• 한국지하수토양환경학회:학술대회논문집
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• 한국지하수토양환경학회 2001년도 추계학술발표회
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• pp.184-187
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• 2001

This study presents procedure and analysis method for not well known slug interference tests. Results of the slug interference tests were compared with those of pumping and recovery tests and conventional slug tests.

• Asian Pacific Journal of Cancer Prevention
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• 제13권10호
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• pp.4947-4951
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• 2012

Objective: Lung cancer is a deadly cancer, whose kills more people worldwide than any other malignancy. SLUG (SNAI2, Snail2) is involved in the epithelial mesenchymal transition in physiological and in pathological contexts and is implicated in the development and progression of lung cancer. Methods: We constructed a lentivirus vector with SLUG shRNA (LV-shSLUG). LV-shSLUG and a control lentivirus were infected into the non-small cell lung cancer cell A549 and real-time PCR, Western blot and IHC were applied to assess expression of the SLUG gene. Cell proliferation and migration were detected using MTT and clony formation methods. Results: Real-time PCR, Western Blot and IHC results confirmed down-regulation of SLUG expression by its shRNA by about 80%~90% at both the mRNA and protein levels. Knockdown of SLUG significantly suppressed lung cancer cell proliferation. Furthermore, knockdown of SLUG significantly inhibited lung cancer cell invasion and metastasis. Finally, knockdown of SLUG induced the down-regulation of Bcl-2 and up-regulation of E-cadherin. Conclusion: These results indicate that SLUG is a newly identified gene associated with lung cancer growth and metastasis. SLUG may serve as a new therapeutic target for the treatment of lung cancer in the future.

• 치위생과학회지
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• 제17권5호
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• pp.433-438
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• 2017

Relative to its incidence, oral cancer has serious negative social effects. The exact causes of oral cancer have not been clarified, but many studies have implicated smoking and drinking. However, the fundamental mechanism of oral cancer causation has yet to be elucidated. Lysophosphatidic acid (LPA) augments epithelial mesenchymal transition (EMT) and development of various cancer cells. However, a detailed mechanistic explanation for LPA-induced EMT and the effects of EMT-promoting conditions on oral squamous cell carcinoma development remain elusive. In the present study, a quantitative reverse transcription polymerase chain reaction was used to analyze TWIST1, Slug, E-cadherin, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) transcript expression. Immunoblotting was used to analyze TWIST1, Slug, E-cadherin, and GAPDH protein expression. siRNAs were used to silence TWIST1 and Slug transcript expression. A matrigel-coated in vitro invasion insert was used to analyze oral cancer cell invasion. The results of the present study show that the expression levels of TWIST1 and Slug, which are EMT factors, were increased by LPA treatment in YD-10B oral squamous cell carcinoma. Conversely, E-cadherin expression was significantly reduced. In addition, transfection of the cells with TWIST1 and Slug siRNA strongly inhibited LPA-induced oral cancer cell invasion. The present study shows that TWIST1 and Slug mediate LPA-induced oral cancer cell EMT and invasiveness. The present study confirmed the mechanism by which LPA promotes oral cancer cell development, with TWIST1 and Slug providing novel biomarkers and promising therapeutic targets for oral cancer cell development.

• 소성∙가공
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• 제24권6호
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• pp.425-430
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• 2015

In the current paper, the effects of initial slug design on the earring of an Al rectangular battery case manufactured by impact extrusion were studied. During impact extrusion, non-uniform metal flow between the long and the short sides of the battery case leads to earring, which is subsequently trimmed. Process parameters such as friction, aspect ratio of the battery case, the die shape and the forming temperature tend to induce earring because they cause greater non-uniform metal flow. Large aspect ratio of the battery case and high friction between slug and die can greatly affect the earring of a rectangular battery case. To make a rectangular battery case without earring, it is necessary to control metal flow uniformly during impact extrusion. One of the ways to reduce the earring is to control the metal flow of slug at the initial upsetting stage. To analyze the effects of the initial slug design on earring, FE analysis was conducted using DEFORM 3D. Two types of initial slug designs were evaluated where volume was removed along either the width or thickness directions. The results show that the initial slug design can be effective in adjusting the uniformity of metal flow.

