• Journal of Microbiology and Biotechnology
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• v.24 no.1
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• pp.113-118
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• 2014

Environmental microorganisms are emerging as an important source of new enzymes for wide-scale industrial application. In this study, novel phytase genes were identified from a soil microbial community. For this, a function-based screening approach was utilized for the identification of phytase activity in a metagenomic library derived from an agricultural soil. Two novel phytases were identified. Interestingly, one of these phytases is an unusual histidine acid phosphatase family phytase, as the conserved motif of the active site of PhyX possesses an additional amino acid residue. The second phytase belongs to a new type, which is encoded by multiple open reading frames (ORFs) and is different to all phytases known to date, which are encoded by a single ORF.

• Korean Journal of Soil Science and Fertilizer
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• v.6 no.4
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• pp.227-230
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• 1973

To elucidate the effect of the organic carbon compounds included in a separate from glutamic acid fermentation residue (G. A. F. R) on the improvement of the physico-chemical properties of soil, on a soil low in organic matter content, treated with G. A. F. R and compost, observations on the total organic matter, humic acid, fulvic acid, C. E. C. and the development of aggregates were made. From the results of the investigations it was concluded that, the organic carbon compound in the tested G. A. R. F. is more effective than compost in increasing the total organic matter, humic acid, fulvic acid and C. E. C. of soil and in enhancing the development of soil aggregates.

• Korean Journal of Environmental Agriculture
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• v.9 no.1
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• pp.15-22
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• 1990

The efects of application time on the persistence of chlorothalonil in sesame plants and its persistence in soil were studied in the field. A procedure was used which permited the analysis of residues of Chlorothalonil (DACONIL 2787) in sesame(Sesamum indicum L.)and soil. The procedure was based on the multiresidue analytical method using an polarized acetonitrile solution or acidified acetone as the extracting solvent. The DACONIL 2787 is separated from the fat in the extracts by using an activated alumina and Florisil chromatogaphic column. Residues of DACONIL 2787 were determined by subjecting the alternate eluate from the Florisil column to gas chromatographic analysis(ECD). Residue of chlorothalonil in sesame are proved to 0.06 ppm for six sprays, 3rd day before harvest The half-life of chlorothalonil in soil proved to be 8.9 days and residues of chlorothalonil was only 0.03 ppm 100 days after application of 15 ppm.

• Korean Journal of Soil Science and Fertilizer
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• v.16 no.2
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• pp.156-161
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• 1983

In order to find out the effect and the optimum application amount of special organic fertilizer which is granuated with fermentation residue of glutamate two field experiments were conducted with corn and chinese cabbage. The results were summarized as follows: 1. The yields of corn and chinese cabbage were slightly increased with the application of special organic fertilizer. 2. There was fertilizer injury in chinese cabbage but not in corn when applied 300kg/10a after application of standard fertilizer levels for crops with general chemical fertilizers. 3. The absorption amount of total sugar in corn plant and content of vitamin E in chinese cabbage leaves after the harvest were increased with the application of special organic fertilizer. 4. In the soil where the special organic fertilizer was used, the organic matter content after experiment increased slightly but the tendency was not constant. 5. According to the increase of the special organic fertilizer application level, the soil pH after experiment decreased. This tendency was more clear at corn cultivated area.

• Korean Journal of Organic Agriculture
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• v.32 no.1
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• pp.107-125
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• 2024

Three types of veterinary antibiotics, including oxytetracycline (OTC) and chlortetracycline (CTC) of tetracycline class and amoxicillin (AMX) of penicilline class, were artificially introduced into the irrigation water. The residue of veterinary antibiotics in the soil, the absorption-translocation of veterinary antibiotics into the red lettuce and young radish plant, and crops yield were investigated according to the agricultural water irrigation method (surface drip irrigation, underground drip irrigation, and sprinkler irrigation). There was no significant difference in the residue and translocation of veterinary antibiotics in the soils and crops according to the irrigation method and type of veterinary antibiotics (p>0.05). For the edible parts of red lettuce and young radish, all three types of veterinary antibiotics were found to be below the detection limit, indicating that the safety of the crops was secured. The translocation factor of red lettuce and young radish were found to be less than 0.3 and 0.2, respectively. However, continuous introduction of veterinary antibiotics in agricultural arable lands may have negative effects by affecting soil microbial activity and soil microbe species diversity, so continuous management is deemed necessary.

• Applied Biological Chemistry
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• v.35 no.2
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• pp.115-121
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• 1992

Avermectin B1(abamectin) is an very effective acaricide/insecticide. Because it is applied at low rates, the resultant residue level would be quite low and this requires highly sensitive analytical method. In this study, three analytical methods for avermectin $B1_a$, were compared in view of detectability and sensitivity. The first analytical method was an HPLC method employing the fluorescence detection of avermectin. The second analytical technique to quantitate avermectin $B1_a$, and its photodegradative delta 8,9-isomer employed trifluoroacetic anhydride and 1-methylimidazole in DMF. The new method was the modification of trifluorescence method. The average recoveries of avermectin $B1_a$ for the concentration range from 1 and 10 ng/g in whole apple fruit by fluorescence method were 90.3% and 88.2% respectively. In trifluorescence method, the recoveries of the avermectin $B1_a$ and delta 8,9-isomer were 100.7% and 94.7% in concentration from 5 ng/g and 25 ng/g. The average recoveries of 5 ng and 25 ng/g in the newly modified method were 95.0, 99.0. 96.0, 92.8% in whole apple and soil respectively.

