• Restorative Dentistry and Endodontics
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• 제33권6호
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• pp.545-552
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• 2008

본 연구의 목적은 NaOCl로 처리된 치수강 상아질에 total-etching adhesive system(single bond, 3M-ESPE, St Paul, MN, USA)을 적용할 때 미세인장결합강도에서 sodium ascorbate가 미치는 영향을 평가하는 것이다. 발치된 건전한 인간 대구치의 치수강 내면을 다음과 같이 처리하였다. 1군, 0.9% NaCl로 세척; 2군, 5.25% NaOCl로 세척; 3군, 5.25% NaOCl로 세척한 후 10% sodium ascorbate를 1분간 적용; 4군, 5.25% NaOCl로 세척한 후 10% sodium ascorbate를 1분간 적용하고 다시 10ml 물로 수세; 5군, 5.25% NaOCl로 세척한 후 10% sodium ascorbate를 5분간 적용; 6군, 5.25% NaOCl로 세척한 후 10% sodium ascorbate를 5분간 적용하고 다시 10ml 물로 수세; 7군, 5.25% NaOCl로 세척한 후 10% sodium ascorbate를 10분간 적용; 8군, 5.25% NaOCl로 세척한 후 10% sodium ascorbate를 10분간 적용하고 다시 10ml 물로 수세. 처리된 시편들은 total-etching adhesive system(Single bond)으로 처리한 후 composite resin(Z250)으로 충전하고, 미세인장강도를 측정하기 전 24시 간 동안 보관하였다. 분석 결과 NaOCl 처리 군(2군)은 다른 실험군들에 비해 유의성 있게 낮은 결합 강도를 보였다. NaCl 처리 군(1군)과 sodium ascorbate 처리 군(3-8군)은 유의성 있는 차이가 없었다. 이번 연구 결과는 NaOCl로 처리한 치수강 상아질의 Single bond에 대한 미세인장결합강도는 감소되며, 10% sodium ascorbate를 추가적으로 처리하였을 때 감소된 결합력이 회복됨을 보여주었다. Sodium ascorbate의 다양한 처리시간은 결합력에 영향을 미치지 않았다.

• Restorative Dentistry and Endodontics
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• 제34권6호
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• pp.515-525
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• 2009

본 연구에서는 치수강이 개방된 근관와동에 5.25% NaOCl용액을 각각 다른 시간 동안 적용시킨 뒤 접착레진에 의한 미세인장결합강도의 변화를 측정하였으며, 10% sodium ascorbate를 NaOCl 용액에 일정시간 노출시킨 상아질에 다시 10분 동안 적용하여 결합강도에 미치는 효과를 연구하고자 하였다. 연구결과 본 실험에서 상부 치수강 상아질과 하부 치수강 상아질에 대한 결합강도는 모든 실험군내에서 유의한 차이 (p > 0.05)를 보이지 않았다. 5.25% NaOCl 용액을 적용하지 않고 치수강 상아질에 접착시킨 대조군에 비해 20분간 적용시킨 실험군에서는 결합강도의 감소가 초래되었으나 통계적인 유의성 (p > 0.05)은 없었다. 그러나 5.25% NaOCl 용액을 40분과 80분 동안 치수강 상아질에 적용시킨 실험군에서는 대조군에 비해 유의하게 낮은(p < 0.05) 결합강도의 변화를 나타내었다. 5.25% NaOCl 용액을 40분동안 그리고 10% sodium ascorbate를 10분간 처리한 실험군에서는 유의한 결합강도의 회복을 나타내었다. 그러나 5.25% NaOCl 용액을 80분 동안 그리고 10% sodium ascorbate를 10분간 적용한 실험군에서는 대조군에 비해 통계적으로 유의한 차이(p < 0.05)를 보임으로써 여전히 결합강도가 회복되지 못하고 있음을 나타내었다. 5.25% NaOCl 용액을 5분간 적용한 뒤 10% sodium ascorbate를 10분간 적용한 실험군에서는 오히려 대조군에 비해 높은 결합강도(p < 0.05)를 보여줌으로써 높은 회복효과를 나타내었다.

