• Title/Summary/Keyword: Skeletal muscles

Search Result 323, Processing Time 0.032 seconds

한우 c-fos 유전자의 염기서열 및 발현분석 (Sequence and Expression Analysis of c-fos Proto-oncogene in Korean Cattle (HANWOO))

  • 유성란;정행진;정기철;이준헌;조규완;최재관;나기준;상병찬
    • Journal of Animal Science and Technology
    • /
    • 제45권6호
    • /
    • pp.891-900
    • /
    • 2003
  • c-fos 유전자는 전사조절인자로서 주로 c-jun family와 결합하여 heterodimers를 형성하며 AP-1 조절 부위를 가지는 유전자들의 전사를 조절하는 것으로 알려져 있다. 이 유전자의 발현은 myoblasts를 비롯한 여러 세포의 분화와 성장을 조절하며 최근 돼지에서 육질에 영향을 미치는 근섬유와 관련된다는 보고가 있다. 본 연구는 소에서 육질과 c-fos 유전자와의 관계를 알아보기 위한 기초자료로서 총 1,443 bp의 mRNA 염기서열을 최초로 소에서 밝혔으며 여러 조직과 기관에서의 발현양상도 살펴보았다. 한우의 c-fos 유전자의 염기서열을 사람, 돼지 및 쥐와 비교하여 본 결과 각각 89.8%, 93.5%와 87.0%의 높은 상동성을 보였다. 이 유전자의 발현은 근육중 갈비에서 가장 많은 발현량을 보였고, 조직에서는 비장에서 가장 많은 발현량을 보이는 것을 알 수 있었다. 이 연구에서 밝혀진 c-fos 유전자는 SNP의 추가분석에 의해 한우에서 육질의 향상과 관련이 있는 후보유전자로 쓰일 수 있을 것으로 사료된다.

Optimization of Culture Conditions for Maintaining Pig Muscle Stem Cells In Vitro

  • Choi, Kwang-Hwan;Yoon, Ji Won;Kim, Minsu;Jeong, Jinsol;Ryu, Minkyung;Park, Sungkwon;Jo, Cheorun;Lee, Chang-Kyu
    • 한국축산식품학회지
    • /
    • 제40권4호
    • /
    • pp.659-667
    • /
    • 2020
  • Muscle stem cells isolated from domestic animals, including cows and pigs, were recently spotlighted as candidates for the production of alternative protein resources, so-called cultured meat or lab-grown meat. In the present study, we aimed to optimize the in vitro culture conditions for the long-term expansion of pig muscle stem cells via the screening of various signaling molecules. Pig muscle stem cells were collected from the biceps femoris muscles of 3-d-old crossbred pigs (Landrace×Yorkshire×Duroc, LYD) and cultured in minimum essential medium-based growth media. However, the pig muscle stem cells gradually lost their proliferation ability and featured morphologies during the long-term culture over two weeks. To find suitable in vitro culture conditions for an extended period, skeletal muscle growth medium-2, including epidermal growth factor (EGF), dexamethasone, and a p38 inhibitor (SB203580), was used to support the stemness of the pig muscle stem cells. Interestingly, pig muscle stem cells were stably maintained in a long-term culture without loss of the expression of myogenic marker genes as determined by PCR analysis. Immunostaining analysis showed that the stem cells were capable of myogenic differentiation after multiple passaging. Therefore, we found that basal culture conditions containing EGF, dexamethasone, and a p38 inhibitor were suitable for maintaining pig muscle stem cells during expanded culture in vitro. This culture method may be applied for the production of cultured meat and further basic research on muscle development in the pig.

한국산(韓國産) 모래무지아과(亞科)(Gobioninae) 어류의 미설골(尾舌骨)에 대한 비교연구 (Comparative Study on the Urohyal of the Subfamily Gobioninae of Korea)

