• Korean Journal of Soil Science and Fertilizer
• /
• v.3 no.1
• /
• pp.55-59
• /
• 1970

In an effort to determine the bio-synthesis in the soybean as investigate to the variance of each substance: Glutamic acid, Aspartic acid and its amides during the growth of younger soybean plants. 1. The variance-curve of Gultamic acid and Aspartic acid as the acidic amino acids in the cotyledons was appeared the peak the first half period at Glutamic acid and the latter half at Aspartic acid in the growth of soybeans, and was received the symmetrical impression centering around the stage of adult leaf-development. But, in the embryonic organ, it appears the peak at both part, in the developmental stage of adult leaf and also appears near phenomena of increase and decrease in the variation-curve of metabolites. 2. It's amides-Gultamine and Asparagine-appears the peak at the developmental stage of adult leaf in the both cotyledons and embryonic organ, and rapid increase in the cotyledons were very impressed compare with the decrease at fallen stage of cotyledons in the embryonic organs. 3. In the relation of variance at Glutamic acid and Aspartic acid, both substance were discovered the fact of translocation from cotyledon to embryonic organ, and Glutamic acid could supposed that bear the charges of outrider substance in other amino acid as the Glutamic acid-self and major basic function for receiving the ammonia as the nitrogen contain constituent of plant. In the case of Glutamine, formation-mechanism of ammonia which develops due to its hydrolysis in the latter period of soybean growth, suggested that was forfeit its function till instance of fallen cotyledons. 4. In the relation the Aspartie acid and Asparagine, Aspartic acid which begins to decrease from seed-state was supposed that bear sufficiently the charge of outrider substance in the formation of Asparagine other than translocated to embryonic organ from cotyledon. And, formation-theory of Aspartic acid which suppose as formational substance from Kreb's cycle were recognized from latter period of soybean growth, and then, rapid accumulation of Asparagine's amounts were supposed that adapt to two theory: Theory which consider to transformation as Asparagine state for pressing to less than noxious weight the concentration of ammonia developing from the cells, and was formate and accumulate as ammonia or carbohydrates containing excess in the cotyledons.

• Korean Journal of Soil Science and Fertilizer
• /
• v.27 no.1
• /
• pp.15-20
• /
• 1994

Lysimeter experiments were conducted to elucidate the behavior of $NO_3-N$ derived from urea applied at different rates and accompanying cations in soils and to further provide fundamental information of rational nitrogen-fertilizer management. Urea was applied at rates of 0, 7, 14, 21, 28 and 35kg N/10a to sandy loam pakced into PVC cylindrical lysimeter(vol. : $0.187m^2$, area $0.43m^2$). Leachates from the lysimeter with or without grass grown were collected periodically and analyzed for $NO_3$ and cations. Grass growth and yield responses to N fertilization were also examined. Dry matter yield and nitrogen uptake increased with the urea application rate. The amount of leachate from the lysimeter was negatively correlated with urea application ratesl($r=-0.95^{**}$). The nitrate leaching loss with grass grown was 230 g N/10a at the maximum rate of 35kg N/10a, but the highest leaching loss was observed as 1,607 g N/10a from the bare plot. Increase in urea application rates decreased significantly leaching losses of Ca, Mg, K and Na(>0.01). The highest leaching loss from the bare plot was observed for Ca but only 6.5% of exchangeable form and 14.0% for K from the grass plot respectively. Equivalent ratio of cations to nitrate leached were 3.2 % for the bare plot and the ratio for the grass plot increased with the urea application rate, ranging from 18.6 to 32.7%.

• Journal of The Korean Society of Grassland and Forage Science
• /
• v.30 no.1
• /
• pp.15-24
• /
• 2010

