• Parasites, Hosts and Diseases
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• 제53권2호
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• pp.219-222
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• 2015

Trichinellosis transmission to humans via the consumption of reptile meat is rare worldwide. In Korea, however, 2 such outbreaks, possibly via consumption of soft-shelled turtle meat, have occurred in 2 successive years. In 17 August 2014, 6 patients were admitted to Wonju Severance Christian Hospital complaining of myalgia, fever, and headache. Eosinophilia was the indication of the initial laboratory results, and they were eventually diagnosed as trichinellosis by ELISA. All of the patients worked at the same company and had eaten raw soft-shelled turtle meat at a company dinner 10 days prior to their admission. They were treated with albendazole for 2 weeks, upon which all of their symptoms disappeared. This is the 8th report on human trichinellosis in Korea, and the second implicating raw soft-shelled turtle meat.

• 수산해양교육연구
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• 제29권3호
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• pp.702-710
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• 2017

Recently, in many reptiles (14 genera of turtles in five families), common characteristics of incubation temperatures are known to determine the sexes of hatchlings in many species of turtles, including the map turtles, painted turtles and snapping turtles, emys turtle, etc. According to many researcher's reports, in general, incubation at $25^{\circ}C$ (cooler temperatures) produces all or mostly males, however, incubation at $31^{\circ}C$ (higher temperatures) or higher produces all or mostly females. Exceptionally, even cooler temperature ($20^{\circ}C$) produce females, they produced all or mostly females. Accordingly, it is well-known that incubation temperature is the sex determining agent in these turtles. However, this paper presents study of the sex ratio and nest ecology in natural spawning nest: Observations on hatching sex ratios of eggs collected from natural nests of T. sinensis are similar to a previous report of the same genus Trionyx in the soft-shelled turtles. However, this genus (or species) showed some different phenomena to other kinds of turtles such as various kinds turtles mentioned above. After collection of naturally spawned eggs (17 eggs of T. sisnensis) on the natural nests, a laboratory experiment by the constant incubation temperatures was conducted with natural fluctuating soil temperatures in the natural nest with the soft-shelled turtle, T. sinensis. And also laboratory experiments were conducted using constant incubation temperatures of $25^{\circ}C$ (cooler temp.) and $30^{\circ}C$ (higher temp.) with the turtle, T. sinensis. Exceptionally, it was confirmed that the first and second incubation temperatures can't control sex-determination in the freshwater soft-shelled turtle, T. sinensis. The sex ratio approximated 1:1 (${\chi}^2=0.06$, P>0.05 (the Ist experiment). And the sex ratio approximated 1:1 independently of incubation temperature (${\chi}^2=0.33$, P>0.05 (the 2nd experiment). Consequently, temperature has no effect on sex determination in the genus Trionyx in a soft-shelled turtle.

• Parasites, Hosts and Diseases
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• 제51권2호
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• pp.219-221
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• 2013

Reptiles, unlike mammals, have been considered to be unsuitable hosts of Trichinella spp., though larvae have been detected in their muscles and human outbreaks related to their consumption have, in fact, occurred. Herein we report 2 Korean cases of trichinosis, possibly transmitted via consumption of reptile meat. Both patients suffered from myalgia, headache, and facial edema. Laboratory examinations revealed leukocytosis with eosinophilia (54% and 39%, respectively) and elevated creatinine phosphokinase. ELISA was performed under the suspicion of trichinosis, showing positivity at the 29th and 60th day post-infection. Since they had consumed raw soft-shelled turtle meat, turtle was strongly suggested to be an infection source of trichinosis in Korea next to the wild boar and badger.

• Fisheries and Aquatic Sciences
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• 제10권4호
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• pp.226-229
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• 2007

Survival, tag retention and tag readability were compared among the control and three treatment groups of soft-shelled turtles, Pelodiscus sinensis Crother, 2000 (mean body $weight{\pm}SD$: $182.6{\pm}13.7\;g$), marked with visible implant fluorescent elastomer (VIFE) tags for 16 months. Mortality 4 to 16 months after tagging was attributed to collection and handling stress rather than to the tagging itself. Tags applied to the web surface between the fourth and fifth dactyl of the hindfoot appeared to have the highest retention rates, while adipose eyelid tagging had high tag readability but a high loss rate. We conclude that in soft-shelled turtles, the most suitable region for VIFE tagging is on the web surface between the fourth and fifth dactyls of the hindfoot.

• 한국양식학회지
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• 제20권1호
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• pp.60-64
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• 2007

양식 자라, Pelodiscus sinensis를 사용하여 진동 스트레스에 노출될 때 나타나는 생리적 반응의 단기적 지표로서 산소소비량(호흡)과 암모니아 배설량 변화를 조사하였다. 자라는 대형(갑장: $16{\sim}20\;cm$, 체중: $700{\sim}1,000\;g$)와 중형(갑장: $12{\sim}16\;cm$, 체중: $300{\sim}600\;g$)으로 구분하여, 1.5 ton 크기의 FRP 수조에 20마리씩 수용하였다. 진동 자극은 자체 제작한 진동기(vibrator)에 타임머를 연결하여, 하루 중 주간($08:00{\sim}18:00$)에만 2시간 간격으로 15분씩 28일 동안 매일 스트레스 자극을 주었다. 진동의 크기는 $45.0{\sim}68.2$(평균 56.6) dB(V) 였으며, 대조군 자라는 진동의 영향이 없는 별도의 사육수조에서 정상적으로 사육하면서 실험에 사용하였다. 연구결과, 진동 자극은 자라에 상당한 스트레스 요인으로 작용한 것으로 나타났다. 산소소비량 및 암모니아 배설량 등에서 체내 항상성이 스트레스 자극 10일 이후 생리활성도가 50% 이하로 감소하여 항상성 유지를 위한 모든 에너지를 소비하여 생리적 피폐기로 폐사직전인 것으로 나타났다. 자라는 다른 수산 생물과는 다르게 스트레스 상황에 처해 있을 때, 체외로 나타나는 징후가 뚜렷하지 않아 본 연구에서 나타난 산소소비와 암모니아 배설량은 자라의 생리적 활성도를 조사하는데 기초자료로 유용하게 사용될 것으로 판단된다.