• 대한기계학회논문집B
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• 제28권12호
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• pp.1521-1527
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• 2004

Thermocapillary migration of a liquid slug is caused by temperature difference between the ends of a slug. The temperature difference induces the difference of the surface tension coefficient and consequently of capillary pressure between the ends of the slug. Presently available model to predict a velocity of thermocapillary migration adopts the Poiseuille equation which is valid only for a very long slug and neglects the shear stress near the contact line. In the present study, a new model has been developed to consider the shear stress near the contact line so that it can be applied to slugs or drops of general configuration. The experiments using mineral oil with the length to diameter ratio being 10 and a glass capillary were performed. It was found that the liquid slug began to move upon overcoming contact angle hysteresis when the temperature difference reached 35$^{\circ}C$. The results indicate that the new model well predicts the velocity of the liquid slug.

• Proceedings of the National Institute of Ecology of the Republic of Korea
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• 제5권3호
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• pp.86-93
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• 2024

Unintentional dispersal of organisms has explosively increased due to expansion of human activities. Among introduced organisms, some organisms are categorized as invasive species because of their effects on environmental risk, economic loss, and human health. In 2023, a leopard slug (Limax maximus) was reported in Suwon, Republic of Korea. This slug was designated as a potential invasive species because a wide range of plant species were identified as food sources for this slug in its original habitats. However, it is difficult to investigate the ecological risk of this newly introduced slug in Republic of Korea. Therefore, the potential ecological risk from this newly introduced slug was estimated by meta-genome analyses of its feces. Through meta-genome analyses, 22 Families, 28 Genera, and 26 Species of land plants were identified. Among these 26 identified plant species, six economically important crops - squash (Cucurbita maxima), tomato (Solanum lycopersicum), potato (Solanum tuberosum), cowpea (Vigna unguiculata), rice (Oryza sativa), and oriental melon (Cucumis melo) - were identified. Therefore, leopard slugs potentially could cause economic losses in Republic of Korea. Further study is required to build a control strategy against leopard slugs.

• 대한지하수환경학회지
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• 제7권2호
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• pp.89-96
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• 2000

Slug시험은 수리전도도 예측에 가장 널리 쓰이는 편리한 대수층 실험법이다. Slug시험 중인 관측정 수위 변동은 관측정 반경, 스크린 길이, 다공 매질의 수리전도도와 비저유계수에 영향을 받는다. 이 연구에서는 유한요소기법을 이용한 새 slug시험 모델을 개발하고 그 유용성을 시험하였다. 모델에서 관측정의 수위변동과 다공매질내 지하수유동을 반복기법(iteration technique)을 적용하여, 스크린상의 유동량 측정으로 연결하였다. 이 모델의 수치적 정확도는 Cooper et al. (1967)의 분석해에 대해 검증되었다, 본 방법은 주변 모니터링이 가능한 slug시험의 시뮬레이션, 부분관통과 비저유 계수의 반영 등의 장점이 있다. Slug시험을 통한 다공매질 반영 범위는 비저유계수에 민감하다. 작은 비저유계수일수록 수두압 변화 전파범위가 커져 그 반영률이 증가한다. 관측정의 표준곡선 비교를 통해 비저유계수 예측이 어려우므로, 다공매질 내에서 관측된 수두변화의 표준곡선 활용이 유용할 것이다. 지하수유동의 수직 성분이 커질수록 관측정 수위 회복에 대한 비저유계수의 영향은 더 감소한다. 실제 관측정 주변의 수평-수직한 지하수 유동 해석시, 비저유계수의 무시와 관측데이터의 적용구간 선택에 의한 수리전도도 예측 편차는 거의 무시할 만하며, 수평유동의 경우 분석방법상 편차가 약간 발생한다.

