• Journal of the Korean Society for information Management
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• v.36 no.1
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• pp.169-190
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• 2019

This study aims to identify the effects of teachers' recognition and application of 2015 revised national curriculum on their educational information needs in high schools. Several in-depth interviews and a questionnaire survey with the teachers, who were in charge of teaching common courses, such as Korean language, mathematics, English, social studies, and science, in general public high schools in Incheon, were executed for the purpose. As a result, the teachers' recognition and application affected their educational information needs in part. Especially, new demands on small sized copies and learning information sources were identified which were related to the application of 2015 revised national curriculum. Based on the results, we proposed several improvements of school library operations, such as small sized local consortium for sharing resources and providing referral services, in order to strengthen the gateway role of school libraries.

• Journal of the Korean Institute of Educational Facilities
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• v.13 no.2
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• pp.42-49
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• 2006

We have analysed the use and organization of space in the Centres des Documentations et de Informations(CDI) in lycees in France with the equivalent information centres. The majority of CDI are located in close proximity to the Academic staff and provide easy student access. The CDI provide not only books but also reviews, magazines and CD-Rom. This information is very important to pupils both in the pursuit of their higher studies and also with their future professional life as there is also careers information available to be discussed regularly with the careers advisor. The function of the CDI is not only to provide information. As part of the curriculum there are classes on how to access the information available in the CDI as well as Seminars and Audio-Visual courses. This management makes it possible for the CDI to operate, not only as a school library but also as a multi-function centre of documentation and information; a variety of spaces are also available: a room for private/small group study. A computer room (as well as a research corner), a reading room, monthly review room, photocopy room, rest room, exhibition room, careers information room, audio visual room etc. The results of this study can be used as essential information during the space planning of Korean school libraries in the future.

• Journal of Microbiology and Biotechnology
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• v.30 no.7
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• pp.1092-1096
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• 2020

YjiC, a glycosyltransferase from Bacillus licheniformis, is a well-known versatile enzyme for glycosylation of diverse substrates. Although a number of O-glycosylated products have been produced using YjiC, no report has been updated for nucleophilic N-, S-, and C- glycosylation. Here, we report the additional functional capacity of YjiC for nucleophilic N- and S- glycosylation using a broad substrate spectrum including UDP-α-D-glucose, UDP-N-acetyl glucosamine, UDP-N-acetylgalactosamine, UDP-α-D-glucuronic acid, TDP-α-L-rhamnose, TDP-α-D-viosamine, and GDP-α-L-fucose as donor and various amine and thiol groups containing natural products as acceptor substrates. The results revealed YjiC as a promiscuous enzyme for conjugating diverse sugars at amine and thiol functional groups of small molecules applicable for generating glycofunctionalized chemical diversity libraries. The glycosylated products were analyzed using HPLC and LC/MS and compared with previous reports.

• Journal of Plant Biotechnology
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• v.7 no.1
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• pp.51-55
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• 2005

Many cloning vectors in which cDNAs can be inserted to the sense orientation have been developed. Uni-ZAP XR vector (Stratagene) should contain clones that are oriented to sense direction with respect to T3 RNA polymerase primer. Unexpectedly, large portions of cDNAs in Chinese cabbage cDNA library showed unusual insertions, antisense orientation and a hybrid of two different clones. Using two clones, 4H03 and 53-B10, derived from different cDNA libraries, we proposed and demonstrated the possibility of unusual-construct formation by in vitro translation and northern blot analysis. The 4H03 clone was inserted with inverse direction, and its transcript and translation product could be produced by T7 RNA polymerase, indicating that this clone is definitely inserted into inverse orientation. The 53-B10 that contains two independent genes was turned out to be a hybrid in which two genes are inserted to opposite direction each other. All unusual constructs might be due to the presence of small fragments of DNA, like adapter. However, the mechanism underlined the formation of unusual constructs is still remain to be solved.

