• Journal of the Korean Geotechnical Society
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• v.20 no.9
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• pp.33-45
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• 2004

Sandfill at reclaimed sites is usually formed by more than one placement method. Reclaimed sandfill often shows highly variable profiles and the cone penetration test is most commonly used for site characterization. Correlations between cone resistance and geotechnical parameters for sand are influenced by in-situ stress level and it is important to incorporate stress level effect. In this study, cone penetration tests were performed at several elevations from the top of a 10m high surcharge, which was later removed step by step. In order to establish more reliable correlations between cone resistance and geotechnical parameters for sand, different ways of normalizing cone resistance by the corresponding in-situ vertical stress were investigated.

• Membrane Journal
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• v.15 no.4
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• pp.289-296
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• 2005

In the MBR process, the membrane fouling occurs seriously on the membrane surface. In general, the membrane fouling is attributed to factors such as deposition or adhesion of sludge floc. The occurrence of fouling is a main cause of a decrease in membrane module fluk. At this study, our MBR membrane is manufactured by nano-particle with excellent anti-fouling character. The fine nano-material which can repel the sludge Hoc from the membrane surface is distributed in the membrane surface. We confirm anti-fouling effect, test continuously in the pilot site.

• Nuclear Engineering and Technology
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• v.53 no.2
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• pp.498-508
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• 2021

Micro reactors are increasingly being considered for utilization as distributed power sources. Hence, the probabilistic safety assessment (PSA) of a direct supercritical-CO₂-cooled fast reactor, called micro modular reactor (MMR), was performed in this study; this reactor was developed using innovative design concepts. It adopted a modular design and passive safety systems to minimize site constraints. As the MMR is in its conceptual design phase, design weaknesses and valuable safety insights could be identified during PSA. Level 1 internal event PSA was carried out involving literature survey, system characterization, identification of initiating events, transient analyses, development of event trees and fault trees, and quantification. The initiating events and scenarios significantly contributing to core damage frequency (CDF) were determined to identify design weaknesses in MMR. The most significant initiating event category contributing to CDF was the transients with the power conversion system initially available category, owing to its relatively high occurrence frequency. Further, an importance analysis revealed that the safety of MMR can be significantly improved by improving the reliability of reactor trip and passive decay heat removal system operation. The findings presented in this paper are expected to contribute toward future applications of PSA for assessing unconventional nuclear reactors in their conceptual design phases.

• Nuclear Engineering and Technology
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• v.51 no.1
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• pp.104-115
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• 2019

Since the Fukushima Daiichi accident, there has been an emphasis on the risk resulting from multi-unit accidents. Human reliability analysis (HRA) is one of the important issues in multi-unit probabilistic safety assessment (MUPSA). Hence, there is a need to properly identify all the human and organizational factors relevant to a multi-unit incident scenario in a nuclear power plant (NPP). This study identifies and categorizes the human and organizational factors relevant to a multi-unit incident scenario of NPPs based on a review of relevant literature. These factors are then analyzed to ascertain all possible unit-to-unit interactions that need to be considered in the multi-unit HRA and the pattern of interactions. The human and organizational factors are classified into five categories: organization, work device, task, performance shaping factors, and environmental factors. The identification and classification of these factors will significantly contribute to the development of adequate strategies and guidelines for managing multi-unit accidents. This study is a necessary initial step in developing an effective HRA method for multiple NPP units in a site.

• Journal of Nuclear Fuel Cycle and Waste Technology(JNFCWT)
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• v.21 no.4
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• pp.427-438
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• 2023

Thermodynamic sorption modeling can enhance confidence in assessing and demonstrating the radionuclide sorption phenomena onto various mineral adsorbents. In this work, Ca-montmorillonite was successfully purified from Bentonil-WRK bentonite by performing the sequential physical and chemical treatments, and its geochemical properties were characterized using X-ray diffraction, Brunauer-Emmett-Teller analysis, cesium-saturation method, and controlled continuous acid-base titration. Further, batch experiments were conducted to evaluate the adsorption properties of Cs(I) and Sr(II) onto the homoionic Ca-montmorillonite under ambient conditions, and the diffuse double layer model-based inverse analysis of sorption data was performed to establish the relevant surface reaction models and obtain corresponding thermodynamic constants. Two types of surface reactions were identified as responsible for the sorption of Cs(I) and Sr(II) onto Ca-montmorillonite: cation exchange at interlayer site and complexation with edge silanol functionality. The thermodynamic sorption modeling provides acceptable representations of the experimental data, and the species distributions calculated using the resulting reaction constants accounts for the predominance of cation exchange mechanism of Cs(I) and Sr(II) under the ambient aqueous conditions. The surface complexation of cationic fission products with silanol group slightly facilitates their sorption at pH > 8.

