions, is achieved in a short time (30 min), and the prepared samples exhibit excellent electrochemical performances including an enhanced initial coulombic efficiency, high reversible capacity, and stable capacity retention with cycling.

• Journal of environmental and Sanitary engineering
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• v.16 no.1
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• pp.34-39
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• 2001

Submerged Membrane Bio-Reacter(SMBR) process was used to food wastewater treatment. From laboratory pilot-scale experiment data, it was confirmed that this process was very effective process for organics, suspended solid, and N, P treatment. It was found that BOD and COD removal rate were obtained 90% and 92%, respectively, for 150 days operation. Organics loading rate did not affect to the removal efficiency because MLSS concentration in aerobic tank was highly maintained. IN the case of first reactor operated with anoxic and second reactor operated as aerobic, T-N, T-P removal rate were obtained 93% and 95%, respectively. It was shown that removal efficiency could be maintained stable due to the complete removal of SS and sludge production decreased with increasing of sludge retention time.

• Journal of Korean Society of Water and Wastewater
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• v.10 no.2
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• pp.117-123
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• 1996

A bench-scale continuous activated sludge system was operated in order to study the effects of solid waste landfill leachate on municipal wastewater treatment. The leachate sample was collected from Nanjido landfill. During the 7 weeks of operational period, the leachate content in the influent fed to the system was increased stepwise from 3% to 5% and 7%. The solids retention time was the major process control parameter, and it was 10 days. With the municipal wastewater alone, COD removal was 75%. The removal percents, however, decreased to 60% and 45% when the leachate content was 3% and 7%, respectively. For the wastewater spiked with the leachate, the sludge production was higher than for the municipal wastewater alone. Sludge settleability determined by SVI deteriorated with the increase of leachate content. The specific oxygen uptake rate, however, was measured higher as leachate content increased.

• Journal of Industrial Technology
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• v.39 no.1
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• pp.21-25
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• 2019

This work experimentally determined the effect of microaerobic condition on anaerobic digestion of thickened waste activated sludge in semi-continuous mesophilic digesters at hydraulic retention time of 20 days. The concentration of hydrogen sulfide was $7{\pm}2ppm$ at the microaerobic condition and $14{\pm}2ppm$ at the anaerobic condition. Removal efficiency of volatile solid was not significantly different between microaerobic ($40{\pm}8%$) and anaerobic ($38{\pm}8%$) conditions. There was no important difference between microaerobic ($1,352{\pm}98ml/d$) and anaerobic ($1,362{\pm}104ml/d$) conditions in the biogas production, either. Therefore, it could be concluded that the application of the microaerobic condition was an efficient method of the hydrogen sulfide removal from the biogas.

• Journal of Ecology and Environment
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• v.42 no.4
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• pp.191-198
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• 2018

Improper management and unsanitary approaches are implemented in disposal of leachate, which has resulted in groundwater pollution at village Uruli Devachi, Pune, India. Various physico-chemical treatment methods are commercially available for leachate treatment. However, the application of biological methods viz. phytoremediation to the municipal solid waste landfill leachate has been limited. We report the remediation ability of Typha aungstifolia and Acrorus calamus that is capable of reducing hazardous constituents from the landfill leachate. After 96 h of hydraulic retention time (HRT), it was observed that T. aungstifolia-treated sample showed high reduction potential in reducing biochemical oxygen demand, chemical oxygen demand, hardness, total dissolved solids, Na, Mg, Ca and Ni whereas A. calamus showed greater reduction capacity for alkalinity, Cl, Cu, Zn and Cr. Furthermore, it was also observed that T. aungstifolia withstood longer HRT than A. calamus. In situ application of T. aungstifolia and A. calamus for remediation of landfill leachate carries a tremendous potential that needs to be further explored.