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Heterogeneous Natures of the Microbial Steroid $9{\alpha}$-Hydroxylase in Nocardioforms

  • Kang, Hee-Kyoung;Lee, Sang-Sup
    • Archives of Pharmacal Research
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    • v.20 no.6
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    • pp.519-524
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    • 1997
  • Steroid $9{\alpha}$-hydroxylase is an enzyme found in nocardioform microorganisms which can utilize steroids as a sole carbon source. After fractional centrifugation of the cell homogenates, the enzyme activity in Nocardia and Rhodococcus was found in cytoplasmic membrane fraction. On the contrary, Mycobacterium had its 9.alpha.-hydroxylation activity in cytosolic fraction. To characterize the enzyme in these microorganisms, several potential inhibitors of 9.alpha.-hydroxylase were tested and the cofactor requirement for the same enzyme was also examined. The inhibitory effect of ferrous ion chelators indicated involvement of iron containing proteins in the 9.alpha.-hydroxylase system. On the other hand, metyrapone, an inhibitor known to be specific for cytochrome P450 interfered with the enzyme in Mycobacterium, but didn't inhibit the enzyme activity in Nocardia and Rhodococcus. While the $9{\alpha}$-hydroxylase system in Nocardia and Rhodococcus required NADPH, NADH was required as an election donor in Mycobacterium.

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Studies in Remote Functionalization (Ⅰ). Synthesis and Spectroscopic Studies of 3$\alpha$, 5$\alpha$-Cyclosteroidal Substrates

  • Lee, Eun;Park, Sang-Kyu;Lee, Hee-Yoon;Kim, Wan-Joo
    • Bulletin of the Korean Chemical Society
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    • v.2 no.3
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    • pp.105-112
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    • 1981
  • Various $3{\alpha}$, $5{\alpha}$-cyclocholestan-6${\beta}-yl$ ethers were synthesized from solvolysis reactions of cholesteryl tosylate. ${3\alpha}$, $5{\alpha}$-Cyclocholestan-6${\beta}-yl$ sulfides were the sole products when cholesteryl tosylate was solvolyzed in thiol solvents. Diol solvolysis products were derivatized to aromatic esters, and nuclear magnetic resonance spectroscopic method was used to show that aromatic rings can approach C-18 methyl group and the side chain.

First Record of the Genus and Species, Entomognathus brevis (Hymenoptera: Crabronidae: Crabroninae) in Korea

  • Kim, Jeong-Kyu
    • Animal Systematics, Evolution and Diversity
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    • v.35 no.4
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    • pp.191-194
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    • 2019
  • The genus Entomognathus Dahlbom 1829 is recorded for the first time in the Korean Peninsula, represented by the species Entomognathus brevis (Vander Linden, 1829). This species is trans-Palearctic in distribution ranging from northern Africa, through Europe, eastward to the Far East, and sole representative of the genus in the Far East. Of which occurrence in the Russian Far East, northern China and Japan were well documented, but nothing has been known so far in the Korean Peninsula. In this study, distributional range of E. brevis is expanded to South Korea in the Far East. Taxonomic information of the species and a revised key to genera of the tribe Crabronini occurring in the Korean peninsula are given.

Effect of HF and Plasma Treated Glass Surface on Vapor Phase-Polymerized Poly(3,4-ethylenedioxythiophene) Thin Film : Part I

  • Lee, Joonwoo;Kim, Sungsoo
    • Journal of Integrative Natural Science
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    • v.6 no.4
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    • pp.211-214
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    • 2013
  • In this study, in order to investigate how consecutive treatments of glass surface with HF acid and water vapor/Ar plasma affect the quality of 3-aminopropyltriethoxysilane self-assembled monolayer (APS-SAM), poly(3,4-ethylenedioxythiophene) (PEDOT) thin films were vapor phase-polymerized immediately after spin coating of FeCl3 and poly-urethane diol-mixed oxidant solution on the monolayer surfaces prepared at various treatment conditions. For the film characterization, various poweful tools were used, e.g., FE-SEM, an optical microscope, four point probe, and a contact angle analyzer. The characterization revealed that HF treatment is not desirable for the synthesis of a high quality PEDOT thin film via vapor phase polymerization method. Rather, sole treatment with plasma noticeably improved the quality of APS-SAM on glass surface. As a result, a highly dense and smooth PEDOT thin film was grown on uniform oxidant film-coated APS monolayer surface.

A NEW BOOK: 'LIGHT-WATER REACTOR MATERIALS'

  • OLANDER DONALD R.;MOTTA ARTHUR T.
    • Nuclear Engineering and Technology
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    • v.37 no.4
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    • pp.309-316
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    • 2005
  • The contents of a new book currently in preparation are described. The dearth of books in the field of nuclear materials has left both students in nuclear materials classes and professionals in the same field without a resource for the broad fundamentals of this important sub-discipline of nuclear engineering. The new book is devoted entirely to materials problems in the core of light-water reactors, from the pressure vessel into the fuel. Key topics deal with the $UO_2$ fuel, Zircaloy cladding, stainless steel, and of course, water. The restriction to LWR materials does not mean a short monograph; the enormous quantity of experimental and theoretical work over the past 50 years on these materials presents a challenge of culling the most important features and explaining them in the simplest quantitative fashion. Moreover, LWRs will probably be the sole instrument of the return of nuclear energy in electric power production for the next decade or so. By that time, a new book will be needed.