This study was conducted to investigate the effects of swine manure (SM) application with additional nitrogen (N) fertilizer on productivity of corn and environmental pollution in com cultivation soil. The experiment was conformed in lysimeter which was constructed with 30 cm diameter, and 100 cm height. Swine manures used in this study were the compost of swine manure fermented with sawdust (SMFWS) and soft rice hulls treated with high temperature and high pressure (SRH), and swine slurry (SS). The application rates of the additional N, as urea, with swine manure were 50 and 100 kgN/ha on each plot. This study was arranged in completely randomized design with three replication. DM yields in SM treatments with mineral N were increased significantly compared to those in SM treatment without mineral N (zero-mineral N) (P<0.05) and increased as the rate of mineral N application increased (P<0.05). DM yields in SMFWS and SS treatments with mineral 100 kgN/ha showed trends similar to those of chemical fertilizer (control) but higher than those of 50 kg N/ha. DM yields tended to be higher in SMFWS and SS treatments than in SRH treatment. Total N contents in SMFWS, SRH and SS treatments with mineral N were increased, compared with SM treatment without mineral N. N contents in SM treatments with mineral N were lower than those of chemical fertilizer treatment, but N content of chemical fertilizer treatment showed trends similar to that of SS treatments with mineral 100 kgN/ha. $NO_3$-N concentration in SM treatments with mineral N were increased significantly, compared to those in SM treatment without mineral N and in chemical fertilizer (P<0.05). $NH_4$-N concentrations in SMFWS and SS treatments with mineral 100 kgN/ha showed trends similar to those of chemical fertilizer, but higher than those of 50 kg N/ha. $PO_4$-P concentration in SM treatments with mineral N were increased significantly, compared to those in SM treatment without mineral N (P<0.05). $PO_4$-P concentration in chemical fertilizer treatment showed trends similar to that of SS treatments with mineral 100 kgN/ha. The concentrations of $NO_3$-N $NH_4$-N and $PO_4$-P increased as the rate of mineral N application increased (P<0.05). The concentrations of $NO_3$-N $NH_4$-N and $PO_4$-P were highly elevated in the concentrated rainy season in the early stage among experimental period. The maximum $NO_3$-N $NH_4$-N and $PO_4$-P concentrations in the leaching water were 3.46 mg/L, 1.11 mg/L and 0.14 mg/L, respectively.

• Korean Journal of Soil Science and Fertilizer
• /
• v.21 no.3
• /
• pp.254-263
• /
• 1988

Contents and distribution of amino acids in the humic acid and fulvic acid fractions of different types ($R_p$, B, A, P) were investigated. Extracted humic and fulvic acids were purified and analyzed. The results are summarized as the following: (1) Composition of Humus The total humus ($H_T$), amount of humic acid (a), amount of fulvic acid (b), and ${\Delta}logK$ all decrease in the order of $R_p$ > B > A > P type. The same trend was observed in the total nitrogen and carbon. (2) Contents and composition of amino acids in humic acids. 1) The total amounts of amino acids in the humic acid fraction of different types were in the following order for soils under coniferous forest trees: $R_p$ > B > A > P type, but for soils under deciduous forest trees the order was P > A > $R_p$ > B type. There were positive correlationships between total amino acids and total carbon and ${\Delta}logK$ for humic acids from soils under coniferous forest trees, but a negative correlationship was existed. between total amino acids and C/N ratios. No significant correlation was found for samples taken from soils under deciduous forest trees. 2) The ratios of one group of amino acids to the others were compared. The ratios of acidic amino acids were in the order of P > $R_p$ > B > A type. those of neutral amino acids followed the order of $R_p$ > B > A > P type and those of the basic amino acids were in the order of B > A >$R_p$ > P type for soils under coniferous forest trees. Contents of total amino acids were in the order of the neutral > the acidic > the basic amino acids. For the soils under deciduous forest trees the order of the ratio was different. Acidic amino acids followed the order of A > P > B > $R_p$ type, neutral ones followed the order of P > $R_p$ > A > B type, and the basic amino acids did the order of $$P{\geq_-}$$ A > B $$\geq_-$$ $-R_p$ type where the difference was very small. 3) In general aspartic aicd, glycine and glutamic acid were the major components in all samples. Histidine, tyrosine and methionine belonged to the group contained in a small amount. (3) Contents and composition of amino acids in fulvic acids. 1) The total amounts of amino acids of different types of fulvic acids were in the order of $R_p$ > B > P > A type regardless of origin of samples. There were positive correlationships observed between the toal amino acids and total carbon and ${\Delta}logK$ for soils under coniferous forest trees. For soils under deciduous forest trees, positive correlationships were observed among total amino aicds, total nitrogen, total humus ($H_T$), total humic aicd (a), and ${\Delta}logK$, but a negative correlationship existed between total amino acids and C/N ratio. 2) Thr ratio among acidic amino acids, neutral amino acids and basic amino acids of different types were $R_p$ > B > P > A type. In this respect there was no difference between the two soils. 3) In general glycine, aspartic acid, and alanine were the major constituents in all samples of different types, while tyrosine and methionine were contained in a small amount. Virtually no amount of arginine was measured.

