• Archives of Reconstructive Microsurgery
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• 제11권2호
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• pp.141-145
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• 2002

Rectus abdominis muscle free flap is widely used for breast reconstruction and soft tissue defect in lower leg but donor-site morbidities such as abdominal wall weakness, hernia, bulging are troublesome. Recently, to minimize donor-site morbidity, there has been a surge in interest in deep inferior epigastric perforator(DIEP) free flap preserving the anatomy of rectus abdominis muscle, fascia, and motor nerve. Between August of 1995 and September of 2002, topographic investigation of DIEP was performed during the elevation of 97 cases of TRAM free flap and 5 cases of DIEP free flap. There were 84 cases of breast reconstructions, 12 cases of lower leg reconstructions, and 6 cases of head and neck reconstruction. We could observe total 10 to 12 perforators on each rectus abdominis muscle below umbilicus. Among these, the numbers of large perforators(>1.5mm of diameter) were mean 2.1 in lateral half of rectus abdominis muscle, mean 1.2 in medial half, and mean 0.5 in linea alba and paramedian. DIEP free flap provides ample amount of well vascularized soft tissue without inclusion of any rectus abdominis muscle and fascia and minimizes donor-site morbidity. One perforator with significant flow can perfuse the whole flap. For large flap, a perforator of the medial row provides better perfusion to zone-4 than one of lateral row and, if diameter of perforator is small, $2{\sim}3$ perforators can be used. According to the condition of recipient-site, thin flap can be harvested. As DIEP free flap has many advantage, perforator topography will be useful in increasing clinical usage of DIEP free flap.

• 한국임상수의학회지
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• 제32권2호
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• pp.200-204
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• 2015

12년령 중성화 수컷 시추가 변비와 배변곤란 증상으로 내원하였다. 방사선 사진상 결장이 팽대되어 있으며 천추 수준에서 연부조직밀도의 종괴가 결장을 복측으로 압박하는 것이 관찰되었다. 컴퓨터 단층촬영상 종괴는 균일한 연부조직 정도의 감약성을 보이며 변연이 뚜렷하고 전이소견을 보이지 않았다. 동적 컴퓨터 단층촬영상 종괴는 촬영간 지속적인 낮은 조영증강을 보였으며 관류성 및 모세혈관 투과성은 둔부근육과 비교하여 낮은 것으로 평가되었다. 영상진단학적 검사상 종괴는 신생혈관 발달이 약한 골반강 내 양성 종양으로 잠정진단되었다. 종괴는 둔성분리를 통한 외과적 절제로 제거되었으며 조직검사결과 평활근종으로 진단되었다.

• Archives of Reconstructive Microsurgery
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• 제6권1호
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• pp.96-102
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• 1997

The gracilis that is frequently used as a donor of free muscle trasfer is appropriate in the muscular shape and vascular position. This muscle is belonged to the second type of muscle group by the classification of the pattern of muscular nutrient vessel. The adductor branch or first perforating branch of deep femoral artery which supplies the proximal 1/3 of this muscle is a dominant one and this is used for the microscopic anastomosis of muscle or musculocutaneous flap. The minor vascular pedicles which enter the distal 1/3 of this of this muscle are branches of the superficial femoral artery and it is 0.5mm in diameter, 2cm in length with two venae comitantes. These minor pedicles supplies distal half of the gracilis muscle. This island musculocutaneous flap using distal vascular pedicle can be used to cover the defect of soft tissue around the distal femoral supra-condylar area, knee joint and proximal tibial condyle area which cause limitation of motion of knee joint, or in the cases that usual skin graft is impossible. The important operative procedure is as follows; The dissection is carried proximally and distally and the entire gracilis muscle including proximal and distal pedicle is completely dissected. After temporary blocking of the proximal vascular pedicle, the adequate muscle perfusion by the distal pedicle is identified and it is rotated to the recipient site around knee joint. The advantages of this procedure are simple, no need of microscopic vascular anastomoses and no significant functional loss of donor site. Especially in the cases of poor condition of the recipient vessel, this procedure can be used effectively. From 1991 to 1996, we performed 4 cases; complete survival of flap in 3 cases and partial survival of flap with partial necrosis in 1 case. This procedure is though to be useful in the small sized soft tissue defect of distal femoral supra-condylar area, knee joint and proximal tibial condylar area, especially in the defect of anterior aspect which expected to cause limitation of motion of knee joint due to scar contracture. But the problems of this procedure are the diameter of distal vascular pedicle is small and the location of distal vascular pedicle is not constant. To reduce the failure rate, identify the muscular perfusion of distal vascular pedicle after blocking the proximal pedicle, or strategic delay will be helpful.

