• Anatomy and Cell Biology
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• 제56권1호
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• pp.109-121
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• 2023

Thioacetamide (TAA) exposure and hepatitis C virus infection are usually associated with renal dysfunction. Sofosbuvir (SFV) and daclatasvir (DAC) drugs combination has great value in the treatment of hepatitis C. The study aimed to identify the nephrotoxic effects of TAA and to evaluate the ameliorative role of SFV and DAC in this condition. Forty-eight adult male albino rats were divided into eight groups and received saline (control), SFV, DAC, SFV+DAC, TAA, TAA+SFV, TAA+DAC and TAA+SFV+DAC for eight weeks. Kidney and blood samples were retrieved and processed for histological (Hematoxylin and Eosin and Masson's trichrome), immunohistochemical (α-smooth muscle actin), and biochemical analysis (urea, creatinine, total protein, albumin, malondialdehyde, reduced glutathione, superoxide dismutase, and tumor necrosis factor-α). Examination revealed marked destruction of renal tubules on exposure to TAA with either hypertrophy or atrophy of glomeruli, increase in collagen deposition, and wide expression of α-smooth muscle actin. Also, significant disturbance in kidney functions, oxidative stress markers, and tumor necrosis factor-α. Supplementation with either SFV or DAC produced mild improvement in the tissue and laboratory markers. Moreover, the combination of both drugs greatly refined the pathology induced by TAA at the cellular and laboratory levels. However, there are still significant differences when compared to the control. In conclusion, SFV and DAC combination partially but greatly ameliorated the renal damage induced by TAA which might be enhanced with further supplementations to give new hope for those with nephropathy associated with hepatitis.

• 대한두경부종양학회지
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• 제12권2호
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• pp.230-234
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• 1996

Benign nonepithelial tumors of the thyroid gland are very rare and include lesions such as vascular tumors, smooth muscle tumors and neurogenic tumors. Schwannoma and neurofibroma are benign neoplasms of mesenchymal origin which frequently occur in the head and neck, but their origin within the thyroid gland has rarely been reported. Recently, we encountered two cases of neurogenic tumor of thyroid gland(l schwannoma, 1 neurofibroma) and report them to support the view that Schwannoma and neurofibroma may occur in thyroid gland, which is an unusual site, and are recognizable entities.

• Journal of Chest Surgery
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• 제7권1호
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• pp.117-122
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• 1974

The benign tumor of smooth muscle, the leiomyoma, may arise anywhere in the body, in such tissues as the muscularis of the gut and the media of blood vessels, although by far its most common location is the uterus. Indeed, the mediastinal leiomyomas are extremely rare, especially when one excludes those arising in the esophagus. No dogmatic statements can be made with regard to age, sex distribution, symptoms, or clinical course because of the extreme paucity of cases available for evaluation. Although the majority of the reported cases are in the posterior mediastinum, thus suggesting esophageal origin. We have experienced a right inferior mediastinal leiomyoma in 36 years old housewife. She complained hemoptysis and right lower chest pain associated with intermittent low-graded fever and chillness. Routine X-ray film revealed a large irregular cystic mass density in the right lower thoracic cavity. On exploratory thoracotomy, a huge round yellow-gray colored solid mass, measured about 2.5kg in weight, was located in the right inferior mediastinum just above right hemidiaphragm, medially. The tumor mass was removed, being proved to be leiomyoma on histopathologic examination. Her postoperative course was uneventful and discharged without complication.

• 대한세포병리학회지
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• 제17권2호
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• pp.153-158
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• 2006

Low-grade fibromyxoid sarcoma (LGFMS) is a rare soft tissue tumor. There have been only a few prior fine-needle aspiration (FNA) cytological reports. Recognition of this tumor is important because of its potential for metastasis despite its indolent nature and its deceptively bland cytologic appearance. A 60-year-old male presented with a slowly growing mass in the left calf detected 10 years ago. The patient underwent surgical excision. FNA cytology was performed directly on the mass. The smears showed low cellularity composed of hypercellular tissue fragments, hypocellular loose aggregates, and stripped nuclei. The cytoplasm was seen as either collagenous material or very thin fibrillary collagen strands. Tumor cells had spindle, ovoid, or irregular nuclei, fine chromatin, and small nucleoli. Focally slight degree of nuclear pleomorphism is noted. There were no mitotic figures. Blood vessels were frequently seen. Immunocytochemically, tumor cells were negative for S-100 protein, desmin, smooth muscle actin, and CD34. The diagnosis of LGFMS is rarely possible by cytology alone; however, LGFMS should be included in the differential diagnosis of spindle-cell tumors consisting of hypercellular and hypocellular components with some capillary-sized vessels arising in the deep soft tissue of the lower extremities, particularly the thigh. The immunocytochemical findings are of help in the differential diagnosis.

