• Title/Summary/Keyword: Soil nutrient

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Production of Antimicrobial Compounds and Cloning of a dctA Gene Related Uptake of Organic Acids from a Biocontrol Bacterium Pseudomonas Chlororaphis O6 (생물적 방제균 Pseudomonas chlororaphis O6의 길항 물질 생산 및 유기산 흡수에 관련된 dctA 유전자의 클로닝)

  • Han, Song-Hee;Nam, Hyo-Song;Kang, Beom-Ryong;Kim, Kil-Yong;Koo, Bon-Sung;Cho, Baik-Ho;Kim, Young-Cheol
    • Korean Journal of Soil Science and Fertilizer
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    • v.36 no.3
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    • pp.134-144
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    • 2003
  • A rhizobacterium Pseudomonas cholororaphis O6 produced several secondary metabolites, such as phenazines, protease, and HCN that may be involved in inhibition of the growth of phytopathogenic fungi. In field study, P. chlororaphis O6 treatment on wheat seed suppressed root rot disease caused by Fusarium culmorum. The major organic acids of cucumber root exudates were fumaric acid, malic acid, benzoic acid, and succinic acid. Glucose and fructose were major monosaccharides in cucumber root exudates. The total amount of organic acids was ten times higher than that of the sugars. P. chlororaphis O6 grew well on cucumber root exudates. The dctA gene of P. chlororaphis O6 consisted of a 1,335 bp open reading frame with a deduced amino acid sequence of 444 residues, corresponding to a molecular size of about 47 kD and pI 8.2. The deduced dctA sequence has ten putative transmembrane domains, as expected of a membrane-embedded protein. Our results indicated that organic acids in cucumber root exudates may play an important role in providing nutrient source for root colonization of biological control bacteria, and the dctA gene of P. chlororaphis O6 may be an important bacterial trait that is involved in utilization of root exudates.

Response of Soil Properties to Land Application of Pig Manure Liquid Fertilizer in a Rice Paddy (돈분뇨 액비가 시용된 논토양 특성 변화)

  • Kim, Min-Kyeong;Kwon, Soon-Ik;Kang, Seong-Soo;Han, Min-Soo;Jung, Goo-Bok;Kang, Kee-Kyung
    • Journal of the Korea Organic Resources Recycling Association
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    • v.19 no.4
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    • pp.97-105
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    • 2011
  • A wide diversity of liquid fertilizers and composts produced from the livestock manure in Korea is commonly applied to agricultural lands as an alternative of chemical fertilizers. However, their effects on the crop production and environmental impacts are still vague. The current study was investigated the property changes of paddy soils in sandy loam and silty loam treated with 1) control (no treatment), 2) chemicals, 3) storage liquid fertilizer and 4) SCB liquid fertilizer located in Gyeong-gi province, Korea. The chemical properties of soils in sandy loam and silty loam before the treatment were similar with the ones in the average paddy fields in Korea. Contrary to this, the amount of available phosphorus in sandy loam was higher than the one in the average paddy fields. The number of living organisms in sandy loam and silty loam treated with storage liquid fertilizer and SCB liquid fertilizer were higher than the ones in sandy loam and silty loam with no-treatment and chemicals. Significant difference (P<0.05) among the treatments and no-treatment was observed in sandy loam rather than in silty loam. The amounts of heavy metals were the highest in both sandy loam and silty loam treated with storage liquid fertilizer and SCB liquid fertilizer. The comparison of heavy metals showed that the ones in silty loam were little bit higher than sandy loam. The leaf lengths and dry weights of rices were increased over time, however, no significant difference was observed among each treament. In addition, the rice yield in sandy loam treated with SCB liquid fertilizer was higher than the ones in sandy loam. The highest rice yield was obtained from sandy loam treated with chemicals, but there was no significant difference between storage liquid fertilizer and SCB liquid fertilizer. While the rate of nutrient absorption by rices was the highest in sandy loam and silty loam treated with chemicals, there was no significant difference in sandy loam and silty loam treated with livestock liquid manure.