• 한국양식학회:학술대회논문집
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• 한국양식학회 2006년도 수산관련학회 공동학술대회 발표요지집
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• pp.306-307
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• 2006

• 한국발생생물학회지:발생과생식
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• 제21권1호
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• pp.101-110
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• 2017

On June 14, 2008 (the first experiment) and July 24, 2008 (the second experiment), the shores of the Boseong River and the sandy beaches, Seokgok-myun, Moksadong-myun, Gokseong-gun in Jeollanam Province were investigated and a total of 29 soft-shelled turtle (Tryonyx sinensis) eggs in the natural spawning nest eggs were collected (13 eggs were collected in the first experiment and 16 eggs in the second experiment). The temperatures in the natural spawning nests were $25.9-36.9{\pm}0.5^{\circ}C$, the depth of the eggs was $5.2-7.5{\pm}0.5cm$ as the distance of the average $6.4{\pm}0.5cm$. 29 eggs were scattered at least 0.2 cm interval. Artificial incubation of 29 eggs was conducted in artificial nest boxes in thermo-plastic composition of the incubator, and then incubated at $26.5-35.5{\pm}0.5^{\circ}C$, and an average constant temperature was $31.2-32.1{\pm}1.0^{\circ}C$. The incubation days ranged from 53 to 55. In case of most turtles, incubation at $31^{\circ}C$ (higher temperatures) generally produces all or mostly females, while incubation at $25^{\circ}C$(cooler temperatures) produces all or mostly males. Exceptionally, in case of genus Trionyx, the sex ratio of female : male of T. sinensis of a freshwater soft-shelled turtle was approximately 1:1, which differs from other genera of turtles and makes T. sinensis Strauch only turtles presently known to lack temperature-dependent sex determination.

• 한국발생생물학회지:발생과생식
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• 제13권1호
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• pp.43-49
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• 2009

We examined the effects of the vibration stress on cortisol secretion and hematological characteristics in soft-shelled turtle, Pelodiscus sinensis. For the stressed group vibration of $45{\sim}78 dB(V)$ from electric vibrator applied for 30 min with 2-h intervals during daytime ($08:00{\sim}18:00$) up to 28 days. Using the blood samples collected from ten turtles held once a week after vibration stress, we measured hematocrit, hemoglobin, red blood cells, cortisol, glucose, lactic acid, osmolality, $Na^+,\;K^+,\;Cl^－$, aspartate aminotransferase (AST), and alanine aminotransferase (ALT). The results have showed that P. sinensis received vibration stress exhibit the 'typical' stress-induced physiological responses (cortisol, glucose, lactic acid, osmolality, ions, hematocrit and hemoglobin) induced by vibration stress. Our data suggested that chronic vibration stress caused substantial stress in the animal, and in particular, the persisting elevated levels of AST and ALT would be highly correlated with the adverse effects of the stress. The high hematological characteristics during entire experimental period showed that the P. sinensis could not adapt to chronic stimuli provoked by vibration stress.

• 한국양식학회:학술대회논문집
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• 한국양식학회 2006년도 수산관련학회 공동학술대회 발표요지집
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• pp.235-236
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• 2006

• Fisheries and Aquatic Sciences
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• 제9권3호
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• pp.107-114
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• 2006

A dermal sarcoma was found in a freshwater, soft-shelled turtle Pelodiscus sinensis. The neoplasm consisted of proliferating fibrous tissue and extended from the dermis. The overlying epidermis was hyperplastic and partially folded. The deeper dermis and hypodermis contained three large, discrete necrotic foci of -10 mm diameter. Numerous eosinophilic granule cells and macro phages surrounded the necrotic areas. A mixed population of cells with nuclear pleomorphism was observed between the papillary layers of vessels. This area also had regions of different histological structures: (l) regularly arranged, spindle-shaped cells with compact nuclei in a fine-fibrillar matrix; (2) haphazardly arranged cells ($\leq$ 23 11m diameter) with ovoid, highly hypertrophic, faintly stained nuclei; and (3) cells (3.6-5.8 11m diameter) with irregularly shaped nuclei and marginal condensed chromatin in a myxomatous matrix. Some mitotic figures, binucleate cells, and multinucleate giant cells of up to 50 11m in length were also found. Flow cytometry of propidium iodide-stained cells yielded different histograms for the normal skin and the skin (primarily epidermis) and fibrous dermis of the tumor, indicating DNA heterogeneity in the dermal portion of the tumor. The ploidy indices for the dermal cells were 1.91 and 0.78, as compared to normal cells.