• Title/Summary/Keyword: Size class structure

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Denaturation and Inactivation of Antioxidative Enzymes due to Repeated Exposure to UV-B and Inhibitory Effect of RGP Lens (UV-B 반복노출에 따른 항산화효소의 변성 및 활성저하와 RGP렌즈의 차단효과)

  • Byun, Hyun Young;Lee, Eun Jung;Oh, Dae Hwan;Kim, So Ra;Park, Mijung
    • Journal of Korean Ophthalmic Optics Society
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    • v.20 no.2
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    • pp.237-246
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    • 2015
  • Purpose: The present study was conducted to reveal the correlation of structural denaturation and decrease of enzyme activity when the antioxidative enzymes, superoxide dismutase (SOD) and catalase (CAT) were repeatedly exposed to UV-B, and further investigate whether the denaturation and inactivation of those enzymes can be effectively blocked by using UV-inhibitory RGP lens. Methods: Each enzyme solution was prepared from the standardized SOD and CAT, and repeatedly exposed to UV-B of 312 nm for 30 minutes, 1 hour and 2 hours a day over 1, 2, 3, 4 and 5 days. Structural denaturation of SOD and CAT induced by repeat UV-B irradiation was confirmed by the electrophoretic analysis, and their enzyme activity was determined by the colorimetric assay using the proper assay kit. At that time, the change in structure and activity of the antioxidant enzymes directly exposed to UV-B was compared to the case that UV-B was blocked by UV-inhibitory RGP lens. Results: SOD exposed repeatedly to UV-B showed the polymerization pattern in the electrophoretic analysis when it repeatedly exposed for 30 min a day, however, the change of its activity was less than 10%. On the other hand, CAT repeatedly exposed to UV-B reduced size and density of the electrophoretic band which indicated a structure denaturation, and its activity was significantly decreased. In the case that the repeat exposure time was longer, CAT activity was completely lost even though some enzyme band occurred in the electrphoretic analysis. In addition, the degeneration of CAT due to UV-B irradiation was inhibited to some extent by using RGP lens with a UV-B blocking of 63.7%, however, it was not completely inhibited. Conclusions: From these results, it was revealed that the structural denaturation of antioxidative enzymes was not perfectly correlated with the reduction in enzyme activity according to the type of enzyme. It is recommended to minimize the exposure time to UV when wearing contact lens, or wear the contact lenses having UV blocking rate of the FDA Class I blocker or the sunglasses having equivalent UV-blocking rate for reducing the damage of antioxidative enzymes induced by UV.

Characteristics of New Microsporidia S80 Isolated from Silkworm, Bombyx mori L. in Korea (가잠(家蠶)으로부터 분리(分離)된 새로운 Microsporidia S80의 특성(特性))

  • Lim, Jong Sung;Cho, Sae Yun
    • Current Research on Agriculture and Life Sciences
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    • v.1
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    • pp.67-83
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    • 1983
  • The new microsporidia S80 isolated from, Bombyx mori L. in Korea showed ovoid in the morphology of the spores and the size were measured $2.9{\pm}0.28{\mu}$ in length and $1.7{\pm}0.29{\mu}$ width. No other microsporidian spore like this has not been so far isolated from Silkworm. The length of the polar filament extruded in hydrogen peroxide ($H_2O_2$) at $30^{\circ}C$ was $26{\mu}$ of a round cytoplasm on the top. The spores were partly stained with Giemsa, Safranin-O and Gram as the same staining properties as Nosema bombycis, Microsporidia K 79 and other microsporidian spores. The fine structures were observed under scanning eleceron microscope through ultrathin sectioning. The spore wall was composed of three layers ; the thin exospore of an electron dense rippled layer, the thick electron lucent endospore which was thinning considerably at the polar filament insertion point, and the inner limiting membrane. Polar cap present at the sporeapex, with a long polar filament of 12-13 coils, subtending angle of $60^{\circ}$ to spore axis, which is tubular made up of a multilayered and are a benes core, light ring structure enclosing the dance core, the dark ring structure enclosing the inner light ring structure and the other than and light ring structure bounded from cytoplasm. Lamellate polaroplast occupied the anterior part of the spore, and the two neclei with dense nucleoplasm bounded by a double nuclear envelope were cited in the slight downer middle portion of spore. From the characteristics of the shape, size and fine structures, it is certain to reason the Microsporidia S80 belong to the phylum Microspora, class Microspora, order Microsporida, order Microsporida. The shape of two nuclei cited seems to be genus Nosema, but in the classification for the suborder it should be defined wheather pansporoblasts be formed or not and for the genis especial attempts have been made to define the characters which distinguish the disporous genera in the life cycle. Survey through the infection of the bad cocoons during 1980 to 1982 in South Korea the areas contaminated with new microsporidia were revealed 5 provinces of Kyung-Gi, Kang-Won, Chung-Nam and Chun-Nam. Pathological effects inoculated per os at second instar larvae of silkworm, the LD 50 was $7.1{\times}10^7/ml$ as lower pathogenecity than that of Nosema bombycis Naegeli of $1.2{\times}10_7/ml$. While on the other hand the inoculation of the microsporidia at fourth instar larvae lowerd the whole cocoon weight and cocoon shell weight and significant at 1% level. The microsporidia S80 defined it can not be transmitted transovarially from the result of predictive and collective examination of 21 egg batches from the infected female moth.