• Restorative Dentistry and Endodontics
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• 제40권1호
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• pp.37-43
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• 2015

Objectives: Some antioxidants are believed to restore dentin bond strength after dental bleaching. This study was done to evaluate the influence of antioxidants on the bond strength of bleached bovine dentin. Materials and Methods: Thirty incisors were randomly assigned to 10 groups (two unbleached control and eight bleached groups:immediate bonding IB, 4 wk delayed bonding DB, 10% sodium ascorbate treated SA, 10% ${\alpha}$-tocopherol treated TP groups). Teeth in half of groups were subjected to thermal stress, whereas the remaining groups were not. Resin-dentin rods with a cross-sectional area of $2.25mm^2$ were obtained and microtensile bond strength was determined at a crosshead speed of 1 mm/min. Fifteen specimens were prepared for SEM to compare the surface characteristics of each group. The change in dentin bond strength from thermal stress and antioxidant treatment was evaluated using two-way analysis of variance (ANOVA) and Sheffe's post hoc test at a significance level of 95%. Results: The control group exhibited the highest bond strength values, whereas IB group showed the lowest value before and after thermocycling. The DB group recovered its bond strength similar to that of the control group. The SA and TP groups exhibited similar bond strength values with those of the control and DB groups before thermocycling. However, The TP group did not maintain bond strength with thermal stress, whereas the SA group did. Conclusions: Applying a 10% sodium ascorbate solution rather than 10% ${\alpha}$-tocopherol solution for 60 sec is recommended to maintain dentin bond strength when restoring non-vitally bleached teeth.

• Restorative Dentistry and Endodontics
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• 제39권2호
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• pp.95-103
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• 2014

Objectives: This study evaluated the effect of three antioxidizing agents on pullout bond strengths of dentin treated with sodium hypochlorite. Materials and Methods: Root canals of 75 single-rooted human teeth were prepared. Fifteen teeth were irrigated with normal saline for a negative control group, and the remaining 60 teeth (groups 2 - 5) with 2.5% NaOCl. The teeth in group 2 served as a positive control. Prior to post cementation, the root canals in groups 3 - 5 were irrigated with three antioxidizing agents including 10% rosmarinic acid (RA, Baridge essence), 10% hesperidin (HPN, Sigma), and 10% sodium ascorbate hydrogel (SA, AppliChem). Seventy-five spreaders (#55, taper .02, Produits Dentaires S.A) were coated with silica and silanized with the Rocatec system and ceramic bond. All the prepared spreaders were cemented with a self-adhesive resin cement (Bifix SE, Voco Gmbh) in the prepared canals. After storage in distilled water (24 h/$37^{\circ}C$), the spreaders were pulled out in a universal testing machine at a crosshead speed of 1.0 mm/min. Pull-out strength values were analyzed by one-way ANOVA and Tukey's HSD test (${\alpha}$ = 0.05). Results: There were significant differences between study groups (p = 0.016). The highest pullout strength was related to the SA group. The lowest strength was obtained in the positive control group. Conclusions: Irrigation with NaOCl during canal preparation decreased bond strength of resin cement to root dentin. Amongst the antioxidants tested, SA had superior results in reversing the diminishing effect of NaOCl irrigation on the bond strength to root dentin.

• Restorative Dentistry and Endodontics
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• 제39권4호
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• pp.303-309
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• 2014

