• 한국지하수토양환경학회:학술대회논문집
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• 한국지하수토양환경학회 2001년도 제3회 부산.경남지부 심포지엄
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• pp.14-28
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• 2001

As basic study for purpose bioremedation in oil-contaminated environment, Primarily, we isolated biosurfactant producer- strains utilized of oil-agar plate, and measured surface tension and emulsifying activity. We investigated in oil-contaminated soil and sea water. In this laboratory, Pseudomonas sp. EL-012S strain isolated from oil-contaminated soil was able to product novel biosurfactant under the optimal culture condition. Its condition was n-hexadecane 2.0%, NH$_4$NO$_3$0.4%, Na$_2$HPO$_4$0.6%, KH$_2$PO$_4$0.4%, MgSO$_4$.7$H_2O$ 0.02%, CaCl$_2$.2$H_2O$ 0.001%, FeSO.7$H_2O$ 0.001%, initial pH 7.0 and aeration at 3$0^{\circ}C$, respectively. This biosurfactant was produced in both late-exponential and early-stationary phase. The biosurfactant from Pseudomonas sp. EL-012S was composed of carbohydrate, lipid and protein. The purified-biosurfactant was examined two (biosurfactant type I, II) with the silica gel G60 column chromatography and the purified biosurfactant confirmed thin layer chromatography, high performed liquid chromatography and gas chromatography. The biosurfactant type I involved in carbohydrate-lipid-protein characteristics lowered surface tension of water to 27dyne/cm and interfacial tension 4.5dyne/cm aginst to n-hexadecane and the biosurfactant type B involved in carbohydrate lipid characteristics lowered surface tension of water to 30dyne/cm and interfacial tension 8dyne/cm against to n-hexadecane. Specially type I had the properties such as strong emulsifying activity, emulsion stability, pH-stability, thermo-stability, high cleaning activity and forming ability.

• 한국지하수토양환경학회지:지하수토양환경
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• 제21권1호
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• pp.94-103
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• 2016

Landfarming that supplies aerobic biodegradation condition to indigenous microbes in soils is a biological remediation technology. In this research, volatilization and biodegradation rate by indigenous microbes in the soil contaminated with total petroleum hydrocarbons (TPH) were measured. Soils were contaminated with diesel artificially and divided into two parts. One was sterilized by autoclave to remove indigenous microorganism and the other was used as it was. Various moisture contents and number of tillings were applied to the soil to find out proper condition to minimize volatilization and enhance bioremediation. Volatilization of TPH was inhibited and biodegradation was enhanced by increase on moisture content. Tilling was usually used to supply air for microbes, but tillings did not affect the growth of microbes in our study. Enough moisture content and proper aeration are important to control volatilization in landfarming. Also, TPH degradation was a function of the microbe counts (x₁), numbers of tilling (x₂), and moisture content (x₃) from the application of the response surface methodology. Statistical results showed the order of significance of the independent variables to be microbe counts > numbers of tilling > moisture content.

• 한국지하수토양환경학회지:지하수토양환경
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• 제17권4호
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• pp.44-50
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• 2012

A new type of chemical oxidation technology utilizing micro bubble ozone oxidizer and a pneumatic fracturing equipment was developed to enhance field applicability of a traditional chemical oxidation technology using hydrogen peroxide as an oxidizer for in-situ soil remediation. To find an efficient way to dissolve gaseous ozone into hydrogen peroxide, ozone was injected into water as micro bubble form then dissolved ozone concentration and its duration time were measured compared to those of simple aeration of gaseous ozone. As a result, dissolved ozone concentration in water increased by 31% (1.6 ppm ${\rightarrow}$ 2.1 ppm) and elapsed time for which maximum ozone concentration decreased by half lengthened from 9 min to 33 min. When the developed pneumatic fracturing technology was applied in sandy loam, cracks were developed and grown in soil for 5~30 seconds so that the radius of influence got longer by 71% from 392 cm to 671 cm. The remediation system using the micro bubble ozone oxidizer and the pneumatic fracturing equipment for field application was made and demonstrated its remediation efficiency at petroleum contaminated site. The system showed enhanced remediation capacity than the traditional chemical oxidation technology using hydrogen peroxide with reduced remediation time by about 33%.