• Korean Journal of Environmental Agriculture
• /
• v.21 no.4
• /
• pp.248-254
• /
• 2002

This study was carried out to evaluate the proper application amount of anaerobic digestion waste water and the environmental influence on rice. The waste water collected after methane fermentation process of pig manure was used as a liquid manure. Liquid manure 100%+chemical fertilizer 30%(LM 100%+CF 30) treatment was the most favorable at all growth stages of rice. The LM 100%+CF 30% treatment was applied to 100% amount of liquid manure which was correspond to the same amount of nitrogen for the standard application amount on rice, with adding 30% amount of chemical fertilizer(urea) at tillering stage. The yields of rice in the treatments of 100%(LM 100%) and 150% amount(LM 150%) of liquid manure were similar or a little higher than NPK treatment but LM 100%+CF 30% treatment was less than the NPK treatment due to the increase of straw weight and plant lodging. In periodic changes of the $NH_4-N$ and $NO_3-N$ contents, the LM 70%+CF 30% treatment in paddy soil was the highest in all treatments. The NPK and the LM 100% treatments in irrigation water quality were higher than other treatments. In infiltration water quality, $NH_4-N$ content was leached out much in the LM 150% treatment and $NO_3-N$ content was in the LM 100%+CF 30% treatment. The proper application amount of anaerobic digestion waste water as a liquid manure must be to analyse the nitrogen content of the waste water and to apply the same amount of nitrogen for the standard application amount on rice.

• Korean journal of food and cookery science
• /
• v.11 no.4
• /
• pp.356-361
• /
• 1995

This study was conducted to know the quality of marketed green teas which were devided into high, middle and low grade according to the price. we examined the content of water, ash, total nitrogen, tannin, caffeine, chlorophyll and color value and did sensory evaluation. The results were as follows: 1) The content of water was 2.17-3.67％ and content of ash was 4.50-5.17％, there were no significant difference in each grade. 2) The content of total nitrogen was 4.38％ in high grade, 4.60％ in middle grade and 4.68％ in low grade. 3) The content of tannin was 11.09％ in high grade, 14.22％ in middle grade and 14.44％ in low grade, middle and low grade were significantly higher than high grade. 4) The nitrogen rate to tannin (N/T) was 39.5％ in high grade, 32.6％ in middle grade and 32.70％ in low grade, N/T rate of high grade was significantly highest than others. 5) The content of caffeine is 1.78％ in high grade, 1.32％ in middle grade and 0.92％ in low grade, high and middle grade were significantly higher than low grade. 6) The content of total chlorophyll is 297.37 mg％ in high grade, 192.89 mg％ in middle grade and 204.79 mg％ in low grade, chlorophyll a was 69.23 mg％ in high grade, 51.99 mg％ in middle grade and 63.42 mg％ in low grade, and chlorophyll b was 228.47 mg％ in high grade, 131.21 mg％ in middle grade and 141.63 mg％ in low grade. 7) Yellow value of high and middle grade were significantly higher than low grade, but blue and red value were no significant diffrence in groups. 8) In sensory evaluation, appearance, taste, odor of high grade were better than others and total score was 15.25 in high grade, 12.97 in middle grade and 9.80 in low grade. 9) Price had a positive correlation with caffeine, appearance and taste, but a negative correlation with tannine. NT rate had a negative correlation with tannine, but a positive correlation with caffeine.

• Microbiology and Biotechnology Letters
• /
• v.34 no.1
• /
• pp.7-14
• /
• 2006

Feather, generated in large quantities as a byproduct of commercial poultry processing, is almost pure keratin, which is not easily degradable by common professes. Four strains, SMMJ-2, FL-3, NO-4 and RM-12 were isolated from soil for production of extracellular keratinolytic protease. They were identified as Bacillus sp. based on their morphological and physiological characteristics. They shown high protease activity on 5.0% skim milk agar medium and produced a substrate like mucoid on keratin agar medium. Bacillus sp. SMMJ-2 had a faster production time for producing keratinolytic protease than other strains. This strain did not completely degrade whole chicken feather for five days in basal medium but completely degraded whole chicken feather when supplied with nitrogen source for 40hours in keratinolytic producing medium ($0.7%\;K_{2}HPO_{4},\;0.2%\;KH_{2}PO_{4},\;0.1%$ fructose, 1.2% whole chicken feather, $0.01%\;Na_{2}CO_3$, pH 7.0). When supplied with chicken feather as nitrogen source, keratinolytic protease activity was 89 units/ml/min. When soybean meal was used as nitrogen source, the keratinolytic protease production reached a maximum of 106 units/ml/min after 48 hours under $30^{\circ}C$, 180 agitation. To isolate the keratinolytic protease, the culture filtrate was precipitated with $(NH_4)_{2}SO_4$ and acetone. The recovery rate of keratinolytic protease was about 96% after treatment with 50% acetone. The enzyme was stable in the range of $30{\sim}50^{\circ}C$ and pH $6.0{\sim}12.0$.