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Development of Habitat Suitability Index for Habitat Restoration of Narrow-mouth Frog(Kaloula borealis) (맹꽁이 서식처 복원을 위한 서식처 적합성 지수(HSI) 개발)

  • Shim, Yun-Jin;Cho, Dong-Gil;Park, Sohyun;Lee, Dong-Jin;Seo, Yun-Hee;Kim, Sang-Hyuk;Kim, Duck-Ho;Ko, Sang-Beom;Cha, Jin-Yeol;Sung, Hyun-Chan
    • Journal of the Korean Society of Environmental Restoration Technology
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    • v.17 no.2
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    • pp.109-123
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    • 2014
  • Kaloula borealis is the species of Amphibia which belongs to Kaloula genus and it is the only species inhabiting in Korea. The population size and habitat of Kaloula borealis have been significantly decreased on a national scale due to the diversified developments and the uses of agricultural pesticides. Accordingly, the Ministry of Environment has designated and managed them as the class II of endangered species, in accordance with "Endangered Species Protection and Management Act"; however, a particular study focused on the ecological restoration of Kaloula borealis is desperately needed to prevent their extinction. This study was conducted to propose the HSI (Habit Suitability Index) of Kaloula borealis based on literature survey on ecology and habitats of Kaloula borealis, as well as their HSI. Factors to be investigated in HSI include: space, feed, cover, water(breeding) and threatening factors and the variables of each factor were also proposed. The distance from wetland, grassland, farm, stream and rice paddy, as well as the altitude of spawning pond were proposed as the variables of space, whereas the bed structure of forest and low-rise grassland were proposed as the variables of feeding. The variables of water (breeding) include the area of permanent and temporary wetlands, coverage of emerged pants (ratio of open water), water depth, water temperature, water quality, pH level, etc., whereas the presence of predator, distance from street and pollutants were proposed as the variables of threatening factor. The sub-standards by HSI factor of Kaloula borealis have been drawn from in-depth consultation with experts and based on this, the final HSI of Kaloula borealis was developed.

A study on the characteristics for temporary ventilation of long subsea tunnels - focused on the current situation and improvement requirements (초장대 해저터널의 공사중 환기 특성에 관한 기초연구 - 현황 및 개선필요사항 중심)

  • Jo, Hyeong-Je;Chun, Kyu-Myung;Kim, Jong-Won;Lee, Ju-Kyung
    • Journal of Korean Tunnelling and Underground Space Association
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    • v.17 no.2
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    • pp.153-166
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    • 2015
  • Long subsea tunnel to be built below the seabed, as compared to the general railway tunnel, is subject to many restrictions in terms of spatial limitation when vertical or inclined shafts are built for the purpose of ventilation and fire safety. So, the construction of some artificial island is required to provide ventilation. But, because of construction difficulty and cost increase, it is necessary to minimize the artificial island construction. The longer ventilation distance is, the more fresh air requirement is needed. When supply airflow becomes excessive, duct size is restricted by the limitations of structure clearance and fan pressure and power increase exponentially. Therefore, in order to build a long subsea tunnel, it is necessary to overcome these practical problems and to develop technical solution that can keep the comfortable condition of tunnel environment during construction. In this study, as on ventilation method development suitable for long subsea tunnel, through comparison of temporary ventilation capacity calculation methods during construction phase, domestic and abroad, the application of Swiss SIA 196 code is found suitable for long subsea tunnel. And, through experiment on leakage of the duct connector, we confirmed that the leakage ratio per 100 m of domestic duct connection type is between 1.5~3.0%. Based on S-class duct of SIA 196 code, ventilation distance is 10.2 km, So, ventilation distance can be longer if duct connection method is improved. So, we confirmed that the improvement of leakage ratio is key issue in the construction-phase ventilation of long subsea tunnel.

