• 한국균학회소식:학술대회논문집
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• 2015.05a
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• pp.29-29
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• 2015

Clubroot disease is caused by the soil-born obligate plant pathogen Plasmodiophora brassicae. This pathogen can infect all cruciferous vegetables and oil crops, including Brassica rapa, B. oleracea, B. napus, and other Brassica species. Clubroot disease is now considered to be a major problem in Chinese cabbage production in China, Korea, and Japan. We collected several hundreds of P. brassicae infected galls from Korea, and isolated the single spore from the collection. For establishment of novel isolation, and mass-propagation methods for singe spore isolates of P. brassicae pathogen, we developed new filtration method using both cellulose nitrate filter and syringe filter. Accurate detection of P. brassicae pathogen in the field was done by using real-time PCR in the potential infested soil. When we tested the different pathogenicity on commercial Chinese cabbage varieties, P. brassicae from collected galls showed various morphological patterns about clubroot symptom on roots. To date, 8 CR loci have been identified in the B. rapa genome using the quantitative trait loci (QTL) mapping approach, with different resistant sources and isolates. We are trying to develop the molecular marker systems for detect all 8 CR resistant genes. Especially for the study on the interaction between pathogens and CR loci which are not well understood until now, genome wide association studies are doing using the sequenced inbred lines of Chinese cabbage to detect the novel CR genes.

• Journal of Biosystems Engineering
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• v.29 no.5 s.106
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• pp.425-432
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• 2004

In harvesting rice and barley using combine, the inclination of the body caused by the irregular surface condition of the field and the soil sinking from the unbalanced weight during the grain collection used to make harvesting operation difficult and even impossible. To overcome such a problem hydraulic system far automatic and manual leveling control of a combine has been developed. The system was composed of the combine body and the hydraulic level control system mounted on it. The maximum height of ground clearance was set up to be 290mm. And the limit angle of the leveling control was set up to be $\pm7^{\circ}$. The proposed controller and hydraulic system was implemented to the prototype combine. The prototype combine was designed and built as a separable structure with the body and the track of it. This paper shows results of a specification and design testing with the hydraulic level control system far body of combine.

• Journal of Microbiology and Biotechnology
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• v.16 no.12
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• pp.1947-1953
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• 2006

There have been a number of studies of methods for recycling animal wastewater to provide new bioresources. In the present work, a marine algal culture medium, designated KEP II, was prepared by adding swine waste (3% v/v) fermented by soil bacteria to a dilution of f/2 culture medium (CT). When Phaeodactylum tricornutum was grown in batch culture in KEP II, the cells lasted long at the exponential phase producing the specific growth rate and biomass; the production of total amino acids and secondary metabolites rose up to 5-fold. It also substantially enhanced the maximum quantum yield of photo system (PS) II of P. tricornutum, greatly increased the level of thylakoid membranes containing PS, and stimulated the production of pyrenoids, including enzymes for $CO_2$ fixation in chloroplasts. KEP II should improve the cost efficiency of industrial mass batch cultures and the value of microalgae for long-term preservation of fresh aquaculture feed as well as production of anticancer and antioxidant agents. Specifically, a low-cost medium for growing the diatoms of aquaculture feed will be economically advantageous.

• Journal of Soil and Groundwater Environment
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• v.27 no.2
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• pp.87-98
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• 2022

This study aims to prepare a technical protocol for identifying the source of nitrate in water using nitrogen (δ¹⁵N) and oxygen (δ¹⁸O) stable isotope ratios. The technical processes for nitrate sources identification are composed of site investigation, sample collection and analysis, isotope analysis, source identification using isotope characteristics, and source apportionment for multiple potential sources with the Bayesian isotope mixing model. Characteristics of various nitrate potential sources are reviewed, and their typical ranges of δ¹⁵N and δ¹⁸O are comparatively analyzed and summarized. This study also summarizes the current knowledge on the dual-isotope approach and how to correlate the field-relevant information such as land use and hydrochemical data to the nitrate source identification.

• Journal of Korean Society of Forest Science
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• v.54 no.1
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• pp.60-67
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• 1981

The run-off experiment plots had been established for eight sets of plot comprising four treatments with two replications on $20^{\circ}$ slope land having the Wait-A-Bit Clay soil(locally known), at the Olive River Soil Conservation Centre, Trelawny in Jamaica. The location of plots was about 820 metres m.s.l. and sloped north-west. Each plot size was determined as $40m^2$ having 2.7 m wide and 15.8 m long along slope. All of the run-off soil and water were collected by using the receiving tanks through the collection troughs and conveyance pipes. These run-off materials were measured and sampled, dried and computed for determination of the soil loss from each treatment of plots. During the first period of experiment for about 10 month which was one crop-year cycle of yam crop, total amount of 1,295 mm rainfall received. The heaviest daily rainfall was recorded as 116.2 mm on August 5 followed by 100.4 mm on August 6, 1980. The soil sediment had been collected and analysed for eleven times during this experiment. Total amounts of soil sediment as over-dried weight by the treatment plot were estimated as 182 ton/ha from treatment I, 105 tons/ha from treatment II, 50 tons/ha from treatment III, 43 tons/ha from treatment IV respectively. It is recommendable at present that the treatment III and IV measure which treated with contour mounds with the hillside ditch and grass buffer strip should be adopt4ed for hillside farming particularly with yam cultivation in Jamaica.