• 한국임상수의학회지
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• 제23권2호
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• pp.194-196
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• 2006

A leiomyosarcoma that occurred in the right kidney with hepatic metastasis of a 15-year-old intact female Asian water buffalo (Bubalus arnee) in a zoo, Republic of Korea. The animal showed no clinical signs. Grossly, a firm and white mass (6.0 x- 5.0- x 4.0 cm) was observed in the renal capsule and multiple firm white nodules, measuring 1.0-2.5 cm, were present on the surface of the liver. Microscopically, tumor cells were spindle-shaped, the cell density was high, and complex fasciculated tumor cells showing longitudinal and transverse cross-sections were observed. Tumor cell nuclei was ordinarily cigar shaped and had rounded blunt ends. Immunohistochemically, tumor cells reacted intensely with anti-smooth muscle actin antibody. This is the first case report of a renal leiomyosarcoma with hepatic metastasis in a Asian water buffalo.

• 한국임상수의학회지
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• 제29권2호
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• pp.181-185
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• 2012

하부 비뇨기계 폐색을 주증상으로 내원한 11살령의 암컷 시쭈견에서 실시한 초음파 검사에서 뚜렷한 경계를 가지는 저에코의 종괴가 근위 요도의 내강에서 관찰되었다. 조영 검사를 통해 요도의 완전 폐색을 확인하였고, 종양의 범위를 평가하여 방광은 이상 소견이 없으며 종괴가 요도에만 국한하여 발생한 것을 확인하였다. 이를 바탕으로 근위요도에서 유래한 원발성 종양으로 잠정 진단하였다. 수술적으로 종괴와 요도를 함께 제거한 후 요도 문합술을 실시하였다. 이후 임상 증상은 모두 소실되었다. 조직 검사를 통해 종괴는 평활근종으로 진단되었다. 하부 비뇨기계 유래 평활근종은 매우 드물게 보고되어 있으며, 본 보고에서는 영상 진단 과정과 수술적 교정에 대해 소개하였다.

• Biomolecules & Therapeutics
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• 제19권2호
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• pp.187-194
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• 2011

Atherosclerosis and post-angiography restenosis are associated with intimal thickening and concomitant vascular smooth muscle cell (VSMC) proliferation. Obovatol, a major biphenolic component isolated from the Magnolia obovata leaf, is known to have anti-inflammatory and anti-tumor activities. The goal of the present study was to enhance the inhibitory effects of obovatol to improve its potential as a preventive or therapeutic agent in atherosclerosis and restenosis. Platelet-derived growth factor (PDGF)-BB-induced proliferation of rat aortic smooth muscle cells (RASMCs) was examined in the presence or absence of a newly synthesized obovatol derivative, OD78. The observed anti-proliferative effect of OD78 was further investigated by cell counting and [$^3H$]-thymidine incorporation assays. Treatment with 1-4 ${\mu}M$ OD78 dose-dependently inhibited the proliferation and DNA synthesis of 25 ng/ml PDGF-BB-stimulated RASMCs. Accordingly, OD78 blocked PDGF-BB-induced progression from the $G_0/G_1$ to S phase of the cell cycle in synchronized cells. OD78 decreased the expression levels of CDK4, cyclin E, and cyclin D1 proteins, as well as the phosphorylation of retinoblastoma protein and proliferating cell nuclear antigen; however, it did not change the CDK2 expression level. In addition, OD78 inhibited downregulation of the cyclin-dependent kinase inhibitor (CKI) $p27^{kip1}$. However, OD78 did not affect the CKI $p21^{cip1}$ or phosphorylation of early PDGF signaling pathway. These results suggest that OD78 may inhibit PDGF-BB-induced RASMC proliferation by perturbing cell cycle progression, potentially through $p27^{kip1}$ pathway activation. Consequently, OD78 may be developed as a potential anti-proliferative agent for the treatment of atherosclerosis and angioplasty restenosis.

