• Title/Summary/Keyword: Soil Culture

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Effect of Submergence and Air Exposure of the Shoot on Growth, Nutrient Uptake and Photosynthesis in Monochoria vaginalis Presl. (물달개비 경엽(莖葉)의 침수여부(沈水與否)에 따른 생장(生長), 양분흡수(養分吸收) 및 광합성(光合成) 비교(比較))

  • Soh, C.H.;Yang, K.S.;Kwon, Y.W.
    • Korean Journal of Weed Science
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    • v.16 no.1
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    • pp.14-20
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    • 1996
  • Growth, nutrient uptake and photosynthesis as affected by submersion of shoot in pickerel weed (Monochoria vaginalis Presl.) were determined. The shoots of pickerel weeds in hydroponic culture were subjected to the submerged or emerged condition at 3- or 5-leaf stage for 8 or 10 days. Under submerged condition, growth in plant height was enhanced, but leaf number, leaf area, fresh and dry weight were reduced compared to those under the emerged condition. Similar responses in growth to submergence were obtained with the pickerel weeds rooted in the soil. Under submergence, chlorophyll content increased during the first 2 days, but thereafter remarkably decreased at 3-leaf stage and after the first 4 days at 5-leaf stage. Compared to the emerged condition, uptakes of $NH_4\;^+$-N, $NO_3\;^-$-N, $P_2O_5$ and $K^+$ were reduced, but uptakes of $Ca^{++}$ and $Mg^{++}$ increased under the submerged condition. Photosynthetic rate of shoot under water, measured by $CO_2$electrode, showed the maximum by 210 ${\mu}$moles $HCO_3\;^-$/g F.W. at the 8th day after submergence(DAS) at 3-leaf stage and 320 ${\mu}$moles $HCO_3\;^-$/g F. W. at 6 DAS at 5-leaf stage. These results indicate that pickerel weeds grow much better when the shoot is air-exposed and are less tolerable to submergence at 3 leaf-stage than at 5-leaf stage.

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In vitro Culture and Acclimatization of Regenerated Plants of Liliem cernum $K_{OMAROV}$ (솔나리 기내배양 및 재분화 식물체의 토양순화)

  • Kim, H.K.;Lim, Jung-Dae;Hyun, Tae-Kyung;Lee, Hyeon-Yong;Lee, Jin-Ha;Yu, Chang-Yeon
    • Korean Journal of Medicinal Crop Science
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    • v.9 no.4
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    • pp.310-317
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    • 2001
  • The regenerated-bulblets placed in liquid free media resulted in good formation of roots and bulblets. On 1/4 MS free medium, roots and bulblets were predominantly induced. The 1/4 MS liquid medium supplemented with plant growth regulators was the best suitable condition for elongation of leaves and roots. Somatic embryos were frequently developed from embryogenic callus in liquid media with 2,4-D 1mg/ l . On free liquid media, the viability of callus reduced. As the salt strength of MS media reduces, the viability of callus reduced significantly. However, Leaves were induced from several callus clumps. When leaves, roots and bulb-scale segments were placed on MS media containing NAA 1mg/ l or 2,4-D 1mg/ l and various sucrose concentration, the best result about the differentiation, growth of leaf and the differentiation of leaf was obtained on MS media added 1.5% sucrose and 2,4-D 1mg/ l, 3% sucrose and NAA 1mg/ l, and 1.5% sucrose and NAA 1mg/ l, respectively. Also the better result differentiation, growth of root and differentiation of bulb was obtained on MS media with 6% sucrose and NAA 1mg/ l. Spermidine promoted the growth of leaf and the differentiation of bulb. However, spermine promoted the differentiation of leaf, the differentiation and the growth of root in MS solid media. On the MS liquid media, both spermine and spermidine stimulated organogenesis from bulb-scale segments. Regenerated plantlets were acclimatizated and grown in greenhouse in vermiculite + perlite (1 : 1 by volume) well. The optimal soil condition of rooting for plantlets regenerated was in peat moss.

