• 제목/요약/키워드: Single-strand conformation polymorphism

검색결과 69건 처리시간 0.026초

Asymmetric Polymerase Chain Reaction-Single-Strand Conformation Polymorphism (Asymmetric PCR-SSCP) as a Simple Method for Allele Typing of HLA-DRB

  • Kang, Joo-Hyun;Kim, Kyeong-Hee;Maeng, Cheol-Young;Kim, Kil-Lyong
    • BMB Reports
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    • 제32권6호
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    • pp.529-534
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    • 1999

  • Asymmetric PCR and single-strand conformation polymorphism (SSCP) methods were combined to analyze human leukocyte antigen (HLA)-DRB allele polymorphism. Asymmetric PCR amplification was applied to generate single-stranded DNA (ssDNA) using the nonradioactive oligonucleotide primers desinged for the polymorphic exon 2 region. The conformational differences of ssDNAs, depending on the allele type, were analyzed by nondenaturing polyacrylamide gel electrophoresis and visualized by ethidium bromide staining. The ssDNAs were clearly separated from double-stranded DNA without interference and obviously migrated depending on their allele type. This method was applied to the genomic DNA either from homozygous or from heterozygous cell lines containing the DR4 allele as template DNA using DR4-specific primers, and satisfying results were obtained. Compared to the standard PCR-SSCP method, this asymmetric PCR-SSCP method has advantages of increased speed, reproducibility, and convenience. Along with PCR-SSP or sequence-based typing, this method will be useful in routine typing of HLA-DRB allele.

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Genetic Differentiation of Phytoplasma Isolates by DNA Heteroduplex Mobility Assay and Single-Strand Conformation Polymorphism Analysis

  • Cha, Byeongjin;Han, Sangsub
    • The Plant Pathology Journal
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    • 제18권6호
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    • pp.308-312
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    • 2002

  • Heteroduplex mobility assay (HMA) and single-strand conformation polymorphism (SSCP) analyses combined with PCR were developed for genetic differentiation of various phytoplasma isolates. In the HMA and SSCP analyses, differences in the mobility shifts and the SSCP band patterns identified three distinct types of phyto-plasmas: Type Ⅰ, jujube witches'-broom (JWB) and ligustrum witches'-broom (LiWB); Type Ⅱ, mulberry dwarf（MD) and sumac witches'-broom (SuWB); and Type Ⅲ, paulownia witches'-broom (PaWB). Results of the sequence analyses revealed that phytoplasmas of JWB and MD had 100％ homology with LiWB and SuWB, respectively. On the other hand, PaWB phyto-plasma had 97.8% homology with MD phytoplasma. The PCR-HMA and SSCP techniques were very useful in determining variations in sequence among several isolates of phytoplasmas. Furthermore, the methods were rapid, economical, highly sensitive, and easy to handle with the gels.

  • https://doi.org/10.5423/PPJ.2002.18.6.308 인용 PDF KSCI

Single-Strand Conformation Polymorphism Analysis by Microchip Electrophoresis for the Rapid Detection of Point Mutation in Human Obesity Gene

  • Kang, Seong-Ho;Jang, Soo-Young;Park, Sang-Kyu
    • Bulletin of the Korean Chemical Society
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    • 제27권9호
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    • pp.1346-1352
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    • 2006

  • We describe an effective method of microchip electrophoresis (ME) based on single strand conformation poly-morphism (SSCP) analysis to rapidly detect the point mutation, Leu72Met, in a human obesity gene. The 207-bp dsDNA in the Leu72Met region, an estimate of the child obesity DNA mutant, was amplified by polymerase chain reaction (PCR) and submitted to a conventional glass microchip analysis with a sieving matrix of 1.75% poly(vinylpyrrolidone) (Mr 1 300 000), 1.0% poly(ethyleneoxide) (Mr 600 000) and 5.0% w/w glycerol. When combined with base stacking (BS) with hydroxide ions, the SSCP-ME provided rapid analysis as well as sensitive detection. The detection sensitivity was effectively enhanced in the OH- concentration range of 0.01-0.025 M NaOH. The sensitivity and speed of this ME-based SSCP methodology for the rapid detection of Leu72Met point mutations makes this an attractive method for diagnosing childhood obesity in a clinical diagnostic laboratory.

  • https://doi.org/10.5012/bkcs.2006.27.9.1346 인용 PDF KSCI

SSCP와 DHPLC에 의한 β2-교감신경수용체 유전자의 돌연변이 분석 (Mutation Analysis in β2-Adrenergic Receptor Gene by Single Strand Conformation Polymorphism (SSCP) and Denaturing High Performance Liquid Chromatography (DHPLC))

  • 박상범;한상만;남윤형;장원철
    • 분석과학
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    • 제17권1호
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    • pp.53-59
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    • 2004

  • 현재 일반적으로 많이 사용되는 single strand conformation polymorphism (SSCP)나 denaturing gradient gel electrophoresis (DGGE)같은 돌연변이 검출법은 많은 시간과 비용, 그리고 노동력이 소모된다는 단점과 실험자의 실험에 대한 숙련도에 의해 실험 결과가 달라지는 한계점을 가지고 있다. 이런 단점들을 보완하기 위하여 ion-pair reversed phase chromatography (IP-RPC)방식을 이용한 denaturing high performance liquid chromatography (DHPLC)방법을 사용하여 기관지 천식 (bronchial asthma)을 조절하는 베타2-교감신경수용체 유전자의 돌연변이를 검출하였다. 80명의 천식 환자의 혈액에서 genomic DNA를 추출하여 중합효소연쇄반응 (polymerase chain reaction)을 이용해 증폭하고, 그 산물을 SSCP와 DHPLC로 분석하였다. 그 결과, 베타2-교감신경수용체 유전자에서 SSCP는 80명의 sample 가운데 19개 (23.75%)의 돌연변이를 검출하였고, DHPLC는 25개 (31.25%)의 변이를 검출하였다. 돌연변이 검출법으로 DHPLC 분석법이 SSCP보다 더 빠르고 효과적인 방법임을 확인하였다.

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