• 대한약리학회:학술대회논문집
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• 대한약리학회 2006년도 The 6th Congress of the Federation of Asian and Oceanian Physiological Societies
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• pp.85.1-85.1
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• 2006

• Clinical and Experimental Reproductive Medicine
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• 제27권3호
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• pp.283-289
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• 2000

Objective: The study was performed to compare the survival rate and the development of day 2 mouse embryos which had freezing procedures done. Methods: We used three different vitrification solutions (EFS, VS14, DPS) and a ultrarapid freezing solution (UFS) for cryopreservation of day 2 mouse embryo. Results: We tested toxicity by exposing embryos to vitirification solutions and a ultrarapid freezing solution. The survival rates are 100%, 97.8%, 95.6% and 100% (EFS, VS14, DPS and UFS). After cultured for 96 hours, hatching rates of each group are 93.5% (no freezing), 95.6% (EFS), 86.4% (VS14), 93.0% (DPS), and 93.0% (UFS). There is no significant differences among groups. The survival rates after thawing cryopreserved embryos are 80.2%, 91.7%, 69.5%, 0% and 91.8% (slow freezing, EFS, VS14, DPS and UFS). Also cultured for 96 hours, the hatching rates are 93.5% (no freezing), 84.1% (slow freezing), 93.9% (EFS), 48.5% (VS14) and 70.1% (UFS). Conclusion: The survival rates of vitrification in EFS solution and ultrarapid freezing are higher than slow freezing (p<0.05). The hatching rate of vitrification in EFS solution cultured for 96 hours is highest, so vitrification of day 2 mouse embryos in EFS solution considered as more effective for cryopreservation.

• 대한생식의학회:학술대회논문집
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• 대한불임학회 2005년도 제48차 춘계 학술대회
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• pp.126-126
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• 2005

• 사상체질의학회지
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• 제19권3호
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• pp.126-143
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• 2007

1. Objectives The purpose of this study is to find out the grade of practical use, the important element and the significant characteristics of Sasangin' s face, voice, skin and pulse diagnostic impression, in Sasang constitutional clinical diagnosis. 2. Methods We analysed the survey data about Sasangin' s face, voice, skin and pulse diagnostic impression, drawn up by specialist in Sasang constitutional medicine. 3. Results and Conclusions (1) In the application degree of face feature, the case which it was applied with 20-40% and 40-60% were 16 people(43.2%) respectively. In voice, the case applied with 0-20% was 19 people(51.4%), in skin, 0-20% and 20-40% were 14 people(37.8%) respectively and in pulse diagnosis, 0-20% were 25 people(73.0%). (2) In constitutional diagnosis, the important element of face were 'frontal whole shape', 'whole impression' and 'size and shape of eye, ear, mose and mouth', the important element of voice 'speed of speech', 'purity and impurity' and 'pitch', the important element of skin 'thickness', 'feel of touch' and 'size of skin pores' and the important element of pulse diagnosis 'speed of pulse', 'sinking and floating' and 'weakness and firmness'. (3) The important face characteristics of Taeyangin were 'bright eye', 'broad forehead' and 'strong impression', Soyangin 'protruding forehead', 'thin and small lips', 'narrowing and sharp chin', Taeumin 'thick lips', 'flat face', 'large eye, nose, ear and mouth' and Soeumin 'long and slender face', 'downward slanting eyes' and 'small eye, nose, ear and mouth', The important voice characteristics of Taeyangin were 'loud' and 'clear', Soyangin 'rapid' and 'high-pitched tone', Taeumin 'chick', 'slow' and 'low-pitched tone' and Soeumin 'small and feeble' and 'slow'. The important skin characteristics of Taeyangin were 'thin' and 'white', Soyangin 'thin', 'smooth' and 'elastic', Taeumin 'thick', 'large skin-pore', 'coarse' and Soeumin 'soft', 'thin' and 'subtle skin-pore'. The important pulse characteristics of Taeyangin were 'rapid' and 'large', Soyangin 'rapid' and 'floating', Taeumin 'tense', 'long' and 'solid' and Soeumin 'fine', 'weak' and 'slow'.