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Identification of Antagonistic Bacteria, Pseudomonas aurantiaca YC4963 to Colletotri­chum orbiculare Causing Anthracnose of Cucumber and Production of the Antibiotic Phenazine-l-carboxylic acid (Colletotrichum orbiculare에 대한 길항세균 Pseudomonas aurantiaca YC4963의 분리 동정 및 항균물질 Phenazine-1-carboxylic acid의 생산)

  • Chae Hee-Jung;Kim Rumi;Moon Surk-Sik;Ahn Jong-Woong;Chung Young-Ryun
    • Korean Journal of Microbiology
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    • v.40 no.4
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    • pp.342-347
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    • 2004
  • A bacterial strain YC4963 with antifungal activity against Colletotrichum orbiculare, a causal organism of cucumber anthracnose was isolated from the rhizosphere soil of Siegesbeckia pubescens Makino in Korea. Based on physiological and biochemical characteristics and 16S ribosomal DNA sequence analysis, the bac­terial strain was identified as Pseudomonas aurantiaca. The bacteria also inhibited mycelial growth of several plant fungal pathogens such as Botrytis cinerea, Fusarium oxysporum and Rhizoctonia solani on PDA and 0.1 TSA media. The antifungal activity was found from the culture filtrate of this isolate and the active compound was quantitatively bound to XAD adsorption resin. The antibiotic compound was purified and identified as phenazine-l-carboxylic acid on the basis of combined spectral and chemical analyses data. This is the first report on the production of phenazine-l-carboxylic acid by Pseudomonas aurantiaca.

Trend and Further Research of Rice Quality Evaluation (쌀의 품질평가 현황과 금후 연구방향)

  • Son, Jong-Rok;Kim, Jae-Hyun;Lee, Jung-Il;Youn, Young-Hwan;Kim, Jae-Kyu;Hwang, Hung-Goo;Moon, Hun-Pal
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.47
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    • pp.33-54
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    • 2002
  • Rice quality is much dependent on the pre-and post harvest management. There are many parameters which influence rice or cooked rice qualitys such as cultivars, climate, soil, harvest time, drying, milling, storage, safety, nutritive value, taste, marketing, eating, cooking conditions, and each nations' food culture. Thus, vice evaluation might not be carried out by only some parameters. Physicochemical evaluation of rice deals with amy-lose content, gelatinizing property, and its relation with taste. The amylose content of good vice in Korea is defined at 17 to 20%. Other parameters considered are as follows; ratio of protein body-1 per total protein amount in relation to taste, and oleic/linoleic acid ratio in relation to storage safety. The rice higher Mg/K ratio is considered as high quality. The optimum value is over 1.5 to 1.6. It was reported that the contents of oligosaccharide, glutamic acid or its derivatives and its proportionalities have high corelation with the taste of rice. Major aromatic compounds in rice have been known as hexanal, acetone, pentanal, butanal, octanal, and heptanal. Recently, it was found that muco-polysaccharides are solubilized during cooking. Cooked rice surface is coated by the muco-polysaccharide. The muco-polysaccharide aye contributing to the consistency and collecting free amino acids and vitamins. Thus, these parameters might be regarded as important items for quality and taste evaluation of rice. Ingredients of rice related with the taste are not confined to the total rice grain. In the internal kernel, starch is main component but nitrogen and mineral compounds are localized at the external kernel. The ingredients related with taste are contained in 91 to 86% part of the outside kernel. For safety that is considered an important evaluation item of rice quality, each residual tolerance limit for agricultural chemicals must be adopted in our country. During drying, rice quality can decline by the reasons of high drying temperature, overdrying, and rapid drying. These result in cracked grain or decolored kernel. Intrinsic enzymes react partially during the rice storage. Because of these enzymes, starch, lipid, or protein can be slowly degraded, resulting in the decline of appearance quality, occurrence of aging aroma, and increased hardness of cooked rice. Milling conditions concerned with quality are paddy quality, milling method, and milling machines. To produce high quality rice, head rice must contain over three fourths of the normal rice kernels, and broken, damaged, colored, and immature kernels must be eliminated. In addition to milling equipment, color sorter and length grader must be installed for the production of such rice. Head rice was examined using the 45 brand rices circulating in Korea, Japan, America, Australia, and China. It was found that the head rice rate of brand rice in our country was approximately 57.4% and 80-86% in foreign countries. In order to develop a rice quality evaluation system, evaluation of technics must be further developed : more detailed measure of qualities, search for taste-related components, creation and grade classification of quality evaluation factors at each management stage of treatment after harvest, evaluation of rice as food material as well as for rice cooking, and method development for simple evaluation and establishment of equation for palatability. On policy concerns, the following must be conducted : development of price discrimination in conformity to rice cultivar and grade under the basis of quality evaluation method, fixation of head rice branding, and introduction of low temperature circulation.

