• International Journal of Industrial Entomology and Biomaterials
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• v.6 no.2
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• pp.157-162
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• 2003

The glutathione S-transferase (GSTs) are enzymes responsible for the protection of cells from chemical toxicants and oxidative stress. We describe here the cDNA sequence and mRNA expression of a putative GST from the mole cricket, Gryllotalpa orientalis. The G. orientalis GST cDNA sequences comprised of 621 bp encoding 207 amino acid residues. The multiple sequence alignment of G. orientalis GST gene with other known insect GSTs showed several conserved residues that may be essential for the enzymatic activity of the protein. Phylogenetic analysis of the deduced amino acid sequences of G. orientalis GST gene with other insect GST sequences revealed that the G. orientalis GST gene belongs to class I GST, forming a strong monophyletic group (100% bootstrap value) exclusively for class I GSTs from a diverse insect species. Northern blot analysis confirmed midgut-specific expression at transcriptional level, evidencing the midgut as a site for GST synthesis.

• Asian Pacific Journal of Cancer Prevention
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• v.13 no.6
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• pp.2735-2738
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• 2012

Background: Our objective was to clarify the clinical and biological characteristics of basal-like breast cancer (BLBC) and non-basal-like breast cancer (TN3BKE) in Heilongjiang. Methods: We examined, by immunohistochemistry, expression of biological markers cytokeratin (CK) 5/6 and epidermal growth factor receptor (EGFR) and B cell specific moloney murine leukemia virus integration site 1( Bmi-1) in triple-negative breast cancer (TNBC). We studied the correlation between BLBC and several factors related to tumor progression, along with its prognostic value. Results: In the 229 cases of operable TNBC, BLBC was detected in 178 (77.7%) and TN3BKE- in 51 (22.2%). There was no significant difference in clinicopathological factors between them, However, BLBC was significantly associated with Bmi-1 expression (P=0.000) and shorter disease-free survival (DFS) (P = 0.045) and overall survival (OS) (P = 0.041). Conclusions: Compared with the non-basal group, patients with BLBC have a high expression of Bmi-1 and a poor prognosis.

• Journal of Korean Society on Water Environment
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• v.21 no.5
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• pp.470-476
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• 2005

The Korean Water Quality Index (K-WQI) was applied to the rivers located in the watershed of Daecheong Lake to assess the status of river water quality, and propose potential target constituents for better water quality management in the watershed. The estimated K-WQI value for each river was varied from 70 to 90, and Youngdongcheon showed the worst score while Mujunamdeachen showed the best score. The total nitrogen (TN) and total coliform bacteria were identified as the most significant constituents that degrade the K-WQI values in the rivers. The correlation coefficients (r) were determined between K-WQI and the delivered specific load ($kg/km^2/yr$) of BOD, TN, and TP to justify potential target constituents that have a great influence on the improvement of K-WQI values. The results showed that TN (r=-0.86) and TP (r=-0.85) have a strong negative relationships with K-WQI, but BOD have almost no effect. This implies that BOD, the surrogate parameter for organic pollutants, is no more a feasible water quality variable for the water quality management in the study site.

• Proceedings of the Korean Geotechical Society Conference
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• 2004.03b
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• pp.245-252
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• 2004

When some enormous earthquake hazards broke out in the neighboring Japan and Taiwan, many Korean earthquake engineers thought that seismic guidelines must be adjusted safely and economically to consider the moderate earthquake characteristics. In the present aseismic guideline for liquefaction potential assessment, a simplified method using SPT-N value and a detail method based on the dynamic lab-tests were introduced. However, it is said that these methods based on the equivalent stress concept to simplify an irregular earthquake are not reliable to simulate the kaleidoscopical characteristics of earthquake loading correctly. Especially, even though various data from the dynamic lab-test can be obtained, only two data, a maximum cyclic load and a number of cycle at an initial liquefaction are used to determine the soil resistance strength in the detailed method. In this study, a new assessment of liquefaction potential is proposed and verified. In the proposed assessment, various data from dynamic lab-tests are used to determine the unique soil resistance characteristic and a site specific analysis is introduced to analyze the irregular earthquake time history itself. Also, it is found that the proposed assessment is reasonable because it is devised to reflect the changeable soil behavior under dynamic loadings resulted from the generation and development of excess pore water pressure.

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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• 2008.11a
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• pp.241-242
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• 2008

The Velocity Autocorrelation Function (VAF) of the sodium ions is calculated for a range of temperature from 250K to 1000K and converted into the linear ac-conductivity and ac-susceptibility response via Fourier transformation. A peak is found in the conductivity around $6\times10^{12}$ Hz that has some of the character of a Poley absorption. Here it is shown to be due to an harmonically coupled site vibrations of the sodium atoms, which extend only over a limited range. At frequencies below the peak the conductivity tends towards a constant i.e. dc value corresponding to a constant flow of ions through the simulation cell. At high temperatures the conductivity due to this ion transport process behaves like a metal with an insulator to metal transition occurring around a specific temperature.