Agronomical studies on the major environmental factors of rice culture in Korea (수도재배의 주요환경요인에 관한 해석적 조사연구)

  • Yung-Sup Kim
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.3
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    • pp.49-82
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    • 1965
  • For the stable and high yields of low-land rice in Korea, the characteristics of rice plant for the vegetative and physiological responses, plant type formation, and yield components have been studied in order to obtain the fundamental data for the improvement of cultural practices, especially for the ideal fertilizer application. Furthermore the environmental conditions in Korea including temperatures, light, precipitation, and soil conditions have been compared in the broad sense with those in Japan, and the application of nitrogen, phosphorus, potassium, silicate and other micro-nutrients were described in relation to the characteristics of environmental conditions for the improvement of fertilizer application. 1. The average yield of polished-rice per 10 are in Korea is about 204 kg and this values are much less than those in Japan and Taiwan where they produce 77% to 13% more than in Korea. The rate of yield increase a year in Korea is 4.2 kg, but in Japan and Taiwan the rates of yield increase a year are 81 % and 62%, respectively. It was also found that the coefficient of variation of yield is 7.7% in Korea, 6.7% in Japan and 2.5% in Taiwan. This means that the stability of producing rice in Korea is very low when compared with those in Japan and Taiwan. 2. It was learned from the results obtained from the 'annual yield estimation experiment' that there are big differences in the respect of plant type formations between rice crops grown in Japan and Korea. The important differences found were as follows: (1) The numbers of spikelets per 3.3 square meters are 891 in Korea and 1, 007 in Japan(13% more than in Korea). (2) The numbers of tillers per 3.3 square meters at the stage of maximum tillering are 1, 150 in Korea, but in Japan they showed 19% more than in Korea. (3) The ratio of effective tillers to total tillers is 77.5% in Korea and 74.7% in Japan, which seems to be higher in Korea than in Japan. But the ratio in Korea is very low when considered the numbers of total tillers in both countries. (4) The ratio of grain to straw is 85.4% in Korea and 96.3% in Japan. 3. The average temperatures during the growing season at the area of Suwon, Kwangjoo and Taegu are almost same as those in the district of Jookokoo(Fookoo yama) in Japan, i.e., the temperatures during the rice-growing season in Korea are similar to those in the southern-warm regions of Japan. 4. Considering the minimum temperatures at the stage of limiting transplanting, 13$^{\circ}C$, the time of transplanting might be 30 to 40 days earlier than presently practicing transplanting time, which comes around June 10. 5. The temperatures during the vegetative growth in Korea were higher than those temperatures that needed in the protein synthesis which ate the main metabolism during this stage. However, the temperatures at the time of reproductive growth was lower than the temperatures that needed in the sugar assimilation which is main metabolism in this stage. In this point of view, it might be considered that the proper time of growing rice plant in Korea would be rather earlier. 6. The temperatures and the day light conditions at the time of first tillering stage of rice plant, when planted as presenting transplanting practices, are very satisfactory, but the poor day light length, high temperatures and too wet conditions in the time of last-tillering stage(mid or last July) might cause the occurrence of disease such as blast. 7. The heading stage of rice plants at each region through nations when planted as presently practicing method comes when the day light length is short. 8. It was shown that the accumulated average air-temperature at the time of maturing stage was not enough and the heading time was too late, when considered the annual deviations of mean temperatures and low minimum temperatures. 9. The nitrogen content of each plant part at the each growing stage was very high at the stage of vegetative growth when compared with the nitrogen content at the stage of reproductive growth after heading. In this respect it was believed to be important to prevent the nutrient shortages at the reproductive stages, especially after the heading. 10. The area of unsatisfactory irrigation paddy fields and natural rain-fed paddy fields are getting reduced in Korea. The correlation between the rate of reducing unsatisfactory irrigation and natural rain-fed paddy fields and the rate of yield increase were computed. The correlation coefficients(r) between the area of unsatisfactory irrigation paddy fields and yield increase were +0.525, and between the natural rain-fed paddy fields and yield increase, +0.832 and between the unsatisfactory irrigation plus natural rain-fed paddy fields and yield increase, +0.84. And there were. highly significant positive correlations between natural rain-fed paddy fields and yield increases indicating that the less the area of natural rain-fed paddy fields, the greater the yields per unit area. 11. The results obtained from the fertilizer experiments (yield performance trials) conducted in both Korea and Japan showed that the yield of non-fertilized plots per 10 are was 231 kg in Korea and 360 kg in Japan. On the basis of this it might be concluded that the fertility of soil in Korea is lower than that in Japan. Furthermore it was. also found that the yields of non-nitrogen applied plots per 10 are were 236 kg in Korea and 383 kg in Japan. This also indicates that the yields of rice in Korea are largely depending on the nitrogen content in the soil. 12. The followings were obtained when the chemical natures of soils in both Korea and Japan were compared. (1) The content of organic matter, total nitrogen, exchangeable calcium, and magnesium in Korea were no more than the half those in Japan. (2) The content of N/2 chloride and soluble silicate in low-land soil were on the average lower in Korea. (3) The exchange capacity of bases in Korea was no more than half that in Japan. 13. It was also observed by comparing the soil nature of the soil with high yielding capacity with the soil with low yielding capacity that the exchange capacity of bases, exchangeable calcium and magnesium, potassium, phosphorus, manganese, silicate and iron were low in the soil with low yielding capacity. 14. The depth of furrow slice was always deeper in the soil with high yielding capacity, and the depth of furrow slice in Korea was also shallower than that in Japan. 15. Summarizing the various conditions mentioned previously and considering the effects of silicate and trace elements such as manganese and iron besides three elements on the physiological and plant type formation of rice crops, more realistic and more ideal fertilizing practices were proposed. proposed.