Study on Cultural Method of Summer Buckwheat Planted in Spring (여름메밀의 춘파재배법 연구)

  • Keun-Yong Park;Rae-Kyung Park;Byeong-Han Choi
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.37 no.2
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    • pp.149-154
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    • 1992
  • Buckwheat has been a popular favorite food crop in Korea for a long time. The objective of the study was to investigate the effects of climatic conditions and cultural methods on grain yields of summer buckwheat variety Sinnong 1 planted during the spring season in Suwon, Korea from 1989 to 1991. Frost minimum temperature of late April was -0.3$^{\circ}C$ in 1990 being very low as compared with 3.7$^{\circ}C$ of the normal year, and affected early growth of the seedlings emerging from the soil surface. In late May of 1990, the frost minimum temperature was 7.3$^{\circ}C$ being low as compared with 8.8$^{\circ}C$ of the normal year, and also induced cold injury to fertilization and grain filling. Total precipitation 374.5mm of mid and late June, 1990 provided serious damage to the grain filling and maturing buckwheat seeds and along with causing seed sprouting before harvest. However, the climates of 1989 and 1991 were very good for the growth and development of spring-sown buckwheats. When summer buckwheat cultivar Sinnong 1 was planted on April 20, 1989, its highest grain yields 268-292kg /10a were harvested from the plots of seeding rate 8kg /10a, drill seeding and polyethylene film mulching, and the mean grain yield of the plots was 238kg /10a in 1989, but 64.3kg in Suwon, and 40.2kg /10a in Muan in 1990. In 1991 maximum grain yield 277kg /10a was produced from the April 15 planted and vinyl-mulched plot, and 255kg /10a from the April 25 planted and non-mulched plot. Herbicide Alachlor-sprayed plots produced lower grain yields than no weed control and manual weeding plots. Mechanized drill-seeding saved 83~84% in planting hours as compared with manual broadcasting 21.6 hours /ha, and produced 9% more in grain yields from the two-season croppings of mechanized drill-seeding culture being 364kg /10a in total yields per year.

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Studies on the Growth and nutritional Behaviors of Seedlings Sand Cultured at Different pH Levels (pH 수준별(水準別) 사경묘목(砂耕苗木)의 생장(生長)과 영양동태(營養動態)에 관(關)한 연구(硏究))

  • Son, Won Ha
    • Journal of Korean Society of Forest Science
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    • v.11 no.1
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    • pp.1-24
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    • 1970
  • This experiment was carried out to elucidate the behavior of nutrition absorption and the acid tolerance, on varying soil pH levels, of Pinus thunbergii, Pinus densiflora, Pinus rigida, PInus koraiensis, Larix leptolepis and Abies holophylla which are 6 month old seedlings through examinations of the absorption behavior, the acid and alkali tolerance of N,P,K, Ca and Fe in the sand culture media with artificially controlled pH. 1. The increase rate of dry matter conditioned by the behavior of nutrition absorption in a strong acidic condition (pH3) was found in the following decreasing order of Pinus rigida, Pinus thunbergii. This order, therefore, can also be said to apply to the case of acid tolerance. 2. The increase rate of dry matter in a strong alkaline condition (pH9) was found in the following decreasing order of Pinus thunbergii, Pinus rigida, Pinus densiflora, Pinus koraiensis, Abies hoplohylla, Larix leptolepis. This order, therefore can be said to apply to the case of alkali tolerance. 3. Considering the absorption behavior of K and Ca, it is possible to decide the tolerance of acid from the absorption behavior and it can be said that if the absorption quantity is K>Ca the acid tolerance is strong and vice versa. 4. Considering the ratio of K:Fe and Ca:Fe in this experiment, a strong acid tolerance shows a higher ratio of K/Fe and vice versa. And also a strong acid tolerance shows a lower ratio of Ca/Fe and vice versa. It is therefore possible to decide the acid tolerance from the absorption behavior of K, Ca and Fe. 5. Of the tree species subjected to this experiment, Pinus thunbergii was the strongest in both acid and alkali tolerance. 6. In spite of that in all species the dry weight in the root is smailer than that in the top, the quantity of Fe contained in the root was larger than that in the top.

