• Title/Summary/Keyword: Solamum tuberosum L.

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Culture and Fusion of Protoplasts from Potato (Solanum tuberosum L.) and Tobacco (Nicotiana tabacum L.) (감자(Solanum tuberosum L.)와 담배 (Nicotiana tabacum L.)의 원형질체 배양 및 융합)

  • 정상호
    • Journal of Plant Biology
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    • v.30 no.4
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    • pp.287-298
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    • 1987
  • The regenerative capacities of protoplasts isolated from potato (Solamum tuberosum L.) tubers and tobacco (Nicotiana tabacum L.) mesophyll tissues were examined, and then their intergeneric protoplast fusion was carried out. The potato tuber-derived protoplasts proliferated into the calli some of which showed rudimentary shoot-like structures, which had not been attempted before from tubers, while the tobacco protoplasts were regenerated into the whole plants. Intergeneric protoplast fusion between potato and tobacco was carried out and the heteroplasmic fusion products were formed. The first cell division of some of them was observed after 5 days of culture.

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Effects of Culture Type and Inoculation Quantity in Bioreactor on Production of Potato Plantlets

  • Choi Ki Young;Son Sung Ho;Lee Joo Hyun;Lee Yong-Beom;Bae Jong Hyang
    • Journal of Bio-Environment Control
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    • v.14 no.4
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    • pp.298-301
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    • 2005
  • Potato (Solamum tuberosum 'Dejima') plantlets were investigated on culture type and initial quantity of inoculation in bioreactor and survival rate by hydroponics for mass production. rode stems (1 to 1.5cm in length) of potato plantlets multiplied in vitro were grown for 3 weeks in liquid Murashige and Skoog (MS) medium with sucrose $30 g\; L^{-1}$. When plantlets (80-node inoculation) were raised in 10L balloon type bubble (BB) bioreactor, the healthiest growth of plantlets was obtained from explants cultured in ebb & flow culture with medium supplied periodically 12 times per day. The suitable inoculation quantity of 20L BB bioreactor was 120 pieces of stem segments (mean 2.2g fresh weight) in ebb & flow culture. Number of nodal shoot was eight on the average. In controlled culture room, survival rate of plantlets at 7 days after stem cutting was above $70\%$ when they were acclimatized by hydroponics grown in deep flow and solid medium culture. The highest survival rate of the stem cutting plantlets was in nutrient solution adjusted to EC $1.4dS{\cdot}m^{-1}$. Stem cutting plantlets through one culture could be obtained $670\~900$, when plantlets were grown in ebb & flow culture during 3 weeks using a 20L bioreactor with initial 120 pieces of nodal segments. 11 is possible In do mass production of seedlings cultured in bioreactor and hydroponics.

Quality and Yield Characteristics of Potato (Solanum tuberosum L.) Grown at Paddy Field in Spring Season

  • Im, Ju Sung;Cho, Ji Hong;Chang, Dong Chil;Jin, Yong Ik;Park, Young Eun;Chun, Chung Gi;Kim, Dong Un;Yu, Hong Seob;Lee, Jong Nam;Kim, Myung Jun
    • Horticultural Science & Technology
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    • v.31 no.1
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    • pp.24-31
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    • 2013
  • This study was conducted to determine the characteristics of quality and yield in potatoes grown at paddy field before rice transplantation during the spring season. Three potato cultivars ('Jowon', 'Haryeong', and 'Goun') were grown in Gangneung (asl 5 m) and Seocheon (asl 20 m). In both locations, weather condition belonged to the fourth zone (spring cropping) in potato production location's distribution of Korea. Daily mean soil temperature in both the locations was $0.2-0.6^{\circ}C$ lower than air temperature, while soil moisture was adequate level to potato growth in spite of spring drought. TR ratio was not affected by location, but by cultivar. Specific gravity, starch content, dry matter rate, and yield were significantly influenced by location and by cultivar. There was no difference in total tuber number by location, however there was a large gap in marketable tuber yield according to locations and cultivars. There were high negative relationships between yield and main qualities such as dry matter rate and starch content, while high positive correlation was observed between main qualities. It was possible to produce potato before rice transplanting at drained paddy fields located in representative two locations of potato spring cropping and their characteristics in growth and quality were similar to those generally well known in upland cultivation. Paddy field was thought to be more favorable than upland in terms of available soil moisture supply against spring drought. Further research, however, was needed to increase soil temperature and also preliminary review on proper cultivar according to location seemed to be needed for high yield.

Mechanization for Labor Saving in Harvesting of Potatoes (Solamum Tuberosum L.) (감자 수확의 성력 기계화)

  • 정동희
    • Korean Journal of Plant Resources
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    • v.8 no.3
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    • pp.297-302
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    • 1995
  • The experiment is conducted to reduce the labor and production cost with the labor save of harvest if cultivating the potatoes using the machine and the results are as follows. On labor saving effect in transparent vinyl mulching, digging working hours per 10a in the case of using tractor are 60 min., fixing + turning time is 5 min. and 30sec., the time of harvest is 65 min and 30sec., digging working hours using cultivator are 99 min. and fixing +turning time is 5 min. and 30sec., but the time of hand harvesting is 990 min. and in digging labor saving effect, tractor shows 94% in the harvesting period and harvest by cultivator 90%. On non mulching cases, the harvest by tractor takes 49 min. and 30 sec. and that by cultivator does 87 min. and 30 sec., and digging labor saving effect shows 94% in the tractor harvest and 90% in the cultivator harvest. Therefore, on the operation efficiency per hour, in the case of tractor with digger vinyl mulching and non mulching show, $0.091\sim0.121ha$ and in the case of cultivator with digger both show $0.057\sim0.069ha$, so in the mechanized harvest of potatoes, the harvest by tractor with digger is the best. On the cost and labor save for harvesting the potatoes with labor saving effect, tractor shows 19 hours and 20 min./10a in vinyl mulching and 19 hours. and 54 min./10a in non mulching, so it shows the short hours for harvesting. And labor saving effect shows $42\sim45%$ in comparison with 35 hours and 21 min. $\sim35$ hours and 23 min.($123,113\sim130,613$ won) of the hand harvest, so the cost was reduced to $71,250\sim72,225$ won. On the cultivator with diggers, vinyl mulching takes 19 hours and 55 min. and non mulching 19 hours and 38 min., so the labor saving effect and cost were reduced to $14\sim44%$ ($72,675\sim73,313$ won) in comparison with the hand harvest.

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