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Estimation of Rice Cultivation Impacts on Water Environment with Environmental Characteristics and Agricultural Practices by Nitrogen Balances (질소수지에 의한 환경특성과 영농방법별 벼농사의 수질영향 평가)

  • Roh, Kee-An;Kim, Min-Kyeong;Ko, Byong-Gu;Kim, Gun-Yeob;Shim, Kyo-Moon;Lee, Deog-Bae
    • Korean Journal of Soil Science and Fertilizer
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    • v.42 no.6
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    • pp.439-446
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    • 2009
  • Nitrogen balance in the regional scale which was calculated the difference between nitrogen input and output was estimated to assess the impact of rice cultivation on water environment. Nitrogen balances in Gyeonggi province, where nitrogen concentration in irrigation water was high and in Chungnam province, where nitrogen absorbtion by rice was high, were -5.4 and -8.3 kg $-8.3kg\;ha^{-1}\;yr^{-1}$, respectively. Nitrogen balances of paddy field in Gangwon province, where nitrogen output was small and irrigation water was clean, and in Gyeongnam province, where organic matter content of soil was high and rice yield was low, were 4.9 and $14.0kg\;ha^{-1}\;yr^{-1}$, respectively. Average nitrogen balance and total nitrogen absorption of paddy field in Korea were estimated to $-0.3kg\;ha^{-1}\;yr^{-1}$ and $-3,315Mg\;yr^{-1}$, respectively. When the nitrogen concentration in irrigation water was increased by $1mg \;L^{-1}$, nitrogen balance of rice paddy changed by $-2.91kg\;ha^{-1}\;yr^{-1}$. Also, when nitrogen fertilizer applied was decreased from 110 to $90kg\;ha^{-1}$ and the same harvest was maintained, the nitrogen absorption by rice paddy from irrigation water was estimated to increase by 10,600 Mg per year in Korea. However, in cases, the harvest was reduced to either 90% or 85%, nitrogen balances were changed from -11.7 to -2.3 and $2.4kg\;ha^{-1}$, respectively. These results suggest that the reduction of nitrogen fertilizer use may not always lead to a negative nitrogen balance and sustainable agriculture can achieve by not cutting down the use of fertilizer only but by reduction of fertilizer application concurrently by maintenance of harvest and by utilization of environmental characteristics such as nutrient contents in irrigation water and soils.

