• Molecules and Cells
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• v.42 no.4
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• pp.356-362
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• 2019

The binding of MS2 bacteriophage coat protein (MCP) to MS2 binding site (MBS) RNA stem-loop sequences has been widely used to label mRNA for live-cell imaging at single-molecule resolution. However, concerns have been raised recently from studies with budding yeast showing aberrant mRNA metabolism following the MS2-GFP labeling. To investigate the degradation pattern of MS2-GFP-labeled mRNA in mammalian cells and tissues, we used Northern blot analysis of ${\beta}$-actin mRNA extracted from the Actb-MBS knock-in and $MBS{\times}MCP$ hybrid mouse models. In the immortalized mouse embryonic cell lines and various organ tissues derived from the mouse models, we found no noticeable accumulation of decay products of ${\beta}$-actin mRNA compared with the wild-type mice. Our results suggest that accumulation of MBS RNA decay fragments does not always happen depending on the mRNA species and the model organisms used.

• Journal of Multimedia Information System
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• v.9 no.2
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• pp.75-86
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• 2022

The existing image quality assessment (IQA) datasets have a small number of samples. Some methods based on transfer learning or data augmentation cannot make good use of image quality-related features. A No Reference (NR)-IQA method based on multi-task training and quality awareness is proposed. First, single or multiple distortion types and levels are imposed on the original image, and different strategies are used to augment different types of distortion datasets. With the idea of weak supervision, we use the Full Reference (FR)-IQA methods to obtain the pseudo-score label of the generated image. Then, we combine the classification information of the distortion type, level, and the information of the image quality score. The ResNet50 network is trained in the pre-train stage on the augmented dataset to obtain more quality-aware pre-training weights. Finally, the fine-tuning stage training is performed on the target IQA dataset using the quality-aware weights to predicate the final prediction score. Various experiments designed on the synthetic distortions and authentic distortions datasets (LIVE, CSIQ, TID2013, LIVEC, KonIQ-10K) prove that the proposed method can utilize the image quality-related features better than the method using only single-task training. The extracted quality-aware features improve the accuracy of the model.

• BMB Reports
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• v.30 no.6
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• pp.397-402
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• 1997

A thiol-specific spin label was attached to cysteine-102 of yeast cytochrome c and electron paramagnetic resonance (EPR) spectra were measured as a function of added cytochrome c oxidase concentration. The intensity decreased due to line broadening as cytochrome c formed a complex with cytochrome c oxidase and reached a minimum when the ratio of cytochrome c to cytochrome c oxidase became one. Replacement of either Lys-72 or Lys-87 of cytochrome c by Glu did not result in a significant change in binding affinity. Interestingly the K72E mutant, unlike K87E, had a much lower rate of electron transfer than the wild type. These results indicate that many positively charged residues as a group participate in complex formation but Lys-72 might be important for cytochrome c to be locked in an orientation for an efficient electron transfer. A stoichiometry of 1 was also confirmed by optical absorption of the cytochrome c-cytochrome c oxidase complex which had been run through a gel chromatography cloumn to remove unbound cytochrome c. The EPR spectrum of this 1:1 complex, however, was a mixture of two components. This explains a biphasic kinetics for a single binding site on cytochrome c oxidase without invoking conformational transition.

• Journal of the Institute of Electronics Engineers of Korea SC
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• v.48 no.2
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• pp.86-90
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• 2011

The effects of cations are very important in field-effect transistors (FETs) type DNA sensors detecting the intrinsic negative charge between single-stranded DNA and double-stranded DNA without labeling, because the intrinsic negative charge of DNA is neutralized by cations in electrolyte solution. We consider the Debye length, which depends on the concentration of cations in solution, to detect DNA hybridization based on the intrinsic negative charge of DNA. The Debye length is longer in buffer solution with a lower concentration of NaCl and the intrinsic negative charge of DNA is more effective on the channel surface in longer Debye length solution. The shifts in the gate voltage by DNA hybridization with complementary target DNA are 21 mV in 1 mM NaCl buffer solution, 7.2 mV in 10 mM NaCl buffer solution, and 5.1 mV in 100 mM NaCl buffer solution. The sensitivity of FETs to detect DNA hybridization based on charge detection without labeling depends on the Debye length.

• Journal of the Korean Institute of Telematics and Electronics
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• v.26 no.2
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• pp.140-146
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• 1989

An efficient quantization and encoding of BTC (Block Truncation Coding) parameters {($Y_{\alpha},\;Y_{\beta}),\;P_{{\beta}/{\beta}}$} are investigated, In our algorithm 4${\times}$4 blocks are classified into flat or edge block. While edge block is represented by two approximation level $Y_{\alpha},\;Y_{\beta}$ with label plane $P_{{\beta}/{\beta}}$, flat block is represented by single approximation level Y. The approximation levels Y, $Y_{\alpha}$ and $Y_{\beta}$ are encoded by predictive quatization specially designed, and the label plane $P_{{\beta}/{\beta}}$ is tried to be encoded using stored 32 reference plantes. The performance of the proposed scheme has appeared comparable to much more complex transform coding in terms of SNR, although it requires more study on the representation of small slope in background.