A Study on the Correlation between Spatial Distance and Cognitive Intensity of High-rise Buildings - Focusing on High-rise Buildings of More than 30 Stories in Seoul - (초고층 랜드마크의 공간적 거리 및 인지강도와의 상관성 분석 - 서울시 30층 이상 고층건물을 대상으로 -)

  • Byeon, Jae-Sang;Im, Seung-Bin;Joo, Shin-Ha
    • Journal of the Korean Institute of Landscape Architecture
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    • v.35 no.4
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    • pp.90-104
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    • 2007
  • Landmarks like high-rise building are one of the important elements for the enhancement of city identity and provides the hierarchy of city streets, playing a central and symbolic role in cities. Research on physical attribute of landmarks, such as height, distance, location and shape, which are suitable for a city scale, can help a city create a distinct image and maintain comprehensible structure. To achieve this purpose, it is necessary to understand the spatial and cognitive characteristics of a landmark for the establishment and management of it. The results of this study can be summarized as follows: 1. The level of representativeness of a landmark increases in proportion to the degree of cognitive intensity on it. The relation between representativeness and cognitive intensity can be explained as the log-function as follows: Log(the number of people who respond "It has representativeness")=$-1.2579+1.5908{\times}$(cognitive intensity); 2. There are a few differences based on the attributes of respondents: while gender has no distinct influence, residential period and age show statistically meaningful influence on cognitive intensity of a vertical landmark Cognitive intensity of an individual landmarks especially, differs according to the class of main users. Because of frequent changes in occupation or employment, respondents consider the distance from a residential area more important than the distance from a working area in evaluating cognitive intensity of landmarks; 3. landmark can be classified into two kinds: a district landmark and an urban landmark A district landmark is closely connected with physical attributes of the landmark itself, such as distance, size and height. An urban landmark is mainly related to cognitive attributes such as the image and identity of a city as a whole. As a result, the landmark analysis data in this research provides spatial order and identity in a city. It is difficult to establish and reinforce the image of a city as a single element ike a landmark. However, withy steady follow-up research, this study could be seen as a systematic and logical model to improve urban landscape and image.

Meiobenthic Animals of the Tidal Flat Near the Yeonggwang Nuclear Power Plant (영광원전 주변 해역의 조간대 갯벌에 서식하는 중형저서생물)

  • Kim, Dong-Sung;Choi, Jin-Woo;Kang, Rae-Seon
    • Ocean and Polar Research
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    • v.23 no.2
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    • pp.109-119
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    • 2001
  • Meiobenthic community structure of tidal flats near the Yeonggwang Nuclear Power Plant have studied during summer (June) and fall (October) 1997. Examination of sediment samples collected along the transects showed that there were 18 different types of meiobenthos in the study area. The most abundant meiobenthic animals belonged to Phylum Nematoda in both seasons and all transects. However, sediment samples collected near discharge areas, transects A and B, showed relatively lower abundance than other general coastal areas. Another abundant meiobenthic organism is benthic Harpacticoids which is very sensitive to any environmental changes. Polycheats and Ostracods were next abundant meiobenthos which also showed the difference between the study area and other general coastal areas. Only transect C maintained similar meiobenthic abundance and diversity to other coastal areas. Horizontal distribution for transects A and B showed higher densities in upper and mid tidal flat zones. On the other hand, transect C which is located furtherest from the discharges showed an increasing trend in abundance from upper to lower areas. For size distribution analyses showed that animals which fit into the meshsize of 0.125 mm were abundant, Vertical distribution of meiobenthic animals within the sediments for both sampling seasons showed the highest individual numbers in the surface sediment layers of 0-1 cm depth and showed a decreasing trend as sediment gets deeper. Each class of meiobenthos had different vertical profiles. When comparing survey transects A and B with other similar tidal flat areas, this sites seems to a very unstable environment of tidal flats near the Yeonggwang Nuclear Power Plant.

