• 대한원격탐사학회:학술대회논문집
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• 대한원격탐사학회 2007년도 Proceedings of ISRS 2007
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• pp.247-249
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• 2007

Crown parameters are important roles in tree species identification, because the canopy is the aggregate of all the crowns. However, crown measurements with spaceborne image data have remained more difficult than on aerial photographs since trees show more structural detail at higher resolutions. This recognized problem led to the initiation of the research to determine if high resolution satellite image data could be used to identify and classify single tree species. In this paper, shape parameters derived from pixel-based crown area measurements and texture features derived from GLCM parameters in QuickBird image were tested and compared for individual tree species identification. As expected, initial studies have shown that the crown parameters and the canopy texture parameters provided a differentiating method between coniferous trees and broad-leaved trees within the compartment(less than forest stand) for single extraction from spaceborne high resolution image.

• Proceedings of the National Institute of Ecology of the Republic of Korea
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• 제3권4호
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• pp.212-220
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• 2022

DNA markers have been studied and used intensively to identify plant species based on molecular approaches. The genus Medicago belongs to the family Fabaceae and contains 87 species distributed from the Mediterranean to central Asia. Five species of Medicago are known to be distributed in South Korea; however, their morphological characteristics alone cannot distinguish the species. In this study, we analyzed the phylogenetic relationships using collected five species of Medicago from South Korea and 44 taxa nucleotide information from NCBI. The constructed phylogenetic tree using gibberellin 3-oxidase 1 and tRNA^Lys (UUU) to maturase K gene sequences showed the monophyly of the genus Medicago, with five species each forming a single clade. These results suggest that there are five species of Medicago distributed in South Korea. In addition, we designed polymerase chain reaction primers for species-specific detection of Medicago by comparing the plastid sequences. The accuracy of the designed primer pairs was confirmed for each Medicago species. The findings of this study provide efficient and novel species identification methods for Medicago, which will assist in the identification of wild plants for the management of alien species and living modified organisms.

• 대한원격탐사학회지
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• 제24권3호
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• pp.267-272
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• 2008

Tree species-specific estimates with spacebome high-resolution imagery improve estimation of forest biomass which is needed to predict the long term planning for the sustainable forest management(SFM). This paper is a contribution to develop crown distinguishing coniferous species, Pinus densiflora and Pinus koraiensis, from QuickBird imagery. The proposed feature analysis derived from shape parameters and first and second-order statistical texture features of the same test area were compared for the two species separation and delineation. As expected, initial studies have shown that both formfactor and compactness shape parameters provided the successful differentiating method between the pine species within the compartment for single crown identification from spaceborne high resolution imagery. Another result revealed that the selected texture parameters - the mean, variance, angular second moment(ASM) - in the infrared band image could produce good subset combination of texture features for representing detailed tree crown outline.

• Journal of the Korean Wood Science and Technology
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• 제33권2호통권130호
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• pp.1-7
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• 2005

Tree species of wooden structural members of the Beomeo Temple were identified based on light and scanning electron microscopic characters in the present study. Of 10 structural members, 9 softwoods and 1 hardwood were identified. Among softwood members, 7 belonged to hard pine of the Sylvestris section, and the remaining 2 to hard pine of other than the Sylvestris section and hemlock of the genus Tsuga, respectively. A single hardwood member was identified as white oak of the Prinus section under the subgenus Lepidobalanus.

• BMB Reports
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• 제39권4호
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• pp.361-370
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• 2006

Genetic variation and molecular phylogeny of 22 taxa representing 14 extant species and 3 unidentified taxa of Boesenbergia in Thailand and four outgroup species (Cornukaempferia aurantiflora, Hedychium biflorum, Kaempferia parviflora, and Scaphochlamys rubescens) were examined by sequencing of 3 chloroplast (cp) DNA regions (matK, psbA-trnH and petA-psbJ). Low interspecific genetic divergence (0.25-1.74%) were observed in these investigated taxa. The 50% majority-rule consensus tree constructed from combined chloroplast DNA sequences allocated Boesenbergia in this study into 3 different groups. Using psbA-1F/psbA-3R primers, an insertion of 491 bp was observed in B. petiolata. Restriction analysis of the amplicon (380-410 bp) from the remaining species with Rsa I further differentiated Boesenbergia to 2 groupings; I (B. basispicata, B. longiflora, B. longipes, B. plicata, B. pulcherrima, B. tenuispicata, B. thorelii, B. xiphostachya, Boesenbergia sp.1 and Boesenbergia sp.3; phylogenetic clade A) that possesses a Rsa I restriction site and II (B. curtisii, B. regalis, B. rotunda and Boesenbergia sp.2; phylogenetic clade B and B. siamensis; phylogenetic clade C) that lacks a restriction site of Rsa I. Single nucleotide polymorphism (SNP) and indels found can be unambiguously applied to authenticate specie-origin of all investigated samples and revealed that Boesenbergia sp.1, Boesenbergia sp.2 and B. pulcherrima (Mahidol University, Kanchanaburi), B. cf. pulcherrima1 (Prachuap Khiri Khan) and B. cf. pulcherrima2 (Thong Pha Phum, Kanchanaburi) are B. plicata, B. rotunda and B. pulcherrima, respectively. In addition, molecular data also suggested that Boesenbergia sp.3 should be further differentiated from B. longiflora and regarded as a newly unidentified Boesenbergia species.

