• Horticulture, Environment, and Biotechnology : HEB
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• v.59 no.6
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• pp.857-864
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• 2018

Previous studies have not detected transcripts of the gene encoding anthocyanidin synthase (ANS) in white pomegranates (Punica granatum L.) and suggest that a large-sized insertion in the coding region of the ANS gene might be the causal mutation. To elucidate the identity of the putative insertion, 3887-bp 5' and 3392-bp 3' partial sequences of the insertion site were obtained by genome walking and a gene coding for an expansin-like protein was identified in these genome-walked sequences. An identical protein (GenBank accession OWM71963) isolated from pomegranate was identified from BLAST search. Based on information of OWM71963, a 5.8-Mb scaffold sequence with genes coding for the expansin-like protein and ANS were identified. The scaffold sequence assembled from a red pomegranate cultivar also contained all genome-walked sequences. Analysis of positions and orientations of these genes and genome-walked sequences revealed that the 27,786-bp region, including the 88-bp 5' partial sequences of the ANS gene, might be translocated into an approximately 22-kb upstream region in an inverted orientation. Borders of the translocated region were confirmed by PCR amplification and sequencing. Based on the translocation mutation, a simple PCR codominant marker was developed for efficient genotyping of the ANS gene. This molecular marker could serve as a useful tool for selecting desirable plants at young seedling stages in pomegranate breeding programs.

• Journal of the Earthquake Engineering Society of Korea
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• v.25 no.1
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• pp.33-42
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• 2021

The design response spectrum presented in the seismic design standard reflects the characteristics of the tectonic environment at a site. However, since the design response spectrum does not represent the ground motion with a specific earthquake magnitude or distance, input ground motions for response history analysis need to be selected reasonably. It is appropriate to use observed ground motions recorded in Korea for the seismic design. However, recently recorded ground motions in the Gyeongju (2016) or Pohang (2017) earthquakes are not compatible with the design response spectrum. Therefore, it is necessary to convert the recorded ground motion in Korea to a model similar to the design response spectrum. In this study, several approaches to adjust the spectral acceleration level at each period range were tested. These are the intrinsic and scattering attenuation considering the earthquake environment, magnitude, distance change by the green function method, and a rupture propagation direction's directivity effect. Using these variables, the amplification ratio for the representative natural period was regressed. Finally, the optimum condition compatible with the design response spectrum was suggested, and the validation was performed by converting the recorded ground motion.

• Structural Engineering and Mechanics
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• v.82 no.3
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• pp.325-341
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• 2022

Previous major earthquakes indicated that the earthquake induced ground motions are typical non-stationary processes, which are non-stationary in both amplification and frequency. For the convenience of aseismic design and analysis, it usually assumes that the ground motions at structural supports are stationary processes. The development of time-frequency analysis technique makes it possible to evaluate the non-stationary responses of engineering structures subjected to non-stationary inputs, which is more general and realistic than the analysis method commonly used in engineering. In this paper, the wavelet-based stochastic vibration analysis methodology is adopted to calculate the non-stationary responses of multi-support structures. For comparison, the stationary response based on the standard random vibration method is also investigated. A frame structure and a two-span bridge are analyzed. The effects of non-stationary spatial ground motion and local site conditions are considered, and the influence of structural property on the structural responses are also considered. The analytical results demonstrate that the non-stationary spatial ground motions have significant influence on the response of multi-support structures.

• Earthquakes and Structures
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• v.22 no.3
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• pp.263-275
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• 2022

Structural collapses can occur as a result of a dynamic amplification of either, the building's seismic response or the ground shaking by local site effects; one of the reasons is a resonance effect due to the proximity of the structural elastic fundamental period TE and the soil fundamental period TS. We evaluate the vulnerability to resonance effects in Guadalajara, México, in a three-step schema: 1) we define structural systems in the building environment of western Guadalajara, in terms of their construction materials and structural components; 2) we estimate TE with different equations, to obtain a representative value in elastic conditions for each structural system; and, 3) we evaluate the resonance vulnerability by the analysis of the ratio between TE and TS. We observe that the larger the soil fundamental period, the higher the resonance vulnerability for buildings with height between 17 and 39 m. For the sites with a low TS, the most vulnerable buildings will be those with a height between 2 and 9 m. These results can be a helpful tool for disaster prevention, by avoiding the construction of buildings with certain heights and structural characteristics that would result in a dangerous proximity between TE and TS.