Objectives: Hydrogen peroxide ($H_2O_2$) surface treatment of fiber posts has been reported to increase bond strength of fiber posts to resin cements. However, residual oxygen radicals might jeopardize the bonding procedure. This study examined the effect of three antioxidant agents on the bond strength of fiber posts to conventional and self-adhesive resin cements. Materials and Methods: Post spaces were prepared in forty human maxillary second premolars. Posts were divided into five groups of 8 each: G1 (control), no pre-treatment; G2, 10% $H_2O_2$ pre-treatment; G3, G4 and G5. After $H_2O_2$ application, Hesperidin (HES), Sodium Ascorbate (SA) or Rosmarinic acid (RA) was applied on each group respectively. In each group four posts were cemented with Duo-Link conventional resin cement and the others with self-adhesive BisCem cement. Push-out test was performed and data were analyzed using 2-way ANOVA and tukey's post-hoc test (${\alpha}=0.05$). Results: There was a statistically significant interaction between the cement type and post surface treatment on push-out bond strength of fiber posts (p < 0.001, F = 16). Also it was shown that different posts' surface treatments significantly affect the push-out bond strength of fiber posts (p = 0.001). $H_2O_2$ treated posts (G2) and control posts (G1) cemented with Duo-link showed the highest ($15.96{\pm}5.07MPa$) and lowest bond strengths ($6.79{\pm}3.94$) respectively. Conclusions: It was concluded that $H_2O_2$ surface treatment might enhance the bond strength of fiber posts cemented with conventional resin cements. The effect of antioxidants as post's surface treatment agents depends on the characteristics of resin cements used for bonding procedure.

• Restorative Dentistry and Endodontics
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• 제42권3호
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• pp.188-199
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• 2017

Objectives: This in vitro study evaluated the effect of dentin biomodifiers on the immediate and long-term bond strengths of a simplified etch and rinse adhesive to dentin. Materials and Methods: Flat coronal dentin surfaces were prepared in 120 extracted human molars. Teeth were randomly divided into 5 groups (n = 24) according to 5 different surface pre-treatments: No pre-treatment (control); 1M carbodiimide (EDC); 0.1% epigallocatechin-3-gallate (EGCG); 2% minocycline (MI); 10% sodium ascorbate (SA). After surface pre-treatment, adhesive (Adper Single Bond 2 [SB], 3M ESPE) was applied. Composite was applied into transparent plastic tubes (2.5 mm in diameter), which was placed over the bonded dentin surface. From each group, 10 samples were subjected to shear bond strength (SBS) evaluation at 24 hours (immediate) and remaining 10 samples were tested after 6 months (delayed). Additionally, 4 samples per group were subjected to scanning electron microscopic analysis for observation of resin-dentin interface. The data were statistically analysed with Shaperio-Wilk W test, 2-way analysis of variance (ANOVA), and post hoc Tukey's test. Results: At 24 hours, SBS of all surface pre-treatment groups were comparable with the control group, with significant differences found between EDC and SA groups only (p = 0.009). After 6 months storage, EDC, EGCG, and MI pre-treatments preserved the resindentin bond strength with no significant fall. Conclusions: Dentin pre-treatment with all the dentin biomodifiers except SA resulted in significant preservation of resin-dentin bond over 6 months storage period, without negatively affecting the immediate bond strength of the etch and rinse adhesive tested.

• Journal of Pharmaceutical Investigation
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• 제29권3호
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• pp.205-210
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• 1999

To formulate the parenteral delivery of a new cephalosporin derivative, 7-${\beta}$-[(2)-2-(2-arninothiazol-4-yl)-2methoxyiminoacetamido]- 3- [(2,3-cyclopenteno-4-carbamoyl-l-pyridinium)methyl]- 3-cephem-4-carboxylate sulfate( CKD604), the stability and solubility of CKD-604 in various aqueous media were investigated. The degradation kinetics of CKD-604 in aqueous solutions (ionic strength 0.1, pH 1-8) were studied at $37^{\circ}C$. The observed degradation rates followed pseudo first order kinetics. The pH-rate profile exhibited a minimum degradation rate at pH 5. The Arrhenius activation energy was 14.2 kcal/mol in pH 5 buffer solution. Excellent agreement between the cephalosporins' theoretical pH-rate profile and the experimental data indicated that the degradation pathway of CKD-604 could be predicted according to the general pathway of cephalosporins. The solubility of CKD-604 was 8.16 mg/ml at $25^{\circ}C$. To enhance the solubility and adjust the suitable pH, CKD-604 was solubilized by using sodium ascorbate, ascorbic acid and urea. The compositions were obtained to satisfy optimum pH and concentration, and the total amount of additives was several times of the active ingredient, CKD-604.