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Field Survey of Greenhouse for Strawberry Culture -Case Study Based on Western Gyeongnam Area- (딸기재배 온실의 현장조사 분석 -서부경남 지역을 중심으로-)

  • Jeong, Young Kyun;Lee, Jong Goo;Yun, Sung Wook;Kim, Hyeon Tae;Yoon, Yong Cheol
    • Journal of Bio-Environment Control
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    • v.27 no.3
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    • pp.253-259
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    • 2018
  • This study set out to select a system to realize an optimal environment for strawberry cultivation greenhouses based on data about the growth and development of strawberry and its environment and to provide basic data for the research of its improved productivity. For these purposes, the investigator conducted a field survey with greenhouses for strawberry cultivation in western Gyeongnam. The findings show that farmers in their fifties and sixties accounted for the biggest part in the age groups of strawberry farmers. While those who were under 50 were accounted for approximately 67.5%, those who were 60 or older accounted for 32.5%. As for cultivation experiences, the majority of the farmers had ten years of cultivation experiences or less with some having 30 years of cultivation experiences or more. All the farmers built an arch type single span greenhouse. Those who used nutrient solutions were about 75.0%, being more than those who used soil. All of the farmers that used a nutrient solution adopted an elevated hydroponic system. The single span greenhouses were in the range of 7.5~8.5m, 1.3~1.8m and 2.5~3.5m for width, eaves, and ridge height, respectively, regardless of survey areas. The rafters interval was about 0.7~0.8m. In elevated hydroponic cultivation, the width, height, and interval of the beds were about 0.25m, 1.2m and 1.0m, respectively. As for the strawberry varieties, the domestic ones accounted for approximately 97.5% with Seolhyang being the most favorite one at about 65.0%. As for the internal environment factors of greenhouses, 38 farmers measured only temperature and relatively humidity. As for hydroponics, the farmers used a hydroponics control system. Except for the farmers that introduced a smart farm system for temperature and humidity control, approximately 85.0% controlled temperature and humidity only with a control panel for side windows and ventilation fans. As for heating and heat insulation, all of the farmers were using water curtains with many farmers using an oil or electric boiler, radiating lamp or non-woven fabric, as well, when necessary.

THE ECOLOGY, PHYTOGEOGRAPHY AND ETHNOBOTANY OF GINSENG

  • Hu Shiu Ying
    • Proceedings of the Ginseng society Conference
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    • 1978.09a
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    • pp.149-157
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    • 1978
  • Ginseng is the English common name for the species in the genus Panax. This article gives a broad botanical review including the morphological characteristics, ecological amplitude, and the ethnobotanical aspect of the genus Panax. The species of Panax are adapted for life in rich loose soil of partially shaded forest floor with the deciduous trees such as linden, oak, maple, ash, alder, birch, beech, hickory, etc. forming the canopy. Like their associated trees, all ginsengs are deciduous. They require annual climatic changes, plenty of water in summer, and a period of dormancy in winter. The plant body of ginseng consists of an underground rhizome and an aerial shoot. The rhizome has a terminal bud, prominent leafscars and a fleshy root in some species. It is perennial. The aerial shoot is herbaceous and annual. It consists of a single slender stem with a whorl of digitately compound leaves and a terminal umbel bearing fleshy red fruits after flowering. The yearly cycle of death and renascence of the aerial shoot is a natural phenomenon in ginseng. The species of Panax occur in eastern North America and eastern Asia, including the eastern portion of the Himalayan region. Such a bicentric generic distributional pattern indicates a close floristic relationship of the eastern sides of two great continental masses in the northern hemisphere. It is well documented that genera with this type of disjunct distribution are of great antiquity. Many of them have fossil remains in Tertiary deposits. In this respect, the species of Panax may be regarded as living fossils. The distribution of the species, and the center of morphological diversification are explained with maps and other illustrations. Chemical constituents confirm the conclusion derived from morphological characters that eastern Asia is the center of species concentration of Panax. In eastern North America two species occur between longitude $70^{\circ}-97^{\circ}$ Wand latitude $34^{\circ}-47^{\circ}$ N. In eastern Asia the range of the genus extends from longitude $85^{\circ}$ E in Nepal to $140^{\circ}$ E in Japan, and from latitude $22^{\circ}$ N in the hills of Tonkin of North Vietnam to $48^{\circ}$ N in eastern Siberia. The species in eastern North America all have fleshy roots, and many of the species in eastern Asia have creeping stolons with enlarged nodes or stout horizontal rhizomes as storage organs in place of fleshy roots. People living in close harmony with nature in the homeland of various species of Panax have used the stout rhizomes or the fleshy roots of different wild forms of ginseng for medicine since time immemorial. Those who live in the center morphological diversity are specific both in the application of names for the identification of species in their communication and in the use of different roots as remedies to relieve pain, to cure diseases, or to correct physiological disorders. Now, natural resources of wild plants with medicinal virtue are extremely limited. In order to meet the market demand, three species have been intensively cultivated in limited areas. These species are American ginseng (P. quinquefolius) in northeastern United States, ginseng (P. ginseng) in northeastern Asia, particularly in Korea, and Sanchi (P. wangianus) in southwestern China, especially in Yunnan. At present hybridization and selection for better quality, higher yield, and more effective chemical contents have not received due attention in ginseng culture. Proper steps in this direction should be taken immediately, so that our generation may create a richer legacy to hand down to the future. Meanwhile, all wild plants of all species in all lands should be declared as endangered taxa, and they should be protected from further uprooting so that a. fuller gene pool may be conserved for the. genus Panax.