Threonine Production by A Newly Isolated and Characterized Methylotrophic Bacterium (새로운 메탄올자화세균에 의한 트레오닌의 생산)

  • Kim, Kyoung-Ja;Park, Kui-Lea
    • YAKHAK HOEJI
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    • v.36 no.4
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    • pp.315-320
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    • 1992
  • The amino acid threonine was produced from glycine and ethanol in a reaction mixture using resting cells of a newly isolated gram-negative methylotrophic bacterium, capable of growth on methanol. The isolate could utilize $C_1$ compounds and a variety of multicarbon substrates as sole carbon and energy source. To obtain cells of isolate with high threonine producing activity, we investigated optimum cultural conditions. Optimal growth was at the initial concentration of 0.5%(v/v) methanol, at $30^{\circ}C$ and pH 7.0. The growth was not affected by antibiotics inhibiting cell wall synthesis, but was completely suppressed by those inhibiting protein synthesis. The optimum reaction conditions from threonine production by resting cells of this strain were found.

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Simulation of Child Care for First-time Father

  • Jang, Sin-young
    • International journal of advanced smart convergence
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    • v.8 no.2
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    • pp.47-55
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    • 2019
  • In past patriarchal societies, childbearing was considered the sole possession of women. At a time when women were struggling to move into society, the concept of parenting as the mainstay of the capitalist economic society and the head of the family has naturally been taken for granted by a woman named "housewife." Since the role of male babies is as important as that of females, Fathers are trying to promote the importance of the effects of fathers due to active participation in childcare and help change old perceptions of the past. Men also know the importance of participating in childcare in early childhood, but often do not know what their children want or why they cry due to lack of basic child care knowledge and lack of education. We tried to give fathers the meaning of indirect experience and change their perception of parenting by producing interactive VR content, which is completed with dad's participation, so that they can experience the child in person. In addition, through familiar childcare professional product advertisement and 360 degree stereo sound. It is made to immerse in the game to gain persuasive effect, inducing fathers to have interest and interest in childrearing.

Pollination of Cleisostoma scolopendrifolium (Orchidaceae) by megachilid bees and determinants of fruit set in southern South Korea

  • Son, Hyun-Deok;Im, Hyoung-Tak;Choi, Sei-Woong
    • Journal of Ecology and Environment
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    • v.43 no.1
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    • pp.9-13
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    • 2019
  • We investigated the pollinators of Centipede's foot orchid (Cleisostoma scolopendrifolium) at five locations in southern Korea. Only one species of megachilid bee (Megachile yasumatsui) was observed at three of the sites (Mokpo, Haenam, and Wando). We assumed that the megachilid bee worked as a sole pollinator of C. scolopendrifolium based on the visiting behavior of the bees the attached pollinia. Fruits were observed at the three locations that bees visited: 74% fruit set at Mokpo, 59% at Wando, and 31% at Haenam. By contrast, at two of the locations where megachilid bees did not visit the plants (Naju and Jindo), there were no fruits set. The differences in fruit set rates at the three orchid populations where bees were observed appear to be related to bee abundance and flower visitation rate rather than to differences in flower abundance. The pollination interaction between C. scolopendrifolium and megachilid bees appears to involve sexual deception since only male bees were attracted to the flowers. The underlying mechanism involved in this interaction needs investigation.

Skybridges: A History and a View to the Near Future

  • Wood, Antony;Safarik, Daniel
    • International Journal of High-Rise Buildings
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    • v.8 no.1
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    • pp.1-18
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    • 2019
  • As many architects and visionaries have shown over a period spanning more than a century, the re-creation of the urban realm in the sky through connections between buildings at height has a vast potential for the enrichment of our cities. To many it seems nonsensical that, although the $20^{th}$ and now $21^{st}$ century, have clearly seen a push towards greater height and urban density in our major urban centers, the ground-pavement level remains almost exclusively the sole physical plane of connection. As the world rapidly urbanizes, greater thought needs to be expended on how horizontal space can be developed at height. This paper briefly describes the history, present classifications and uses, and potential future development potential of skybridges between tall buildings.

Successful management of systemic lupus erythematosus with levamisole in a Dachshund dog

  • Kim, Dong-Hyun;Han, Hyun-Jung;Kim, Jung-Hyun
    • Korean Journal of Veterinary Research
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    • v.61 no.1
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    • pp.1.1-1.5
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    • 2021
  • A 5-year-old neutered female Dachshund dog presented with a 3-month history of hyperthermia, skin lesions, and shifting lameness. Based on physical examination, blood tests, urinalysis, and radiographs, the dog was diagnosed with systemic lupus erythematosus. Clinical signs improved after administration of prednisolone and cyclosporine but relapsed after the prednisolone was reduced due to side effects. Oral levamisole was commenced and the other immunosuppressants were tapered over a period of 2 months and then stopped. Levamisole was retained as the sole therapy for an additional 2 months. Six months after discontinuation of all treatment, the patient remained in remission.