• The Korean Journal of Physiology and Pharmacology
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• 제17권6호
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• pp.537-546
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• 2013

Deiters' cells are the supporting cells in organ of Corti and are suggested to play an important role in biochemical and mechanical modulation of outer hair cells. We successfully isolated functionally different $K^+$ currents from Deiters' cells of guinea pig using whole cell patch clamp technique. With high $K^+$ pipette solution, depolarizing step pulses activated strongly outward rectifying currents which were dose-dependently blocked by clofilium, a class III anti-arrhythmic $K^+$ channel blocker. The remaining outward current was transient in time course whereas the clofilium-sensitive outward current showed slow inactivation and delayed rectification. Addition of 5 mM tetraethylammonium (TEA) further blocked the remaining current leaving a very fast inactivating transient outward current. Therefore, at least three different types of $K^+$ current were identified in Deiters' cells, such as fast activating and fast inactivating current, fast activating slow inactivating current, and very fast inactivating transient outward current. Physiological role of them needs to be established.

• Journal of Acupuncture Research
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• 제29권5호
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• pp.151-158
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• 2012

Objectives : We hypothesized that reinforcing-reducing manipulations produce different effects by quantity of stimulation, and examined whether the difference is statistically significant or not. Methods : The study was conducted to the students of College of Oriental Medicine, Dongguk University. We needled into $ST_{36}$ and applied 6 kinds of basic reinforcing-reducing manipulations in Huang di nei jing by randomized sequence. And we had the students to fill up the questionnaire in form of visual analog scale(VAS). We examined whether significant differences in VAS existed between reinforcing-reducing manipulations(by t-test). And we examined whether significant differences in VDRRT(VAS difference between reinforcing-reducing technique) values existed along the gender, body mass index(BMI), age(by t-test), and psychological sensitivity(by ANOVA). Results : 1. The total stimulation quantity from reducing technique was significantly higher than that from reinforcing technique. Individually VAS values of reducing techniques were significantly higher in rotation, slow-rapid, lift-thrust, respiration and open-close manipulation. 2. The highest stimulation quantity was from rotation manipulation. And the highest VDRRT value was from slow-rapid manipulation. 3. When VDRRT values were analyzed by gender, age, BMI, and psychological sensitivity, VDRRT was significantly different by BMI in rotation manipulation, and by psychological sensitivity in rotation, lift-thrust, and slow-rapid manipulation. Conclusions : We could verify that reinforcing-reducing techniques induce different therapeutic effects by different quantities of stimulations. It suggests that reinforcing-reducing techniques could be applied universally, regardless of gender or age. Further studies are needed to consider control group, or patient groups in characteristic conditions.

• The Korean Journal of Physiology and Pharmacology
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• 제14권5호
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• pp.291-297
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• 2010

The sleep homeostatic response significantly affects the state of anesthesia. In addition, sleep recovery may occur during anesthesia, either via a natural sleep-like process to occur or via a direct restorative effect. Little is known about the effects of isoflurane anesthesia on sleep homeostasis. We investigated whether 1) isoflurane anesthesia could provide a sleep-like process, and 2) the depth of anesthesia could differently affect the post-anesthesia sleep response. Nine rats were treated for 2 hours with $ad$ $libitum$ sleep (Control), sleep deprivation (SD), and isoflurane anesthesia with delta-wave- predominant state (ISO-1) or burst suppression pattern-predominant state (ISO-2) with at least a 1-week interval. Electroencephalogram and electromyogram were recorded and sleep-wake architecture was evaluated for 4 hours after each treatment. In the post-treatment period, the duration of transition to slow-wave-sleep decreased but slow wave sleep (SWS) increased in the SD group, but no sleep stages were significantly changed in ISO-1 and ISO-2 groups compared to Control. Different levels of anesthesia did not significantly affect the post-anesthesia sleep responses, but the deep level of anesthesia significantly delayed the latency to sleep compared to Control. The present results indicate that a natural sleep-like process likely occurs during isoflurane anesthesia and that the post-anesthesia sleep response occurs irrespective to the level of anesthesia.