• Journal of Radiation Protection and Research
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• v.26 no.3
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• pp.299-303
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• 2001

Using ultrasound techniques, the Korea Atomic Energy Research Institute has measured chest wall thicknesses of a group of male workers at the Korea Atomic Energy Research Institute. A site-specific biometric equation has been developed for these workers. Chest wall thickness is an important modifier on lung counting efficiency. These data have been put into the perspective of the ICRP recommended dose limits for occupationally exposed workers: 100 mSv in a 5-year period with a maximum of 50 mSv in anyone year. For measured chest wall thicknesses of 1.9 cm to 4.1 cm and a 30 min counting time, the achievable MDAs for natural uranium in the KAERI lung counter vary from 5.75 mg to 11.28 mg. These values are close to, or even exceed, the predicted amounts of natural uranium that will remain in the lung (absorption type M and S) after an intake equal to the Annual Limit on Intake corresponding to a committed dose of 20 mSv. This paper shows that the KAERI lung counter probably cannot detect an intake of Type S natural uranium in a worker with a chest wall thickness equal to the average value (2.7 cm) under routine counting conditions.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2010.05a
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• pp.15-15
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• 2010

Koran ginseng(Pnax ginseng) is one of the most important medicinal plants in Orient. Among the nine cultivars of Korea ginseng, Chunpoong commands a much greater market value and has been planted widely. A rapid and reliable method for discriminating the Chunpoong cultivar was developed by exploiting a single nucleotide polymorphism (SNP) in the mitochondrial nad7 intron 4 region of nine Korea ginseng cultivars using universal primers. A SNP was detected between Chunpoong and other cultivars and modified allele-specific primers were designed from this SNP site to effective method for the geneic identification of the Chunpoong cultivar of ginseng.

• Proceedings of the SAREK Conference
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• 2009.06a
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• pp.45-51
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• 2009

A ground-loop heat exchanger in a ground source heat pump system is an important unit that determines the thermal performance of a system and its initial cost. The size and performance of this heat exchanger is highly dependent on ground thermal properties. A proper design requires certain site-specific parameters, most importantly the ground effective thermal conductivity, the borehole thermal resistance and the undisturbed ground temperature. This study was performed to investigate the effect of some parameters such as borehole lengths, various grouting materials and U-tube configurations on ground effective thermal conductivity. In this study, thermal response tests were conducted using a testing device with 9-different ground-loop heat exchangers. From the experimental results, the length of ground-loop heat exchanger affects to the effective thermal conductivity. Among the various grouting materials, the bentonite-based grout with silica sand shows the largest thermal conductivity value.

• Proceedings of the KIEE Conference
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• 2008.07a
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• pp.2299-2300
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• 2008

The Velocity Autocorrelation Function (VAF) of the sodium ions is calculated for a range of temperature from 250K to 1000K and converted into the linear ac-conductivity and ac-susceptibility response via Fourier transformation. A peak is found in the conductivity around $6{\times}10^{12}$ Hz that has some of the character of a Poley absorption. Here it is shown to be due to an harmonically coupled site vibrations of the sodium atoms, which extend only over a limited range. At frequencies below the peak the conductivity tends towards a constant i.e. dc value corresponding to a constant flow of ions through the simulation cell. At high temperatures the conductivity due to this ion transport process behaves like a metal with an insulator to metal transition occurring around a specific temperature.

• Journal of Microbiology
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• v.43 no.3
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• pp.237-243
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• 2005

The protease purified from Bacillus cereus JH108 has the function of leucine specific endopeptidase. When measured by hydrolysis of synthetic substrate (N-succinyl-Ala-Ala-Pro-Leu-p-nitroanilide), the enzyme activity exhibited optimal activity at pH 9.0, $60^{\circ}C$. The endopeptidase activity was stimulated by $Ca^{++},\;Co^{++},\;Mn^{++},\;Mg^{++},\;and\;Ni^{++}$, and was inhibited by metal chelating agents such as EDTA, 1,10-phenanthroline, and EGTA. Addition of serine protease inhibitor, PMSF, resulted in the elimination of the activity. The endopeptidase activity was fully recovered from the inhibition of EDTA by the addition of 1 mM $Ca^{++}$, and was partially restored by $Co^{++}\;and\;Mn^{++}$, indicating that the enzyme was stabilized and activated by divalent cations and has a serine residue at the active site. Addition of $Ca^{++}$ increased the pH and heat stability of endopeptidase activity. These results show that endopeptidase requires calcium ions for activity and/or stability. A Lineweaver-Burk plot analysis indicated that the $K_m$ value of endopeptidase is 0.315 mM and $V_{max}$ is 0.222 ) is $0.222\;{\mu}mol$ of N-succinyl-Ala-Ala-Pro-Leu-p-nitroanilide per min. Bestatin was shown to act as a competitive inhibitor to the endopeptidase activity.