• The Bulletin of The Korean Astronomical Society
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• v.42 no.1
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• pp.57.2-57.2
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• 2017

Remote and robotic telescopes are the most effective instrument for astronomical survey projects. The system is based on the dynamic operation of all astronomical instruments such as dome and telescope control system (TCS), focuser, filter wheel and data taking camera. We adopt the ASCOM driver platform to control the instruments through the integrated software. It can convert different interface libraries from various manufacturers into a uniform standard library. This allows us to effectively control astronomical instruments without modifying codes. We suggest a conceptual design of software for automation of a small telescope such as the new wide-field 0.25m telescope at McDonald Observatory. It can also be applied to operation of multi-telescopes in future projects.

• Korean Journal of Microbiology
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• v.40 no.4
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• pp.359-363
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• 2004

I investigated an effective way to generate a metagenomic library from DNA prepared from environental samples. The sizes of DNA extracted from environmental samples were usually in the range of 10 to 100 kbp as estimated from $0.4\%$ agarose gel electrophoresis. Because of this small size, a fosmid, rather than BAC, was chosen as a vector. It was found that, for the successful generation of metagenomic library, the selection of DNA with the sized of about 40 kbp was critical and, therefore, a simple agarose gel electrophoresis system was developed to select this size of DNA. By the procedure described in this report, I obtained metagenomic libraries containing 25,000 fosmid clones, which corresponded to 1,000 Mb of metagenomic DNA.

• Journal of the Korean Society for Library and Information Science
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• v.14
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• pp.93-130
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• 1987

Scientific communication is an information exchange activity between scientists. Scientific communication is carried out in a variety of informal and formal ways. Basically, informal communication takes place by word of mouth, whereas formal communication occurs via the written word. Science is a highly interdependent activity in which each scientist builds upon the work of colleagues past and present. Consequently, science depends heavily on scientific communication. In this study, three mathematical models, namly Brillouin measure, logistic equation, and Markov chain are examined. These models provide one with a means of describing and predicting the behavior of scientific communication process. These mathematical models can be applied to construct quality filtering algorithms for subject literature which identify synthesized elements (authors, papers, and journals). Each suggests a different type of application. Quality filtering for authors can be useful to funding agencies in terms of identifying individuals doing the best work in a given area or subarea. Quality filtering with respect to papers can be useful in constructing information retrieval and dissemination systems for the community of scientists interested m the field. The quality filtering of journals can be a basis for the establishment of small quality libraries based on local interests in a variety of situations, ranging from the collection of an individual scientist or physician to research centers to developing countries. The objective of this study is to establish the theoretical framework for informetrics which is defined as the quantitative analysis of scientific communication, by investigating mathematical models of scientific communication.

• 한국전산유체공학회:학술대회논문집
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• 2002.05a
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• pp.8-15
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• 2002

For the large scale computation of turbulent flows around an arbitrarily shaped body, a parallel LES (large eddy simulation) code has been recently developed in which domain decomposition method is adopted. METIS and MPI (message Passing interface) libraries are used for domain partitioning and data communication between processors, respectively. For unsteady computation of the incompressible Wavier-Stokes equation, 4-step splitting finite element algorithm [1] is adopted and Smagorinsky or dynamic LES model can be chosen fur the modeling of small eddies in turbulent flows. For the validation and performance-estimation of the parallel code, a three-dimensional laminar flow generated by natural convection inside a cube has been solved. Then, we have solved the turbulent flow around MIRA (Motor Industry Research Association) model at $Re = 2.6\times10^6$, which is based on the model height and inlet free stream velocity, using 32 processors on IBM SMP cluster and compared with the existing experiment.