• Journal of the Earthquake Engineering Society of Korea
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• v.22 no.3
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• pp.175-184
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• 2018

In this paper, comparative analysis of the 9.12 Gyeongju and 11.15 Pohang earthquakes was conducted in order to provide probable explanations and reasons for the damage observed in the 11.15 Pohang earthquake from both earthquake and structural engineering perspectives. The damage potentials like Arias intensity, effective peak ground acceleration, etc observed in the 11.15 Pohang earthquake were generally weaker than those of the 9.12 Gyeongju earthquake. However, in contrast to the high-frequency dominant nature of the 9.12 Gyeongju earthquake records, the spectral power of PHA2 record observed in the soft soil site was highly concentrated around 2Hz. The base shear around 2 Hz frequency was as high as 40% building weight. This frequency band is very close to the fundamental frequency of the piloti-type buildings severely damaged in the northern part of Pohang. Unfortunately, in addition to inherent vertical irregularity, most of the damaged piloti-type buildings had plan irregularity as well and were non-seismic. All these contributed to the fatal damage. Inelastic dynamic analysis indicated that PHA2 record demands system ductility capacity of 3.5 for a structure with a fundamental period of 0.5 sec and yield base shear strength of 10% building weight. The system ductility level of 3.5 seems very difficult to be achievable in non-seismic brittle piloti-type buildings. The soil profile of the PHA2 site was inversely estimated based on deconvolution technique and trial-error procedure with utilizing available records measured at several rock sites during the 11.15 Pohang earthquake. The soil profile estimated was very typical of soil class D, implying significant soil amplification in the 11.15 Pohang earthquake. The 11.15 Pohang earthquake gave us the expensive lesson that near-collapse damage to irregular and brittle buildings is highly possible when soil is soft and epicenter is close, although the earthquake magnitude is just minor to moderate (M 5+).

• Asian-Australasian Journal of Animal Sciences
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• v.18 no.7
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• pp.942-948
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• 2005

New polymorphism of major histocompatibility complex B-G genes was investigated by amplification and digestion of a 401bp fragment including intron 1 and exon 2 using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique with two restriction enzymes of Msp I and Tas I in eight breeds of Chinese indigenous chickens and one exotic breed. In the fragment region of the gene, three novel single nucleotide polymorphisms (SNPs) were detected at the two restriction sites. We found the transition of two nucleotides of A294G and T295C occurred at Tas I restriction site, and consequently led to a non-synonymous substitution of asparagine into serine at position 54 within the deduced amino acid sequence of immunoglobulin variable-region-like domain encoded by the exon 2 of B-G gene. It was observed at rare frequency that a single mutation of A294G occurring at the site, also caused an identical substitution of amino acid, asparagine 54-to-serine, to that we described previously. And the transversion of G319C at Msp I site led to a non-synonymous substitution, glutamine 62-to-histidine. The new alleles and allele frequencies identified by the PCR-RFLP method with the two enzymes were characterized, of which the allele A and B frequencies at Msp I and Tas I loci were given disequilibrium distribution either in the eight Chinese local breeds or in the exotic breed. By comparison, allele A at Msp I locus tended to be dominant, while, the allele B at Tas I locus tended to be dominant in all of the breeds analyzed. In Tibetan chickens, the preliminary association analysis revealed that no significant difference was observed between the different genotypes identified at the Msp I and Tas I loci and the laying performance traits, respectively.

• Journal of the Korean earth science society
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• v.31 no.6
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• pp.541-554
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• 2010

Fifteen min-microtremor data sets were collected at 136 sites from a coastal area of Kunsan and 117 sites from an inland area of Jeonju located in SW Korea, and were analyzed for the HVSR (Horizontal to Vertical Spectral Ratio) of the sites. The microtremor spectra of the coastal area have stronger energy in the lower frequency range from 1-6 Hz than those of the coastal area. This result can be attributed to the effect of the waves and tides in the Keum river and the Yellow sea. Twenty four hours of measurement of the microtremor indicated that the microtremor spectrum correlates with the human activities, but the microtremor HVSR peak was observed consistently at the characteristic frequency for the site. The HVSR peaks were grouped into 4 types -"single peak", "double peak", "broad peak" or "no peak"- based on their shapes. More than 90% of the data sets exhibit peak frequencies ($F_0$) which can be easily identified. The distribution of $F_0$ reveals a close relationship with the topography and local geology of the areas, exhibiting high F0s in the hillside areas and low $F_0s$ in the reclaimed land area. While the amplitudes of microtremor HVSR peak frequencies are less than 4 in the downstream of the inland area, those of the recently reclaimed land in the coastal area are extremely high (more than 10). The results of this study indicate that detailed HVSR studies are essential for the earthquake hazard reduction of reclaimed lands.