  • 김익수;강언종
    • 한국어류학회지
    • /
    • 제1권1_2호
    • /
    • pp.24-34
    • /
    • 1989
  • 외부형태를 근거로 구분되어 온 잉어과(科)(Cyprinidae)의 모래무지아과(亞科)(Gobioninae)어류는 아과(亞科)의 한계가 학자 간에 일치되지 않아 분류학적으로 문제된다. 본 연구는 이의 규명을 위해 한국산 모래무지아과(亞科) 어류(魚類) 14속(屬)에 대해 두골의 형질을 재검토하던 중 미설골(urohyal)의 형질이 뚜렷한 형질전이 상태를 보여 주목되었다. 한국산 모래무지아과(亞科) 어류는 미설골의 형질상태에 의해 다음과 같은 4개의 group으로 구분되었다. 1. Coreo leuciscus, Gnathopogon group ; 수평판의 형태는 후연이 쐐기모양으로 패인 원추형이고, 그 길이는 수직판보다 길다. 2. Squalidus, Hemibarbus group ; 수평판의 형태는 뒤로 갈수록 넓어지며 후연은 뾰죽하게 뻗은 긴 마름모꼴로, 그 길이는 수직판과 거의 같다. 3. Sarcocheilichthys, Pseudorasbora, Pseudopungtungia, Pungtungia group ; 수평판의 형태는 중앙부분이 매우 넓은 마름모꼴로, 그 길이는 수직판 보다 길고, 인대가 부착되는 선단은 2분지 되지 않으며, 중앙부분이 복면을 향해 굽어 있다. 4. Ladislavia, Gobiobotia, Abbottina, Hicrophysogobio, Pseudogobio, Saurogobio group ; 수평판의 형태는 뒤로 갈수록 가늘어지는 침상 혹은 넓은 쐐기모양으로, 그 길이는 수직판보다 짧고, 선단의 인대가 부착되는 부위는 배면으로 비후되었다. 이러한 결과는 adductor mandibulae complex와 geniohyoideus 등의 근육형질이나 두부 측선감각관 등의 형질에 의한 결과와도 일치되는 것으로 주목된다.

  • PDF

삼육대 및 서울여대 재학 중인 일부 여대생의 거주형태에 따른 영양섭취상태 및 신체계측 비교 연구 (Comparison of Nutritional and Physical Status according to the Residental Type among College Women in Seoul Women's University and Sahmyook University)

  • 최경순;신경옥;허선민;정근희
    • Journal of Nutrition and Health
    • /
    • 제43권1호
    • /
    • pp.86-96
    • /
    • 2010
  • This study was conducted to investigate the effects of residence types ((1) parent house, (2) dormitory or boarding home, (3) boarding home w/o food) on the dietary habits and health status of college women (20-23 y). Overall, 46.1% of college women reported that they were economically middle class and spent 200,000-390,000 won. Additionally, 17.4% of college women considered themselves healthy, while 33.8% reported that they were not healthy. Furthermore, 32.0% of college women had experience to control their weight and 39.8% reported that they exercised to control their weight. There were no significant differences in the height and weight of the subjects according to residential type, but the amount of skeletal muscles mass ($21.0\;{\pm}\;2.6\;kg$) of the subjects that lived in dormitory or boarding home was significantly higher (p < 0.05) than that of women who lived at home. Additionally, 30.8% of students that lived in private residences, 25.0% of students that dwelled in dormitory or boarding homes and 27.7% of students that boarding home w/o food had three regular meals every day (p < 0.05). It has been reported that 18.3% of college students eat processed and instant foods due to their convenience. In the present study, intakes of energy, protein, fat, vitamin A, vitamin $B_1$, vitamin $B_2$, vitamin $B_6$, niacin, vitamin C, vitamin E and folic acid for the subjects who lived in dormitory or boarding homes was significantly higher than those of students who lived in private residences (p < 0.05). Additionally, intakes of iron, calcium, and zinc were lower in the subjects that boarding home w/o food (p < 0.05). Finally, the blood glucose level was $84.7\;{\pm}\;13.0\;mg/dL$, and differed significantly by residential types (p < 0.05); however, the average glucose levels of all subjects were within the normal range (90-110 mg/dL).

도서지역 주민의 건강상태에 관한 연구 (A Study on The Health Status of Island Community People in Island)

  • 신경림
    • 성인간호학회지
    • /
    • 제12권2호
    • /
    • pp.296-310
    • /
    • 2000
  • During the past 10 years, concern for community people's health has increased together with the changes. Public health policies and studies for community people's health, however, have influenced those in childmaternal health care to want more general coverage and studies of health and wellness. Particularly, the study of community people's health in the extent an island area is almost rare as that personal and the material benefits in this area are lacking of community people's work is large, and the basic elements of living, such as diet and elimination, are irregular due to the schedule of the tide. Thus, there are many potential health problems. In this regard, the study attempted to understand the health problems of island community people and to provide a basis for developing health promotion and health education programs. In collecting data for the study, face to face interviews were made through a structured questionnaire from October 1 to December 30, 1996. Collected data were analyzed with the SAS statistics program, descriptive statistics, t-test and ANOVA. Subjects' health status was examined by classifying into such categories as their health perception, complaints of health problem, related lifestyle, psychosocial health staus, the result of examination is as follows; 1. For subjects' health perception, 26.9% of the subject answered not sick, but not so healthy'; 30.9% thought they were healthy, while 22.9% answered that they were not healthy. 2. For the health problem complaints many complained of pains in their muscles and skeletal system, especially knee joint pain. Women's health problems related with breast and the reproductive system included 52.3% of cases doing breast self examination, while 56.55% received the cervical cancer screening test. In men's health problems, 44.2% of subjects answered that they have moderate to severe BPH(Benign Prostatic Hypertrophy) symptom. 3. There were statistically a significant difference in the degree of physical health according to marital status(p=0.0028), occupation(p=0.0442), income(p=0.0357). 4. For stress status, 17.2% was to need the intervention, 50.2% was to need observation. 5. The mean score of self-esteem was 27.7 showing a relatively high score. 6. For the rate of smoking, 37.7% used to smoke, while 28% used to take alcohol. 7. The rate of substance abuse was 45.9% of subjects. 8. Most of subjects' health behaviors included most of the acupuncture (52%). 9. The rate of subjects receiving comprehensive medical testing was 34.36% while 34.78% did after care managing behavior. 10. For the obesity grade, 53% is normal weight, low-weight 32.8%, obesity 33%. 11. For nutrition status, 78.7% illy balanced to need intervention of nutritional education. 12. For 78.7% of subjects, muscle strength and 40.7% of stretching were not good enough to need health education on physical exercises. Therefore, based on the results, appropriate health education programs need to be developed to promote health of community people on an island.