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Basic Study on 250KW-class IoT Electric Heater for Ship Fuel Heating (선박연료 가열용 250KW급 판형 IoT 전기히터의 기초연구)

  • Jang, Sung-Cheol
    • Journal of Internet of Things and Convergence
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    • v.7 no.3
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    • pp.63-68
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    • 2021
  • Domestic electric heater manufacturers are focusing on technology development to improve the efficiency of existing products. The electric heater for ship fuel is large in size and has high difficulty in miniaturization/high efficiency, so there are not many companies that try to develop technology yet. The existing electric heater has a structure of low heating efficiency because the contact time with the heat exchange tube of the heating medium is very short because the flow path of the ship engine fuel is monotonous. Since it is manufactured in a tubular shape, the volume is very large regardless of the heating efficiency. As a result, the tubular electric heater device applied by overseas advanced companies has difficulty in maintaining and repairing because it is necessary to decompose all tubular heaters when a specific part of the inside is damaged and if the heat exchange tube is damaged, all the heat exchange tubes must be decomposed. In this study, a basic study on plate type electric heaters capable of heating 10 tons of ship engine fuel per hour and setting a maximum temperature of up to 150℃ was conducted through a 250kW capacity plate type electric heater.

A study on the 3-step classification algorithm for the diagnosis and classification of refrigeration system failures and their types (냉동시스템 고장 진단 및 고장유형 분석을 위한 3단계 분류 알고리즘에 관한 연구)

  • Lee, Kangbae;Park, Sungho;Lee, Hui-Won;Lee, Seung-Jae;Lee, Seung-hyun
    • Journal of the Korea Convergence Society
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    • v.12 no.8
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    • pp.31-37
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    • 2021
  • As the size of buildings increases due to urbanization due to the development of industry, the need to purify the air and maintain a comfortable indoor environment is also increasing. With the development of monitoring technology for refrigeration systems, it has become possible to manage the amount of electricity consumed in buildings. In particular, refrigeration systems account for about 40% of power consumption in commercial buildings. Therefore, in order to develop the refrigeration system failure diagnosis algorithm in this study, the purpose of this study was to understand the structure of the refrigeration system, collect and analyze data generated during the operation of the refrigeration system, and quickly detect and classify failure situations with various types and severity . In particular, in order to improve the classification accuracy of failure types that are difficult to classify, a three-step diagnosis and classification algorithm was developed and proposed. A model based on SVM and LGBM was presented as a classification model suitable for each stage after a number of experiments and hyper-parameter optimization process. In this study, the characteristics affecting failure were preserved as much as possible, and all failure types, including refrigerant-related failures, which had been difficult in previous studies, were derived with excellent results.

A Study on the Arrangements of YangjinDang in Sang-ju Foundation by Date on the Excavation and Jungsuki (중수기 및 발굴 자료로 본 상주 양진당의 배치에 관한 연구)

  • Kim, Chan-Yeung;Chung, Myung-Sup
    • Journal of architectural history
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    • v.20 no.4
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    • pp.61-80
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    • 2011
  • This study estimated the prototype of Yangjindang at the time of its foundation by putting together the literature and discovered data and historical research on family related to Sangju Yangjindang, and looked at its architectural characteristics. These are summarized as follows: First, Yangjindang is an office building which was completed in three years [1629] after its start of construction when Keomgan Jojeong was at the age of 72 [1626] in his latter days and it was used for performing ancestral rites for Jojeong's forefathers of the head family of Pungyang Jo by family origin. Yangjindang was founded as a base of utopia for putting ancestral rites & commemoration, harmoniousness of a tribe, and educational idea into practice together with Ojakdang. Such a movement can be judged to interpret and apply the circumstances of the times realistically and flexibly where they tried to pursue the promotion of Confucianism & studies of the proprieties as well as the consciousness of practice, and to bring a tribe into harmony after the war through the retirement of Toegye School. Second, it is located at a topographically ideal spot on the edge of the Jangcheon-a tributary of Nakdong with a good physiology and landscape and its location was also the lot for a house of Jojeong's ancestor, which was burned down by war. Behind such a location and planning of Yangjindang, it is presumed, though not certain that it was modelled after Naeap village at Andong- Jojeong's parents-in-low's home. Third, as for its foundation size, it's a head house as much as about more than 100-kan, and its structure is composed of Samyo, Bonche, and Yangjindang. In addition, arrangements of buildings and its composition system and renovation procedures followed Chu-tzu Garyoe. Composition of Samyo can be restored to Yangjindang, Jugo, Woesammun, and Samyo; however, there has been no case of existence in case of Jugo building composition & arrangement takes on an aspect of a compromise between Gamyojido and Sandangjido of Garoe, which seems to be the result from flexibly interpreting and applying the rituals and studies of the proprieties of Toegye School in keeping with locational topography and realistic circumstances while making it a principle for them to observe by Toegye School. There exists a difference between Bonche and its counterpart of the upper class housing at Sangju district in that Bonche[main building] is a squre-shaped 'Ttuljip' typical of Andong setting a family ancestral ritual as a main function. Fourth, there existed a lot of hardships in raising money to cover repairs in time of doing repairs to this structure after 180 years since its establishment. In case of the repair work on Bonche, the level of renovation was limited to the replacement of old materials for rafters, doorpost, roof members and railings with new materials, together with partial alterations in case of window system. It is estimated that Yangjindang was renovated in 1808, and afterwards it was renamed Okryujeong after being re-built at another site. Through the repairs, the floor was expanded for the clan's meeting, and angle rafters and roof members were mended as well. Especially, the plane and structure of Okryujeong which was re-built at another site are expected to give clues to its restoration due to the resemblance to original appearance of Yangjindang at the time of its renovation in 1808.

