• Title/Summary/Keyword: Single-probe method

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A Study on Solid Electrolyte for New Type Cell (고체 전해질을 이용한 새로운 전지에 관한 연구)

  • Soung Ho Park;Seung Gu Cho;Keu Hong Kim;Yong Bae Kim;Jae Shi Choi
    • Journal of the Korean Chemical Society
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    • v.30 no.6
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    • pp.500-509
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    • 1986
  • For the development of new type cell, a study on new electrolyte, $AgI-Ag_8S_3SO_4$ system has been carried out by using electrical conductivity measurement, DTA and X-ray powder diffraction method. From both X-ray powder diffraction and DTA method, it is clearly known that 20mole% $ Ag_8S_3SO_4$-AgI system forms single phase, having monoclinic structure. It is also found that 20mole% $Ag_8S_3SO_4$-AgI system is purely $ Ag^+ $cation conduction from results of conductivity measurement by applying 4-probe method.

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High-throughput SNP Genotyping by Melting Curve Analysis for Resistance to Southern Root-knot Nematode and Frogeye Leaf Spot in Soybean

  • Ha, Bo-Keun;Boerma, H. Roger
    • Journal of Crop Science and Biotechnology
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    • v.11 no.2
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    • pp.91-100
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    • 2008
  • Melting curve analysis of fluorescently labeled DNA fragments is used extensively for genotyping single nucleotide polymorphism(SNP). Here, we evaluated a SNP genotyping method by melting curve analysis with the two probe chemistries in a 384-well plate format on a Roche LightCycler 480. The HybProbe chemistry is based on the fluorescence resonance energy transfer(FRET) and the SimpleProbe chemistry uses a terminal self-quenching fluorophore. We evaluated FRET HybProbes and SimpleProbes for two SNP sites closely linked to two quantitative trait loci(QTL) for southern root-knot nematode resistance. These probes were used to genotype the two parents and 94 $F_2$ plants from the cross of PI 96354$\times$Bossier. The SNP genotypes of all samples determined by the LightCycler software agreed with previously determined SSR genotypes and the SNP genotypes determined on a Luminex 100 flow cytometry instrument. Multiplexed HybProbes for the two SNPs showed a 98.4% success rate and 100% concordance between repeats two of the same 96 DNA samples. Also, we developed a HybProbe assay for the Rcs3 gene conditioning broad resistance to the frogeye leaf spot(FLS) disease. The LightCycler 480 provides rapid PCR on 384-well plate and allows simultaneous amplification and analysis in approximately 2 hours without any additional steps after amplification. This allowed for a reduction of the potential contamination of PCR products, simplicity, and enablement of a streamlined workflow. The melting curve analysis on the LightCycler 480 provided high-throughput and rapid SNP genotyping and appears highly effective for marker-assisted selection in soybean.

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Single Wall Carbon Nanotube Films Produced by Arc Discharge (아크 방전법으로 성장된 대면적 단일벽 탄소나노튜브 필름)

  • Kang, Young-Jin;Oh, Dong-Hoon;Song, Hye-Jin;Jung, Jin-Yeun;Jung, Hyuk;Cho, You-Suk;Kim, Do-Jin
    • Korean Journal of Materials Research
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    • v.18 no.5
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    • pp.253-258
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    • 2008
  • A simple method to deposit carbon nanotube films uniformly on large area substrates using an arc discharge method is reported in this paper. The arc discharge method was modified to deposit carbon nanotube films in situ on the substrates. The substrates were scanned several times over the arcing point for a uniform film thickness. Deposition was carried out under variable dc bias conditions at 600 torr of $H_2$ gas. The thickness uniformity of the single-wall carbon nanotube films as characterized by a four-point probe was within 30% deviation. The morphology and crystal quality of the single-wall carbon nanotube film were also characterized by field emission scanning electron microscopy and Raman spectroscopy.

Crystal Growth and Photoluminescence Property of Stoichiometric LiNbO3 Single Crystal Fiber by Addition of Nd2O3 (Nd\2O3 첨가에 따른 화학양론조성 LiNbO3 단결정의 성장 및 형광특성)

  • Shur, Joong-Won;Yoon, Seok-Gyu;Lee, Sung-Mun;Yoon, Dae-Ho
    • Journal of the Korean Ceramic Society
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    • v.39 no.10
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    • pp.977-980
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    • 2002
  • Using the micro-pulling down method, $Nd_2O_3$ doped crack-free stoichiometric $LiNbO_3$ single crystals were grown in 1 mm diameter and 30∼35 mm length. The homogeneous distributions of $Nd_2O_3$ concentration were confirmed by the electron probe micro analysis. The absorption and photoluminescence spectra were measured with respect to the $Nd_2O_3$ doping.

Characterization of Electrical Properties and Gating Effect of Single Wall Carbon Nanotube Field Effect Transistor

  • Heo, Jin-Hee;Kim, Kyo-Hyeok;Chung, Il-Sub
    • Transactions on Electrical and Electronic Materials
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    • v.9 no.4
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    • pp.169-172
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    • 2008
  • We attempted to fabricate carbon nanotube field effect transistor (CNT-FET) using single walled carbon nanotube(SWNT) on the heavily doped Si substrate used as a bottom gate, source and drain electrode were fabricated bye-beam lithography on the 500 nm thick $SiO_2$ gate dielectric layer. We investigated electrical and physical properties of this CNT-FET using Scanning Probe Microscope(SPM) and conventional method based on tungsten probe tip technique. The gate length of CNT-FET was 600 nm and the diameter of identified SWNT was about 4 nm. We could observed gating effect and typical p-MOS property from the obtained $V_G-I_{DS}$ curve. The threshold voltage of CNT-FET is about -4.6V and transconductance is 47 nS. In the physical aspect, we could identified SWNT with phase mode of SPM which detecting phase shift by force gradient between cantilever tip and sample surface.