The Treatment of Night Soil using Bacillus sp. (Bacillus sp.를 이용한 분뇨처리)

  • 염혜경;이은숙;이병헌;이민규;정일호;김중균
    • Journal of Life Science
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    • v.12 no.6
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    • pp.700-707
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    • 2002
  • To study the characteristics of organic and nutrient removal by Bacillus species at high COD concentration of influent, three lab-scale batch reactors(R1, R2, R3), each of which has different substrate composition, were operated. More than 95% of $NH_4^+$-N and $COD_{cr}$, concentrations were removed under an aerobic condition, and their removal efficiencies were found to be 22.6 and 90.5%(R1), 23.9 and 65.8%(R2), 30.2 and 86.4%(R3), respectively. The removal efficiency of $NH_4^+$-N was high when an enough amount of $NO_3^{-}$-N was supplied, and that of $COD_{cr}$. was low when a high concentration of initial $NO_2^{-}$-N was added. The amount of carbon utilized in denitrification was a little. In all reactors,$NO_3^{-}$-N was removed under an anoxic condition, but in the R3 reactor, 10% of $NO_3^{-}$-N could be removed even undo, an aerobic condition. The removal efficiencies of TN and TP were 41.8 and 49.5%(R1), 40.1 and 35.8%(R2), 47.0 and 57.6%(R3), respectively. Alkalinities destructed under an aerobic condition for each reactor were 4.96, 5.41 and 3.93 mg/L (as $CaCO_3$) per each gram of $NH_4^+$-N oxidized, respectively, while 3.06, 3.17 and 2.60 mg/L (as $CaCO_3$) of alkalinities were produced for each gram of ,$NO_3^{-}$-N reduced to $N_2$. The SOUR were found to be 38.5, 52.7 and 42.0 mg $O_2$/g MLSS/hr, which indicated that Bacillus sp. had a higher cell activity than activated sludge. The OLR and sludge production were estimated to be 0.69 and 0.28(Rl), 0.77 and 0.20(R2), 0.61 kg COD/$m^3$/day and 0.25 kg MLSS/kg COD(R3), respectively. From the N-balance, the highest percentage(40.9%) of nitrogen lost to $N_2$ was obtained in the R3 reactor. From all the results, the possibility of aerobic denitrification Bacillus sp. has been shown and the B3 process seemed to have two advantages: a little amount of carbon was required in denitrification and not much amount of alkalinity was destructed under an aerobic condition.

The Effects of 7 Fertilizers on the Growth and Nutrient Concentrations of Fraxinus rhynchophylla, Fraxinus mandshurica, Pinus koraiensis, and Abies holophylla Seedlings (7가지 시비처리가 물푸레나무, 들메나무, 잣나무, 전나무 묘목의 생장 및 양분농도에 미치는 영향)

  • Han, Si Ho;Byun, Jae Kyung;Cho, Min Seok;An, Ji Young;Park, Gwan Soo;Kim, Se Bin;Park, Byung Bae
    • Journal of Korean Society of Forest Science
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    • v.105 no.2
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    • pp.177-185
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    • 2016
  • Fertilization is required to keep the balance of tissue nutrients and to produce high quality seedlings at the permanent nursery. This study was conducted to verify the optimum fertilization method for Fraxinus rhynchophylla, F. mandshurica, Pinus koraiensis, and Abies holophylla seedlings with vector diagnosis method. Seven treatments include nitrogen (N, $13.8g{\cdot}m^{-2}$), phosphorus (P, $6.1g{\cdot}m^{-2}$), potassium ($7.5g{\cdot}m^{-2}$) fertilization and 1x (N $6.9g{\cdot}m^{-2}$, P $3.05g{\cdot}m^{-2}$, K $3.65g{\cdot}m^{-2}$), 2x (twice of 1x), 4x (four times of 1x) fertilization and no fertilization. Soil pH decreased as fertilization increased. Nitrogen and NPK fertilization decreased exchangeable $Ca^{2+}$ and $Mg^{2+}$ concentrations. Height and root collar diameter of F. rhynchophylla and F. mandshurica significantly increased with N and NPK fertilization, but those of P. koraiensis and A. holophylla did not. The biomass of F. rhynchophylla and F. mandshurica was about twice higher at NPK fertilization compared to the control. The responses of vector diagnosis were different by tree species and fertilization treatment: F. rhynchophylla was in the status of N "dilution", which means the N concentration decreases with N content. Phosphorus and K were "sufficiency" state with 4x fertilization. F. mandshurica showed "retranslocation" as N content decreased without change of dry weight at N, P, K fertilization, but "dilution" state at NPK fertilization. This result suggested that optimal fertilization was required for F. rhynchophylla and F. mandshurica in seedling production stage, but was not essential for P. koraiensis and A. holophylla.