• Journal of the Society of Disaster Information
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• v.16 no.1
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• pp.133-143
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• 2020

Purpose: This study aims to derive a predictive empirical equation for PGV prediction from P-wave using earthquake records in Korea and to verify the reliability of Onsite EEW. Method: The noise of P wave is removed from the observations of 627 seismic events in Korea to derive an empirical equation with PGV on the base rock, and reliability of Onsite alarms is verified from comparing PGV's predictions and observations through simulation using the empirical equation. Result: P-waves were extracted using the Filter Picker from earthquake observation records that eliminated noises, a linear regression with PGV was used to derive a predictive empirical equation for Onsite EEW. Through the on-site warning simulation we could get a success rate of 80% within the MMI±1 error range above MMI IV or higher. Conclusion: Through this study, the design feasibility and performance of Onsite EEWS using domestic earthquake records were verified. In order to increase validity, additional medium-sized seismic observations from abroad are required, the mis-detection of P waves is controlled, and the effect of seismic amplification on the surface is required.

• Korean Journal of Microbiology
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• v.33 no.2
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• pp.149-156
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• 1997

We have employed comparative RFL,P(Restriction Fragment Ixngth Pol~iniorphism) analysis and molecular phylogenetic techniques to investigate the diversity of uncultured microorganisms associated with the anaerobic PCP degradation in PCP-adapted enrichment cultures inoculated by samples from anaerobic cewage sludgc(Jangrim, Pusan) and leachate of landfill site(Kimhae). 16s rDNA cloncs were obtairted by PCR amplification of mixed population DNAs extracted directly from the nonactive and active stage ol each PCP-adapted culture. After three rounds of comparative RFLP analyses. two RFLP types. designated as Ala and Hld, were found prevalent and common in both active stage samples. Thc analysis of phylogenctic diversity bawd on the 5'-terminal 180 nt of sequences from whole clones of the Ala and Bld RFLP types showed close similarity among themselves. In case of Bld clones, 7XQ of them shared identical sequences. Thcse resuliq suggest that the clones of both RFLP types wcre originated from highly affiliated microorganisms which are e~iriched as a result of metabolic activity to PCP. The full-length 16s rRNA sequence of each representative clone from both RFLP types was determined. and an Ala clone w i n found to he related to Clo.strrdiurn ulfutzac~(Genk~ank No. Z69203) and a Bld clone to Thermobacteroides proteolyticus(Genbank No. X09335), with sequence similarities of 89%' and 97%. respectively.

• Journal of the Korean GEO-environmental Society
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• v.20 no.5
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• pp.5-12
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• 2019

On November 15, 2017, a unpredictable liquefaction damage was occurred at the $M_L=5.4$ Pohang earthquake and after, many researches have been conducted in Korea. In Korea, where there were no cases of earthquake damage, it has been extremely neglectable in preparing earthquake risk maps and building earthquake systems that corresponded to prevention and preparation. Since it is almost impossible to observe signs and symptoms of drought, floods, and typhoons in advance, it is very effective to predict the impacts and magnitudes of seismic events. In this study, 14,040 borehole data were collected in the metropolitan area and liquefaction evaluation was performed using the amplification factor. Based on this data, liquefaction hazard maps were prepared for ground accelerations of 0.06 g, 0.14 g, 0.22 g, and 0.30 g, including 200years return period to 4,800years return period. Also, the correlation analysis between the earthquake acceleration and LPI was carried out to draw a real-time predictable liquefaction hazard map. As a result, 707 correlation equations in every cells in GIS map were proposed. Finally, the simulation for liquefaction risk mapping against artificial earthquake was performed in the metropolitan area using the proposed correlation equations.