• Journal of Periodontal and Implant Science
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• 제30권2호
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• pp.257-277
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• 2000

New techniques for regenerating the destructed periodontal tissue have been studied for many years. Current acceptable methods of promoting periodontal regeneration are basis of removal of diseased soft tissue, root treatment, guided tissue regeneration, graft materials, and biological mediators. Platelet Rich Plasma has been reported as a biological mediator which regulates activities of wound healing progress including cell proliferation, migration, and metabolism. The purpose of this study is to evaluate the effects of using the Platelet Rich Plasma as a regeneration promoting agent for furcation involvement defect. Five adult beagle dogs were used in this experiment. The dogs were anesthetized with Ketamin HCl(0.1 ml/kg, IV)and Xylazine hydrochloride($Rompun^{(R)}$, Bayer, 0.1 ml/kg, IM) and conventional periodontal prophylaxis were performed with ultrasonic scaler and hand instruments. With intrasulcular and crestal incision, mucoperiosteal flap was elevated. Following decortication with 1/2 high speed round bur, degree II furcation defect was made on mandibular third(P3), forth(P4) and fifth(P5) premolar, and stopping was inserted. After 4 weeks, stopping was removed, and bone graft was performed. Ca-P was grafted in P3(experimental group I), Combination of Ca-P and plasma rich platelet were grafted in P4(experimental group II), and P5 was remained at control group.Systemic antibiotics(gentamicin sulfate)and anlgesics(phenyl butazone) were administrated intramuscular for 2 weeks after surgery. Irrigation with 0.1% Chlorhexidine Gluconate around operate sites was performed during the whole experimental period except one day immediate after surgery. Soft diets were fed through the whole experiment period. After 4, 8 weeks, the animals were sacrificed by perfusion technique. Tissue block was excised including the tooth and prepared for light microscope with Gomori's trichrome staining. At 4 weeks after surgery, there were rapid osteogenesis phenomenon on the defected area of the Platelet Rich Plasma plus Ca-P BBP group and early trabeculation pattern was made with new osteoid tissue produced by activated osteoblast. Bone formation was almost completed to the fornix of furcation by 8 weeks after surgery. In conclusion, Platelet Rich Plasma can promote rapid osteogenesis during healing of periodontalregeneration.

• Archives of Reconstructive Microsurgery
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• 제7권1호
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• pp.62-67
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• 1998

전북대학교병원 정형외과에서 1994년 6월부터 1998년 3월까지 하퇴부 원위 1/3 및 족부에 시행하였던 박근 유리조직 이식술 12례에 대하여 최소 6개월부터 최고 4년 9개월까지 추시하여 다음과 같은 결과를 얻었다. 1. 하퇴부 원위 1/3의 손상원인은 개방성 골절에 의한 뼈 및 연부조직노출이 4례(33.3%)였으며, 골절수술후 연부조직 괴사로 인한 2차 뼈 및 내고정물노출이 2례(16.7%)였고, 족부의 손상원인은 압궤손상 5례(41.7%), 골절치료시 발뒤꿈치의 압박괴사 1례(8.3%)였다. 2. 수여혈관은 하퇴부 원위 1/3에서는 전경골 동맥이 4례, 비골동맥과 족배동맥이 각각 1례였으며, 족부의 수여혈관은 족배동맥이 4례, 후 경골동맥이 2례였다. 수여정맥은 2개를 봉합함을 원칙으로 하였으나 하퇴부 원위 1/3에서 총 6례중 3례, 족부에서도 총 6례중 3례에서만 2개의 수여정맥 봉합술이 가능하였다. 3. 총 12례중 11례(91.7%)에서 술후 3주까지 관류(perfusion)가 가능하여 성공하였으며, 피부 이식술은 술 후 평균 22일만에 시행하였고, 재활운동은 술후 평균 32일만에 가능하였다.

