• The Journal of Korean Medical History
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• v.26 no.2
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• pp.63-74
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• 2013

Objective : In Donguibogam(東醫寶鑑), like a slow pulse with irregular intervals(結脈), an abrupt pulse(促脈) and a slow and weak pulse with regular intervals(代脈), an uneven pulse is written as a kind of arrhythmia. Such a recognition differs from the present one. In this study, I try to seize the meaning and deliberate whether those expressions are appropriate. Method : 1. Collect and compare the materials of the above four pulses among documentary records of feeling the pulse for diagnosis. 2. Grasp the aspect and trend of changes. 3. Reason whether records in Donguibogam(東醫寶鑑) are proper or not. Result & Conclusions : Donguibogam(東醫寶鑑) written "like a slow pulse with irregular intervals(結脈), an abrupt pulse(促脈) and a slow and weak pulse with regular intervals(代脈), an uneven pulse is written as a kind of arrhythmia". It is not 'an uneven pulse' is written about irregularity of pulse, but 'an uneven pulse' is written about irregularity of the sense able to be felt on the fingertip.

• Journal of Ginseng Research
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• v.35 no.3
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• pp.325-330
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• 2011

Red ginseng saponin fraction-A (RGSF-A) contains a high percentage of panaxadiol saponins that were isolated from Korean red ginseng by ultrafiltration. The aim of this study was to elucidate the effects of RGSF-A on the porcine distal left anterior descending (LAD) coronary artery. The relaxant responses to RGSF-A were examined during contractions induced by 100 nM U46619 (9,11-dideoxy-9a,11a-methanoepoxy-prostaglandin F2a), a stable analogue of thromboxane A2. RGSF-A dose-dependently induced biphasic (fast- and slow-) relaxation in the distal LAD coronary artery in the presence of an intact endothelium. The fast-relaxation was quickly achieved in a minute, and then the slow-relaxation was slowly developed and sustained for more than thirty minutes after the administration of RGSF-A. The slow-relaxation had a tendency to be bigger than the fast-relaxation. Fast relaxation induced by RGSF-A was almost blocked by $N_{\omega}$-Nitro-L-arginine methyl ester (L-NAME), a nitric oxide synthase synthase inhibitor and 1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one (ODQ), a guanylate cyclase inhibitor. However slow relaxation induced by RGSF-A was only partially inhibited by L-NAME and ODQ. In the endothelium-removed ring, RGSF-A evoked only slowrelaxation to a certain extent. These data suggest that RGSF-A induced both endothelium dependent fast- and slow-relaxation and endothelium independent slow-relaxation in the porcine distal LAD coronary artery. The endothelium dependent fast-relaxation is mediated by the nitric oxide (NO)-cGMP pathway, and the endothelium dependent slow-relaxation is at least partially mediated by the NO-cGMP pathway. However, the endothelium-independent slow-relaxation remains to be elucidated.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.10 no.5
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• pp.1133-1140
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• 2009

The purpose of this study is to determine the most efficient method of removing sulfur dioxide from traditional herbal medicine (hanyak) by slow boiling using a traditional pipkin and an electronic slow boiler. By boiling a hanyak batch containing] 56.4ppm of sulfur dioxide for one hour using a traditional pipkin, 96.7 percent of the sulfur dioxide was removed, while two hours of slow boiling removed ]00 percent. Among different cover materials placed over the pipkin during the slow boiling process including the traditional Korean paper (hanji), regular hanji, filter paper and regular paper, the traditional hanji produced the best performance of sulfur dioxide removal. The initial pH level of the traditional hanji was] 0.03. After one hour of slow boiling hanyak batches in a traditional pipkin covered with traditional hanji, where each batch contained sulfur dioxide of 48ppm, 193ppm, 753ppm and 1,506ppm respectively, the pH level of the hanji cover material was reduced to 9.37, 9.14, 8.9 and 8.03 in respective cases. Our experiment using an electronic medicine slow boiler showed 82.8 percent removal of sulfur dioxide after one hour of slow boiling a hanyak batch containing 753ppm of sulfur dioxide. When hanyak batches were boiled by placing traditional hanji, filter paper, active carbon and hardwood charcoal separately in the middle area within the electronic slow boiler, the sulfur dioxide removal rate was 73.6 percent, 72.8 percent, 73.9 percent and 69.5 percent, respectively. When charcoal was added so as to remove toxic materials from the hanyak, its presence impeded the sulfur dioxide flow and thus reduced the removal efficiency contrary to our anticipation.