• Asian-Australasian Journal of Animal Sciences
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• v.14 no.6
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• pp.880-884
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• 2001

Rumen microbiology research has undergone several evolutionary steps: the isolation and nutritional characterization of readily cultivated microbes; followed by the cloning and sequence analysis of individual genes relevant to key digestive processes; through to the use of small subunit ribosomal RNA (SSU rRNA) sequences for a cultivation-independent examination of microbial diversity. Our knowledge of rumen microbiology has expanded as a result, but the translation of this information into productive alterations of ruminal function has been rather limited. For instance, the cloning and characterization of cellulase genes in Escherichia coli has yielded some valuable information about this complex enzyme system in ruminal bacteria. SSU rRNA analyses have also confirmed that a considerable amount of the microbial diversity in the rumen is not represented in existing culture collections. However, we still have little idea of whether the key, and potentially rate-limiting, gene products and (or) microbial interactions have been identified. Technologies allowing high throughput nucleotide and protein sequence analysis have led to the emergence of two new fields of investigation, genomics and proteomics. Both disciplines can be further subdivided into functional and comparative lines of investigation. The massive accumulation of microbial DNA and protein sequence data, including complete genome sequences, is revolutionizing the way we examine microbial physiology and diversity. We describe here some examples of our use of genomics- and proteomics-based methods, to analyze the cellulase system of Ruminococcus flavefaciens FD-1 and explore the genome of Ruminococcus albus 8. At Illinois, we are using bacterial artificial chromosome (BAC) vectors to create libraries containing large (>75 kbases), contiguous segments of DNA from R. flavefaciens FD-1. Considering that every bacterium is not a candidate for whole genome sequencing, BAC libraries offer an attractive, alternative method to perform physical and functional analyses of a bacterium's genome. Our first plan is to use these BAC clones to determine whether or not cellulases and accessory genes in R. flavefaciens exist in clusters of orthologous genes (COGs). Proteomics is also being used to complement the BAC library/DNA sequencing approach. Proteins differentially expressed in response to carbon source are being identified by 2-D SDS-PAGE, followed by in-gel-digests and peptide mass mapping by MALDI-TOF Mass Spectrometry, as well as peptide sequencing by Edman degradation. At Ohio State, we have used a combination of functional proteomics, mutational analysis and differential display RT-PCR to obtain evidence suggesting that in addition to a cellulosome-like mechanism, R. albus 8 possesses other mechanisms for adhesion to plant surfaces. Genome walking on either side of these differentially expressed transcripts has also resulted in two interesting observations: i) a relatively large number of genes with no matches in the current databases and; ii) the identification of genes with a high level of sequence identity to those identified, until now, in the archaebacteria. Genomics and proteomics will also accelerate our understanding of microbial interactions, and allow a greater degree of in situ analyses in the future. The challenge is to utilize genomics and proteomics to improve our fundamental understanding of microbial physiology, diversity and ecology, and overcome constraints to ruminal function.

• Journal of Life Science
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• v.17 no.8 s.88
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• pp.1082-1089
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• 2007

Serological anlysis of recombinant cDNA expression libraries (SEREX) has led to identification of several categories of new antigens recognized by the immune system of cancer patients, which are referred to as the cancer immunome. We analyzed normal testis cDNA expression libraries with serumobtained from non-small lung cancer patient and isolated 40 distinct antigen designated KP-LuT-1 through KP-LuT-40. Among these antigens 20 antigens were previously identified by SEREX analysis of other tumor types, and 20 out of 40 antigens (50%) did not match entries in Cancer Immunome Database and were considered newly identified antigens. Sequencing analysis showed that the anti-gens comprised 26 functional known proteins and 14 noble/uncharacterized gene products. Of these, the hypothetical protein KP-LuT-6 was shown tissue-restricted. RT-PCR showed it to be expressed strongly only in normal testis. In addition to normal tissues-restricted expression, KP-LuT-6 mRNA was detected in lung tumor samples(3/l0), stomach tumor samples(3/l0), and breast tumor samples(l/5), whereas not detected in colon tumor samples(O/I2). These data suggest that KP-LuT-6 is a cancer/testis (CT)-like antigen as a potential target for cancer immunotherapies.