• 한국작물학회지
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• 제31권3호
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• pp.260-269
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• 1986

멀칭방법이 토양물리성 개선 및 참깨의 수량증가에 미치는 효과를 구명하기 위하여 풍년깨 품종을 공시하고, 투명비닐, 흑색비닐, 짚을 멀칭 재료로 하여 시험하였으며, 출아율, 토양물리성, 근량, 잡초량, 증발산량 및 수양을 조사하였던 바, 그 결과를 요약하면 다음과 같다. 1. 전생육기간중 토양수분포텐셜의 변화양상은 처리간에 큰 차리가 없었으며, 무멀칭표준구에 비해 비닐멀칭구는 약 5.4%, 짚멀칭구는 약 2.8%의 수분보지효과가 있었다. 2. 최고지온은 표준구에 비해 투명비닐멀칭구에서 약 4$^{\circ}C$ 높았으며, 짚멀칭구에서는 약 7 $^{\circ}C$ 낮았다. 최저지온은 표준구에 비하여 투명비닐멀칭구에서 약 2 $^{\circ}C$높았고, 짚멀칭구에서 약 3$^{\circ}C$ 낮았다. 3. 생육초기에 투명비닐멀칭구의 토양수분포텐셜, 토양온도, 잡초율, 토양경도 및 토분통기능은 무멀칭표준구보다 양호했으며, 따라서 출아율도 높았다. 4. 생육후기에 토양경도는 무멀칭표준구에서 높았으며 짚멀칭구, 흑색비닐멀칭구, 투명비닐멀칭구의 순으로 낮았다. 공극률, 토양통기능 및 유수속도는 투명비닐멀칭구와 짚멀칭구에서 높은 경향이었다. 5. 총근양은 투명비닐멀칭구에서 많았으며, 흑색비닐멀칭구, 짚멀칭구, 무멀칭표준구의 순으로 감소하였다. 근양은 토심 10cm 이내에 높은 밀도로 분포하고 있으며, 토심이 깊어질수록 급격히 감소하였다. 6. 잡초양은 흑색비닐멀칭구에서 가장 적었으며 무멀칭표준구에서 가장 많았다. 7. 토양경도는 토양통기능( r=-.9439**), 투수속도( ${\gamma}$=-.8229*)와 부의 유의상관을 보이며, 토양통기능과 투수속도는 근양과 유의정상관을 보였고, 근량은 수량( r=-.7378*)과 유의적인 상관관계가 있었다. 8. 처리별 증발산양간에는 유의적인 차리가 없었으나, 투명비닐멀칭구가 다른 처리구에 비하여 높은 경향이었다.

• 한국미생물·생명공학회지
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• 제6권4호
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• pp.161-165
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• 1978

자연계에서 유기산을 생성하는 균주 11 군주를 분리하여 그 중에 구연산 생성균 1종을 선정하여, 동정한 결과 Aspergillus niger로 나타났다. 분리된 균주로 500ml 삼각 flask에 Sakaguchi 배지 100ml를 넣고 3$0^{\circ}C$에서 발효한 결과 구연산이 17g/ι가 생성되었다. 또한 Sakagnchi 배지를 개량한 결과 즉 배지적응, peptone첨가, 통기 효과, methanol첨가 및 포도당 첨가 결과 구연산이 35g/ι가 생성되었다

• 한국미생물·생명공학회지
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• 제28권3호
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• pp.156-160
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• 2000

국내 부존자원인 inulin을 이용하여 fructose syrup 또는 oligosacharide를 생산한는 효소를 발굴하기 위하여 전국적으로 토양 등 시료를 채취하여 inulinase 생성 미생물을 분리하였으며 inulinase 활성이 높은 96-1 균주를 동정한 결과 그람 양성균인 Arthrobacter protophormiae/ramosus인 것으로 잠정 동정되었다 Inulinase 생산을 위한 최적 배지는 무기염류 기초배지에 각 1%(w/v)의 inulin tryptone. $NH_4Cl$이 함유된 배지였으며 퇴적 발효조건은 $35^{\circ}C$초기 pH 7.5 통기량 1vvm 교반속도 200rpm이었다.

• 한국응용과학기술학회지
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• 제9권1호
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• pp.31-40
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• 1992

This study is on the biodegradability of the activated sludge, which used to biodegrade Linear Alkylbenzene Sulfonate(LAS), synthetic detergents and sufactants. The activated sludge in waste water treatment plant is used to the test of biodegradation of anionic surfactants and nonionic surfactants, but it have the periodic change of the biological propety to the lapse of the time. For the puropse of controlling and adjusting of the activated sludge in biodegradation test, we collected microorganisms from the sewage plant and the soil, and then, made the activated sludge in semicontinuous aeration chamber. From determined biodegradation data, and the degree of biodegradability to the LAS, we confirmd the standardized synthetic activated sludge which have more stable biodegradability than the sewage activated sludge. In continuous biodegradation test, LAS(dodecene-1) was biodegraded more than 99%, In 7days by the standardized activated sludge.