• Journal of Yeungnam Medical Science
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• v.7 no.1
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• pp.19-27
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• 1990

This investigation was undertaken to clarify the in vitro effect of the various stimulations, such as exercise(E), insulin(I), direct electrical stimulation(EST) and the combinations of the above, on the glucose incorporation into glycogen molecules (glycogen synthesis) of the normal slow(soleus) and fast twitch(plantaris) muscles, and the different responses of slow and fast twitch muscles to persistent overloads causing compensatory muscle hypertrophy. In resting state, slow twitch muscle has greater capacity for glycogen synthesis than fast twitch muscle, and responses of different muscle to various stimuli were differ as follows : In slow twitch muscle, the glycogen synthesis was increased by insulin, and electrical stimulation but not increased by exercise ; exercise increased insulin sensitivity and the effect of electrical stimulation. Whereas the glycogen synthesis in fast twitch muscle was increased only by the stimuli combined with E and EST, and E, I, and EST. As the result of removal of synergistic muscle, both muscles were hypertropied, and the degree of hypertrophy in response to persistent overload was higher in fast twitch muscle(182%) than slow twitch muscle(151%). In hypertrophied muscles, glycogen synthesis of soleus in any groups was lower than that of the control, but similar in plantaris. In conclusions, there were marked heterogeneity in defferent muscle fiber in the effects of exercise and insulin addition and electrical stimulation on muscle glycogen synthesis, and fast twitch muscle may be adapted more easily to that kind of persistent overload than slow twitch muscle.

• The Korean Journal of Physiology and Pharmacology
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• v.4 no.3
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• pp.227-234
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• 2000

Many reports suggest that neurotensin (NT) in the gastrointestinal tract may play a possible role as a neurotransmitter, a circulating hormone, or a modulator of motor activity. NT exerts various actions in the intestine; it produces contractile and relaxant responses in intestinal smooth muscle. This study was designed to investigate the effect of NT on motility of antral circular muscle strips in guinea-pig stomach. To assess the role of $Ca^{2+}$ influx in underlying mechanism, slow waves were simultaneously recorded with spontaneous contractions using conventional intracellular microelectrode technique. At the concentration of $10^{-7}$ M, where NT showed maximum response, NT enhanced the magnitude $(863{\pm}198%,\;mean\;SEM,\;n=13)$ and the frequency $(154{\pm}10.3%,\;n=11)$ of spontaneous contractions. NT evoked a slight hyperpolarization of membrane potential, tall and steep slow waves with abortive spikes $(278{\pm}50%,\;n=4).$ These effects were not affected by atropine $(2\;{\mu}M),$ guanethidine $(2\;{\mu}M)$ and tetrodotoxin (0.2μM). NT-induced contractile responses were abolished in $Ca^{2+}-free$ solution and reduced greatly to near abolition by $10\;{\mu}M$ of verapamil or 0.2 mM of $CdCl_2.$ Verapamil attenuated the effects of NT on frequency and amplitude of the slow waves. Taken together, these results indicate that NT enhances contractility in guinea-pig gastric antral circular muscle and $Ca^{2+}$ influx through the voltage-operated $Ca^{2+}$ channel appears to play an important role in the NT-induced contractile mechanism.