• Korean Journal of Soil Science and Fertilizer
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• v.43 no.6
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• pp.911-916
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• 2010

Rice paddy fields and crop residue burning are a major source of methane ($CH_4$) emissions, a potent greenhouse in agriculture. This study was conducted to assess $CH_4$ emissions in Korea cropland sector from 1990 to 2008. Greenhouse gas emissions from the cropland sector are calculated in two categories: 4C (Rice cultivation) and 4F (Field burning). In 4C: Rice Cultivation, methane emissions from paddy fields (continuously flooded and intermittently flooded) cultivated for rice production had decreased from 395 $CH_4$ $10^3$ Mg in 1990 to 297 $CH_4$ $10^3$ Mg in 2008. $CH_4$ emissions converted into $CO_2$ equivalent were 8,303 $CO_2$-eq. $10^3$ Mg in 1990 and 6,229 $CO_2$-eq. $10^3$ Mg in 2008. Greenhouse gas emissions from paddy field in Korea showed that it was gradually going down as the cultivation area decreased. In 4F: Field Burning, methane emissions by burning crop residue increased from 2,502 $CH_4$ Mg in 1990 to 2,726 $CH_4$ Mg in 2008. Emissions converted $CH_4$ into $CO_2$ equivalent were 53 $CO_2$-eq. $10^3$ Mg in 1990 and 57 $CO_2$-eq. $10^3$ Mg in 2008. Total emissions of $CH_4$ from the cropland sector declined from 8,356 $CO_2$-eq. $10^3$ Mg in 1990 to 6,287 $CO_2$-eq. $10^3$ Mg in 2008.

• The Korean Journal of Pesticide Science
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• v.17 no.3
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• pp.168-177
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• 2013

This experiment was carried out to estimate leaching potential of thirteen endocrine disruptor-suspected pesticides in upland soils using soil columns (5 cm I.D. ${\times}$ 35 cm H.) packed with soil A (sandy loam) and soil B (loam). When 12.6 mL of water, average precipitation in Cheongju area during the period from June to August, 2001-2010, was percolated through soil column packed with soil A every day for 21 days, no pesticides were detected from leachate, with the exception of metribuzin which was detected with negligible. Also, when 2 L of water was percolated consecutively five times through soil columns packed with soil A and B, irrespective of soil types, cypermethrin, endosulfan, fenvalerate, parathion and trifluralin, which were very low water solubilities and high soil $K_{oc}s$, were not detected from leachate and were distributed mostly in the depth of 0-5 cm, representing that water solubility and soil $K_{oc}$ are major contributing factors to their leaching behavior. Despite high average leaching rates in carbaryl and methomyl, actual possibilities of ground water contamination in the agricultural environment by them would be very low, considering that the negligible amount of pesticide was percolated through a lysimeter with an undisturbed soil core simulating the field conditions, while most of pesticide was percolated through a soil column with the disturbed soil profile.

• Korean Journal of Soil Science and Fertilizer
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• v.47 no.6
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• pp.586-593
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• 2014

Newly reclaimed tidal land is known to be in low status of soil fertility. The incorporation of crop residue is an effective method to improve soil properties and fertility in reclaimed saline soils. The objective of this study was to evaluate the efficiency of rice straw (RS) application to improve physico-chemical properties of saline-sodic soil and its contribution to productivity of whole crop barley. Increasing rate of rice straw improved growth parameter related to yield of whole crop barley, which increased tiller number significantly (p<0.05).The yield increased by 15% (F.W) and 9% (D.W) in rice straw-amended plots. The content of soil organic matter (SOM) in the surface soil (0-20cm) with rice straw incorporation increased by 5~9% (RS 2.5~RS 7.5) compared to RS 0, in which the content of SOM decreased after two consecutive cultivations. Rice straw incorporation promoted soil physico-chemical properties and nutrient-availability of the test crop, as indicated in change in soil bulk density, porosity and increased nutrient uptake of plant. Especially, the P content and uptake of whole crop barley increased with increasing the rate of rice straw application. In conclusion, the rice straw application at rates of $5.0-7.5ton{\cdot}ha^{-1}$ in reclaimed saline soils effectively improved soil properties and crop productivity, which has potentials to reduce the loss of chemical fertilizers and facilitate the favorable condition for crop growth under adverse soil condition.

• Korean Journal of Soil Science and Fertilizer
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• v.28 no.4
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• pp.327-333
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• 1995

The sludge application increased the panicle number per plant and number of spikelets per panicle, resulting in an increase in yield by 10% at Cheongdo, 15% at Chinju and 7% at Yeocheon. With the sludge application (3% w/w), $SiO_2$ content of brown rice was significantly increased. The sludge aplied up to 34.8 ton per hectare increased pH, calcium, and potassium in paddy soils, the application of the sludge to reclaimed saline soil at Yeocheon needs to be reevaluated since the sludge increased soil pH to 7.2 and aggravated drainage.