  • PDF

Role of microRNAs in myogenesis and their effects on meat quality in pig - A review

  • Iqbal, Ambreen;Jiang, Ping;Ali, Shaokat;Gao, Zhen;Liu, Juan;Jin, Zi Kang;Pan, Ziyi;Lu, Huixian;Zhao, Zhihui
    • Asian-Australasian Journal of Animal Sciences
    • /
    • 제33권12호
    • /
    • pp.1873-1884
    • /
    • 2020
  • The demand for food is increasing day by day because of the increasing global population. Therefore, meat, the easiest and largely available source of protein, needs to be produced in large amounts with good quality. The pork industry is a significant shareholder in fulfilling the global meat demands. Notably, myogenesis- development of muscles during embryogenesis- is a complex mechanism which culminates in meat production. But the molecular mechanisms which govern the myogenesis are less known. The involvement of miRNAs in myogenesis and meat quality, which depends on factors such as myofiber composition and intramuscular fat contents which determine the meat color, flavor, juiciness, and water holding capacity, are being extrapolated to increase both the quantity and quality of pork. Various kinds of microRNAs (miRNAs), miR-1, miR-21, miR22, miR-27, miR-34, miR-127, miR-133, miR-143, miR-155, miR-199, miR-206, miR-208, miR-378, and miR-432 play important roles in pig skeletal muscle development. Further, the quality of meat also depends upon myofiber which is developed through the expression of different kinds of miRNAs at different stages. This review will focus on the mechanism of myogenesis, the role of miRNAs in myogenesis, and meat quality with a focus on the pig.

Carrageenan에 의한 근통유발이 백서의 하지근과 인대에 미치는 영향 (Effect of Carrageenan-Induced Pain on Lower Limb Muscle and Ligament of Rat)

  • 이건휘;황병천;최정선;김양중;윤주영;이건목
    • 동의생리병리학회지
    • /
    • 제19권5호
    • /
    • pp.1356-1362
    • /
    • 2005
  • The purpose of this study os to investigative the effect of Carrageenan-Induced Pain on lower limb muscle and ligament of rat. To evaluate pain mechanism in muscle and ligament, pain was induced by the injection of 2% $0.1m{\ell}$ carrageenan into the left lower limb muscle of rats after rats were anesthesized with 3% enflurane. Rats were killed on 72 hours after pain induction under the anesthesia. anterior rectus femoris muscle and its ligament were removed from rat hind limb. Morphological changes of them were peformed by the observation of light and electron microscopes. In the light microscopic findings, the muscle cells were polyheadral and situated with each other without small gap in control group. nucleus of cell was seen along the cell margin, and muscle cell groups were divided by regular narrow gap in cross section. In the pain-induced group, muscle cell groups were divided each other by the irregular gap, and some of groups formed larger than other cell groups by the fusion. Intercellular gap of most cell groups were increased compared with control groups. And also, perimysium of muscle cell groups was swollen in cross section. In control group, muscle cells contacted each other closely and each cell was divided by perimysium. The intracellular gaps were not seen between myofibrills, and also striations were well defined between muscle cells in longitudinal section. In pain-induced group, muscle cells were divided by the small intracellular gaps. And also, muscle cell showed many a short cross or longitudinal intercellular gaps in longitudinal section. In light microscopic findings of control group, tendon was composed with many tendon fibers contacted each other closely without gap. The free margin of tendon was fused, and apso the tendon fibers did not invaded between muscles. In pain-induced group, tendon was divided small groups by intertendinous gap, and also the margin of tendon divided by small groups. In the free margin, tendon invaded into muscle cells, and also fibroblasts between tendon fibers were long and lance-shaped. From these results, it is suggested that pain induction by carrageenan injured rat skeletal muscle and ligament by the morphological changes.