• Journal of Trauma and Injury
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• 제28권3호
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• pp.158-169
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• 2015

Purpose: Frostbite can affect still soldiers. Initial clinical manifestations are similar for superficial and deep frostbite, so early treatment is identical. It is under-estimated by physicians. We try to identify the challenges of managing these complex tissue injuries. Methods: A retrospective analysis of 84 patients hospitalized at AFCH from 2009 to 2015 was conducted. We investigated differences of epidemiological characteristics, identification of soft tissue injury, treatment and complications between superficial (SF: 43; 51.2%) and deep (DF: 41; 48.8%) frostbite. Results: The major (94.0%) developed frostbite in dry circumstances (89.3%). Wet circumstances (66.7%) were more susceptible to DF rather than dry (46.7%). The 38 (45.2%) arrived to specialist within 7days. Most prone sites were feet, followed by hands. Toes had more deep injuries. DF presented more increased levels of ALT, CPK, CKMB, CRP. The bone scan of W+S+ was 48.3%, 87.1% and W+S- was 20.7%, 12.9%, respectively. The treatment resulted in improved or normalized perfusion scan with matching clinical improvement. It was a good tool to assess treatment response. Eighteen normal and 8 stenotic type of PCR resulted in normal with matching clinical improvement. One continuous obstructive waveform led to minor amputation. Twelve underwent both PCR and MRA. Among 6 normal PCR, 5 showed normal and one stenosis in MRA. All 5 stenosis and one obstruction showed the same findings in MRA. It was a good tool to evaluate vascular compromise. They were treated with rapid rewarming (11.6%, 22.0%), hydrotherapy (16.3%, 29.3%), respectively. Six (14.6%) underwent STSG, 2 (4.9%) had digital amputation in DF. Berasil, Ibuprofen, Trental were commonly administered. PGE1 was administered selectively for 6.8, 10.8 days, respectively. Raynaud's syndrome (16.3%), CRPS (4.7%), LOM (14.6%) and toe deformity (4.9%) were specific sequelae. Conclusion: We should recommend intensive foot care education, early rewarming and evacuation to specialized units. The bone scanning and PCR should allow for a more aggressive and active approach to the management of tissue viability.

• Archives of Plastic Surgery
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• 제44권4호
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• pp.261-265
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• 2017

Periauricular paresthesia may afflict patients for a significant amount of time after facelift surgery. When performing face and neck lift surgery, temple and posterior auricular flap dissection is undertaken directly over the auriculotemporal, great auricular, and lesser occipital nerve territory, leading to potential damage to the nerve. The auriculotemporal nerve remains under the thin outer superficial fascia just below the subfollicular level in the prehelical area. To prevent damage to the auriculotemporal nerve and to protect the temporal hair follicle, the dissection plane should be kept just above the thin fascia covering the auriculotemporal nerve. Around the McKinney point, the adipose tissue covering the deep fascia is apt to be elevated from the deep fascia due to its denser fascial relationship with the skin, which leaves the great auricular nerve open to exposure. In order to prevent damage to the posterior branches of the great auricular nerve, the skin flap at the posterior auricular sulcus should be elevated above the auricularis posterior muscle. Fixating the superficial muscular aponeurotic system flap deeper and higher to the tympano-parotid fascia is recommended in order to avoid compromising the lobular branch of the great auricular nerve. The lesser occipital nerve (C2, C3) travels superficially at a proximal and variable level that makes it vulnerable to compromise in the mastoid dissection. Leaving the adipose tissue at the level of the deep fascia puts the branches of the great auricular nerve and lesser occipital nerve at less risk, and has been confirmed not to compromise either tissue perfusion or hair follicles.