• Preventive Nutrition and Food Science
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• 제15권2호
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• pp.137-142
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• 2010

Previous studies report that organo-sulfur compounds derived from garlic inhibited smooth muscle cell (SMC) proliferation and induced apoptosis of cancer cells. Recently, lipid-soluble compounds such as diallyl sulfides (DAS) and diallyl disulfides (DADS) have been reported to more effectively suppress tumor cell proliferation. However, there were few studies on the suppressive effects of lipid-soluble garlic sulfur compounds on the proliferation and migration of vascular smooth muscle cells (VSMC). Therefore, this study investigated the effect of DAS and DADS on VSMC proliferation/migration induced by oleic acid (OA), a principal fatty acid in circulating triglyceride of blood stream. Assays performed include a tetrazole (MTT) assay, a wound healing assay and a Western blots. VSMC proliferations were enhanced by OA in a dose-dependent manner at concentrations of $10{\sim}50\;{\mu}M$ and inhibited by DAS and DADS compared to non-treated control. OA-induced proliferations were also attenuated by DAS and DADS. OA-induced cell migrations were 2.5 times higher than non-treated control, and they were significantly attenuated by DAS (32% at $150\;{\mu}M$ and 50% at $200\;{\mu}M$) and DADS (40% at $150\;{\mu}M$ and 46% at $200\;{\mu}M$). OA-induced cell migration was also attenuated by PD98059 (ERK inhibitor), SB203580 (P38 inhibitor) and particularly by LY204002 (PI3K inhibitor) and SP600125 (JNK2 inhibitor). Additionally, Western blot assays showed that OA-induced JNK1/2-phosphorylation was down-regulated after treatment with DAS and DADS. In conclusion, the findings of our study support the idea that DAS and DADS may have a suppressive effect on the proliferation and migration of OA-induced VSMC and that this effect may be partly associated with PI3K and JNK2 pathways.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• 제36권6호
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• pp.533-537
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• 2010

Sialadenoma papilliferum (SP) is a rare benign neoplasm that normally arises from the minor salivary glands, particularly in the palate. SP is normally encountered in older men with an exophytic papillary surface growth. In the present study, an SP of the hard palate of a 69-year-old woman was examined immunohistochemically. Myoepithelial cell markers, such as S-100, smooth muscle actin and vimentin, were observed in the basal or luminal layer of tumor cells, indicating that myoepithelial cells participate in the pathogenesis of SP. In addition, cytokeratin 7 was also strongly detected in the tumor cells, suggesting that excretory ductal epithelial cells have a role in its histogenesis. A review of the literature of immunohistochemical studies on SP showed that the expression and co-expression of cytokeratins and myoepithelial cell markers have been reported in tumor cells. These results suggested that excretory duct cells and myoepithelial cells participate in the pathogenesis of SP.

• Journal of Chest Surgery
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• 제31권2호
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• pp.194-197
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• 1998

근상피종은 방추형, 형질세포양, 상피세포양, 투명세포들의 다양한 비율로 구성되는 양성종양으로 기관내 발생빈도가 극히 희귀하여 현재까지 전세계적으로 1례가 보고 되었으며 국내에서는 아직 보고례가 없었다. 본 증례는 우측 경부 종괴를 주소로 내원한 38세 여자 환자로 갑상선 종양및 기관종괴 진단하에 절제문합술을 시행하였다. 절제된 종괴는 주위와 잘 경계지워지는 충실성 조직으로 이루워져 있으며 주로 방추형 혹은 상피양 세포로 구성되고 간간히 세포질의 투명변성이 보였다. 이들 세포는 S-100 단백과 평활근 액틴에 양성이었고 전자현미경 검색상 세포질내에 다량의 소섬유와 기저막 물질이 세포질외에서 관찰되어 양성 근상피종에 합당한 소견을 보였다. 환자는 술후 8개월째 합병증없이 정상생활을 영위하고 있다.