• 한국자원식물학회지
• /
• 제8권2호
• /
• pp.183-188
• /
• 1995

땅두릅 여룹철 연화재배(軟化栽培) 가능성(可能性)을 검토(檢討)하기 위하여 흑색(黑色) PE와 은색(銀色) 단섬유불직포(短纖維不織布)를 이용하여 5월 정식(定植)을 포함하여 6월, 7월, 8월 정식기(定植期)에 대하여 시험(試驗)한 결과(結果)는 다음과 같다. 1. 수확소요일수(收穫所要日數)는 5월 정식(定植)에서 20일(日)이고 7월, 8월 정식(定植)에서는 15일로 단축(短縮)되었으나 생육기간중(生育期間中)의 적산온도(積算溫度)는 $400^{\circ}C$ 범위이었다. 2. 7월 정식(定植)에서 땅두릅 연화수(軟化莖)의 수량(收量)은 고온(高溫)으로 인한 연부현상(軟腐現狀)으로 낮은 경향(傾向)이었다. 3. 각(各) 부위별(部位別) 경도(硬度)는 상위부(上位部)가 연(軟)하고 하위부(下位部)가 단단한 경향(傾向)이고, 저온저장(低溫貯藏) 7일후(日後)에도 신선도(新鮮度)는 유지(維持) 되었다. 4. 5월, 6월 정식재배시(定植栽培時) 18,000천원/ha 이상(以上)의 소득(所得)을 얻을 수 있었다.

• 대한수의학회지
• /
• 제29권2호
• /
• pp.123-128
• /
• 1989

Demi-embryos were successfully produced by bisection of ICR mouse embryos at preimplantation stages. They were microsurgically bisected using a microsurgical blade attached to a micromanipulator after pretreatment with 0.5% pronase in PBS for two minutes or not. Embryos with softened zona pellucida were more easily bisected and less damaged than intact embryos. The highest success rate in bisection has been achieved by selecting blastocysts(94.1% in success rate with intact blastocysts and 100% in success rate with zona softened blastocysts). Demi-embryos without zona pellucida were cultured in D-PBS or M-16 medium at $37^{\circ}C$, 5% $CO_2$ in air for 72 hours for 2-cell stage embryos, 48 hours for 4-to 8-cell stage embryos, 24 hours for morula stage embryos and 6~12 hours for blastocyst stage embryos. For the in vitro culture of 2-cell stage embryos, $100{\mu}M$ 2Na-EDTA was added to the media. M-16 medium was better for the in vitro development of mouse embryos than PBS, and PBS is not considered to be suitable for long-term culture of embryos, especially at early stage of cleavage. In M-16 medium, developing rate of demi-embryos of which pair underwent development to form eublastocysts was 15.8% at 2-cell stage, 16.8% at 4-cell stage, 38% at 8-cell stage, 89.6% at morula stage and 94.4% at blastocyst stage, respectively. The more rapid and efficient production of demi-embryos and higher viability after bisection can be expected by softening zona pellucida with pronase and by selecting morulae or blastocysts rather than embryos at early stage of cleavage.

• 한국생물공학회:학술대회논문집
• /
• 한국생물공학회 2005년도 생물공학의 동향(XVI)
• /
• pp.31-32
• /
• 2005

Neoagaro-oligosaccharides are produced only by enzymatic degradation of agarose by ${\beta}-agarase.^{1)}$ Neoagaro-oligosaccharides inhibit the growth of bacteria, slow the rate of degradation of starch, are used as low-calorie additives to improve food quality, and have macrophage-stimulating activity. Furthermore, neoagarobiose is a rare reagent that has both moisturizing effect on skin and whitening effect on melanoma $cells.^{2)}$ An agar-degrading marine bacterium was isolated from the sea water at the northeast coast in Cheju island, Korea. The strain was gram negative, aerobic, and motile rod. The 16S rRNA of the strain had the closest match of 98% homology, with that from Agarivorans albus. On the basis of several phenotypic characters and a phylogenetic analysis, this strain was designated Agarivorans sp. JA-1. In solid agar plate, Agarivorans sp. JA-1 produced a diffusible agarase that caused agar softening around the colonies. Agarivorans sp. JA-1 was cultured for 36 hr in marine broth 2216 (Difco, USA) and the supernatant that containing an extracellular ${\beta}-agarase$ was prepared by centrifugation of culture media. The enzyme exhibited relatively strong activity at $40^{\circ}C$ and was stable up to $60^{\circ}C$. Using PCR primers derived from the ${\beta}-agarase$ gene of Vibrio sp., the gene encoding ${\beta}-agarase$ from Agarivorans sp. JA-1 was cloned and sequenced. The structural gene consists of 2931 bp encoding 976 amino acids with a predicted molecular weight of 107,360 Da. The deduced amino acid sequence showed 99% and 34% homology to $agaA^{2)}$ and $agaB^{2)}$ genes for ${\beta}-agarase$ from Vibrio sp., respectively. The expression plasmid for ${\beta}-agarase$ gene of Agarivorans sp. JA-1 is being constructed and the recombinant enzyme will be biochemically characterized.