The Structure of Plant Community in Jungdaesa-Birobong Area, Odaesan National Park (오대산국립공원 중대사-비로봉 구간 식물군집구조)

  • Han, Bong-ho;Choi, Jin-woo;Noh, Tai-hwan;Kim, Dong-wook
    • Korean Journal of Environment and Ecology
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    • v.29 no.5
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    • pp.764-776
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    • 2015
  • This study aims to identify the structure of the plant community, and the ecological succession sere and the change in the forest ecosystem in Jungdaesa-Birobong area, Odaesan National Park_(i._e., located at high altitudes(over 1,000m)). It seeks to offer the basic data for the planning of vegetation management. In order to verify the status of the forest vegetation between Jungdaesa-Birobong, seventeen plots(size is $20m{\times}20m$) were set up as research sites at high altitudes. Importance value, distribution by diameter at breast height(DBH), the growth volume and age of the sample trees, similarity index and species diversity index of each survey plot were analysed. According to the results of DCA(Detrended Correspondence Analysis), one of the multivariate statistical techniques. It was found that the plant communities were classified into five groups: community I_(Quercus mongolica-Tilia amurensis community), community II_(Q. mongolica-Deciduous broad-leaved community), community III_(Q. mongolica-Pinus koraiensis community), community IV_(Abies holophylla-Q. mongolica community) and community V_(A. holophylla-Deciduous broad-leaved community). Community I which is dominated by Quercus mongolica and Deciduous broad-leaved communities is located at an altitude of over 1,300 meters(ranging from 1,335m to 1,495m), the community IV and V which are dominated by Abies holophylla are located at an altitude of under 1,200 meters(ranging from 1,115m to 1,175m) and the community II and III which include the main species of Quercus mongolica, Pinus koraiensis and Abies holophylla are located at an altitude of between 1,160 meters and 1,300 meters. The results showed that Quercus mongolica tends to have a higher importance value of woody species at a higher altitude while Abies holophylla tends to have higher importance value at a lower altitude. For the importance value woody species and -DBH class distribution, the communites I, II and III are expected to continuously maintain the present status. Whereas, for the influence of communities IV and V, Q. mongolica is predicted to be weakened. The age of sample trees was between 85 and 161; the average age was 123. The index of Shannon's Species diversity (H') showed heterogeneity was found among community I_(i._e., located at high altitude) and communities IV and V_(i._e., located at low altitude). As a results of analysing the index of Shannon's Species diversity (H': unit: $400m^2$), community III showed the highest diversity intex with 1.1109 followed by community II with 1.0475, community I with 1.0125, community IV with 0.9918 and community V with 0.8686. This study verified that the index of Shannon's species was significantly different by plant communities. For instance, when comparing the index of Shannon's species diversity in Quercus mongolica communities of this study and that of past relevant research, the value of index is very similar. However, the diversity index for the community which is dominated by Abies holophylla showed lower value when compared to the results from past relevant research.