• Title/Summary/Keyword: Small park

Search Result 10,524, Processing Time 0.045 seconds

Induction of Apoptosis by Ethyl Acetate Fraction of Astragalus membranaceus in Human Non-small Cell Lung Cancer Cells - Apoptosis Induction by Astragalus membranaceus -

  • Park, Hyun-Ji;Park, Shin-Hyung
    • Journal of Pharmacopuncture
    • /
    • v.21 no.4
    • /
    • pp.268-276
    • /
    • 2018

  • Objectives: The purpose of this study is to investigate the anti-cancer effects of different fractions of Astragalus membranaceus (AM) in human non-small cell lung cancer (NSCLC) cells. Methods: We isolated hexane, ethyl acetate, and butanol fractions from crude ethanol extract of AM. The cell death was examined by MTT assay and trypan blue exclusion assay. Apoptosis was detected by DAPI staining, annexin V-PI double staining and cell cycle analysis. The expression of apoptosis-related proteins and mitogen-activated protein kinases (MAPKs) was examined by western blot. Results: Among various fractions of AM, the ethyl acetate fraction of AM (EAM) showed the strongest cytotoxic effect in NSCLC cells. EAM reduced the cell proliferation in a time- and dose-dependent manner in NSCLC cells. In addition, EAM induced the chromatin condensation, and increased the population of sub-G1 phase and annexin V-positive cells in a time-dependent manner, indicating that EAM induced apoptosis in NSCLC cells. Consistently, EAM enhanced the expression of cleaved caspase-8 and -9, and induced the accumulation of cleaved- poly (ADP-ribose) polymerase (PARP). Among MAPK proteins, only ERK was dephosphorylated by EAM, suggesting that ERK might be related with EAM-induced apoptosis. Conclusion: Our results clearly demonstrate that EAM exhibited anti-cancer effects in NSCLC cells by induction of apoptosis. We provide a valuable evidence which suggests that AM could be a desirable therapeutic option for treatment of NSCLC.

  • https://doi.org/10.3831/KPI.2018.21.030 인용 PDF KSCI HTML

Variable Stiffness Series Elastic Actuator Design for Active Suspension (능동형 현가장치를 위한 가변 강성 직렬 탄성 액추에이터 설계)

  • Bang, Jinuk;Choi, Minsik;Lee, Donghyung;Park, Jungho;Park, Eunjae;Lee, Geunil;Lee, Jangmyung
    • The Journal of Korea Robotics Society
    • /
    • v.14 no.2
    • /
    • pp.131-138
    • /
    • 2019

  • In this study, we developed an FSEA(Force-sensing Series Elastic Actuator) composed of a spring and an actuator has been developed to compensate for external disturbance forced. The FSEA has a simple structure in which the spring and the actuator are connected in series, and the external force can be easily measured through the displacement of the spring. And the characteristic of the spring absorbs the shock to the small disturbance and increases the sense of stability. It is designed and constructed to control the stiffness of such springs more flexibly according to the situation. The conventional FSEA uses a fixed stiffness spring and the actuator is not compensated properly when it receives large or small external force. Through this experiment, it is confirmed that FSEA compensates the external force through the proposed algorithm that the variable stiffness compensates well for large and small external forces.

  • https://doi.org/10.7746/jkros.2019.14.2.131 인용 PDF KSCI

(S-1,S) Spare-part Inventory Algorithm for Fleet Maintenance : Validation

  • Park Kyung S.
    • Journal of the military operations research society of Korea
    • /
    • v.8 no.2
    • /
    • pp.31-35
    • /
    • 1982

  • Recently, Park [1] proposed an algorithm for the optimum spare-part stock level in an (S-1, S) inventory system for small fleet. This paper reports validative GPSS simulation results and numerical experiences with the algorithm.

  • PDF