12주간 복합운동이 비만 여대생의 신체조성 및 기초체력 향상에 미치는 영향 (The effects of combined exercise training 12weeks on body composition and basic physical strength in obese college women)

  • 김원현;김승석
    • 디지털융복합연구
    • /
    • 제14권4호
    • /
    • pp.471-478
    • /
    • 2016
  • 본 연구의 목적은 12주간 복합운동이 비만 여대생의 신체조성 및 기초체력 향상에 미치는 영향을 규명하는데 있다. 본 연구의 취지를 충분히 설명하고 자발적 참여의 동의서를 작성한 D대학교의 여대생 12명이었으며, 과거병력과 현재 특별한 질환이 없고, 규칙적인 운동경험이 없는 자들로 구성하였다. 이들은 실험 전 신체조성검사 및 기초체력검사인 좌 우악력, 배근력, 유연성, 순발력, 근지구력을 측정하고, 12주간 복합운동 실시 후 평균 표준편차를 산출하기 위하여 기술통계를 실시하였으며, 실험 전, 후 차이 검증은 paired t-test를 이용하여 분석하였다. 통계적 유의수준은 p<.05로 설정하여 다음과 같은 결론을 얻었다. 12주간 복합트레이닝 운동집단에서 참여 후 체중, 골격근, 체지방량 및 좌 우악력 및 배근력, 순발력은 통계적으로 유의(p.<05)한 감소와 증가를 보였고, 유연성은 약간의 증가를 보였으나 통계적으로 유의한 수준의 변화를 보이지 않았으며, 통제집단은 유의한 변화가 없었다. 이상을 종합해 보면 12주간 복합운동은 여대생의 근육을 증가시켜 기초대사량이 증가하여 체중 및 체지방을 감소시켜 건강관련 기초체력 향상에 의미 있는 운동프로그램이 될 수 있을 것이라고 사료된다.

Purification of Pig Muscle Stem Cells Using Magnetic-Activated Cell Sorting (MACS) Based on the Expression of Cluster of Differentiation 29 (CD29)

  • Choi, Kwang-Hwan;Kim, Minsu;Yoon, Ji Won;Jeong, Jinsol;Ryu, Minkyung;Jo, Cheorun;Lee, Chang-Kyu
    • 한국축산식품학회지
    • /
    • 제40권5호
    • /
    • pp.852-859
    • /
    • 2020
  • The muscle stem cells of domestic animals are of interest to researchers in the food and biotechnology industries for the production of cultured meat. For producing cultured meat, it is crucial for muscle stem cells to be efficiently isolated and stably maintained in vitro on a large scale. In the present study, we aimed to optimize the method for the enrichment of pig muscle stem cells using a magnetic-activated cell sorting (MACS) system. Pig muscle stem cells were collected from the biceps femoris muscles of 14 d-old pigs of three breeds [Landrace×Yorkshire×Duroc (LYD), Berkshire, and Korean native pigs] and cultured in skeletal muscle growth medium-2 (SkGM-2) supplemented with epidermal growth factor (EGF), dexamethasone, and a p38 inhibitor (SB203580). Approximately 30% of total cultured cells were nonmyogenic cells in the absence of purification in our system, as determined by immunostaining for cluster of differentiation 56 (CD56) and CD29, which are known markers of muscle stem cells. Interestingly, following MACS isolation using the CD29 antibody, the proportion of CD56+/CD29+ muscle stem cells was significantly increased (91.5±2.40%), and the proportion of CD56 single-positive nonmyogenic cells was dramatically decreased. Furthermore, we verified that this method worked well for purifying muscle stem cells in the three pig breeds. Accordingly, we found that CD29 is a valuable candidate among the various marker genes for the isolation of pig muscle stem cells and developed a simple sorting method based on a single antibody to this protein.

Cyclophosphamide와 Prednisolone 병합요법에 치료반응을 보인 다발성근염에 동반된 간질성폐질환 1예 (Interstitial Lung Disease Associated with Polymyositis: Response to Cyclophosphamide and Prednisolone Combination Treatment)

  • 문종호;박준영;이상무;김현태;어수택;정연태;김용훈;박춘식;이경수;강대영
    • Tuberculosis and Respiratory Diseases
    • /
    • 제40권2호
    • /
    • pp.197-202
    • /
    • 1993
  • 저자들은 37세의 여자에서 스테로이드치료에 저항을 보이다가 prednisolone과 cyclophosphamide 병합투여로 치료반응을 보인 간질성폐질환을 동반한 다발성근염 1예를 경험하였기에 이를 문헌 고찰과 함께 보고하는 바이다.

  • PDF