Development and Application of the SWAT HRU Mapping Module for Estimation of Groundwater Pollutant Loads for Each HRU in the SWAT Model (SWAT HRU별 지하수 오염부하량 산정을 위한 SWAT HRU Mapping Module 개발 및 적용)

  • Ryu, Ji Chul;Mun, Yuri;Moon, Jongpil;Kim, Ik Jae;Ok, Yong Sik;Jang, Won Seok;Kang, Hyunwoo;Lim, Kyoung Jae
    • Journal of Environmental Policy
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    • v.10 no.1
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    • pp.49-70
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    • 2011
  • The numerous efforts have been made in understanding generation and transportation mechanism of nonpoint source pollutants from agricultural areas. Also, the water quality degradation has been exacerbated over the years in many parts of Korea as well as other countries. Nonpoint source pollutants are transported into waterbodies with direct runoff and baseflow. It has been generally thought that groundwater quality is not that severe compared with surface water quality. However its impacts on groundwater in the vicinity of stream quality is not negligible in agricultural areas. The SWAT model has been widely used in hydrology and water quality studies worldwide because of its flexibilities and accuracies. The spatial property of each HRU, which is the basic computational element, is not presented. Thus, the SWAT HRU mapping module was developed in this study and was applied to the study watershed to evaluate recharge rate and $NO_3-N$ loads in groundwater. The $NO_3-N$ loads in groundwater on agricultural fields were higher than on forests because of commercial fertilizers and manure applied in agricultural fields. The $NO_3-N$ loads were different among various crops because of differences in crop nutrient uptake, amount of fertilizer applied, soil properties in the field. As shown in this study, the SWAT HRU mapping module can be efficiently used to evaluate the pollutant contribution via baseflow in agricultural watershed.

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Effects of Ammonium Sulfate and Potassium Sulfate Fertilizer on Dry Matter Yield and Forge Quality of Sorghum X Sudangrsss Hybrid in Reclaimed Tidal Land (간척지에서 수수 X 수단그라스에 대한 유안 및 황산칼리비료 시용효과)

  • Shin Jae Soon;Lee Seung Heon;Kim Won Ho;Kim Jong Geun;Yoon Sei Hyung;Lim Keun Bal
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.25 no.4
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    • pp.245-250
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    • 2005
  • This experiment was conducted to compare the frech and dry matter yields and feed values of Sorghum$\times$Sudangrsss Hybrid in accordance with different nitrogen and potassium fertilizer sources at the Dae-Ho reclaimed tidal land, Korea from 2003 to 2004. Soil salt contents of ammonium sulfate plots(T3, T4, T5) were higher than that of the urea plot(T2), but that of potassium sulfate plot(T6) was the lowest. The fresh yields of ammonium sulfate plots(T3, T4, T5) and potassium sulfate plot(T6) were higher than that of the urea plot(T2) as $173\%,\;173\%,\;144\%\;and\;90\%$. respectively. The dry matter and total digestible nutrient(TBN) yields were similar tendency like the results of the fresh matter yields. The crude protein(CP), neutral detergent fiber(NDF) and acid detergent fiber(ADF) contents of ammonium sulfate plots(T3, T4, T5) were higher than those of urea plot(T2), but those of potassium sulfate plot(T6) were the lowest. On the other hand, TDN content in potassium sulfate plot(T6) was the highest. It was desirable to use ammonium sulfate$(20\~30kg\;N/10a)$ and potassium sulfate fertilizer$(15kg\;K_2O/10a)$ than those of urea and potassium chloride fertilizer on reclaimed tidal land in view of forage production and its feed value.