• Asian Pacific Journal of Cancer Prevention
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• v.13 no.11
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• pp.5605-5611
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• 2012

Background: In the last two decades, pioneering research on anti-tumour activity of saffron has shed light on the role of crocetin, picrocrocin and safranal, as broad spectrum anti-neoplastic agents. However, the exact mechanisms have yet to be elucidated. Identification and characterization of the targets of bioactive constituents will play an imperative role in demystifying the complex anti-neoplastic machinery. Methods: In the quest of potential target identification, a dual virtual screening approach utilizing two inverse screening systems, one predicated on idTarget and the other on PharmMapper was here employed. A set of target proteins associated with multiple forms of cancer and ranked by Fit Score and Binding energy were obtained from the two independent inverse screening platforms. The validity of the results was checked by meticulously analyzing the post-docking binding pose of the picrocrocin with Hsp90 alpha in AutoDock. Results: The docking pose reveals that electrostatic and hydrogen bonds play the key role in inter-molecular interactions in ligand binding. Picrocrocin binds to the Hsp90 alpha with a definite orientation appropriate for nucleophilic attacks by several electrical residues inside the Hsp90-alpha ATPase catalytic site. Conclusion: This study reveals functional information about the anti-tumor mechanism of saffron bioactive constituents. Also, a tractable set of anti-neoplastic targets for saffron has been generated in this study which can be further authenticated by in vivo and in vitro experiments.

• Genomics & Informatics
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• v.18 no.3
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• pp.28.1-28.9
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• 2020

Streptomyces coelicolor is a gram-positive soil bacterium which is well known for the production of several antibiotics used in various biotechnological applications. But numerous proteins from its genome are considered hypothetical proteins. Therefore, the present study aimed to reveal the functions of a hypothetical protein from the genome of S. coelicolor. Several bioinformatics tools were employed to predict the structure and function of this protein. Sequence similarity was searched through the available bioinformatics databases to find out the homologous protein. The secondary and tertiary structure were predicted and further validated with quality assessment tools. Furthermore, the active site and the interacting proteins were also explored with the utilization of CASTp and STRING server. The hypothetical protein showed the important biological activity having with two functional domain including POD-like_MBL-fold and rhodanese homology domain. The functional annotation exposed that the selected hypothetical protein could show the hydrolase activity. Furthermore, protein-protein interactions of selected hypothetical protein revealed several functional partners those have the significant role for the bacterial survival. At last, the current study depicts that the annotated hypothetical protein is linked with hydrolase activity which might be of great interest to the further research in bacterial genetics.

• Journal of Microbiology and Biotechnology
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• v.33 no.6
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• pp.788-796
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• 2023

Canine parvovirus type 2 (CPV-2) has high morbidity and mortality rates in canines. Nonstructural protein 1 (NS1) of CPV-2 has endonuclease activity, initiates viral DNA replication, and is highly conserved. Thus, it is a promising target for antiviral inhibitor development. We overexpressed a 41.9 kDa active recombinant endonuclease in Escherichia coli and designed a nicking assay using carboxyfluorescein and quencher-linked ssDNA as substrates. The optimal temperature and pH of the endonuclease were 37℃ and pH 7, respectively. Curcumin, bisdemethoxycurcumin, demethoxycurcumin, linoleic acid, tannic acid, and α-tocopherol inhibited CPV-2 NS1 endonuclease with IC₅₀ values of 0.29 to 8.03 µM. The extracted turmeric, yerba mate, and sesame cake suppressed CPV-2 NS1 endonuclease with IC₅₀ values of 1.48, 7.09, and 52.67 ㎍/ml, respectively. The binding affinity between curcumin, the strongest inhibitor, and CPV-2 NS1 endonuclease by molecular docking was -6.4 kcal/mol. Curcumin inhibited CPV-2 NS1 endonuclease via numerous hydrophobic interactions and two hydrogen bonds with Lys97 and Pro111 in the allosteric site. These results suggest that adding curcuminoids, linoleic acid, tannic acid, α-tocopherol, extracted turmeric, sesame cake, and yerba to the diet could prevent CPV-2 infection.

• Journal of the Korea Organic Resources Recycling Association
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• v.23 no.3
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• pp.68-77
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• 2015

This study was conducted to characterize food waste leachate (FWL) and to compare its characteristics according to generation source: feed supplement- and compost-producing facilities. FWL contained high levels of organic compounds such as carbohydrate, protein and lipid. The moisture content of FWL was among the range of conventional wet anaerobic digestion. FWL was acidic (pH of $4.0{\pm}0.3$) and showed high coefficients of variance for specific parameters: carbohydrate, protein, lipid, ethanol, acetic acid and propionic acid. FWL derived from feed supplement-producing facilities showed slightly lower concentrations of most parameters than FWL derived from compost-producers. However, the difference was not significant at 5% significance level according to analysis of variance.

• Journal of Marine Bioscience and Biotechnology
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• v.2 no.4
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• pp.224-229
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• 2007

Muscle tissue expresses many muscle-specific genes, including myostatin (also known as GDF8) that is a member of the transforming growth factor-beta superfamily. Myostatin (MSTN) negatively regulates mammalian skeletal muscle growth and development by inhibiting myoblast proliferation. Mice and cattle possessing mutant MSTN alleles display a 'double muscling' phenotype characterized by extreme skeletal muscle hypertrophy and/or hyperplasia. In this study, we first have characterized partial cDNA of a MSTN gene from the muscle tissue in the F. chinensis and examined its expression pattern in various tissues. The partial MSTN gene (GenBank accession number EU 131093) in the F. chinensis was 1134 bp, encoding for 377 amino acids that showed 63-93% amino acid similarity to other vertebrate MSTNs, containing a conserved proteolytic cleavage site (RXRR) and conserved cysteine residues in the C-terminus. Based on a RT-PCR, the MSTN gene was expressed in the all tissues of F. chinensis used in this study.