Crystal structure analysis of orthohombic $Sr_{0.6}Ca_{0.4}CuO_2$ compound (사방정계 $Sr_{0.6}Ca_{0.4}CuO_2$ 화합물의 결정구조해석)

  • Park, H.M.;Goetz, D.;Hahn, Th.
    • Korean Journal of Crystallography
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    • v.7 no.1
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    • pp.20-29
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    • 1996
  • Sr0.6Ca0.4CuO2 single crystal has been synthesized by flux method and characterized by the single crystal X-ray diffraction. The compound has the orthorhombic system and the space group is Cmcm(63), lattice parameters are a=3.4645Å, b=16.1417Å, c=3.8727Å. In the (Sr1-xCax)CuO2 compound the limit of Ca from substitution for Sr was determined by the change of bond length. For this, X-ray diffraction, scanning electron microscopy (SEM), energy dispersive X-ray fluorescence (EDAX) and electron probe micro-analysis (EPMA) were used. From the change of Cu-O bond length as the Ca substitution, we concluded the limit of Ca incorporation Xca≒0.73.

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Measurement of Brillouin Gain Spectrum of Optical Fiber Using Direct Optical Frequency Modulation of Laser Diode (레이저 다이오드의 직접 광주파수 변조를 이용한 광섬유의 브릴루앙 이득 스펙트럼 측정)

  • Seo, Min-Sung;Yun, Seung-Chul;Park, Hee-Gap
    • Korean Journal of Optics and Photonics
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    • v.17 no.2
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    • pp.143-148
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    • 2006
  • A new method of measuring the Brillouin gain spectrum of optical fiber is proposed and demonstrated. Unlike existing methods, both the pump light and the counter-propagating probe light are generated from a single laser diode whose optical frequency is directly modulated by injection current modulation. The frequency difference between the pump and the probe is scanned continuously through optical frequency chirp occurring when the injection current is modulated with a square wave. The measured Brillouin gain spectrum of telecom single-mode fiber agrees well with that shown in other literature.

SNP Detection of Biochip Using Electrochemical System (전기화학적 방법에 의한 바이오칩의 SNP 검출)

  • Choi, Yong-Sung;Park, Dae-Hee
    • Proceedings of the KIEE Conference
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    • 2004.07c
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    • pp.2128-2130
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    • 2004
  • High throughput analysis using a DNA chip microarray is powerful tool in the post genome era. Less labor-intensive and lower cost-performance is required. Thus, this paper aims to develop the multi-channel type label-free DNA chip and detect SNP (Single nucleotide polymorphisms). At first, we fabricated a high integrated type DNA chip array by lithography technology. Various probe DNAs were immobilized on the microelectrode array. We succeeded to discriminate of DNA hybridization between target DNA and mismatched DNA on microarray after immobilization of a various probe DNA and hybridization of label-free target DNA on the electrodes simultaneously. This method is based on redox of an electrochemical ligand.

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펌프-촉침 방법을 이용한 피코초 순간 흡광 속도분광기 제작

  • 장두전
    • Korean Journal of Optics and Photonics
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    • v.4 no.3
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    • pp.347-353
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    • 1993
  • A picosecond transient absorption kinetic spectrometer using a pump-probe method is presented and compared with other methods of picosecond transient absorption measurements. This constructed kinetic spectrometer detects the transient transmittance of sample using a lock-in amplifier as a function of delay time between pump and probe pulses generated from a picosecond mode-locked cw dye laser. Typical transient absorption and ground state bleach recovery kinetic profiles measured with this spectrometer are shown. Excellent kinetic curves of transient absorption or ground state bleach recovery may be obtained at single wavelengths with this spectrometer.

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Design, Fabrication and Evaluation of Diamond Tip Chips for Reverse Tip Sample Scanning Probe Microscope Applications (탐침과 시편의 위치를 역전시킨 주사 탐침 현미경용 다이아몬드 탐침의 제작 및 평가)

  • Sugil Gim;Thomas Hantschel;Jin Hyeok Kim
    • Korean Journal of Materials Research
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    • v.34 no.2
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    • pp.105-110
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    • 2024
  • Scanning probe microscopy (SPM) has become an indispensable tool in efforts to develop the next generation of nanoelectronic devices, given its achievable nanometer spatial resolution and highly versatile ability to measure a variety of properties. Recently a new scanning probe microscope was developed to overcome the tip degradation problem of the classic SPM. The main advantage of this new method, called Reverse tip sample (RTS) SPM, is that a single tip can be replaced by a chip containing hundreds to thousands of tips. Generally for use in RTS SPM, pyramid-shaped diamond tips are made by molding on a silicon substrate. Combining RTS SPM with Scanning spreading resistance microscopy (SSRM) using the diamond tip offers the potential to perform 3D profiling of semiconductor materials. However, damage frequently occurs to the completed tips because of the complex manufacturing process. In this work, we design, fabricate, and evaluate an RTS tip chip prototype to simplify the complex manufacturing process, prevent tip damage, and shorten manufacturing time.