A Study on the Plant Planning in Landscape Space Considering the Characteristics of the Gender Determination of Pine Tree (소나무 성 결정 요인의 특성을 고려한 조경공간 식재계획)

  • Lee, Chang-Hun
    • Journal of the Korean Institute of Traditional Landscape Architecture
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    • v.38 no.1
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    • pp.45-52
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    • 2020
  • This study analyzed the components contained in the pine needles of first and second-year-olds to analyze the factors that the in vivo content of inorganic elements affects the sex determination of pine trees. In response, the plan for pine tree plant and maintenance was intended to be presented in consideration of the reproductive environment and physiological characteristics. The results are as follows. First, last year, when there were many encyclopedias, the analyzed N(%) content was found to be high. The nitrogen content of the previous year's soil was found to affect the production of the spheres the following year. This is believed to be possible to reduce the rate of Pine pollen produced in the new plant in the following year through a dispute over quality consumption in the spring of the previous year. Second, the weapons elements involved in the Pine cones and the generation of the Pine pollen in the new plant appeared to be P(%), K(%), Ca(%), and Fe(%). However, the nutrients from the previous year's leaves of Ca(%) and Fe(%) were found to have a low influence on the sex determination of first-year pine trees. Because Ca(%) and Fe(%) are not able to move nutrients accumulated in aging organs due to the nature of the components, feeding nutrients in the fall when the growth of the previous year's branches is reduced is expected to affect oral generation. Third, pine trees are extremely positive and Pine pollen is related to the time of the northeast wind. Therefore, it is believed that it would be good to be located in the northern direction, where the sunlight is good, in an outdoor space. In addition, it is important to use vaginal consumer products in spring and carry out a quarrel involving Mg and Fe during fall to reduce the effect of the Pine pollen, which is an outdoor plant. This is an important part of maintaining and managing pine trees in outdoor spaces as well as the sex determination of pine trees. This study suggested that plant planning, which derives the correlation between pine inorganic element content on sexual determination and takes into account the physiological characteristics of pine trees, is an important issue in the creation of outdoor space. Follow-up research on other factors affecting pine tree sex determination is expected.

Gibberellin Application Method and Concentration Affect to Growth, Runner, and Daughter Plant Production in 'Maehyang' Strawberry during Nursery Period (육묘기 '매향' 딸기의 생육, 런너 및 자묘 생산에 미치는 지베렐린 처리방법 및 농도의 영향)

  • Kang, Jae Hyeon;Kim, Hyeon Min;Kim, Hye Min;Jeong, Hyeon Woo;Lee, Hye Ri;Hwang, Hee Sung;Jeong, Byoung Ryong;Kang, Nam Jun;Hwang, Seung Jae
    • Journal of Bio-Environment Control
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    • v.27 no.4
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    • pp.407-414
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    • 2018
  • This study was aimed to evaluate the effect of application method and concentration of gibberellin $A_3$ ($GA_3$) on the growth, runner production, and seedling quality of strawberry plants (Fragaria ${\times}$ ananassa Duch. cv. Maehyang) during nursery period. The mother plants of strawberry were transplanted in pot ($64{\times}27{\times}18cm$) filled with commercial growing medium on March 20, 2018. $GA_3$ concentration was applied as 0, 50, 100 or $200mg{\cdot}L^{-1}$ with spray or drench to 45 mL per plant at 4 weeks after transplanting, respectively. Nutrient solution was supplied with the EC $1.5dS{\cdot}m^{-1}$ after the transplanting and supplied 350 mL per pot twice a day (15 min per one time) after rooting. The growth characteristics of mother plants of strawberry were measured at 7 weeks after treatment, and growth characteristics of daughter plants of strawberry were measured at 10 weeks after treatment. Runner length and diameter of mother plant was the longest or thickest in the spray with $200mg{\cdot}L^{-1}$ than the other treatments, respectively. Soil-plant analysis development (SPAD) value of mother plant was the lowest in spray with $200mg{\cdot}L^{-1}$. However, leaf length, leaf width, and crown diameter showed no significant differences in all treatment among application method and concentration of $GA_3$. As the concentration of $GA_3$ increased, physiological disorder like stretchiness of crown occurred more. The physiological disorder was the most occurred in spray treatment with $200mg{\cdot}L^{-1}$, but drench treatment occurred less than spray treatment. The number of runners and daughter plants increased with increasing concentration of $GA_3$ regardless of application methods. In the growth characteristics of the daughter plants, leaf length and leaf width of first daughter plant, plant height, crown diameter, leaf area and SPAD value of second daughter plant, and plant height of third daughter plant were the significantly greatest in drench with $100mg{\cdot}L^{-1}$ treatment. This results indicate that growth and runner production of mother plants and growth of daughter plants of strawberry were the best achieved by drench application in the $100mg{\cdot}L^{-1}$ $GA_3$.