• The Korean Journal of Mycology
• /
• v.2 no.1
• /
• pp.1-6
• /
• 1974

1. The mycelial growth and sporulation of Mycogone perniciosa was compared on modified Czapek's media deficient in carbon source, nitrogen source, K,Mg, P or the heavy metal elements. The mycelial growth was significantly reduced in solution cultures lacking Mg, K or P and only a trace of growth occurred in solutions lacking carbon source or nitrogen source. Most sparse sporulation and smaller chlamydospores than on any of deficient agar media occurred on agar media dificient in carbon source or nitrogen source. 2. In both potato dextrose agar and malt extract solution, growth of the fungus was optimum at $25^{\circ}C$, and undetectable at $10^{\circ}C$ and $35^{\circ}C$. 3. Optimum pH for growth of this fungus was 7.0. 4. This fungus was killed in soil when exposed to $50^{\circ}C$ or higher for 20 minutes or more.

• Journal of Animal Environmental Science
• /
• v.18 no.3
• /
• pp.235-246
• /
• 2012

Reusing livestock manure have various advantages in securing soil organic resources. In this study, the N-loading and recycling capacity assessment of animal manure was analyzed by comparing between the cultivated areas of crops and the amount of manure units that are generated from livestock manure. From this assessment, the possibility of recycling resources of livestock manure was evaluated. The amount generated of livestock manure in Gyeonggi-do were evaluated by applying the manure units to the number of livestock manure. The analysis of manure unit per ha shows that the N loading by MU is quite different by region. When it comes to nitrogen loading, the MU per ha of cultivated land in excess of the N-amount was the highest in the Gyeonggi-do province with 2.70 MU/ha, which is higher than the appropriate level. The Chungcheongnam-do province came next with 2.31 MU. So the recycling capacity assessment was carried out mainly based on areas of forage crops, rice that can be provided by recycling livestock manure. The recycling capacity ware highest in Jeollanam-do and Jeollabuk-do. In order to properly apply the livestock manure into organic resources, the seasonal situation that effects the nitrogen demands of crops along with the regional effects crop cultivation should be seriously analyzed. In addition, a system that can effectively produce and manage fertilizer should be established.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.55 no.4
• /
• pp.333-338
• /
• 2010

The use of green manure crop for sustainable agriculture can reduce the use of chemical fertilizer and herbicides, and the cultivation area of the green manure crop has gradually increased. However, there has been little information about appropriate use of spring-sown green manure crop in the central regions of Korea. This study was conducted to investigate the effect of different legume crops on application of sown green manure in spring. Each of the green manure crops including alfalfa, chinese milk vetch, crimson clover, crotalaria, hairy vetch, lupin, red clover and white clover was grown in upland soil of silt loam. The dry weight and C/N ratio of all crops increased throughout the growing period, while C/N ratio of all crops during growing period was lower than 25. The highest value of dry weight among the green manure crops was observed in crimson clover, followed by red clover, lupin, chinese milk vetch and alfalfa. Also, the highest value of contents of nitrogen, phosphous and potassium of green manure crops were observed in hairy vetch, alfalfa and crimson clover, respectively. And the values were 41.3, 4.3 and 35.9 g $kg^{-1}$, respectively. In terms of nitrogen yield, crimson clover that showed 71 kg N $ha^{-1}$ was the highest yield among the green manure crops, followed by chinese milk vetch of 51 kg $ha^{-1}$, red clover of 46 kg $ha^{-1}$, and hairy vetch of 41 kg $ha^{-1}$. These results suggest that crimson clover, chinese milk vetch, red clover, and hairy vetch could be a suitable green manure crop for spring sowing.