• 한국산림과학회지
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• 제96권5호
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• pp.515-519
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• 2007

An imperfect fungus Fusiococcum species was isolated from Quercus dentata. A naturally infected Daimyo oak tree was collected and showed elongate wounds on the stem. The fungal cultures were initially white and cottony, and later turned dark gray. Numerous solitary pycnidia were developed on the medium surface, and typically spherical. Yellowish conidial masses were exuded from pycnidia on the culture plates. Conidial masses were swollen and measured as approximately 100 to $300{\mu}m$ in length. It appeared that conidia were usually held together in globose to oval drops. Conidia were hyaline, single-celled (nonseptate), ellipsoid to fusoid, and measured as approximately $8.0{\times}2.7{\mu}m$. Based on these cultural and morphological characteristics, the fungal isolate was identified as a species of Fusicoccum Corda. To preserve and examine fungal spores exuded from pycnidia on the medium surface, a vapor fixation procedure for scanning electron microscopy was employed in this study. The specimens were exposed to the vapor of 2% (v/v) glutaraldehyde and 2% (w/v) osmium tetroxide each for 2 h. With the vapor fixation we obtained excellent retention of conidial masses in this study. The simple and versatile procedure for demonstrating fungal spores and their exudation from fruiting bodies would facilitate characterization of diverse pathological and environmental isolates as they are in native environments.

• Animal Systematics, Evolution and Diversity
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• 제34권3호
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• pp.162-167
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• 2018

To confirm the genetic identification and phylogenetic relationships of unidentified 6 baleen whales by-caught from 2002 to 2016, a partial sequence of approximately 500 base pair (bp) of the mitochondrial DNA (mtDNA) control region was analyzed and compared to published sequence from Genbank. Our results indicated that the two individuals among 6 specimens are clustered with Omura's whale clade through phylogenetic analysis, which had only a single haplotype. Omura's whale was reclassified as a new species in 2003 and they had not been previously reported in Korean waters. This study firstly revealed existence of Omura's whale in Korean waters by molecular analysis based on mtDNA control region.

• 대한원격탐사학회지
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• 제7권2호
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• pp.149-163
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• 1991

This paper discusses the capabilities of airborne remotely sensed data to detect and classify forest damades. In this work the AMS (Aircraft Multiband Scanner) was used to obtain digital imagery at 300m altitude for forest damage inventory in the Black Forest of Germany. MSS(Multispectral Scanner) digital numbers were converted to spectral emittance and radiance values in 8 spectral bands from the visible to the thermal infrared and submitted to a maximum-likelihood classification for : (1) tree species ; and. (2) damage classes. As expected, the resulted, the results of MSS data with high spatial resolution 0.75m$\times$0.75m enabled the detection and identification of single trees with different damages and were nearly equivalent to the truth information of ground checked data.

• 한국수산과학회지
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• 제43권5호
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• pp.482-488
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• 2010

Three specimens of Cynoglossidae larvae were collected from the southern Korean Sea in May and August of 2009, and were identified using morphological and molecular analysis. Specimens were divided into two groups based on the number of elongated dorsal fin rays on the top of the head: Cynoglossidae sp. A was defined as having two elongated dorsal fin rays, while Cynoglossidae sp. B possessed a single elongated dorsal fin ray. One specimen of Cynoglossidae sp. A, a post-larva with a notochord length (NL) of 5.8 mm was thought to be a Cynoglossus joyneri larva based on the presence of 115 dorsal pterogiophores, 85 anal pterogiophores, and 50 myomeres. Two specimens of Cynoglossidae sp. B, a 4.1 mm NL larva and a 11.3 mm NL juvenile, were thought to be Cynoglossus abbreviatus based on the presence of yolk in the former and 133 dorsal fin rays, 105 anal fin rays, and 63 myomeres in the latter. To test this morphology-based identification, molecular analysis was conducted using 419-422 bp of mitochondrial DNA 16S rRNA. Cynoglossidae sp. A was clearly matched to a Cynoglossus joyneri adult (d=0.000) and Cynoglossidae sp. B clustered closely with Cynoglossus abbreviatus adults (d=0.002). A neighbor-joining tree supported this robust relationship (bootstrap value=100%). Therefore, these molecular data validate the morphological identification of the two Cynoglossidae larval species.

• 한국산림과학회지
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• 제88권1호
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• pp.11-17
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• 1999

유묘상태에서 잡종을 판정하기 위해 수령에 따른 잎과 모용(毛茸)의 형태를 조사하였다. 졸참나무, 떡갈나무, 갈참나무에서 잎의 크기는 1년생묘에서 가장 작고, 수령의 증가에 따라서 커지는 경향을 보였다. 잎의 길이, 잎의 넓이, 잎자루 길이, 결각의 수, 결각의 깊이, 형상비, 잎자루 비율에 관하여서도 수령에 따른 차이가 관찰되었다. 이 때문에 1, 2년생의 유령기에 잎의 크기, 형태만으로 종을 판정하기는 어려웠다. 또한 잎 뒷면의 모용(毛茸)의 종류, 형상, 발생 밀도 등은 종에 따라서, 같은 종 중에서도 수령에 따라서 현저하게 차이를 보였다. 졸참나무의 성목은 소형성상모밀생(小型星狀毛密生) 장모소생형(長毛疎生型), 떡갈나무의 성목은 대형성상모밀생(大型星狀毛密生)~소생형(疎生型), 갈참나무의 성목은 소형성상모밀생형(小型星狀毛密生型), 일본 물참나무의 성목은 무모(無毛)~소형성상모산생형(小型星狀毛散生型)으로 성목에서는 모용(毛茸)으로 종의 판정이 가능하였다. 1, 2년생의 묘목에서는 모용(毛茸)의 발생이 좋지 않으며 졸참나무, 떡갈나무에서는 2년생 일부와 3년생에서 모용(毛茸)에 의해 종의 분류가 가능하였지만, 갈참나무 일본 물참나무에서는 유령기에 모용(毛茸)만으로서 종을 판정하기는 곤란하였다.