• Journal of Preventive Medicine and Public Health
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• 제31권2호
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• pp.275-284
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• 1998

1996년 3월부터 1996년 12월까지 충북대학교병원 비뇨기과에 입원하여 치료를 받은 방광암 환자 67명과 암 아닌 다른 질환을 가진 대조군 67명을 대상으로 흡연, 음주, 직업력 등을 포함한 생활 습관과 NAT2와 GSTM1, 그리고 GSTT1 유전자 다형성 양상을 조사하여 다음과 같은 결론을 얻었다. 1. NAT2 다형성 분포는, 환자군이 slow, intermediate, rapid acetylator가 각각 3.0%, 38.8%, 58.2%, 그리고 대조군이 7.6%, 40.9%, 51.5%였으며, NAT2의 활성과 방광암 위험도 사이의 관련성은 유의하지 않았다($\chi^2_{trend}=1.18$, P-value>0.05). 2. GSTM1 결손은 환자군의 68.7%, 대조군의 49.3%에서 확인되었으며, OR(95% 신뢰구간)이 2.23(1.12-4.56)으로, 방광암 발생의 위험인자로 나타났다. 3. GSTT1은 환자군의 26.9%,그리고 대조군의 43.3%에서 결손이 있는 것으로 나타나서, GSTT1 결손은 방광암에 대하여 보호효과가 있는 것으로 관찰되었다(OR: 0.48, 95% 신뢰구간: 0.23-0.99). 4. 흡연 여부는 방광암의 발생에 유의한 영향을 미치지 않는 것으로 나타났는데(OR=1.85, 95% CI: 0.85-4.03), 이는 환자군과 대조군의 흡연률이 모두 높기 때문으로 판단된다. 5. 그 외, 음주력, 직업력, 수혈 여부, 그리고 피임시술의 과거력 등의 요인들은 방광암 발생과 유의한 관련성이 없는 것으로 나타났다.

• 대한한방내과학회지
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• 제28권4호
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• pp.727-732
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• 2007

Objectives : This study investigated using National Institutes of Health Strokes Scale in acute and subacute stroke patients and evaluated relativity of pulse pattern to NIHSS. Methods : 104 acute and subacute stroke patients were selected from 4 oriental medical hospitals from April 2007 to May 2007. The patients were admitted to hospital within 1 month after stroke. Pulse feeling diagnosis was done by oriental medical doctor and classified into 7 subtypes. Results : There were significant results statistically between slow/rapid pulse and NIHSS score in the male group; in the female group, there were no significant results statistically but the results showed that slow pulse has lower NIHSS score than rapid pulse. Patients with surging pulse had lower NIHSS score than the other patients. Another pulse pattern had no relativity to NIHSS score.

• 대한한방내과학회지
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• 제44권1호
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• pp.1-11
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• 2023

Objective: There are currently no disease-modifying medications or definite long-term sustainable interventions for patients with Parkinson's disease (PD), indicating an unmet treatment need. Our goal was to create a long-term sustainable intervention for PD patients that can be used in Korean medicine clinics. Methods: The Meridian Activation Remedy System (MARS) was created to stimulate a patient's 12 meridians and sinew channels using a combination of acupoint stimulation and exercise. The acupoints and motions used in MARS were selected through literature studies and expert advice. The methodologies were refined using observational and case studies. With slow and fast movements, the MARS intervention was intended to activate both slow- and fast-twitch muscle fibers. Intradermal acupuncture and motion that shift the center of gravity were employed to enhance the patient's balance and proprioception. In addition, the intervention included alternating movement exercises to address the complex cognitive decline commonly occurring in PD patients. Results: The following acupoints were chosen for the MARS intervention: bilateral Hegu (LI4), Houxi (SI3), Waiguan (TE5), Neiguan (PC6), Zhongchong (PC9), Yuji (LU10), Zusanli (ST36), Yanglingquan (GB34), Taichong (LR3), Kunlun (BL60), and Taixi (KI3). We also developed actions that can stimulate the body's 12 meridians. Conclusion: We developed the MARS intervention, which combines acupuncture and exercise, to address the unmet therapeutic needs of PD patients. We hope that with additional research, the MARS intervention can be set as an effective therapeutic program for PD patients.