• Title/Summary/Keyword: Small animal

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Changes in Total Plate Counts and Quality of Pig Small Intestine by Different Washing and Packaging Methods

  • Kang, Geunho;Seong, Pil-Nam;Ba, Hoa Van;Moon, Sungsil;Cho, Soohyun;Park, Beom-Young;Kang, Sun-Moon;Ham, Hyoung-Joo;Kim, Dayae;Park, Kyoungmi
    • 한국축산식품학회지
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    • 제38권6호
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    • pp.1253-1260
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    • 2018

  • Pig small intestine not only is used as food but also for sausage casings production in many countries worldwide. However, it is well recognized that the small intestine is important source of spoilage and pathogenic bacteria. The present study aimed at investigating the effects of different washing and packaging methods on the changes of microbial levels and physicochemical characteristics of pig small intestine. After collecting and trimming off of visible fats, the pig small intestine samples were treated with; (i) different packaging methods: aerobic packaging (AP), skin packaging (SP), and vacuum packaging (VP); and (ii) washing with different concentrations of acetic acid. The treated samples were then stored at $4^{\circ}C$ for 1, 4, 7, and 10 d. At 1-d storage, higher pH value was found in the AP-treated samples, however, after 7 to 10 days the samples treated with SP had higher values compared to the ones treated with AP and VP (p<0.05). Thiobarbituric acid reactive substances values were higher in the AP-treated samples than those of the SP- and VP- treated samples at 7-d storage (p<0.05). At $10^{th}$ d, total plate counts (TPC) were higher in the control than in the acetic acid-washed samples (p<0.05). Additionally, the TPC was lower in the SP- and VP-treated samples than the AP-treated samples at 7-d storage (p<0.05). These obtained results suggest that the applications of washing with acetic acid solution and/or SP and VP methods could be an effective way to extend the shelf-life of pig small intestine during cold distribution.

  • https://doi.org/10.5851/kosfa.2018.e61 인용 PDF KSCI

Candidate Genes with Ovulation by Differential Display PCR in Small Tail Han Sheep

  • Liu, Shufang;Li, Hongbin;Song, Xuemei;Wang, Aihua;Wei, Caihong;Du, Lixin
    • Asian-Australasian Journal of Animal Sciences
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    • 제19권9호
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    • pp.1229-1233
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    • 2006

  • To find the candidate genes concerned with ovulation rate of sheep, Differential Display Reverse Transcription Polymerase Chain Reaction was employed to find the differently expressed cDNA controlling ovulation in the Small Tail Han sheep of polyembryony and in Tan sheep of single birth. Twenty-four primer pairs of three anchored primers and eight arbitrary primers were assembled to amplify the specialized bands from these sheep. Positive cross tests were applied to optimize the ascertainable PCR conditions in which different special bands can be identified by silver strain in one PCR tube. After eliminating the false positive PCR products by Northern hybridization, 24 differential display bands were acquired from the ovary in the Small Tail Han sheep. These EST bands were sequenced and 18 different ESTs were found in which five ESTs had several copies and 13 ESTs had only one copy. Comparing these ESTs with homologous sequences by BLAST in the GenBank, there were six ESTs with known open reading frame (ORF) and function, three ESTs with known ORF and no function, and 9 ESTs without homologous sequence. These ESTs partly represent several genes such as NOS2, tensin, TCRA, CDKN1A, ESR1 and ACTB which express especially in Small Tail Han sheep.

  • https://doi.org/10.5713/ajas.2006.1229 인용 PDF KSCI