• Investigative Magnetic Resonance Imaging
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• 제11권1호
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• pp.10-19
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• 2007

기존 MRI, 즉 T1 강조-, T2 강조-, 확산-, 관류-, 기능적-, 등의 영상법은 조직의 물리적 파라미터 그리고 기능적 특성을 알려주는 역할을 한다. 본 종설에서는 최근 관심이 높아지고 있는 영상기법의 하나로 MRE (Magnetic Resonance Elastography, 자기공명탄성법)를 소개하고자 한다. MRE는 기존의 물리적, 기능적 측정을 벗어나 조직의 기계적 특성에 관한 정보를 제공해준다는 면에서 MRI를 이용한 새로운 modality로서의 가능성을 시사해 준다. 예로부터 진단의 가장 기초적인 방법중 하나로서 촉진을 이용하여 조직의 경도를 가늠하여 왔다. MRE는 조직의 경도를 MRI를 이용하여 객관적으로 수치화해준다. MRE 임상실험을 성공적으로 수행하기 위해서는 몇 가지 하드웨어와 소프트웨어(트랜스듀서, 펄스대열, 영상처리 알고리즘)가 구비되어야 한다. 트랜스듀서는 인체에 진동을 전달해주는 부분으로서 MRE 응용을 가능하게 하는 핵심적인 역할을 한다. 따라서 MRI 시스템의 자기장과 인체의 골격, 피부와 트랜스듀서 접촉면의 압력, 마찰을 고려하여 제작하여야 한다. 트랜스듀서를 통해서 인체 내부에 진동이 전달되고 있으면 최적의 영상을 얻기 위하여 고려되어야 할 사항이 펄스대열을 조정하는 것이다. 마지막으로 여러 가지 물질에 대한 가정(등방성, 균질성, 비압축성)하에서 영상처리 알고리즘은 파동방정식(Helmholtz equation)으로 표현되며 이로부터 탄성도(Elasticity or Modulus)를 구할 수 있다. 본 종설에서는 이에 대한 리뷰 및 MRE를 이용한 응용분야에 대하여 살펴본다.

• Journal of Periodontal and Implant Science
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• 제26권1호
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• pp.27-46
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• 1996

The purpose of this study was designed to compare with the effects of 4 different surface active bioceramics on the healing process of alveolar bone defects in dogs. Artificial alveolar bone defects depth 4-6mm, width 3-4mm) were created with # 6 round bur at interproximal areas of maxillary canine, maxillary 2nd premolar, mandibular canine, and mandibular 3rd premolar. porous hydroxyapatite(Interpore $200^R$) , 45S5 bioglass, CJ4/lOC crystalline glass, and JJ crystalline glass were implanted in intrabony defects randomly. Experimental groups were divided into 4 categories according to its implant material. After implantation, all groups were examined postoperatively 4 weeks to 12 weeks. 3 dogs was selected randomly and sacrificed after vascular perfusion with 2.5% glutaraldehyde at every 4 weeks. Tissue blocks with surroundig alveolar bone and soft tissues were removed and immersed in formaldehyde/glutaraldehyde fixative. After 20 weeks decalcification with EDTA and formic acid, sections were made and observed under light microscope and transmission electron microscope. In all experimental groups, the encapsulation of inactive connective tissue was observed around graft particles in 4 weeks. As time elapsed, the thickness of surrounding connective tissue was decreased. Osteoconductive bone growth pattern was seen apparently in all groups. CJ4/lOC crystalline glass showed the most active bone formation until 8 weeks. 45S5 bioglass was, however, the most active in new bone formation at 12 weeks. Though there was difference in resorption rate among grafting materials, the size of graft particles was decreased gradually. 45S5 bioglass was resorbed faster than the others. On the other hand, porous hydroxyapatite was degraded most slowly. Phagocytosed particulate matters was observed in the cytoplasm of multinuclear multinuclear giant cell and macrophage under transmission electron microscope. The results suggested suggested that 45S5 bioglass and CJ4/lOC crystalline glass may have some enhanced reparative potential when compared to porous hydroxapatite in the treatment of periodontal defeds. JJ crystalline glass reguires a further investigation of the safety of its use.