• Clinical and Experimental Reproductive Medicine
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• v.28 no.2
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• pp.121-129
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• 2001

Objective: The aim of this study is to compare the efficiency of a method for the cryopreservation of mouse blastocyst.. Methods: Mouse embryos were obtained at 2-cell stage and cultured to blastocyst stage in T6 medium supplemented with 10% fetal bovine serum. Morphologically normal blastocysts were collected and randomly divided to one control and four experimental groups. In control group, blastocysts were cultured in vitro continuously for additional two days. In group 2, blastocysts were exposed to vitrification solution (ethylene glycol) only without cryopreservation (exposure only group). In group 3, 4 and 5, blastocysts were cryopreserved by slow-freezing procedure with glycerol (slow-fteezing group) or by vitrification procedure using EM grids (EM grids group) and cryoloop (cryoloop group), respectively. Frozen blastocysts were thawed and cultured for additional two days. Twenty four hours after thawing, some blastocysts were fixed and stained with Hoechst 33342 (bisbenzimide) and the number of nuclei in each blastocysts were counted to confirm the survival of bias to cysts in experimental groups. Results: Survival rate and hatching rate of the blastocysts in slow-freezing group (24 h: 72.4% and 66.0%, 48 h: 63.2% and 64.6%) and EM grids group (24 h: survival rate 77.3%, 48 h: 70.1% and 71.4%) were significantly lower ($X^2$-test p<0.05) than those of control group (24 h: 93.4% and 86.0%, 48 h: 88.5% and 90.7%). In contrast, the survival rate and hatching rate of the blastocysts in cryoloop group (24 h: 84.1% and 84.1%,48 h 79.3% and 87.7%) is well compared with those in the control group. The mean (${\pm}SD$) cell number of blastocyst in the exposure only ($89.2{\pm}11.5$), EM grids ($85.0{\pm}10.3$) and cryoloop ($89.0{\pm}11.0$) groups, except slow-freezing group ($79.0{\pm}10.0$), were not significantly different from that of control group ($93.1{\pm}13.9$) 24 h after thawing (Student's t-test). Conclusion: This study demonstrates that higher survival rate of vitrified-thawed mouse blastocyst can be obtained using cryoloop as the embryo container at freezing rather than slow-freezing or vitrification using EM grids. The results of this study suggest that vitrification using cryoloop (with ethylene glycol) may be a preferable procedure for mouse blastocyst cryopreservation and could be applied to the human blastocyst cryopreservation.

• The Korean Journal of Physiology and Pharmacology
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• v.2 no.3
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• pp.361-368
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• 1998

The spontaneous contractions of gastric smooth muscles are regulated by slow waves, which are modulated by both nervous system and humoral agents. This study was designed to examine the effects of prostaglandin $E_2$ ($PGE_2$) on the contractile and electrical activities of antral smooth muscles in guinea-pig stomach, using an intracellular recording technique. To elucidate the underlying mechanism for its effect on contractility, ionic currents were also measured using a whole-cell patch clamp method. The basal tone by $PGE_2$ was variable, whereas the magnitude of phasic contractions was reduced ($19.0{\pm}2.1%$, n=19). The resting membrane potentials were hyperpolarized ($-4.4{\pm}0.5%$ mV, n=10), and plateau potentials were lowered ($-2.9{\pm}0.5%$ mV, n=10). In most cases, however, the initial peak potentials of slow waves were depolarized more by $PGE_2$ than those of control. The frequency of the slows wave was increased from $5.7{\pm}0.2$ cycles/min to $6.5{\pm}0.2$ (n=22). Voltage-operated $Ca^{2+}$ currents were decreased by $PGE_2$ (n=5). Voltage-operated $K^+$ currents, both Ca-dependent and Ca-independent, were increased (n=5). These results suggest that $PGE_2$ plays an important role in the modulation of gastric smooth muscle activities, and its inhibitory effects on the contractility and activities of slow waves are resulted from both decrease of $Ca^{2+}$ currents and increase of $K^+$ currents.