• The Transactions of the Korean Institute of Electrical Engineers
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• v.34 no.8
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• pp.301-307
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• 1985

The first four generations of computers are all based on a single basic design: the Von Neuman Processor, which is sequential and does one operation at a time. Efforts to develop concurrent or parallel computers have been carried on for many years. Data flow approach is significant in these efforts to make high speed parallel machines and expected a great deal of parallelism. In this paper we propose a preliminary data Flow Machine Model operating asynchronously on the base of Node Labelling. We introduce a concept of Node Labeling for this purpose which is relevant to the Data dependency and Parallelism. And we explain how the Node Tokens are fired in the proposed system.

• The Magazine of the IEIE
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• v.36 no.11
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• pp.66-82
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• 2009

Probabilistic atlases for the human brain structure are more suitable than single brain atlases for representing population anatomy. In this study, we hypothesized the group-specific probabilistic atlas for accurate characteristic feature coding. Our proposed method for a new group comparison study, using a subpopulation specific probabilistic atlas, was based on this hypothesis. A knowledge-based automatic labeling technique using nonlinear registration was applied to encode group-specific regional probabilistic information. Direct atlas-based comparison using volume counting above the probability threshold, distance measurement and correlation analysis were performed based on the probabilistic atlas. Here, we applied this method for comparison between Korean and occidental groups. The results showed that this method could provide simple but intuitive regions of interest-based group analysis for the entire cortex area.

• Journal of the Korean Society of Clothing and Textiles
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• v.28 no.3_4
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• pp.403-413
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• 2004

The purpose of this study was to investigate to wearing practices and consumer needs for stockings. 503 subjects were surveyed in 2002. Data were analyzed by frequency, $\chi$$^2$, ANONA(LSD) and factor analysis. The results were as follows; 1) Many subjects considered the colors, but a very few of them considered the components and handling signs when purchase their stockings. These tendencies show meaningful differences according to their ages, jobs and the status of whether they are married or single. The wearing rates of stockings show differences according to their physical characteristics and social demographical variables. 2) They demand that the exact fiber contents and the mixture ratios which the stockings have should be indicated, and also want the sizes of the stockings to be indicated by three steps according to their body sizes, and they ask that the stockings have more various colors. They required that the stockings should have good ventilation, durability, warmth-keeping, hygroscopicity and elasticity in leg parts, and anti static electricity or bacteria.

• Radiation Oncology Journal
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• v.8 no.2
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• pp.137-144
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• 1990

The filter elution technique was used to assay Co-60 $\gamma$ ray-induced DNA single-strand breaks(SSB) in EL 4 mouse leukemia cell and mouse spleen lymphocyte. The lymphocytes were stimulated with lipopolysaccharide (LPS, 20 $\mug/ml$) to label [${^3}H$] thymidine. EL 4 cells and lymphocytes in suspension were exposed at $0^{\circ}C$ to 0 Gy, 1 Gy, 5 Gy,10 Gy of Co-60 radiation and elution procedure was performed at PH 12.1. The number of DNA single-strand breaks increased with increasing doses of $\gamma$ rays. The strand scission factor (SSF) was estimated in each experiment (eluted volume 21 ml). The slope for EL 4 cells was $0.01301\pm0.00096\;Gy^{-1}(n=5)$ and the slope for lymphocytes was $0.01097\pm0.00091\;Gy^{-1}(n=5)$. The slopes were significantly different (P<0.005). Thus EL 4 cells were more sensitive to induction of DNA SSB by ionizing radiation than lymphocytes.

• Journal of Periodontal and Implant Science
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• v.47 no.5
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• pp.312-327
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• 2017

Purpose: This study assessed marginal bone remodeling and soft tissue esthetics after the loading of single bone-level implants in the anterior maxilla. Methods: An open, single-arm observational clinical trial with 3 years of follow-up was performed, including 22 implants. The patients presented with a single tooth gap in the anterior maxilla (tooth positions 14-24), with natural or restored adjacent teeth. An implant was placed at least 8 weeks post-extraction and healed submerged for 6 weeks. After the second-stage operation, a fixed provisional prosthesis was provided. The final restoration was placed 6 months after the provisional restoration. The time of the provisional crown connection was considered to be the baseline in this study. Esthetic parameters and the marginal bone level were assessed at 6, 12, 24, and 36 months. Results: All implants were well integrated in the bone. A statistically significant increase was found in the mean implant stability quotient between the time of the provisional prosthesis and the time of the final prosthesis. Most implants (95.5%) revealed marginal bone resorption (<0.5 mm), and just 1 implant (4.5%) showed a change of 2.12 mm from baseline to 36 months (mean $0.07{\pm}0.48mm$), while the crestal bone level decreased significantly, from $2.34{\pm}0.93mm$ at baseline to $1.70{\pm}1.10mm$ at 36 months. The facial gingival margin and papilla were stable and the esthetic scores indicated high patient and dentist satisfaction. Conclusions: Platform-switching bone-level implants placed in maxillary single-tooth gaps resulted in successful osseointegration with minimal marginal bone resorption. The peri-implant soft tissue was also esthetically satisfying and stable.