• Journal of Periodontal and Implant Science
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• 제23권3호
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• pp.535-563
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• 1993

치주질환 진행의 최종적인 결과는 하반 지지조직의 소실로 치아상실을 초래하는 것이다. 이러한 치주질환의 이상적인 치유형태는 부착상실 예방을 비롯 상실된 조직의 재상 즉 신생 치조골과 백악질이 형성되고 두 조직사이에 치주인대가 재형성되는 것이다. 최근까지의 치주조직재생을 위한 처치법으로 이환된 병소부의 단순제거, 치관변위판막술, 약제의 치근면처리법, 조직유도재생술, 골전도물질 삽입, 골유도 혹 골형성물질 사용등 다양한 방법이 제시되었으나 아직 이상적인 치료법은 밝혀지지 않고 있다. 치아지지의 가장 중요한 역할을 하는 치조골의 재생에 대해 많은 연구들과 아울러 조직화학적 연구에서 Polypeptide Growth Factor(PGF) 가 다양한 종류의 세포증식과 이주 및 기질합성에 촉진효과가 있다고 하여 조직재생에 사용 가능성이 보고된 바 있었다. 이 PGF중 혈소판유래성장인자가 섬유아세포와 골아세포의 유사분열 및 단백질합성에 촉진작용이 있으며 조직재생에 영향을 미친다는 보고도 있었다. 본 연구의 목적은 총 8 마리의 실험동물을 이용하여 치근이개부병변을 형성한 후 혈소판유래성장인자를 처리하고 기존 조직결손부에 사용하였던 Tricalcium Phosphate(TCP) 와 콜라젠을 성장인자 함유매개체로 하여 이개부병변에 병용삽입한 경우 치유과정에 미치는 효과를 규명하여 임상적용의 가능성을 규명하고 실제 임상적용방법을 개발할 목적으로 실시하였다. 실험동물 Pentobarbital Sodium으로 전신마취를 시킨후, 초음파치석제거기등을 이용하여 구강위생을 청결하게 한 다음, 치은열구절개를 이용하여 전층판막을 형성하였다. 피질골과 치조골을 삭제하고 형성된 이개부 결손부에 치근면활택술을 시행하였으며, 구연산으로 치면처리 후, 계획된 재료를 삽입하고, 치관변위판막술과 유사한 형태의 봉합을 시행하였다. 실험동물을 2, 4, 8, 12 주에 관류고정과 아울러 회생시켜 악골을 채취하고 통법에 의해 후고정, 탈회, 탈수과정을 거쳐 파라핀으로 포매한 후 $7{\mu}m$두께로 절편하고 H & E 염색후 광학현미경으로 관찰하여 다음과 같은 결과를 얻었다. PDGF-BB 만 처리한 군에서는 2주 소견에서부터 결손부 전체에 걸쳐 활성도가 높은 조골세포들이 균일하게 분포하면서 이들로부터 생성된 골양조직이 기초적인 골소주의 형태를 이루고 있음이 관찰되었으며 그후 매우 빠른 골형성이 계속되어 8주 소견에서는 결손부 정상에 이르기까지 성숙된 치밀골이 채워져 있었다. 신생골형성의 전반적 형태는 치근면의 외형에 따라 치근이개부상단부로 형성되는 양상이었다. PDGF-BB 군에서 신생백악질의 형성은 2주소견 에서는 미약하였으나 4주이후 치근면에 수직으로 배열된 교원섬유들과 함께 균일한 두께로 형성되기 시작하여, 8주에 이르러서는 비 손상부위에서보다 더욱 두터운 신생백악질이 형성되었고 전형적인 샤피스씨 섬유가 완성되어 있음이 관찰되었다. PDGF-BB와 TCP를 병용한 경우 및 PDGF-BB와 콜라젠을 병용한 경우에서는 PDGF-BB군에 비해 신생골 및 신생백악질의 형성, 치주인대강의 발달이 상대적으로 미약하였으며 이러한 현상은 수술후 초기단계에서 더욱 두드러져 함유매개체로서의 기능을 기대하였던 이들 이식재들이 도리어 급속하게 분화 증식되는 세포들의 이동에 장애물로서 작용하는 것으로 나타났다. 결론적으로 PDGF-BB의 치근면 처리가 치근이 개부병변 치료에 있어 전반적으로 조직재생의 속도가 빠르고 그 치유양상도 시간경과에 따라 치주조직 고유형태로 진행됨이 관찰되어 동일 병소치료에 응용가능성을 확인하였다. 또한 차후 결손부에 주입방법과 성장인자의 관리법 및 적용량 그리고 적응중의 규명을 위해 연이은 연구가 있어야 할것으로 사료된다.