• The Korean Journal of Physiology and Pharmacology
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• v.28 no.2
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• pp.165-181
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• 2024

The slow and regular pacemaking activity of midbrain dopamine (DA) neurons requires proper spatial organization of the excitable elements between the soma and dendritic compartments, but the somatodendritic organization is not clear. Here, we show that the dynamic interaction between the soma and multiple proximal dendritic compartments (PDCs) generates the slow pacemaking activity in DA neurons. In multipolar DA neurons, spontaneous action potentials (sAPs) consistently originate from the axon-bearing dendrite. However, when the axon initial segment was disabled, sAPs emerge randomly from various primary PDCs, indicating that multiple PDCs drive pacemaking. Ca²⁺ measurements and local stimulation/perturbation experiments suggest that the soma serves as a stably-oscillating inertial compartment, while multiple PDCs exhibit stochastic fluctuations and high excitability. Despite the stochastic and excitable nature of PDCs, their activities are balanced by the large centrally-connected inertial soma, resulting in the slow synchronized pacemaking rhythm. Furthermore, our electrophysiological experiments indicate that the soma and PDCs, with distinct characteristics, play different roles in glutamate-induced burst-pause firing patterns. Excitable PDCs mediate excitatory burst responses to glutamate, while the large inertial soma determines inhibitory pause responses to glutamate. Therefore, we could conclude that this somatodendritic organization serves as a common foundation for both pacemaker activity and evoked firing patterns in midbrain DA neurons.

• Journal of the Korea Convergence Society
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• v.11 no.5
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• pp.381-389
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• 2020

Patients, particularly elderly patients, need nursing appropriate to their characteristics and pace, whereas 'fast nursing' is actually emphasized in Korea. Hence, this study attempts to identify the characteristics of 'slow nursing' in Korea. Walker & Avant's approach to concept analysis was performed through a literature review on the concept of 'slow nursing.' A literature search and concept analysis, using the keywords of 'slow,' 'slow nursing' and 'slow prescription' in Pubmed, Riss, NDSL, DBPIA, and Google Scholar from 2000 to 2019, yielded the following attributes of 'slow nursing': 'respect for the speed of the subject,' 'carefulness,' 'comfort in the process,' 'participation' and 'consideration of the meaning of life.' Model cases were derived based on these attributes. Predisposing factors involved in 'slow nursing' include patient malfunction, psychological deflection, indifference, fear of treatment, and lack of willingness to live. The outcomes were a discovery of patients' potential capacities, recovery of confidence, health promotion, improved communication with medical staff, confidence, and self-integrity. It is expected that the development of measurement tools using the attributes of 'slow nursing' would contribute to patient nursing and research in the future.

• Proceedings of the Ginseng society Conference
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• 2006.05a
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• pp.40-51
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• 2006

To investigate the association between Korean red ginseng (KRG) intake in HIV-1 infected patients and occurrence of grossly deleted nef genes ($g{\Delta}nef$), we characterized nef genes in 10 long-term slow progressors (LTSP) infected with HIV-1 subtype B and 34 control patients. LTSP was defined whose the annual decrease in CD4 T cells was less than $20/{\mu}l$ over 10 years in the absence of antiretroviral therapy. They were treated with KRG for a prolonged period. Nef genes were amplified from peripheral blood mononuclear cells (PBMC) using nested PCR and products were sequenced directly. Patient CD4 T cell counts decreased from $444{\pm}207/{\mu}l$ to $294{\pm}177/{\mu}l$ over $136{\pm}23$ months of KRG intake. This corresponds to an annual decrease in the level of CD4 T cells of $13.3/{\mu}l$. A total of 479 nef genes were amplified from 137 PBMC samples. Nine out of the 10 patients, 47 (34.3%) out of the 137 samples, and 92 out of the 479 genes revealed $g{\Delta}nef$. The deletion extended outside the nef gene in 25 $g{\Delta}nef$ obtained from 6 patients. The proportion of samples with $g{\Delta}nef$ (34.3%) was significantly higher than 4.8% in control patients (P<0.001). In addition, it significantly increased as the duration of KRG intake prolongs (P<0.01). These data suggest the possibility that occurrence of $g{\Delta}nef$ might be associated with long-term intake of KRG.