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Isolation and Characteristics of a Phenol-degrading Bacterium, Rhodococcus pyridinovorans P21 (페놀분해세균 Rhodococcus pyridinovorans P21의 분리 및 페놀분해 특성)

  • Cho, Kwang-Sik;Lee, Sang-Mee;Shin, Myung-Jae;Park, Soo-Yun;Lee, Ye-Ram;Jang, Eun-Young;Son, Hong-Joo
    • Journal of Life Science
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    • v.24 no.9
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    • pp.988-994
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    • 2014
  • The effluents of chemical and petroleum industries often contain non-biodegradable aromatic compounds, with phenol being one of the major organic pollutants present among a wide variety of highly toxic organic chemicals. Phenol is toxic upon ingestion, contact, or inhalation, and it is lethal to fish even at concentrations as low as 0.005 ppm. Phenol biodegradation has been studied in detail using bacterial strains. However, these microorganisms suffer from substrate inhibition at high concentrations of phenol, whereby growth is inhibited. A phenol-degrading bacterium, P21, was isolated from oil-contaminated soil. The phenotypic characteristics and a phylogenetic analysis indicated the close relationship of strain P21 to Rhodococcus pyridinovorans. Phenol biodegradation by strain P21 was studied under shaking condition. The optimal conditions for phenol biodegradation by strain P21 were 0.09% $KNO_3$, 0.1% $K_2HPO_4$, 0.3% $NaH_2PO_4$, 0.015% $MgSO_4{\cdot}7H_2O$, 0.001% $FeSO_4{\cdot}7H_2O$, initial pH 9, and $20-30^{\circ}C$, respectively. When 1,000 ppm of phenol was added to the optimal medium, the strain P21 completely degraded it within two days. Rhodococcus pyridinovorans P21 could grow in up to 1,500 ppm of phenol as the sole carbon source in a batch culture, but it could not grow in a medium containing above 2,000 ppm. Moreover, strain P21 could utilize toxic compounds, such as toluene, xylene, and hexane, as a sole carbon source. However, no growth was detected on chloroform.

Effects of Different EC in Nutrient Solution on Growth and Quality of Red Mustard and Pak-Choi in Plant Factory (식물공장내 양액의 EC가 적겨자와 청경채의 생육 및 품질에 미치는 영향)

  • Lee, Sang Gyu;Choi, Chang Sun;Lee, Jun Gu;Jang, Yoon Ah;Nam, Chun Woo;Yeo, Kyung-Hwan;Lee, Hee Ju;Um, Young Chul
    • Journal of Bio-Environment Control
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    • v.21 no.4
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    • pp.322-326
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    • 2012
  • Recently, researches related to plant factory system has been activated and production of Ssam-vegetables using artificial lighting has been increasing. In South Korea, Ssam-vegetables are very popular and the consumption is increasing every year. Because leaf vegetables cultivated under hydroponic systems are more preferable rather than those cultivated by soil culture in Korea, the plant factory system would be more effective in production of Ssam-vegetables. Therefore, this study was carried out in order to analyze the yield and vitamin C contents in red mustard (Brassica juncea L.) and pak-choi (Brassica campestris var. chinensis), which are used a lot for the Ssam-vegetables in South Korea, as influenced by different concentrations of the nutrient solution in a plant factory system. As a results, there was no significant differences in the plant height among the treatment of EC in the nutrient solution, but for red mustard plants, the number of leaves tended to decrease in the treatment with higher EC. Leaf area of pak-choi plants was significantly increased in the higher EC, while the fresh weight had a tendency to increase along with increasing EC in the nutrient solution for both crops. The photosynthetic rates did not show a distinct tendency by EC levels for red mustard plants, but for pak-choi plants, it tended to be higher at the high EC. The contents of ascorbic acid in leaves were higher with decreasing EC concentration in the nutrient solution for red mustard plants, while the content was the highest at EC $2.0dS{\cdot}m^{-1}$ for pak-choi plants. In summary, considering the marketable yields and vitamin C at different nutrient concentrations in a plant factory, the optimal concentration for red mustard and pak-choi plants was thought to be EC $2.0{\sim}2.5dS{\cdot}m^{-1}$.