• Journal of Plant Biology
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• 제9권1_2호
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• pp.25-30
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• 1966

The writer presented in the report 7th of this series(Chong, 1959. '62 a.b. '64, '65) the result of fertlizer application on Sasamorpha purpurascens var. borealis with regards to soil conditions, climate, planting position etc. and this report is on the effectiveness of fertilizers by kinds of the new (1-year old) and old(3-to 4 years old) Phyllostachys retticulata. The ineffectiveness of fertilizers for old bamboos aged more than 8-years old has been reported previously. The study site was chosen in a bamboo groven situated at Hyunnae Ri, Okkye Myun, Myungju Gun, Kangwon Do. Each plot was so designed that only one individual remained at the center of a circular area with a dimeter of meters by eliminating the clums and severing the rhizomes, of other individuals around it. And, for the fertilizer test, 10 individuals(the new and old: 5 to 5) were observed per kind of fertilizer used; thus marking the total number of 160 plots in all. The second part of this study was conducted for the potted individuals which numbered 48, successfully established ones out of 55 the transplanted to pots. The 48 were divided into 8 treatments: whole fertilization (NPK＋compost＋human manure); NPK; N; K; Human manure; compost; and the control. Amoung of fertilizers applied was 200 grams for the natural plots and 100 grams for pot; it continued for 3 years and applied twice a year (spring and summer) following the theory of Dr. Koichi-ro Ueda(1954, '60 a.b.). Measurements were taken in August each year. The resutls were confirming the fact that the bamboos require a great deal of fertilizers. The results show the following points: 1) Fertilizers were more effective in the 3 to 4 year old bamboos. 2) The order of effectiveness of fertilizers by kind was the whole fertilization, NPK, and N treatments for Phyllostachys reticulata. 3) Organic matter in addition to commercial fertilizers showed better result than the soil application of the latter due to better soil aeration.

• 약학회지
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• 제24권2호
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• pp.87-95
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• 1980

Chlorination to polluted water can produce chlorocompounds which may impair human health. It has been discussed that chlorophenols would be one of undesirable substances in drinking water. This study was undertaken to investigate the production mechanism of chlorophenols by chlorination in the disinfection of water and to determine pollution levels of phenols as precursor of chlorophenols and chloropbenols in some sewage, stream water and tap water in the vicinity of Seoul from January to September, 1979. By chlorination with hyperchlorite to phenols in distilled water, o-chlorophenol was predominantly produced at the concentration of less than 10ppm of free chlorine. o-Chlorophenol, 2,6-dichlorophenol and 2,4-dichlorophenol were also produced by chlorination with the concenration from 20 to 100ppm of free chlorine. From the concentration of 100ppm of free chlorine to 200ppm, o-Chlorophenol was vanished and 2,6-dichlorophenol and 2,4-dichlorophenol were determined. Phenols originated from night soil, municipal sewage and stream were determined at 49.15 ppm. 0.095 ppm and 0.003 ppm in average respectively. About 87 and 88 percent of phenols in sewage and night soil were biodegradated by aeration for 10 days and 74 and 51 percent of phenols in sewage and night soil by spontaneous settling for 10 days. From the tap water in Seoul during summer, 1979, chlorophenols were identified; they were average 0.042 ppb of o-chlorophenol, 0.033 ppb of 2, 6-dichlorophenol and 0.003 ppb of 2, 4-dichlorophenol respectively. With the above result and discussion, it is considered that chlorophenols should be controlled from the source as well as chlorination in water purification.

• Horticulture, Environment, and Biotechnology : HEB
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• 제59권6호
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• pp.775-786
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• 2018

To test the hypothesis that humic acid (HA), anaerobically digested pig slurry filtrate (APS), and their combination would differently affect the chemical speciation and extractability of metals (cadmium, copper, and zinc) and their uptake by plants, we conducted a pot experiment using wormwood in two texturally contrasting soils (sandy loam and clay loam) collected from a field near an abandoned Cu mine. Four treatments were laid out: HA at $ 23.5g\;kg^{-1}$ (HA), APS at $330mL\;kg^{-1}$ (APS), HA at $ 23.5g\;kg^{-1}$ and APS at $330mL\;kg^{-1}$ (HA + APS), and a control. Each treatment affected the chemical speciation and mobility of the metals, and thereby resulting in variable patterns of plant biomass yield and metal uptake. The APS supported plant growth by increasing nutrient availability. HA supported or hindered plant growth by impacting the soil's water and nutrient retention capacity and aeration, in a soil texture-dependent manner, while consistently enhancing the immobilization of heavy metals. Temporal increases in whole-plant dry matter yield and metal accumulation suggested that the plants were capable of metal hyperaccumulation. The results were discussed in terms of the mobility of metals and plant growth and corroborated by the $^{15}N$ recovery of soil- and plant-N pools under H and HS treatments. Therefore, for effective phytoremediation of polluted soils, an appropriate combination of plant growth promoters (APS) and chelating agents (HA) should be predetermined at the site where chemical stabilization of pollutants is desired.