• Applied Biological Chemistry
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• v.42 no.2
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• pp.99-104
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• 1999

Soil actinomycetes of 703 strains were isolated from 25 sampling points in Cheju Island using 4 different media. Arginine glycerol salts agar containing soil extract was found to be the best medium for the isolation of soil actinomycetes. Soil samples from pasture land showed higher population and diversity of the actinomycetes than those from citrus field, forest, island, hill or valley. When the antibacterial activity of the 526 isolates was tested against three bacterial strains, Escherichia coli, Staphylococcus aureus and Pseudomonas solanacearum the frequency of the isolates with antibacterial activity varied much depending upon the media used for isolation and cultivation. BL106Ba, one of the 10 isolates that showed antibacterial activity against all the above 3 test strains, was chosen based upon the pH and heat stability of its antibacterial metabolites, and was identified as Streptomyces sp. based upon its cultural, morphological and physiological characteristics. The partially purified white crystalline substance obtained from the culture supematant of BL1063a through cation exchange chromatography(AG MP-50) and three times consecutive gel filtration(Sephadex G-10) showed high antimicrobial activity against gram positive and negative bacteria, but low activity against yeasts. The partially purified substance was found to contain at least four different compounds with antibacterial activity by both thin layer chromatography and high performance liquid chromatography.

• Journal of the Korean GEO-environmental Society
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• v.7 no.1
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• pp.29-39
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• 2006

Recently, Korea brings to remarkable levels about industrialization, modernization, population and development of technology. Especially, the rapidly growing from this technology has increased the burden on existing industrial waste landfills. The purpose of this research is to existing knowledge base of landfill cover liner behavior during periods freeze/thaw. Although these tests have been invaluable in clarifying the problem of freeze/thaw, extending the results of such experimental studies to prototype landfills are questionable. For this investigation, the author utilized a large scale laboratory simulation allowing inclusion of the field depth of the cover systems, layered soil profiles, rainfall simulation, a cold climate and boundary conditions similar to those encountered in the landfill. And the soil materials used stabilized soils (mixed clays, cements, and minerals) instead of clays. The bottom liners are made up of drainage layer (30cm), stabilized layer (75cm), and leach collection layer (60cm). The stabilized layers are made up of supporting layer (45cm) and impermeable layer (30cm) - consisted of $P_A$ and $P_B$ layer.

• Korean Journal of Medicinal Crop Science
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• v.25 no.2
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• pp.102-107
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• 2017

Background: The present study was conducted to investigate the fatty acid content and habitat conditions of Glehnia littoralis in the Korean midwest region. Methods and Results: The emergence area of G. littoralis was 36.1 m from the coastline at an elevation of 4.2 m and slope of 4.3%. The soil of the habitat was sandy, the pH was 8.4, organic matter content was 0.4%, $P_2O_5$ content was 9.1 mg/kg and the content of K, Ca, Mg and Na were 0.09, 9.31, 0.43 and $0.23cmol^+/kg$, respectively. Other plants growing in this area with G. littoralis included 16 taxa. Plant with high coverage and frequency were Carex kobomugi, Elymus mollis, Imperata cylindrica var. koenigii, Lathyrus japonicus, Calystegia soldanella and Carex pumila. In the seeds of G. littoralis, the maximum fatty acid content was 65.5% oleic acid, 28.7% linoleic acid, 4.3% palmitic acid, 1.5% stearic acid, and 0.2% linolenic acid. Conclusions: In G. littoralis of Korean midwest, the scope of collection and cultivation for medicinal purposes was broad, because differences in associated vegetation, soil composition, and fatty acid content owing to habitat conditions was low.

• Journal of Korean Society of Disaster and Security
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• v.7 no.2
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• pp.1-8
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• 2014

Smart Pole Measurement System was constructed with not only the core sensors of a GNSS receiver, a TRS sensor and a soil moisture sensor but supplementary installation of power supply and radio communication for monitoring steep slope sites. Also a data processing software for displacement extraction and visualization was developed. Smart Pole Measurement sensor is composed of a GNSS antenna at the top of the pole, a TRS sensor and a gyro sensor vertical below right of the antenna and a soil moisture sensor at the bottom of the pole. The sensor combination extracts not only ground combination in real time but transltion, slide, settlement and soil moisture content. This measuring/monitoring system which cosists of data receiving part, data collection/transfer part and data processing part was built to exercise their functions and then test measuring/monitoring was conducted by introducing artificial displacement and the results were analyzed to evaluate field applicability.

• Journal of Korea Soil Environment Society
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• v.4 no.1
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• pp.69-73
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• 1999

The study was performed to examine the elimination capacity of impurity on the fiberglass wicks to exert a suction of Passive capillary samplers (PCAPS) on soil water. This impurity affects the capillary properties of the wick and the chemical properties of the samples collected. To determine the need, capillary rise and moisture contents were measured after the wicks were cleaned by four methods : combustion, acetone extraction, detergent washing, and uncleaning. The wicks were made with fibers produced by PPG Industries and Manville Company, U.S.A. Results showed that wicks made from PPG fibers lost up to 3.4% of original mass during combustion while Manville fiber wicks lost only up to 0.6%. These losses are assumed to have been due to combustion of organic compounds applied by the manufacturers. All cleaning methods had higher capillary rise than obtained with uncleaning method Combustion at 40$0^{\circ}C$ for 4 hours was the best cleaning method. removing 98 to 100% of impurities.