• 한국지반공학회:학술대회논문집
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• 한국지반공학회 2008년도 춘계 학술발표회 초청강연 및 논문집
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• pp.220-228
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• 2008

In constructing a slurry trench cutoff wall, a thin but relatively impermeable layer called filter cake can be formed on the excavation surface. The filter cake significantly influences the result of slug test analysis in the cutoff wall. This study is to examine the effect of filter cake on evaluating in situ hydraulic conductivity of the vertical cutoff wall along with slug test analyses. The numerical program Slug_3D was modified to take filter cake into account in the slug test simulation. With consideration of filter cake, the type curve method and the modified line-fitting method were used to reanalyze the case study taken from a landfill site. The previous results achieved by Choi and Daniel (2006b) without consideration of filter cake have been compared with the results in this study. The considerable difference between the two results shows the necessity of considering the filter cake in practice.

• 대한기계학회논문집B
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• 제29권6호
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• pp.671-686
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• 2005

The transient nature and complex geometries of two-phase gas-liquid flows cause fundamental difficulties when measuring flow velocity using an electromagnetic flowmeter. Recently, a current-sensing flowmeter was introduced to obtain measurements with high temporal resolution (Ahn et al.). In this study, current-sensing flowmeter theory was applied to measure the fast velocity transients in slug flows. The velocity fields of axisymmetric gas-liquid slug flow in a vertical pipe were obtained using Volume-of-Fluid (VOF) method, and the virtual potential distributions for the electrodes of finite size were also computed using the finite volume method for simulating slug flow. The output signal prediction for slug flow was carried out from the velocity and virtual potential (or weight function) fields. The flowmeter was numerically calibrated to obtain the cross-sectional liquid mean velocity at an electrode plane from the predicted output signal. Two calibration parameters are proposed for this procedure: a flow pattern coefficient and a localization parameter. The flow pattern coefficient was defined by the ratio of the liquid resistance between the electrodes for two-phase flow with respect to that for single-phase flow, and the localization parameter was introduced to avoid errors in the flowmeter readings caused by liquid acceleration or deceleration around the electrodes. These parameters were also calculated from the computed velocity and virtual potential fields. The results can be used to obtain the liquid mean velocity from the slug flow signal measured by a current-sensing flowmeter.

• 대한기계학회:학술대회논문집
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• 대한기계학회 2004년도 춘계학술대회
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• pp.1951-1956
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• 2004

The transient nature and complex flow geometries of two-phase gas-liquid flows cause fundamental difficulties when measuring flow velocity using an electromagnetic flowmeter. Recently, a current-sensing flowmeter was introduced to obtain measurements with high temporal resolution (Ahn et $al.^{(1)}$). In this study, current-sensing flowmeter theory was applied to measure the fast velocity transients in slug flows. To do this, the velocity fields of axisymmetric gas-liquid slug flow in a vertical pipe were obtained using Volume-of-Fluid (VOF) method and the virtual potential distributions for the electrodes of finite size were also computed using the finite volume method for the simulated slug flow. The output signal prediction for slug flow was carried out from the velocity and virtual potential (or weight function) fields. The flowmeter was numerically calibrated to obtain the cross-sectional liquid mean velocity at an electrode plane from the predicted output signal. Two calibration parameters are required for this procedure: a flow pattern coefficient and a localization parameter. The flow pattern coefficient was defined by the ratio of the liquid resistance between the electrodes for two-phase flow with respect to that for single-phase flow, and the localization parameter was introduced to avoid errors in the flowmeter readings caused by liquid acceleration or deceleration around the electrodes. These parameters were also calculated from the computed velocity and virtual potential fields. The results can be used to obtain the liquid mean velocity from the slug flow signal measured by a current-sensing flowmeter.